##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-168_GCTACGCT-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1140236	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52430637166341	31.0	31.0	34.0	30.0	34.0
2	31.71155006507425	33.0	31.0	34.0	30.0	34.0
3	31.85400829302004	34.0	31.0	34.0	30.0	34.0
4	35.381254406982414	37.0	35.0	37.0	33.0	37.0
5	35.26212643698322	37.0	35.0	37.0	32.0	37.0
6	35.402885893797425	37.0	35.0	37.0	33.0	37.0
7	35.40846631749918	37.0	35.0	37.0	33.0	37.0
8	35.45822794579368	37.0	35.0	37.0	33.0	37.0
9	37.23054613255501	39.0	37.0	39.0	34.0	39.0
10-14	37.32954949677084	39.2	37.2	39.4	33.2	39.4
15-19	38.352585955889836	40.0	38.0	41.0	33.2	41.0
20-24	38.234652650854734	40.0	38.0	41.0	33.2	41.0
25-29	38.04691502460894	40.0	38.0	41.0	33.0	41.0
30-34	37.7606770879011	40.0	37.8	41.0	32.0	41.0
35-39	37.42683093675344	40.0	37.0	41.0	31.2	41.0
40-44	37.3601801732273	40.0	37.0	41.0	31.0	41.0
45-49	37.1796066779158	40.0	36.6	41.0	30.6	41.0
50-54	36.493736033593045	38.6	35.4	40.2	30.2	40.6
55-59	37.13075082702178	39.8	36.0	41.0	31.0	41.0
60-64	36.673914873762975	39.0	35.0	41.0	30.2	41.0
65-69	35.99783395718079	37.6	35.0	40.0	29.2	41.0
70-74	35.16316464310897	36.4	34.0	39.2	28.8	40.8
75-79	34.21455733725299	35.4	34.0	37.6	27.8	39.4
80-84	33.308089377988416	35.0	33.0	36.4	26.4	38.0
85-89	32.56494129285517	35.0	32.8	35.4	25.6	36.6
90-94	32.016068603341765	35.0	32.0	35.0	25.0	36.0
95-99	31.56103981982677	34.4	31.6	35.0	23.6	35.2
100-101	30.718801634047686	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	35.0
7	126.0
8	256.0
9	352.0
10	416.0
11	691.0
12	973.0
13	1454.0
14	1782.0
15	2221.0
16	2425.0
17	2819.0
18	3218.0
19	3633.0
20	4117.0
21	4705.0
22	5300.0
23	6067.0
24	6679.0
25	8062.0
26	9481.0
27	10990.0
28	12915.0
29	15446.0
30	19162.0
31	24062.0
32	31124.0
33	41244.0
34	58168.0
35	88394.0
36	147583.0
37	262057.0
38	306545.0
39	57687.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.39459145993208	22.973661792535182	10.300247318233382	24.33149942929936
2	15.088998005890211	23.506281928347153	40.22258932480127	21.182130740961366
3	15.85712481051036	27.821816574027284	32.19393913873449	24.127119476727866
4	10.844502304559818	18.37033320057478	44.640736055527	26.14442843933841
5	10.417123596530784	39.69929482196843	39.19352829397449	10.690053287526297
6	25.43903962056312	44.2889768254436	19.39378152911378	10.878202024879496
7	24.34405490144489	33.567936152952264	26.67631388541735	15.41169506018549
8	21.19176314887041	39.09995939283964	23.762906806128438	15.94537065216152
9	23.11202398816378	14.51778546239396	24.301434185589034	38.068756363853225
10-14	20.553452036700335	25.683588079845425	33.04291617002279	20.720043713431444
15-19	19.894749862055473	31.655062366715764	28.365394652928067	20.0847931183007
20-24	19.877245183316433	29.863455661247933	30.22736921883936	20.031929936596267
25-29	19.8471685040475	30.556511519807756	29.534787451434386	20.06153252471036
