##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-170_CGAGGCTG-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2431957	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73419636942594	33.0	31.0	34.0	30.0	34.0
2	31.928693229362196	33.0	31.0	34.0	30.0	34.0
3	32.073914958200334	34.0	31.0	34.0	30.0	34.0
4	35.60714930403786	37.0	35.0	37.0	33.0	37.0
5	35.47913922820182	37.0	35.0	37.0	33.0	37.0
6	35.61854013043816	37.0	35.0	37.0	33.0	37.0
7	35.63647424687196	37.0	35.0	37.0	33.0	37.0
8	35.688480922976844	37.0	35.0	37.0	33.0	37.0
9	37.50189826547098	39.0	38.0	39.0	35.0	39.0
10-14	37.630631380406804	39.2	37.2	39.4	34.6	39.4
15-19	38.69162300155801	40.0	38.0	41.0	34.6	41.0
20-24	38.59099424866476	40.0	38.0	41.0	34.0	41.0
25-29	38.409750254630325	40.0	38.0	41.0	34.0	41.0
30-34	38.14966917589415	40.0	38.0	41.0	33.2	41.0
35-39	37.842165383680715	40.0	37.8	41.0	32.8	41.0
40-44	37.80101062642144	40.0	37.8	41.0	32.8	41.0
45-49	37.64090384821771	40.0	37.2	41.0	32.2	41.0
50-54	36.932045426790026	39.2	36.4	40.2	31.4	40.6
55-59	37.55086549638829	40.0	36.6	41.0	32.0	41.0
60-64	37.093334298262675	39.0	35.4	41.0	31.6	41.0
65-69	36.41318649959682	38.0	35.0	40.2	31.0	41.0
70-74	35.58277543558542	36.6	34.8	39.2	30.0	41.0
75-79	34.64086782784399	35.4	34.0	37.6	29.2	39.4
80-84	33.73402967239963	35.0	34.0	36.4	28.8	38.0
85-89	32.96692548429105	35.0	33.0	35.4	27.2	36.6
90-94	32.38004627548925	35.0	33.0	35.0	25.6	36.0
95-99	31.87203762237573	35.0	32.0	35.0	24.6	35.2
100-101	30.97057184810422	34.5	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	81.0
7	254.0
8	518.0
9	718.0
10	847.0
11	1065.0
12	1448.0
13	2046.0
14	2541.0
15	3029.0
16	3475.0
17	4078.0
18	4826.0
19	5483.0
20	6350.0
21	7366.0
22	8547.0
23	9603.0
24	11710.0
25	13525.0
26	16024.0
27	19301.0
28	23163.0
29	27992.0
30	35303.0
31	45051.0
32	59744.0
33	81708.0
34	117194.0
35	183289.0
36	319805.0
37	586292.0
38	694138.0
39	135358.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.640228419871036	23.305926403982994	10.747728326293418	24.306116849852557
2	15.180395918407639	23.496056281486403	39.928669204773925	21.39487859533203
3	15.761606782232013	27.55573780875534	32.7212587206648	23.96139668834785
4	11.188229332727904	18.308153719853973	44.28913862853777	26.214478318880353
5	10.827761914281178	39.18663451737814	38.9346969633401	11.050906605000579
6	25.610800150664588	43.74071710421351	19.414801315189983	11.233681429931922
7	24.05495045922261	33.827699413142454	26.605017566251743	15.512332561383193
8	21.374803237675728	38.44567221576171	24.12100492296457	16.05851962359799
9	23.08498879908419	14.747132335284036	24.799255246008954	37.368623619622824
10-14	20.524535537250983	25.814261944382327	32.842724297636124	20.818478220730558
15-19	19.91845967020125	31.542925026542477	28.49612759933039	20.042487703925886
20-24	19.838796841082704	29.89953386146465	30.230173895090452	20.031495402362197
25-29	19.863219412287076	30.410978303831236	29.674334597039316	20.051467686842376
