##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-171_CGAGGCTG-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1270236	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44267207038692	34.0	31.0	34.0	31.0	34.0
2	32.63067965322979	34.0	31.0	34.0	31.0	34.0
3	32.74025614137845	34.0	31.0	34.0	31.0	34.0
4	36.25905894652647	37.0	37.0	37.0	35.0	37.0
5	35.98335427432383	37.0	35.0	37.0	35.0	37.0
6	36.071942536662476	37.0	36.0	37.0	35.0	37.0
7	36.07374456400228	37.0	36.0	37.0	35.0	37.0
8	36.09907528994612	37.0	36.0	37.0	35.0	37.0
9	37.96626926020047	39.0	38.0	39.0	35.0	39.0
10-14	38.15062712755739	39.2	38.2	39.4	35.2	39.4
15-19	39.28168796979459	40.0	39.0	41.0	36.0	41.0
20-24	39.21780960388464	40.0	39.0	41.0	36.0	41.0
25-29	39.02525011100299	40.0	38.8	41.0	36.0	41.0
30-34	38.82774460808857	40.0	38.0	41.0	35.0	41.0
35-39	38.60439808035672	40.0	38.0	41.0	34.8	41.0
40-44	38.36877068513253	40.0	38.0	41.0	34.0	41.0
45-49	38.20003936276409	40.0	38.0	41.0	33.4	41.0
50-54	38.151562701734164	40.0	37.6	41.0	33.4	41.0
55-59	37.777941421909006	39.8	36.6	41.0	33.0	41.0
60-64	37.22920512408719	39.0	35.6	40.8	32.0	41.0
65-69	36.49921353197359	37.8	35.0	40.0	31.0	41.0
70-74	35.92432099231954	36.6	35.0	39.2	31.0	41.0
75-79	34.72444821277306	35.2	34.0	37.4	30.4	39.2
80-84	34.50367002667221	35.0	34.0	36.6	31.0	38.4
85-89	33.86328776699762	35.0	34.0	35.6	30.2	36.8
90-94	33.43804222207527	35.0	34.0	35.0	29.8	36.0
95-99	33.15630670206166	35.0	34.0	35.0	29.0	35.6
100-101	32.80000291284455	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	9.0
15	51.0
16	100.0
17	174.0
18	373.0
19	691.0
20	945.0
21	1432.0
22	2015.0
23	2862.0
24	3809.0
25	4916.0
26	6277.0
27	8200.0
28	10314.0
29	13099.0
30	16693.0
31	21173.0
32	27270.0
33	36975.0
34	53023.0
35	84354.0
36	157743.0
37	311826.0
38	416962.0
39	88862.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04371156226087	23.65339984065953	11.018582373669144	24.284306223410454
2	15.146005947792649	23.75526652079326	39.74556927241505	21.35315825899905
3	15.728337096413581	27.52543621815159	33.32553950604454	23.42068717939029
4	11.505578490926096	18.593867596257706	44.14313560629679	25.75741830651942
5	10.74776655676583	39.26254648742438	38.827273042174845	11.162413913634946
6	25.186894403874554	43.998359359992946	19.538967561933372	11.275778674199126
7	23.499727609672533	34.274024669431505	26.81525322853391	15.410994492362049
8	20.977755314760408	38.44970540907359	24.51284643168671	16.059692844479294
9	22.582024127799873	15.061059519648317	25.547142420778503	36.80977393177331
10-14	20.19464099584644	26.181874864198463	33.092291511183745	20.531192628771347
15-19	19.513332238511417	31.866954259484448	28.92222444930484	19.6974890526993
20-24	19.42793163231293	30.264745055564134	30.58405836190699	19.72326495021594
25-29	19.445443992989706	30.768576629398424	30.022272128784337	19.763707248827536
30-34	19.33713645429854	30.514857381389188	30.162426510636774	19.985579653675494
