##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-173_CGAGGCTG-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2438877	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41060455283313	34.0	31.0	34.0	31.0	34.0
2	32.59518048675682	34.0	31.0	34.0	31.0	34.0
3	32.70673797817602	34.0	31.0	34.0	31.0	34.0
4	36.23581099005813	37.0	35.0	37.0	35.0	37.0
5	35.953467108017335	37.0	35.0	37.0	35.0	37.0
6	36.03925536220154	37.0	36.0	37.0	35.0	37.0
7	36.03502923681678	37.0	36.0	37.0	35.0	37.0
8	36.05967459613584	37.0	36.0	37.0	35.0	37.0
9	37.92899354908017	39.0	38.0	39.0	35.0	39.0
10-14	38.10583379153603	39.2	38.2	39.4	35.2	39.4
15-19	39.22094062144175	40.0	39.0	41.0	36.0	41.0
20-24	39.147235469439416	40.0	39.0	41.0	36.0	41.0
25-29	38.94105639603801	40.0	38.4	41.0	35.4	41.0
30-34	38.723054504183686	40.0	38.0	41.0	34.8	41.0
35-39	38.47456120173342	40.0	38.0	41.0	34.0	41.0
40-44	38.206746465688916	40.0	38.0	41.0	33.6	41.0
45-49	38.01362463133647	40.0	37.6	41.0	33.0	41.0
50-54	37.9539482310916	40.0	37.0	41.0	33.0	41.0
55-59	37.5591676004981	39.4	36.2	41.0	32.8	41.0
60-64	36.98855760253592	38.8	35.2	40.6	31.8	41.0
65-69	36.23974730992994	37.6	35.0	40.0	31.0	41.0
70-74	35.65880468756727	36.4	34.8	39.0	30.8	40.8
75-79	34.461752273689896	35.2	33.4	37.4	29.4	39.2
80-84	34.2694144067126	35.0	34.0	36.4	30.0	38.2
85-89	33.64765020950216	35.0	34.0	35.4	29.6	36.6
90-94	33.23517176142955	35.0	33.8	35.0	29.0	36.0
95-99	32.955885598166695	35.0	33.0	35.0	29.0	35.2
100-101	32.588527219699884	35.0	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	13.0
14	35.0
15	112.0
16	262.0
17	563.0
18	908.0
19	1514.0
20	2248.0
21	3210.0
22	4393.0
23	6128.0
24	8341.0
25	10678.0
26	13649.0
27	17497.0
28	21970.0
29	27777.0
30	35268.0
31	44604.0
32	57118.0
33	77617.0
34	111174.0
35	176093.0
36	316435.0
37	595896.0
38	749005.0
39	156240.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.209499290042096	23.389494427148232	10.897474534386113	24.50353174842356
2	15.435439080520103	23.712988949379067	39.348379149313786	21.503192820787042
3	15.927330488581426	27.855689319305565	32.90723558424636	23.30974460786665
4	11.410251521499443	18.44685074319041	43.91008648652638	26.232811248783765
5	10.952745874433193	39.27848759900561	38.645696359431	11.1230701671302
6	25.486812168059313	43.98479300104105	19.398805269802455	11.129589561097177
7	23.4953628247755	33.882356510803945	27.043840259266865	15.578440405153685
8	21.291725658981573	38.08568451791542	24.328533173259657	16.29405664984335
9	23.01313268360807	14.62976607676402	25.115944756541637	37.24115648308627
10-14	20.581128117572145	25.801547187496542	32.72130574850638	20.89601894642493
15-19	19.93587958910125	31.331804470059442	28.60318125476403	20.129134686075275
20-24	19.935230426397368	29.932732539268734	30.05535534371534	20.076681690618557
25-29	19.921576462702806	30.283537307035335	29.658525926862765	20.136360303399094
30-34	19.797935528781075	30.14000699059026	29.730781904573355	20.33127557605531
