##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-168_GCTACGCT-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1140236	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4465873731403	34.0	31.0	34.0	31.0	34.0
2	32.62904170715536	34.0	31.0	34.0	31.0	34.0
3	32.73325609786044	34.0	31.0	34.0	31.0	34.0
4	36.24513258658734	37.0	37.0	37.0	35.0	37.0
5	35.97290210096857	37.0	35.0	37.0	35.0	37.0
6	36.07303926555555	37.0	36.0	37.0	35.0	37.0
7	36.0690795589685	37.0	36.0	37.0	35.0	37.0
8	36.08977878263798	37.0	36.0	37.0	35.0	37.0
9	37.949930540695085	39.0	38.0	39.0	35.0	39.0
10-14	38.120849017221	39.2	38.2	39.4	35.2	39.4
15-19	39.25141233919995	40.0	39.0	41.0	36.0	41.0
20-24	39.17584605292238	40.0	39.0	41.0	36.0	41.0
25-29	38.98232541333549	40.0	38.8	41.0	35.8	41.0
30-34	38.77272441845373	40.0	38.0	41.0	35.0	41.0
35-39	38.528300983305215	40.0	38.0	41.0	34.2	41.0
40-44	38.28446040995023	40.0	38.0	41.0	33.8	41.0
45-49	38.09623937500658	40.0	37.8	41.0	33.0	41.0
50-54	38.0361391852213	40.0	37.2	41.0	33.0	41.0
55-59	37.648462248166176	39.6	36.4	41.0	33.0	41.0
60-64	37.09624604029341	38.8	35.4	40.6	31.8	41.0
65-69	36.36087511708102	37.6	35.0	40.0	31.0	41.0
70-74	35.79429468987122	36.6	34.8	39.2	31.0	40.8
75-79	34.62762796473712	35.2	33.6	37.4	30.2	39.2
80-84	34.44148228963127	35.0	34.0	36.6	31.0	38.2
85-89	33.819878165572746	35.0	34.0	35.6	30.0	36.8
90-94	33.400656004546434	35.0	34.0	35.0	29.4	36.0
95-99	33.12622878070856	35.0	33.8	35.0	29.0	35.6
100-101	32.76497365457678	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	3.0
14	11.0
15	32.0
16	110.0
17	198.0
18	355.0
19	566.0
20	853.0
21	1264.0
22	1787.0
23	2413.0
24	3441.0
25	4364.0
26	5647.0
27	7509.0
28	9521.0
29	12191.0
30	15373.0
31	20075.0
32	26092.0
33	35293.0
34	50848.0
35	81646.0
36	146524.0
37	277336.0
38	362419.0
39	74299.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.76617822976998	23.102147274774694	10.637534685801885	24.49413980965344
2	15.330389134238814	23.269552098728617	40.022103421206126	21.377955345826443
3	15.98265622204526	27.39064544532886	32.60026871630083	24.02642961632504
4	10.99588155434489	18.44890005226988	44.30845895060321	26.246759442782018
5	10.256034715620276	39.76887240886974	39.391318990103805	10.58377388540618
6	25.443504677978947	44.49315755685665	19.209970567496555	10.85336719766785
7	24.12675972342568	33.53174255154196	26.685089753349306	15.656407971683056
8	21.001003301071005	39.22161727922992	23.802177794772312	15.975201624926768
9	22.96305326265791	14.280903251607564	24.37293683062103	38.3831066551135
10-14	20.471516422915958	25.561041749251913	33.106760354873906	20.860681472958227
15-19	19.810886215651035	31.612378484360438	28.44291242524712	20.133822874741412
20-24	19.792550261877448	29.763082471225054	30.301185376959694	20.143181889937804
25-29	19.82329795514802	30.476366075186263	29.55748877630448	20.142847193361234
30-34	19.633952679916636	30.165645376002953	29.644945887352865	20.555456056727547
