##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-170_CGAGGCTG-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2431957	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41491605320324	34.0	31.0	34.0	31.0	34.0
2	32.601821496021515	34.0	31.0	34.0	31.0	34.0
3	32.709982947889294	34.0	31.0	34.0	31.0	34.0
4	36.23497002619701	37.0	35.0	37.0	35.0	37.0
5	35.95729283042422	37.0	35.0	37.0	35.0	37.0
6	36.05184466666146	37.0	36.0	37.0	35.0	37.0
7	36.047819102064715	37.0	36.0	37.0	35.0	37.0
8	36.07130553706336	37.0	36.0	37.0	35.0	37.0
9	37.93463042315304	39.0	38.0	39.0	35.0	39.0
10-14	38.10919748992273	39.2	38.2	39.4	35.2	39.4
15-19	39.230880809159046	40.0	39.0	41.0	36.0	41.0
20-24	39.156312056504284	40.0	39.0	41.0	36.0	41.0
25-29	38.96216980810105	40.0	38.6	41.0	35.6	41.0
30-34	38.75792071981536	40.0	38.0	41.0	35.0	41.0
35-39	38.522877994964546	40.0	38.0	41.0	34.2	41.0
40-44	38.280822810600675	40.0	38.0	41.0	33.8	41.0
45-49	38.09766348664881	40.0	38.0	41.0	33.2	41.0
50-54	38.03911812585502	40.0	37.2	41.0	33.0	41.0
55-59	37.64628239726278	39.6	36.4	41.0	33.0	41.0
60-64	37.0814511111833	38.8	35.4	40.4	31.8	41.0
65-69	36.3319499481282	37.6	35.0	40.0	31.0	41.0
70-74	35.75434935732828	36.6	34.8	39.0	31.0	40.8
75-79	34.57492990213232	35.2	33.6	37.4	30.2	39.2
80-84	34.389313626844555	35.0	34.0	36.4	31.0	38.2
85-89	33.77223676240986	35.0	34.0	35.6	30.0	36.6
90-94	33.36180138053428	35.0	34.0	35.0	29.4	36.0
95-99	33.08924993328418	35.0	33.6	35.0	29.0	35.4
100-101	32.73517356597999	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	8.0
14	24.0
15	84.0
16	208.0
17	446.0
18	767.0
19	1316.0
20	1918.0
21	2852.0
22	3950.0
23	5526.0
24	7463.0
25	9907.0
26	12615.0
27	16428.0
28	20643.0
29	26227.0
30	32924.0
31	41977.0
32	54805.0
33	73537.0
34	107222.0
35	171118.0
36	317044.0
37	614159.0
38	763139.0
39	145568.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.128852195988664	23.393711319731395	11.11565706137074	24.361779422909205
2	15.395671677882818	23.462665918740324	39.68038486403259	21.46127753934427
3	15.933382045817421	27.288722621329242	32.94885559243029	23.829039740423042
4	11.358342273321446	18.335357080737857	43.97499626843731	26.331304377503383
5	10.651380760432854	39.34748846299503	39.002581048924796	10.998549727647323
6	25.63092192830712	43.96952742174307	19.218308547396195	11.181242102553622
7	23.98866427325812	33.83838612278095	26.59302775501376	15.579921848947166
8	21.28869877222336	38.58312461939089	24.01247225999473	16.115704348391027
9	22.98071059644558	14.589608286659676	24.77473902704694	37.6549420898478
10-14	20.47229453481291	25.80256970003993	32.835597010966886	20.889538754180276
15-19	19.847732409315206	31.62221169925506	28.468534363582627	20.06152152784711
20-24	19.785753184655494	29.997819027651474	30.177272261502203	20.03915552619083
25-29	19.80469541677524	30.502862047908952	29.576307349531096	20.11613518578471
30-34	19.656308256854174	30.25572792842687	29.723791730175986	20.364172084542968
35-39	19.832500134103885	30.52168221229616	29.644091580283472	20.001726073316487