30-34	19.677013247575477	30.1735974843719	29.81325395731472	20.3361353107379
35-39	19.865625661546023	30.489296092825917	29.662559920546215	19.982518325081845
40-44	19.532351570747483	30.22658622075141	30.186004105094028	20.05505810340708
45-49	19.74524105551028	30.04490390406617	30.132389082290594	20.07746595813295
50-54	19.457383818068095	29.657436371494335	30.582935927175264	20.302243883262307
55-59	19.59974195683501	29.5974918099093	30.71337059344171	20.089395639813983
60-64	19.225034671497127	29.19873845263547	31.021963248130795	20.554263627736606
65-69	19.573970243848837	29.326492528720127	30.452703498529036	20.646833728902
70-74	19.422169828780152	29.170651147357706	30.474421484659864	20.932757539202274
75-79	19.790833847708914	29.52599176478184	29.926238336079642	20.75693605142961
80-84	19.958178876269496	29.52588771807983	29.600921959700617	20.915011445950054
85-89	20.250143725310707	29.549882757979734	29.38333411467265	20.81663940203691
90-94	20.31346241644647	29.197567829579302	29.783716328044125	20.705253425930106
95-99	20.856514846517786	29.047153963355083	29.206054163419644	20.890277026707487
100-101	21.111479151927043	28.313811749384353	29.825739869984957	20.748969228703647
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.9463452697236
2	36.57527451373967
3	39.72300347057987
4	37.40270765160825
5	21.878668519281756
6	36.84448158059651
7	39.56728302060581
8	37.43332286127372
9	60.45361241870702
10-14	41.343013757981545
15-19	39.96094737412713
20-24	39.8702922434449
25-29	39.91468709912945
30-34	40.013148558313375
35-39	39.84814398662787
40-44	39.587409674154564
45-49	39.82270701364323
50-54	39.75962770133041
55-59	39.68913759664899
60-64	39.77929829923374
65-69	40.220803972750836
70-74	40.35492736798243
75-79	40.54776989913852
80-84	40.87319032221956
85-89	41.06678312734762
90-94	41.01871584237658
95-99	41.74679187322528
100-101	41.860448380630686
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.5
2	5.5
3	9.0
4	14.5
5	20.5
6	30.0
7	39.5
8	48.5
9	61.0
10	73.0
11	95.5
12	130.0
13	183.0
14	251.5
15	356.5
16	557.5
17	807.5
18	1168.5
19	1742.5
20	2592.0
21	3844.5
22	5639.0
23	8111.5
24	11164.5
25	14821.0
26	19486.5
27	25050.5
28	31239.5
29	38131.0
30	45895.5
31	53933.5
32	62330.5
33	72067.0
34	83459.0
35	96810.5
36	112643.5
37	127927.5
38	139252.0
39	143081.5
40	139039.0
41	131304.5
42	120975.0
43	110630.0
44	102743.5
45	94298.5
46	85561.0
47	77165.5
48	67936.5
49	58152.5
50	49806.0
51	44384.0
52	39285.0
53	34226.0
54	29674.0
55	25611.5
56	22273.5
57	18975.0
58	15592.0
59	12865.5
60	10458.0
61	8472.0
62	6799.5
63	4814.0
64	3372.5
65	2702.0
66	2050.5
67	1515.5
68	1212.5
69	1233.0
70	1234.0
71	983.0
72	841.5
73	717.0
74	518.5
75	365.5
76	275.0
77	196.5
78	134.0
79	88.5
80	61.5
81	56.0
82	42.0
83	26.5
84	26.5
85	22.0
86	19.5
87	21.5
88	19.5
89	18.5
90	15.5
91	10.0
92	8.5
93	10.0
94	10.5
95	7.5
96	6.0
97	5.5
98	4.5
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03371770142317484
2	0.03079824190970482
3	0.027261995175079167
4	0.025699467548151552