35-39	19.86562804010944	30.512907260606077	29.520068056337394	20.101396642947087
40-44	19.459141588512956	30.20865908346679	30.08864264372403	20.24355668429622
45-49	19.781351010475476	30.133966685484957	29.832564018014317	20.252118286025254
50-54	19.513844285568883	29.56692954839796	30.440177734724607	20.47904843130855
55-59	19.806337827364015	29.532209150092836	30.481305755345815	20.18014726719733
60-64	19.310902287240868	29.11496012030333	30.830991830317416	20.743145762138393
65-69	19.791816006046282	29.474570468504357	30.014517323134065	20.719096202315296
70-74	19.523071801116377	29.260164372835224	30.167224995448123	21.049538830600277
75-79	19.839451613157845	29.65912628911865	29.587788991042935	20.91363310668057
80-84	19.60797520508948	29.50140058970352	29.4738097618559	21.4168144433511
85-89	19.680728464484513	29.517093382924823	29.291079530564758	21.511098622025905
90-94	19.504786515245705	28.96950297981974	29.916597322301847	21.60911318263271
95-99	19.689126896145265	28.93226499024739	29.502125927900874	21.876482185706468
100-101	19.55091733242826	27.93133523732597	30.67309340921239	21.844654021033374
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.26031803942341
2	36.70834448006526
3	40.009085838370304
4	37.242640997126905
5	20.839808601026455
6	36.296871875646794
7	39.78316769510874
8	36.97620492599778
9	61.3461599177714
10-14	41.33219789587419
15-19	39.94470909039244
20-24	39.93573215181525
25-29	39.966145148509256
30-34	40.18940873664418
35-39	39.96702468305653
40-44	39.70269827280918
45-49	40.033469296500726
50-54	39.99289271687743
55-59	39.986485094561345
60-64	40.05404804937926
65-69	40.510912208361574
70-74	40.57261063171666
75-79	40.75308471983842
80-84	41.02478964844059
85-89	41.19182708651042
90-94	41.113899697878416
95-99	41.56560908185173
100-101	41.39557135346163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	4.0
7	7.0
8	7.5
9	9.0
10	17.0
11	26.5
12	48.0
13	84.5
14	122.0
15	170.0
16	279.5
17	460.0
18	672.5
19	956.0
20	1397.0
21	2052.5
22	2909.0
23	4061.5
24	5484.5
25	7168.0
26	9334.5
27	11930.5
28	14951.0
29	18103.5
30	21582.5
31	25357.0
32	29398.5
33	34183.0
34	39945.0
35	46795.5
36	55039.0
37	63028.0
38	68699.5
39	69562.0
40	65609.0
41	60139.0
42	53594.0
43	47700.0
44	43909.5
45	40349.0
46	36886.0
47	33732.5
48	29678.5
49	25370.0
50	21883.5
51	19451.5
52	17344.0
53	15328.0
54	13506.0
55	11668.5
56	10302.0
57	9120.5
58	7718.5
59	6513.5
60	5514.0
61	4771.0
62	4096.5
63	3203.5
64	2613.5
65	2325.0
66	2032.0
67	1763.0
68	1554.0
69	1463.0
70	1413.0
71	1253.0
72	1061.0
73	921.5
74	736.5
75	548.0
76	387.5
77	263.5
78	191.5
79	152.0
80	114.5
81	75.0
82	53.0
83	35.0
84	19.0
85	9.5
86	5.5
87	4.0
88	3.0
89	2.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012365861102438442
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010874941678740192
20-24	7.016091405638833E-4
25-29	0.01689124005907549
30-34	0.006788068434955571
35-39	0.04160542203543827
