##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-171_CGAGGCTG-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1270236	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.734649309262217	33.0	31.0	34.0	30.0	34.0
2	31.926929326518852	33.0	31.0	34.0	30.0	34.0
3	32.07374771302341	34.0	31.0	34.0	30.0	34.0
4	35.604035785476086	37.0	35.0	37.0	33.0	37.0
5	35.4784480994083	37.0	35.0	37.0	33.0	37.0
6	35.61794343728252	37.0	35.0	37.0	33.0	37.0
7	35.63325004172453	37.0	35.0	37.0	33.0	37.0
8	35.683757191577	37.0	35.0	37.0	33.0	37.0
9	37.50024877266902	39.0	38.0	39.0	35.0	39.0
10-14	37.634736852049535	39.2	37.2	39.4	34.6	39.4
15-19	38.700104390050356	40.0	38.2	41.0	34.6	41.0
20-24	38.60425275303172	40.0	38.0	41.0	34.0	41.0
25-29	38.4310410034041	40.0	38.0	41.0	34.0	41.0
30-34	38.17327299808854	40.0	38.0	41.0	33.4	41.0
35-39	37.871311157926556	40.0	38.0	41.0	32.8	41.0
40-44	37.840014768909086	40.0	37.8	41.0	32.8	41.0
45-49	37.6883983763647	40.0	37.2	41.0	32.4	41.0
50-54	36.99013033798444	39.2	36.4	40.2	31.4	40.6
55-59	37.61993566549838	40.0	36.8	41.0	32.4	41.0
60-64	37.17934289376147	39.2	35.6	41.0	31.8	41.0
65-69	36.52122786631775	38.2	35.0	40.4	31.0	41.0
70-74	35.70430880560777	36.8	35.0	39.4	30.2	41.0
75-79	34.74447724674785	35.6	34.0	38.0	29.2	39.6
80-84	33.80966198407226	35.0	34.0	36.6	28.8	38.4
85-89	32.99380004975453	35.0	33.0	35.6	27.0	36.8
90-94	32.35720905406554	35.0	33.0	35.0	25.6	36.0
95-99	31.797690665356676	35.0	32.0	35.0	24.2	35.2
100-101	30.873781722451575	34.5	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	31.0
7	108.0
8	250.0
9	373.0
10	436.0
11	559.0
12	760.0
13	1021.0
14	1379.0
15	1550.0
16	1826.0
17	2216.0
18	2439.0
19	2818.0
20	3346.0
21	3811.0
22	4466.0
23	5224.0
24	6009.0
25	7185.0
26	8456.0
27	10080.0
28	12224.0
29	14913.0
30	18285.0
31	23502.0
32	30622.0
33	42051.0
34	60121.0
35	92543.0
36	161653.0
37	298932.0
38	371647.0
39	79347.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62894288351463	23.547201194560216	10.729305472862501	24.094550449062655
2	14.959552812875057	23.83296949765476	40.07846880305937	21.129008886410816
3	15.630217159849053	27.820258802628345	33.09270085868545	23.45682317883715
4	11.238306883140098	18.60275571298187	44.54672954969442	25.612207854183616
5	10.930696468946465	39.098481561138804	38.76055193334966	11.210270036565074
6	25.096696228591224	43.879736894935114	19.697688973913866	11.325877902559803
7	23.66441300301993	34.12592070085056	26.79770875779781	15.4119575383317
8	21.070768097081462	38.34777604313052	24.580240294569872	16.001215565218143
9	22.717084872097864	15.190174370133258	25.5863312835717	36.50640947419717
10-14	20.25542365066928	26.17316127471852	33.12549285874448	20.44592221586772
15-19	19.581731174319888	31.800683675341624	28.929908709419085	19.68767644091941
20-24	19.516618940551105	30.204969531981696	30.579313622707456	19.699097904759743
25-29	19.498978905783787	30.732574086408032	30.047126214173137	19.721320793635048