35-39	19.924659825172135	30.307499716758553	29.683318419915523	20.084522038153793
40-44	19.649314047071815	29.938981280870465	30.145721769048116	20.2659829030096
45-49	19.64795811538944	29.661748557123346	30.324613608363272	20.36567971912395
50-54	19.221595128910394	29.309019346322774	30.801812841909804	20.66757268285703
55-59	19.159648671162714	29.247333210931682	30.79020931174667	20.802808806158936
60-64	18.807733239930453	28.874371403293765	30.737670249599176	21.580225107176602
65-69	19.10333827590094	28.99628085050666	29.912039579481952	21.988341294110448
70-74	19.1449087339532	28.829196606205336	29.474398106612913	22.55149655322855
75-79	19.686200948858055	28.92366287452015	28.805033066029466	22.58510311059232
80-84	19.85019641854338	28.739455788114643	28.42884518492471	22.981502608417262
85-89	20.067537928579355	28.47287571786111	28.35082425943106	23.108762094128465
90-94	20.1297446700471	27.8781286431541	28.65471706654298	23.337409620255823
95-99	20.289332369591893	27.445850638773926	28.635717051679404	23.629099939954777
100-101	20.216017586793587	26.606749709035988	29.375915252746605	23.801317451423824
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.71303103846566
2	36.93863190130715
3	39.23707509644807
4	37.64306277028321
5	22.075816041563392
6	36.61640172915649
7	39.07380322992918
8	37.585782308824925
9	60.25428916669434
10-14	41.47714706399708
15-19	40.06501427517653
20-24	40.011912117015925
25-29	40.057936766101896
30-34	40.12921110483638
35-39	40.009181863325935
40-44	39.91529695008142
45-49	40.01363783451339
50-54	39.88916781176742
55-59	39.96245747732165
60-64	40.38795834710706
65-69	41.09167957001139
70-74	41.69640528718175
75-79	42.27130405945038
80-84	42.83169902696064
85-89	43.17630002270782
90-94	43.46715429030292
95-99	43.91843230954667
100-101	44.01733503821741
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	4.0
7	8.5
8	11.5
9	24.0
10	40.0
11	54.5
12	94.0
13	158.0
14	263.0
15	413.0
16	638.5
17	1000.5
18	1551.5
19	2334.0
20	3485.0
21	5155.0
22	7324.0
23	10121.5
24	13530.5
25	17645.5
26	22547.5
27	28015.0
28	34115.5
29	40469.5
30	47664.5
31	55519.0
32	63166.5
33	71654.0
34	81118.0
35	91587.0
36	103895.0
37	114890.5
38	121672.0
39	123429.0
40	120052.5
41	114347.0
42	107272.0
43	100418.5
44	95452.0
45	89920.5
46	84461.5
47	79333.5
48	72688.0
49	64845.5
50	57056.0
51	50736.0
52	45029.0
53	39698.5
54	35108.5
55	30967.5
56	27183.5
57	23699.5
58	20343.0
59	17592.5
60	15237.5
61	13084.5
62	11273.0
63	9324.0
64	7683.0
65	6603.5
66	5647.0
67	4834.0
68	4234.0
69	3817.5
70	3469.0
71	2897.0
72	2412.5
73	2043.5
74	1664.5
75	1322.0
76	989.0
77	738.5
78	550.5
79	385.5
80	282.0
81	195.5
82	125.5
83	90.5
84	61.0
85	36.5
86	22.5
87	10.5
88	9.5
89	10.5
90	6.0
91	2.0
92	1.0
93	2.0
94	2.5
95	1.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011521696256104757
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011480693778325023
20-24	6.478391489197692E-4