30-34	19.656077147947936	30.311700694925108	29.54301719368691	20.48920496344004
35-39	19.93967200600052	30.65159215244922	29.356834681790527	20.051901159759726
40-44	19.521356228108985	30.37024783181806	29.954259334172807	20.15413660590015
45-49	19.802175240873844	30.241994757838597	29.761299573280624	20.19453042800694
50-54	19.601797969609926	29.72926085907865	30.29284101124827	20.37610016006315
55-59	19.88076348137798	29.75087608390058	30.391472420854253	19.976888013867192
60-64	19.499287618597208	29.259368929364953	30.764193510926397	20.477149941111442
65-69	19.95208728702683	29.584223188475967	30.084577025405036	20.379112499092162
70-74	19.826502293893846	29.323982440409722	30.204540841415454	20.644974424280978
75-79	20.10944202808936	29.787492314587503	29.666788580770724	20.436277076552408
80-84	20.1549471048812	29.613197097952014	29.56809599675539	20.6637598004114
85-89	20.36208437525491	29.688778647178676	29.31922138088852	20.629915596677897
90-94	20.35313686395351	29.200626744782888	29.951271505320697	20.494964885942903
95-99	20.7391389541308	29.102727986051942	29.397698023306052	20.760435036511204
100-101	20.850567817035564	28.179959584477913	30.384526327976168	20.58494627051035
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.72609088923144
2	36.27112874685158
3	39.984244287238226
4	36.98893074389822
5	21.10717688405708
6	36.317241645442614
7	39.755749961630386
8	37.13713380103193
9	61.180780352017
10-14	41.27349575013178
15-19	39.97954298035617
20-24	39.909175119912696
25-29	39.90870102875786
30-34	40.14528211138798
35-39	39.99157316576025
40-44	39.675492834009134
45-49	39.99670566888078
50-54	39.97789812967308
55-59	39.857651495245165
60-64	39.97643755970865
65-69	40.331199786119
70-74	40.47147671817482
75-79	40.54571910464177
80-84	40.8187069052926
85-89	40.9919999719328
90-94	40.848101749896415
95-99	41.499573990642006
100-101	41.43551408754592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	4.0
2	4.5
3	5.5
4	7.5
5	11.5
6	13.0
7	19.0
8	24.5
9	29.0
10	40.0
11	53.0
12	80.0
13	123.5
14	180.0
15	240.5
16	334.5
17	492.5
18	724.5
19	1044.5
20	1450.0
21	2086.0
22	2990.0
23	4121.5
24	5538.0
25	7197.5
26	9329.5
27	11884.5
28	14846.5
29	18108.5
30	21667.5
31	25623.5
32	29690.5
33	34598.0
34	40474.0
35	47172.5
36	55686.0
37	63897.5
38	69152.0
39	69844.5
40	65957.5
41	60496.5
42	54377.0
43	48380.0
44	44009.0
45	39994.5
46	36007.5
47	32525.5
48	28722.0
49	24568.5
50	21116.0
51	18844.5
52	16739.0
53	14836.5
54	13122.5
55	11355.0
56	10133.5
57	8959.5
58	7568.0
59	6550.0
60	5610.0
61	4804.0
62	4204.5
63	3341.5
64	2610.0
65	2308.5
66	2039.0
67	1760.5
68	1590.5
69	1506.0
70	1389.5
71	1188.0
72	1031.0
73	852.5
74	679.5
75	554.5
76	428.5
77	331.0
78	243.0
79	171.5
80	130.0
81	97.5
82	65.0
83	43.0
84	35.5
85	29.5
86	22.0
87	18.0
88	15.0
89	14.0
90	12.5
91	9.0
92	11.0
93	9.0
94	4.5
95	6.0
96	6.0
97	3.5
98	3.5
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03586976731132853
2	0.033238733034213964
3	0.029116779333401156
4	0.0282397679076963