30-34	19.37168834391531	30.508410260221456	30.150343596619898	19.969557799243336
35-39	19.505263764534046	30.749760108131635	30.07030004827755	19.67467607905677
40-44	19.26224835596765	30.47429703444279	30.497367028647737	19.766087580941825
45-49	19.393707299283406	30.271363506669296	30.508034206553212	19.826894987494086
50-54	19.081898581721205	29.971372412617047	30.918455700615365	20.028273305046383
55-59	19.24661974330345	29.874870156668564	31.02311270346058	19.855397396567394
60-64	18.89774182434088	29.524791683594636	31.24698839575739	20.33047809630709
65-69	19.2458032817864	29.555415577462128	30.72733142263236	20.471449718119114
70-74	19.2825280888702	29.415167241869405	30.563147496785398	20.739157172474997
75-79	19.682937540635457	29.777505470680037	30.00304062262378	20.53651636606073
80-84	19.859928720359925	29.766359430096713	29.750077198876745	20.62363465066662
85-89	20.093266704510828	29.712271507990184	29.625886037629485	20.5685757498695
90-94	20.291900989169807	29.446107110250434	29.850070340054085	20.411921560525673
95-99	20.80509456732332	29.24962460287267	29.381659204913923	20.56362162489009
100-101	21.205921787621527	28.54139291441924	29.837098878327637	20.41558641963159
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.72349333257729
2	36.088561699285876
3	39.08704033868621
4	36.850514737323714
5	22.140966505511535
6	36.42257413115102
7	39.07637054135163
8	37.07198366229961
9	59.223494346295034
10-14	40.701345866537004
15-19	39.2694076152393
20-24	39.21571684531085
25-29	39.22029969941884
30-34	39.34124614315865
35-39	39.179939843590816
40-44	39.02833593690947
45-49	39.22060228677749
50-54	39.11017188676759
55-59	39.102017139870846
60-64	39.228219920647966
65-69	39.71725299990552
70-74	40.0216852613452
75-79	40.21945390669619
80-84	40.48356337102654
85-89	40.661842454380334
90-94	40.70382254969548
95-99	41.368716192213405
100-101	41.62150820725312
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	3.5
3	8.0
4	11.0
5	18.5
6	21.5
7	14.5
8	18.0
9	29.0
10	49.0
11	68.0
12	96.0
13	130.0
14	194.0
15	282.5
16	395.0
17	572.0
18	856.0
19	1270.0
20	1872.0
21	2777.0
22	3984.5
23	5534.0
24	7490.0
25	9851.5
26	12526.0
27	15508.5
28	18937.0
29	22706.0
30	26769.0
31	31198.5
32	35341.5
33	39946.5
34	45787.0
35	52168.5
36	59392.0
37	66069.5
38	70518.5
39	71708.0
40	69735.5
41	65940.0
42	60871.5
43	56294.5
44	52322.5
45	47899.5
46	43827.0
47	39919.0
48	34943.5
49	29723.5
50	25528.5
51	22323.0
52	19578.0
53	16846.0
54	14385.0
55	12076.5
56	10304.5
57	8976.5
58	7447.5
59	6004.5
60	4841.0
61	3926.5
62	3079.0
63	2106.0
64	1426.0
65	1176.0
66	885.0
67	583.5
68	423.0
69	464.0
70	507.5
71	416.0
72	321.0
73	269.0
74	211.5
75	151.0
76	98.5
77	66.5
78	41.5
79	23.5
80	19.0
81	19.5
82	15.5
83	11.5
84	11.5
85	9.5
86	4.5
87	5.0
88	7.5
89	6.5
90	7.0
91	5.0
92	2.0
93	3.0
94	4.0
95	5.0
96	5.5
97	3.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037788253521392874
