##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-167_GCTACGCT-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3243511	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749001005391996	33.0	31.0	34.0	30.0	34.0
2	31.94551120683728	33.0	31.0	34.0	30.0	34.0
3	32.09575580289384	34.0	31.0	34.0	30.0	34.0
4	35.6222359042408	37.0	35.0	37.0	33.0	37.0
5	35.48255054476461	37.0	35.0	37.0	33.0	37.0
6	35.615165479629944	37.0	35.0	37.0	33.0	37.0
7	35.63492832304253	37.0	35.0	37.0	33.0	37.0
8	35.68916307051217	37.0	35.0	37.0	33.0	37.0
9	37.5076656129731	39.0	38.0	39.0	35.0	39.0
10-14	37.641299258735366	39.2	37.2	39.4	34.6	39.4
15-19	38.7006592547397	40.0	38.2	41.0	34.6	41.0
20-24	38.593599220104394	40.0	38.0	41.0	34.0	41.0
25-29	38.40534649026934	40.0	38.0	41.0	34.0	41.0
30-34	38.12706829112033	40.0	38.0	41.0	33.2	41.0
35-39	37.794725715436144	40.0	37.8	41.0	32.6	41.0
40-44	37.73667405475116	40.0	37.6	41.0	32.6	41.0
45-49	37.56144764115182	40.0	37.0	41.0	32.0	41.0
50-54	36.81409028672941	39.0	35.6	40.2	31.0	40.6
55-59	37.41469339860417	39.8	36.0	41.0	32.0	41.0
60-64	36.942545038385866	39.0	35.0	41.0	31.0	41.0
65-69	36.259428563676835	37.8	35.0	40.2	30.6	41.0
70-74	35.402188615978176	36.4	34.6	39.2	29.6	41.0
75-79	34.429274511478454	35.2	34.0	37.6	29.0	39.4
80-84	33.49463319224137	35.0	33.6	36.2	27.8	38.0
85-89	32.69969943064784	35.0	33.0	35.2	25.8	36.6
90-94	32.06843676497475	35.0	32.4	35.0	24.8	36.0
95-99	31.469061951693703	34.8	31.6	35.0	22.6	35.0
100-101	30.493735029725507	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	81.0
7	343.0
8	759.0
9	1089.0
10	1295.0
11	1658.0
12	2153.0
13	2827.0
14	3402.0
15	4255.0
16	5028.0
17	5871.0
18	6579.0
19	7774.0
20	9191.0
21	10828.0
22	12270.0
23	14452.0
24	16727.0
25	19832.0
26	23314.0
27	28344.0
28	34114.0
29	41054.0
30	51157.0
31	64589.0
32	83939.0
33	113270.0
34	161526.0
35	250359.0
36	427840.0
37	757916.0
38	896671.0
39	182875.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2758994616307	22.832304871970745	10.331441309851023	24.560354356547528
2	15.417290036537107	23.84179651418662	39.22991114454255	21.511002304733726
3	15.844394552452384	27.93696955491957	32.84290807263397	23.37572781999408
4	11.724640081267943	18.53570290818468	43.617249947341755	26.122407063205628
5	11.535837672162758	38.31381194204586	37.971013062441514	12.179337323349863
6	25.515785501307096	43.04717413643678	19.690601152907814	11.746439209348313
7	23.529183252468716	33.906440815085745	26.829581866676204	15.73479406576934
8	21.421744369173545	37.36765415015619	24.717086658551715	16.493514822118545
9	23.0323606615804	15.204534845935234	25.081717129550558	36.68138736293381
10-14	20.797195882869737	25.68797308854637	32.50403710653897	21.01079392204492
15-19	20.21174442226074	31.126505317787785	28.406209279948445	20.255540980003026
20-24	20.055672030632817	29.734771508955767	29.911480311819616	20.2980761485918
25-29	20.087496083629322	30.148497430164046	29.4944195918613	20.269586894345338
30-34	19.93996873816266	29.95748284061316	29.6005905328886	20.501957888335586
35-39	20.09592518676546	30.142928885938826	29.51171133572052	20.2494345915752
40-44	19.770097675001455	29.73174649691207	30.071855114592243	20.42630071349423
45-49	19.736894684366863	29.372364667164273	30.347823234194415	20.542917414274445
50-54	19.30726774418029	29.20055954819289	30.680020016458386	20.812152691168432
55-59	19.296799316760037	29.21923184835526	30.66589997440933	20.81806886047537
60-64	19.216489024397088	28.696170883400413	30.716943302744497	21.370396789458006
65-69	19.60272229265016	28.677732116792466	30.11242075006864	21.607124840488737
70-74	19.58893805121441	28.4055069342397	29.98608841114755	22.019466603398342
75-79	19.534601144617916	28.422166394789027	29.827294747284526	22.215937713308527
80-84	19.612397311564607	28.111826598420777	29.906306101188374	22.369469988826246
85-89	19.755251633540745	27.794131993816972	30.0940145419321	22.356601830710186
90-94	20.075301977531016	27.243321565369428	30.392094710731083	22.289281746368474
95-99	20.629298956441026	26.80566191580166	29.977348094031925	22.58769103372539
100-101	21.016573199002185	26.254590840718734	30.31936665321834	22.409469307060743
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.83625381817822
2	36.92829234127083
3	39.22012237244646