5	0.03285419931355694
6	0.002672744625007761
7	8.635021096178921E-4
8	0.0036596041788567804
9	5.345489250015523E-4
10-14	0.007829085793868889
15-19	0.023487257381606664
20-24	0.020189501705827857
25-29	0.0025576110103920423
30-34	0.0022780008034681536
35-39	0.006307677315018316
40-44	0.014144986938502614
45-49	0.009490299376181404
50-54	0.006003395619248202
55-59	0.00476159734732152
60-64	0.011373556358109951
65-69	0.014482163952734363
70-74	0.018084201324283285
75-79	0.00847054450387075
80-84	0.01098703636618575
85-89	0.009662999798104983
90-94	0.010378472974645522
95-99	0.00995083383464428
100-101	0.0066407424144423605
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2431957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.698442867356015
#Duplication Level	Relative count
1	100.0
2	8.240918383393616
3	2.9073753734863974
4	1.9242019185406511
5	1.56817109608429
6	1.248623997483881
7	1.0341248623997483
8	0.8900770561409027
9	0.7485453687686743
10++	7.243906274571473
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	587885	3.2999358	5.9117684	90-94
TCTCT	845070	3.1547356	4.7541723	95-97
CTGTC	544400	3.0558445	5.5442567	90-94
GCTGC	359520	3.0344431	7.6283946	90-94
CTCTT	781125	2.9160223	5.036152	1
GACGC	315705	2.7116976	7.469733	95-97
ACACA	681170	2.680015	7.7102847	6
AGAAG	645870	2.6689363	5.0855246	5
CGCTG	312975	2.641591	7.227245	90-94
CTGCC	314695	2.591732	7.1703043	90-94
GCCGA	296850	2.5497456	7.4572353	95-97
CGACG	293840	2.5238917	7.6822023	95-97
GAGAG	404245	2.4681797	5.91906	7
TACAC	616525	2.383574	9.532795	5
CCGAC	284030	2.3805008	7.1626716	95-97
TGCCG	280685	2.369055	7.1191797	90-94
CACAT	591415	2.286495	5.32856	7
CAAAG	560910	2.2616773	5.310427	4
CTGAC	379045	2.1652484	5.4785023	95-97
CTTTG	550625	2.106599	8.939167	9
TGACG	357735	2.0942767	5.4498935	95-97
GACGA	338045	2.0139606	5.552896	95-97
AGAGG	324170	1.9792693	5.5328827	95-97
ATACA	715660	1.9191164	5.187504	6
GCTTT	463760	1.7742682	5.1742363	1
GCCAA	286995	1.6683805	5.3664503	1
TATAC	627175	1.6526445	5.9936743	5
TGGCT	279765	1.6093932	5.361941	6
TTGAG	373615	1.4907669	5.5845222	9
GACTC	257265	1.469595	5.7705083	7
GAGTC	246660	1.4440137	7.3588824	9
AAGAC	350745	1.4142588	5.879611	5
GTGTA	348655	1.3911736	10.744451	1
CCATG	243380	1.3902786	5.850798	9
GTCTT	348095	1.3317531	6.707206	1
GACTT	333995	1.3003783	6.740713	7
TATGA	476535	1.2868894	6.407171	4
TCATA	480735	1.2667663	6.616097	2
TGAGT	313320	1.2501829	5.3369694	8
ACTCA	318800	1.2325264	5.098027	8
GTGTT	309480	1.2134285	5.8809657	1
GATTG	303630	1.2115186	6.2521625	7
AACAC	307395	1.2094238	5.1798086	5
GTTCT	308665	1.1809006	5.1129913	1
ACACC	203920	1.1567112	5.22384	6
TGGAC	190555	1.11556	7.6193066	5
ACATG	280390	1.1109542	5.5820637	8
GTCCA	189350	1.0816388	8.01665	1
GGACT	175985	1.0302633	7.2253723	6
ACACT	263710	1.0195405	5.2890882	6
AGACT	257265	1.0193288	5.772094	6
GAGTA	240490	0.9765315	5.505523	1
GTATG	237095	0.94603634	5.585901	3
CATAC	232700	0.89965135	5.1816754	3
GTATA	279415	0.7545641	7.3111544	1
>>END_MODULE