40-44	19.51570181784193	30.27210033576463	30.15142978069653	20.060768065696912
45-49	19.729692904204242	30.0592114422138	30.075914130737786	20.135181522844174
50-54	19.411763931486828	29.68752211262224	30.537288586875494	20.363425369015438
55-59	19.543810121572037	29.625241806748598	30.646101708692274	20.18484636298709
60-64	19.067161514189976	29.274354367479745	30.91503292711866	20.743451191211623
65-69	19.37034635845607	29.400030935146443	30.268206074148914	20.961416632248575
70-74	19.192289611789644	29.26155026891386	30.128278934845177	21.41788118445132
75-79	19.538675462572936	29.454654578984368	29.541104819600918	21.465565138841782
80-84	19.57782883872708	29.312387744494956	29.15677612058133	21.953007296196635
85-89	19.766756007918175	29.172366660443753	28.99868167511968	22.062195656518387
90-94	19.69882671454166	28.61127296517234	29.39464753531103	22.295252784974974
95-99	19.917999877297525	28.405935312768772	29.041414058841593	22.63465075109211
100-101	19.791538169276087	27.569228226168562	29.908148009256745	22.7310855952986
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.49063161889786
2	36.85694921722709
3	39.762421786240466
4	37.689646650824834
5	21.649930488080177
6	36.81216403086074
7	39.56858612220528
8	37.40440312061439
9	60.63565268629338
10-14	41.36183328899318
15-19	39.90925393716231
20-24	39.82490871084632
25-29	39.92083060255995
30-34	40.02048034139714
35-39	39.83422620742037
40-44	39.57646988353885
45-49	39.86487442704841
50-54	39.77518930050227
55-59	39.728656484559124
60-64	39.810612705401596
65-69	40.33176299070465
70-74	40.610170796240965
75-79	41.004240601414715
80-84	41.53083613492372
85-89	41.828951664436566
90-94	41.99407949951663
95-99	42.552650628389635
100-101	42.52262376457469
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	1.5
6	3.5
7	6.5
8	11.0
9	14.0
10	23.5
11	39.0
12	69.0
13	112.0
14	191.0
15	308.0
16	471.5
17	722.5
18	1116.5
19	1689.5
20	2549.5
21	3901.5
22	5739.5
23	8144.0
24	11192.0
25	14976.0
26	19577.0
27	24836.0
28	31038.5
29	37976.0
30	45418.5
31	53413.5
32	61727.0
33	71067.5
34	82126.0
35	95131.0
36	110746.0
37	126223.5
38	136474.5
39	139482.5
40	134938.0
41	126468.5
42	116504.0
43	106326.5
44	98971.0
45	92645.0
46	86153.5
47	78987.0
48	71047.0
49	62306.5
50	54291.5
51	48819.0
52	43364.5
53	37776.0
54	32886.5
55	28471.0
56	24779.5
57	20867.5
58	16945.0
59	13917.5
60	11112.5
61	8816.0
62	6910.5
63	4825.5
64	3373.5
65	2628.5
66	2023.5
67	1499.0
68	1159.5
69	1193.0
70	1257.5
71	1017.0
72	845.0
73	738.0
74	544.0
75	392.0
76	265.0
77	161.5
78	80.5
79	45.5
80	29.5
81	17.0
82	10.5
83	8.5
84	8.5
85	5.5
86	2.5
87	1.5
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014186106086579655
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011431123165417808
20-24	5.838919026940032E-4
25-29	0.016916417518895277
30-34	0.006644854329250065
35-39	0.04135763913588933
40-44	0.049614364069759456
45-49	0.04977884066206763
50-54	0.050107793846683964
55-59	0.04057637532242552
60-64	0.05162097849591913
65-69	0.04379189270205024