2	0.034009428169253585
3	0.030545504929792577
4	0.028734817781892498
5	0.035032860035457976
6	0.0016532360915609383
7	6.29804225356548E-4
8	0.003542648767630582
9	5.510786971869795E-4
10-14	0.00749467028174292
15-19	0.02637305193680544
20-24	0.022153363626916573
25-29	0.0023617658450870546
30-34	0.0020153735211409533
35-39	0.006329532464833306
40-44	0.014800399295878877
45-49	0.0091951416902056
50-54	0.0049282180634149875
55-59	0.00404649214791582
60-64	0.011714358591631792
65-69	0.015430203521235423
70-74	0.019602656514222556
75-79	0.007982768556394246
80-84	0.011005828838105676
85-89	0.009053435739500376
90-94	0.009478553591616045
95-99	0.009006200422598636
100-101	0.00621931672539591
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1270236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	21.653934214403925
#Duplication Level	Relative count
1	100.0
2	6.179833800143515
3	2.257470891877503
4	1.5098030138191245
5	1.0427999351866855
6	0.8113238119487974
7	0.5757968565542464
8	0.45774403370292355
9	0.3842503645748941
10++	2.5190389111363163
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	318950	3.5032566	6.030282	80-84
GCTGC	197220	3.32071	8.157749	90-94
TCTCT	462420	3.3132584	4.8290815	80-84
CTGTC	293390	3.222513	5.5335793	90-94
GAAGA	401100	3.1640124	5.334482	85-89
CTCTT	421170	3.0177004	4.7722197	1
GACGC	175615	3.0072927	7.9625144	95-97
CGCTG	173420	2.9199753	7.782117	90-94
CTGCC	172965	2.8467932	7.73954	90-94
CGACG	164660	2.8196952	8.297515	95-97
GCCGA	164545	2.817726	7.9893966	90-94
ACACA	364475	2.7471895	7.233596	6
CCGAC	157970	2.6442735	7.637233	95-97
TGCCG	155705	2.6216974	7.7148433	95-97
GAGGA	217820	2.5898812	6.037451	90-94
TACAC	326145	2.4171164	8.459174	5
CTGAC	208450	2.3285484	5.649336	95-97
AGAGG	192540	2.2893019	5.789495	90-94
TGACG	197745	2.2598064	5.600141	95-97
CAAAG	286070	2.2058477	5.2253914	4
GACGA	188990	2.1965375	5.9698615	95-97
CTTTG	283525	2.0782242	8.595218	9
CGAAG	174275	2.0255122	5.590286	95-97
ACGCT	180985	2.0217433	5.111999	85-89
AGAGA	237105	1.8703644	5.0573144	8
GAGAG	151530	1.8016928	6.1408653	7
GCTTT	235545	1.7265332	5.2376323	1
TATAC	335780	1.6607081	5.568431	5
TTGAG	193940	1.4790565	5.0002723	9
GACTC	125995	1.407462	5.4335313	7
GAGTC	122960	1.4051723	7.049404	9
GTGTA	179990	1.3726687	10.003576	1
AAGAC	177875	1.3715703	5.9659977	5
CCATG	118795	1.3270326	5.3360415	9
GTCTT	178110	1.3055375	6.4892592	1
GACTT	167650	1.2497945	6.2615347	7
GTGTT	165650	1.2421522	6.0384107	1
TGAGT	160490	1.2239547	5.033597	8
TATGA	241725	1.2230449	5.6131444	4
GATTG	158305	1.2072911	5.706649	7
TCATA	241690	1.1953558	5.7003846	2
GTTCT	162395	1.1903473	5.226965	1
ACATG	145305	1.1016654	5.0887156	8
TGGAC	94740	1.0826775	7.117869	5
GTCCA	94340	1.0538512	7.505237	1
ACACT	138625	1.0273737	5.035152	6
GGACT	87595	1.0010253	6.9827075	6
AGACT	131445	0.99658245	5.5370913	6
GTATG	122285	0.9325896	5.156966	3
GTATA	142650	0.7217596	7.031964	1
>>END_MODULE