5	0.03350183646192542
6	0.0017540228514097082
7	9.647125682753395E-4
8	0.0037711491305308723
9	6.139079979933979E-4
10-14	0.007279194833350289
15-19	0.02499482563258834
20-24	0.021048274216916497
25-29	0.0022802297068326206
30-34	0.002350390620889009
35-39	0.006033838608849396
40-44	0.013716458698023919
45-49	0.008384229229738405
50-54	0.00534976969679961
55-59	0.004244735300411494
60-64	0.0102084129952045
65-69	0.014277746010475025
70-74	0.018364619254259643
75-79	0.007805401688773201
80-84	0.011471309448219492
85-89	0.010085631395605822
90-94	0.010769700307655608
95-99	0.010541677336972347
100-101	0.007016091405638833
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1140236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.13920817369093
#Duplication Level	Relative count
1	100.0
2	6.904076266790677
3	2.9154518950437316
4	1.8673201094635927
5	1.4273193940152749
6	1.0379962677446088
7	0.8036869436644071
8	0.6170741741043482
9	0.5145265818860411
10++	3.153934667644446
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1329	0.1165548184761751	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	136435	2.457991	5.1710663	90-94
CAAAG	287895	2.44289	5.075388	4
CTTTG	279705	2.3127484	10.822188	9
ACACA	274210	2.2923272	9.243104	6
GGCTT	168980	2.077853	5.0925655	7
TACAC	242395	2.0089529	11.635392	5
GCTTT	236155	1.9526541	5.475189	1
CACAT	228725	1.895657	6.792537	7
GCCAA	144950	1.786552	6.0089946	1
TTGGC	138540	1.7035495	5.392429	5
TGGCT	138400	1.7018278	6.0226917	6
CTCCA	140770	1.6946652	5.1158314	1
ATACA	288435	1.6457592	5.8403277	4
CACCA	134750	1.6362504	5.040737	7
GAGAG	127895	1.6240672	5.5484858	7
GACTC	132340	1.6171169	6.553973	7
TTGAG	186665	1.5802097	6.8557076	9
GAGTC	124450	1.5435535	8.817485	9
AAGAC	175255	1.4871	6.6519275	5
ATGGA	172920	1.4765366	5.3144956	4
CCATG	118565	1.4487945	6.198205	9
CATGA	172010	1.4470257	6.31914	9
GTGTA	169505	1.434942	12.870313	1
GACTT	170050	1.4182475	8.10536	7
GTCTT	170075	1.4062698	7.3604193	1
TATGA	243965	1.4007968	7.9828906	4
TCCAT	169890	1.3959398	5.3769007	2
TCATA	246715	1.3956184	7.9604945	2
ACTCA	164970	1.3672597	5.9686685	8
TGAGT	159590	1.3510066	6.236121	8
TATAC	235850	1.3341572	6.289587	5
ATGAG	154450	1.3188242	5.896689	7
AACAC	156195	1.3057512	5.446352	5
GTGTT	154670	1.2981076	6.388014	1
GATTG	151980	1.2865845	7.664705	7
ACACC	101565	1.2332896	5.4881587	6
GTTCT	148290	1.2261399	5.282655	1
ATGAT	212965	1.2228011	5.038747	5
ACCAT	146130	1.2111152	5.245194	8
TGGAC	95775	1.1878972	8.808545	5
GTCCA	95475	1.1666483	9.093142	1
ACATG	138070	1.161507	6.9558764	8
ATTGA	199335	1.1445404	5.4743943	8
GGACT	91265	1.1319598	8.228157	6
ACACT	135965	1.1268685	5.7916164	6
AGACT	125655	1.0570664	6.4212117	6
TGTAT	183180	1.0427458	6.1246758	2
GTATG	121790	1.0310113	7.1452975	3
GAGTA	119060	1.0166347	5.6624694	1
CATAC	120340	0.9973695	6.838639	3
GAATA	155145	0.89853	5.0908623	1
TATAA	217370	0.8465313	5.278262	2
ATACT	142480	0.80598146	5.310877	6
GTATA	139395	0.80037737	8.007995	1
>>END_MODULE