70-74	0.004120138637319657
75-79	0.0015543037973122056
80-84	2.055957403852124E-4
85-89	0.003412889290394526
90-94	0.0027961020692388885
95-99	0.0023931344180838725
100-101	4.111914807704248E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2431957.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.321453829165957
#Duplication Level	Relative count
1	100.0
2	8.509643631033981
3	2.847287590576849
4	2.036123342451561
5	1.5364107877264026
6	1.2742354271563134
7	1.0265902672925182
8	0.8560183459577614
9	0.7650466545792244
10++	7.498215312304553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	2479	0.10193436808298832	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	520290	4.390524	13.585603	90-94
GTCTC	585585	3.2220805	5.740527	90-94
CTCCG	396040	3.1158435	7.492203	95-97
GAGCC	365120	3.081105	7.513368	90-94
TCTCT	843415	3.0694442	4.607011	80-84
CTGTC	545520	3.0016298	5.4342375	90-94
CGAGC	346600	2.9248219	7.38413	95-97
TCTCC	533410	2.7756824	5.70228	95-97
GCCCA	345070	2.7538497	7.1032147	90-94
AGCCC	344695	2.750857	7.0165334	90-94
AGAAG	647170	2.748538	5.275586	5
ACACA	683965	2.5980153	7.5085626	6
ATCTC	672645	2.4831378	6.146169	95-97
CCCAC	325180	2.4542515	6.6576276	90-94
TCCGA	423775	2.3652563	5.463098	95-97
CCACG	292395	2.3334742	6.811762	95-97
TACAC	621570	2.327563	9.624484	5
CGAGG	255340	2.2783873	7.366566	90-94
CAAAG	564460	2.2671423	5.333913	4
CACAT	592160	2.2174327	5.366907	7
GGCTG	251785	2.2148387	7.71298	95-97
GAGGC	242690	2.1655118	7.3129153	90-94
GAGAC	358350	2.1452827	5.7271123	95-97
CTTTG	552650	2.1267009	9.064044	9
CGAGA	353950	2.1189418	5.5742345	95-97
GACCG	242485	2.0462363	6.689907	95-97
ACGAG	329405	1.9720019	5.552948	95-97
GAGAG	285525	1.80742	5.809969	7
CACGA	317925	1.799965	5.0255094	95-97
GCTTT	458410	1.7640479	5.344403	1
AGACC	307020	1.738225	5.058451	95-97
AGGCT	294225	1.736442	5.095594	95-97
TATAC	626725	1.6413387	6.0886087	5
TGGCT	278640	1.6211667	5.2638574	6
GCTGA	263695	1.5562615	5.116579	95-97
TTGAG	370990	1.5312734	5.8776364	9
GAGTC	247090	1.4582629	7.345389	9
GACTC	257180	1.4354236	5.3879356	7
AAGAC	352780	1.4169338	6.1615543	5
GTCTT	363115	1.3973348	6.3222194	1
CATGA	345610	1.3684705	5.0414114	9
CCATG	244290	1.3634794	5.7776203	9
TATGA	478095	1.3239548	6.5575695	4
GACTT	334710	1.3065361	6.947768	7
TGAGT	313495	1.2939608	5.389334	8
ATGAG	308650	1.29227	5.0729604	7
TCATA	483930	1.2673709	6.4882407	2
GTGTT	308025	1.2533722	5.891878	1
GATTG	300115	1.2387342	6.5200334	7
ACTCA	320395	1.1997675	5.1671906	8
GTGTA	288445	1.1905662	10.864262	1
GTTCT	307305	1.1825672	5.0831532	1
GTATG	285315	1.1776468	5.687518	3
ACATG	284175	1.1252136	5.6999044	8
TGGAC	188465	1.1122729	7.6487045	5
ACACC	206885	1.1077214	5.2439814	6
GTCCA	191445	1.0685306	7.5295653	1
GGACT	176050	1.0390028	7.322498	6
AGACT	257305	1.0188198	5.6883855	6
GAGTA	239840	1.0041732	5.6035843	1
ACACT	265355	0.9936619	5.1599283	6
TGTAT	362335	0.98917425	5.083509	2
CATAC	235525	0.881959	5.2162123	3
GTATA	278820	0.7721166	7.133241	1
ATACT	288235	0.7548626	5.0448475	6
>>END_MODULE