35-39	19.469909343925234	30.812401482618174	30.025680085954175	19.692009087502417
40-44	19.224382657170633	30.54539714775538	30.44547674696478	19.784743448109207
45-49	19.35429192905869	30.291533974122274	30.517557633827806	19.836616462991223
50-54	19.046339296403882	29.93330140706621	30.92084701226851	20.0995122842614
55-59	19.131116205843647	29.881666917377913	30.96645641050278	20.02076046627566
60-64	18.731759664089534	29.57403745060163	31.120017668885463	20.57418521642337
65-69	19.0359781358789	29.665564009262326	30.471811351070365	20.826646503788414
70-74	18.93393398121847	29.46188898616263	30.292650513626374	21.311526518992522
75-79	19.308522062032797	29.654979017781507	29.68015579053177	21.356343129653922
80-84	19.362756984065303	29.487219051853735	29.36813866794979	21.781885296131172
85-89	19.499784127735918	29.279364532517672	29.26624844236588	21.95460289738053
90-94	19.490886394408186	28.788966901112722	29.564277997839394	22.155868706639694
95-99	19.740162118406804	28.536772813636574	29.278793368704985	22.444271699251633
100-101	19.586013774611086	27.73965929939763	30.107251765519074	22.56707516047221
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.32801778567132
2	36.4991642067917
3	39.14902427580387
4	37.26299679744551
5	21.910180470400775
6	36.462673078073685
7	38.91072210203458
8	37.0374481592397
9	59.39179805957318
10-14	40.725833624617785
15-19	39.21082129121072
20-24	39.15119658252887
25-29	39.20915124181724
30-34	39.32271610797404
35-39	39.16191843142765
40-44	39.00912610527984
45-49	39.19090839204992
50-54	39.14585158066529
55-59	39.15187667211931
60-64	39.3059448805129
65-69	39.86262463966731
70-74	40.245460500210996
75-79	40.66486519168672
80-84	41.14464228019648
85-89	41.454387025116446
90-94	41.64675510104788
95-99	42.18443381765844
100-101	42.1530889350833
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.5
7	2.5
8	3.5
9	9.5
10	19.0
11	34.0
12	62.0
13	98.0
14	148.5
15	244.5
16	384.5
17	554.0
18	830.0
19	1266.0
20	1885.0
21	2782.5
22	4026.5
23	5601.5
24	7507.0
25	9790.5
26	12398.0
27	15359.5
28	18980.5
29	22706.0
30	26336.0
31	30565.5
32	34983.5
33	39768.5
34	45062.5
35	51135.0
36	58476.5
37	64894.5
38	69124.5
39	70026.5
40	67646.5
41	63876.5
42	58917.5
43	54147.0
44	50356.0
45	46923.0
46	43859.5
47	40619.0
48	36710.0
49	32280.0
50	27918.5
51	24679.0
52	21765.5
53	18757.0
54	16074.0
55	13523.0
56	11641.5
57	9911.0
58	7948.5
59	6306.0
60	5030.5
61	4058.5
62	3193.0
63	2171.5
64	1418.5
65	1119.5
66	857.0
67	598.0
68	441.5
69	440.0
70	455.5
71	378.5
72	333.5
73	282.5
74	199.0
75	141.0
76	89.5
77	53.5
78	21.0
79	8.5
80	8.5
81	4.0
82	2.0
83	1.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014564222711370171
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011179025000078726
20-24	8.030003873295985E-4
25-29	0.017177910246599842
30-34	0.006786140528216803
35-39	0.04221262820452262
40-44	0.04923494531724813
45-49	0.04994347507077425
50-54	0.050699240141202105