25-29	0.017442454047498092
30-34	0.006978621718110425
35-39	0.042134146166452835
40-44	0.05049865163351821
45-49	0.051113688800214205
50-54	0.05108088681799041
55-59	0.04105988124862385
60-64	0.05185993389580533
65-69	0.04132229710641414
70-74	0.0029931808779204527
75-79	0.001213673342280074
80-84	1.3940842445108958E-4
85-89	0.0028537724534693633
90-94	0.002304339251220951
95-99	0.0019927204200949865
100-101	6.150371666959834E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2438877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.77509156859441
#Duplication Level	Relative count
1	100.0
2	7.690996230275247
3	2.77646226802301
4	2.0408292131567816
5	1.5988179355547558
6	1.3209822753477682
7	1.227528462369054
8	0.9256979042350995
9	0.8335069806209627
10++	7.874115188138943
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	775260	6.253706	17.774715	90-94
GAGCC	505725	4.079477	9.474255	85-89
CGAGC	498725	4.0230107	9.533708	85-89
CTCCG	541300	3.9639611	8.545114	75-79
GTCTC	746945	3.9461687	6.5274916	60-64
CTGTC	718395	3.7953365	6.3059044	65-69
AGCCC	484200	3.617589	8.688378	80-84
GCCCA	478490	3.574928	8.805025	80-84
TCTCT	990675	3.4971814	5.3780665	60-64
TCTCC	677935	3.3172596	6.3157096	80-84
CTCTT	927730	3.2749794	5.0782	60-64
TGTCT	842930	3.212729	5.0325727	65-69
CCACG	427895	3.196919	8.574894	90-94
CCCAC	461585	3.1941142	8.117207	90-94
ATCTC	879865	3.1688817	7.8629613	95-97
GGCTG	370635	3.1639464	10.438885	95-97
CGAGG	362070	3.153394	10.161399	90-94
GAGGC	351280	3.05942	10.27716	95-97
TCCGA	566695	3.0544946	6.570005	85-89
ACACA	791860	2.9685497	6.465338	6
GACCG	359520	2.9001012	9.161342	85-89
CGAGA	473765	2.8128827	7.3678	85-89
GAGAC	468855	2.7837307	7.3666487	85-89
ACGAG	453635	2.6933646	7.1663494	95-97
TACAC	730820	2.6853654	7.59486	5
AGAAG	576655	2.5200162	5.1954026	5
CACGA	443380	2.4381986	6.429491	95-97
AGACC	424035	2.3318183	6.603799	85-89
AGGCT	391740	2.2797308	7.1661963	95-97
ACCGA	378060	2.0789964	6.3812838	90-94
GCTGA	355345	2.0679302	6.9385786	95-97
TATAC	765045	2.0280354	5.5935683	5
CAAAG	488580	1.9775491	5.0926104	4
CTTTG	481215	1.8340946	7.9462557	9
AGAGA	416415	1.8197578	5.169976	8
GAGAG	265705	1.7032721	5.70959	7
TCTCG	302055	1.5957801	5.556261	90-94
TTGAG	335775	1.4097095	5.084933	9
CTCGT	263900	1.3942041	5.421005	90-94
GTCTT	338735	1.291049	5.8865466	1
AAGAC	303990	1.2304131	5.65584	5
GAGTC	208435	1.2129874	6.0664635	9
TGCCG	153225	1.2114812	7.710765	95-97
TATGA	418815	1.1986889	5.609085	4
GCCGT	151095	1.1946402	7.407905	95-97
GATTG	279480	1.1733619	5.571441	7
ATGCC	216710	1.1680703	5.5396705	95-97
GTGTT	279945	1.151996	5.5933223	1
TCATA	422410	1.1197543	5.512033	2
GACTT	287905	1.1195277	5.460038	7
CCGTC	151165	1.1069874	6.188924	95-97
ACATG	262235	1.0403495	5.043509	8
TGGAC	173265	1.0083157	6.941163	5
GTGTA	236520	0.9929997	9.271346	1
GTCCA	173770	0.9366229	6.403605	1
GGACT	155395	0.9043212	6.0213184	6
GTATA	248675	0.7117318	6.8297696	1
>>END_MODULE