40-44	0.049007398468387246
45-49	0.04951606509529606
50-54	0.04911263983947183
55-59	0.04011450261174003
60-64	0.05116484657562119
65-69	0.04334190465833389
70-74	0.004174574386355105
75-79	0.001666321708839223
80-84	1.7540228514097082E-4
85-89	0.00354312615984761
90-94	0.002876597476311921
95-99	0.0024556319919735916
100-101	4.3850571285242706E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1140236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.261731545012587
#Duplication Level	Relative count
1	100.0
2	7.298287774106338
3	2.9660558726344246
4	2.049864824271553
5	1.400420546710724
6	1.0327425653349354
7	0.8579152898768398
8	0.6350255331931511
9	0.5461099429258035
10++	3.369780714929408
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1477	0.12953458757660696	No Hit
ATCATACACATGACATCAAGTCATATTCGACTCCAAAACACTAACCAACC	1188	0.10418895737373667	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	315710	2.8338761	5.091824	5
CAAAG	289185	2.4792428	5.24563	4
CCGAG	133500	2.4189072	5.0485153	85-89
CTTTG	280985	2.3029613	10.609863	9
ACACA	275345	2.2546089	9.361228	6
AAAGC	236060	2.023791	5.066896	5
TACAC	244840	1.974982	11.717031	5
GCTTT	237035	1.9427459	5.2699537	1
CACAT	230205	1.8569299	7.137377	7
GCCAA	145460	1.7717791	5.422111	1
AGAGA	194385	1.7448386	5.0091357	8
TGGCT	139205	1.7228347	6.14446	6
TTGGC	138575	1.7150377	5.4724097	5
TGGAG	130275	1.7136114	5.076441	5
GAGAG	123385	1.6475055	5.865266	7
ATACA	288955	1.6405454	6.1274276	4
TTGAG	186225	1.6221985	6.7109637	9
GACTC	133440	1.601175	6.2888823	7
GAGTC	124640	1.5658835	8.692025	9
ATGGA	172430	1.524727	5.5133457	4
CATGA	175250	1.4800898	6.8177214	9
AAGAC	172575	1.4795212	6.4302607	5
ACGCT	122055	1.4645641	5.1737375	90-94
GTCTT	177575	1.45541	7.0782714	1
CCATG	119805	1.4375657	6.3063354	9
TATGA	244790	1.4334669	7.929758	4
GACTT	168425	1.4012748	8.035282	7
TCATA	249960	1.3980262	8.347822	2
TGAGT	159760	1.3916628	6.064785	8
ATGAG	154975	1.3703796	5.830599	7
GTGTT	156495	1.3429295	6.2458735	1
ACTCA	165285	1.3332582	5.846783	8
TATAC	236455	1.3224927	6.567709	5
CGCTA	109075	1.308814	5.118096	95-97
GATTG	149950	1.3062086	7.344473	7
GTGTA	149235	1.2999802	12.955064	1
AACAC	157230	1.2874472	5.3555117	5
GTTCT	149505	1.2253474	5.262005	1
GTATG	139440	1.2146562	7.4373865	3
TGGAC	96675	1.2145522	8.685934	5
ACATG	139845	1.1810737	7.456499	8
ACACC	101065	1.1757569	5.7081094	6
ACCAT	144375	1.1645892	5.283614	8
GTCCA	95980	1.1516846	8.477584	1
ATTGA	194955	1.1416378	5.3688407	8
GGACT	89715	1.1271119	8.271737	6
ACACT	138700	1.1188123	5.4948025	6
TATGG	127210	1.1081212	5.1187463	3
TGTAT	184585	1.0648223	6.030999	2
AGACT	124755	1.0536296	6.309975	6
GAGTA	116385	1.0291443	5.561334	1
TGTAG	114655	0.9987551	5.0096855	2
CATAC	120425	0.97139853	7.2586155	3
GAATA	152210	0.9047956	5.1164103	1
GGATA	99650	0.8811636	5.13255	1
TATAA	213410	0.8401112	5.0222993	2
ATACT	143500	0.80259544	5.4667635	6
GTATA	136830	0.8012635	7.6185875	1
>>END_MODULE