55-59	0.04054364700732777
60-64	0.051927358380647375
65-69	0.045345904225671446
70-74	0.004093727464817561
75-79	0.0013855692957844053
80-84	1.2596084507130958E-4
85-89	0.003180511338050567
90-94	0.0028498641197383795
95-99	0.002251550105649659
100-101	3.936276408478425E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1270236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	21.995937736079515
#Duplication Level	Relative count
1	100.0
2	6.175660393771566
3	2.2634615942239105
4	1.5176733261808797
5	1.1192623307333198
6	0.8072607069330472
7	0.6124046742250703
8	0.47960100907588366
9	0.3821729927218952
10++	2.6282367268825935
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	290755	4.898611	14.677623	95-97
GTCTC	318835	3.4172206	5.83509	80-84
CTCCG	214435	3.352757	7.9452972	95-97
GAGCC	197530	3.327965	7.9653187	90-94
CGAGC	189525	3.1930976	7.9376736	85-89
TCTCT	458935	3.1792595	4.66837	80-84
CTGTC	294060	3.151686	5.4122443	90-94
TCTCC	291290	2.943742	5.9946175	95-97
GCCCA	184220	2.9265075	7.550581	90-94
AGCCC	183415	2.9137194	7.4165487	90-94
AGAAG	335935	2.7454605	5.226703	5
CCCAC	178400	2.6722364	7.0729837	90-94
ACACA	365290	2.6541903	6.9192476	6
ATCTC	371180	2.6125576	6.3764386	95-97
CCACG	162220	2.577017	7.2979207	90-94
TCCGA	229680	2.5011325	5.674528	95-97
CGAGG	138935	2.482505	7.8931384	90-94
GGCTG	136855	2.4067595	8.353969	95-97
GAGGC	134240	2.3986144	7.82729	90-94
TACAC	328050	2.3459992	8.571551	5
GAGAC	197830	2.321373	5.9799557	95-97
CGAGA	194035	2.2768419	6.0709925	95-97
GACCG	133985	2.2573657	7.218994	95-97
ACGAG	180625	2.1194866	5.7637444	95-97
CTTTG	283730	2.0845525	8.948502	9
AGAGA	236485	1.932696	5.1514134	6
CACGA	171875	1.9016591	5.227211	95-97
GAGAG	150155	1.8686391	6.4021244	7
AGGCT	161495	1.8651141	5.478676	95-97
AGACC	167915	1.8578452	5.3470273	95-97
GCTTT	236220	1.7354985	5.3334165	1
TGGAG	141260	1.7302082	5.0836544	5
GCTGA	146065	1.6869122	5.3069425	95-97
TATAC	337465	1.654311	5.6047378	5
AAGAG	197920	1.6175199	5.0523477	5
TTGAG	194015	1.5359682	5.454615	9
GAGTC	122690	1.4169533	7.4868793	9
GTCTT	188105	1.3819997	6.2419147	1
GACTC	125790	1.3698078	5.353953	7
AAGAC	176860	1.3628772	5.9520426	5
CCATG	121665	1.324888	5.533474	9
TGAGT	161605	1.2793864	5.1743994	8
GTGTT	163820	1.2764604	6.1362433	1
ATGAG	156335	1.2575049	5.0350385	7
TATGA	241720	1.2567064	5.7525444	4
GACTT	168295	1.2562768	6.32672	7
GATTG	156695	1.2405151	6.3029404	7
GTATG	154090	1.219892	5.1820765	3
GTTCT	163140	1.1985831	5.0519614	1
TCATA	243790	1.1951001	5.926343	2
AACAC	163835	1.190422	5.1295443	5
GTGTA	142715	1.129839	10.104362	1
ACATG	146305	1.1096342	5.0380716	8
TGGAC	94305	1.0891334	7.587675	5
GTCCA	95160	1.0362581	7.324299	1
GGACT	87745	1.0133716	7.106096	6
ACACT	139325	0.9963613	5.2323904	6
AGACT	131140	0.99461687	5.6485243	6
GCCGT	58320	0.9670673	5.1378627	95-97
GAGTA	120030	0.96547997	5.0551558	1
TGCCG	53210	0.8823329	5.2772317	95-97
GTATA	142015	0.73833835	6.8096046	1
>>END_MODULE