4	37.847047144473564
5	23.71517499551262
6	37.26222471065541
7	39.263977318238055
8	37.91525919129209
9	59.71374802451421
10-14	41.807989804914655
15-19	40.46728540226376
20-24	40.35374817922461
25-29	40.357082977974656
30-34	40.44192662649825
35-39	40.34535977834066
40-44	40.19639838849568
45-49	40.2798120986413
50-54	40.119420435348715
55-59	40.11486817723541
60-64	40.58688581385509
65-69	41.2098471331389
70-74	41.608404654612755
75-79	41.75053885792644
80-84	41.98186730039085
85-89	42.111853464250935
90-94	42.36458372389949
95-99	43.21698999016642
100-101	43.42604250606292
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	4.5
2	11.0
3	17.0
4	22.0
5	28.0
6	45.0
7	59.0
8	69.0
9	87.5
10	124.5
11	173.5
12	238.5
13	345.5
14	500.0
15	698.5
16	985.0
17	1448.0
18	2156.0
19	3125.0
20	4576.5
21	6709.0
22	9540.5
23	13333.0
24	17802.0
25	22907.0
26	28743.5
27	35446.5
28	43172.0
29	51563.0
30	60447.0
31	70031.0
32	79750.5
33	90564.5
34	103769.5
35	118556.0
36	135842.5
37	152782.0
38	164574.5
39	169603.5
40	168058.0
41	162166.5
42	152818.0
43	142879.5
44	134222.0
45	125099.5
46	115870.0
47	105430.5
48	94078.5
49	82689.5
50	72385.5
51	64759.5
52	57779.5
53	51198.5
54	45315.0
55	40121.5
56	35511.5
57	30852.5
58	26376.0
59	22842.0
60	19650.0
61	16613.5
62	14092.0
63	11272.5
64	9063.0
65	8000.5
66	6951.5
67	5754.5
68	5007.5
69	4656.0
70	4258.5
71	3656.5
72	3062.5
73	2503.5
74	1998.5
75	1635.5
76	1288.5
77	956.0
78	707.5
79	513.5
80	377.0
81	267.0
82	195.5
83	150.5
84	105.5
85	80.5
86	70.0
87	56.0
88	45.5
89	34.0
90	32.5
91	28.5
92	21.0
93	21.5
94	17.5
95	12.5
96	15.0
97	14.5
98	9.5
99	6.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03474629807020849
2	0.032495650546583627
3	0.02932007938311293
4	0.027871032347354455
5	0.033451404974424316
6	0.0018498472796916673
7	7.39938911876667E-4
8	0.003545540619409029
9	1.541539399743056E-4
10-14	0.006869099565255058
15-19	0.024368654831138235
20-24	0.02040381549499909
25-29	0.0022938106268176675
30-34	0.002084161268452612
35-39	0.005913345137414364
40-44	0.013238740364993366
45-49	0.008552460589774475
50-54	0.005549541839075002
55-59	0.004347141107275418
60-64	0.01064278801582606
65-69	0.014792612079934367
70-74	0.01859713131850023
75-79	0.008102331085049503
80-84	0.01158004397086984
85-89	0.009576042751203865
90-94	0.010969594368571587
95-99	0.010488634075851754
100-101	0.007168158208805211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3243511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.35998799127601
#Duplication Level	Relative count
1	100.0
2	8.583594368081506
3	2.889928025162249
4	1.9984434396125323
5	1.4883804696695846
6	1.2516803776910164
7	1.1249686436698805
8	0.978960706498318
9	0.8374552167285217
10++	9.487943089064842
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	720165	4.243607	9.44576	80-84
GTCTC	1024670	4.0212226	6.4708796	60-64
GACGC	652705	4.0107603	9.410377	75-79
CGCTG	660090	3.8896122	9.085527	90-94
CTGTC	982895	3.8572807	6.195886	55-59
GCCGA	613035	3.7669954	9.54315	80-84
CGACG	604180	3.712583	9.653611	95-97
CTGCC	649300	3.6067874	8.795087	80-84
TGCCG	595505	3.5090415	9.048446	80-84
TCTCT	1341130	3.5052252	5.2498755	60-64
CCGAC	581865	3.3705754	8.859735	95-97
CTCTT	1258805	3.2900577	4.9578214	60-64
ACACA	1088920	3.2274618	5.8714027	6
TGTCT	1158920	3.2131171	4.9241934	80-84
TGACG	702385	3.0491865	6.770767	75-79
CTGAC	733380	3.001303	6.365076	95-97
GACGA	646230	2.9255164	7.3989534	85-89
TACAC	1019160	2.8966813	6.8192677	5
ATCTG	966670	2.7948463	5.2157087	70-74
ACGCT	655720	2.6834853	6.2835402	75-79
AGAAG	784000	2.614792	5.076908	5
ATACA	1144795	2.397131	5.0101166	6
CCTTG	598695	2.3495233	6.1875052	95-97
CTCCT	620580	2.295852	5.913743	90-94
ACTCC	591750	2.2829218	5.941284	90-94
CAAAG	676170	2.12593	5.1245728	4
TATAC	1041640	2.0915825	5.1965733	5
GGTGG	295495	1.9593376	7.5310106	95-97
CTTTG	669390	1.8558902	7.987889	9
GAGAG	357465	1.7166309	5.5519695	7
GTGTA	542470	1.6637325	8.184284	1
CTCGG	271785	1.6015062	7.148975	95-97
GCTTT	570835	1.5826457	5.1376886	1
CGCCG	173660	1.4484518	5.283436	95-97
CGGTG	223445	1.3966957	6.880159	95-97
AAGAC	421265	1.3244894	5.6187277	5
GAGTC	295945	1.2847534	6.804238	9
GTCTT	417360	1.1571347	6.0334215	1
GACTT	400070	1.1566865	5.836917	7
GTGTT	389435	1.1453443	5.7139297	1
TGAGT	373315	1.1449413	5.644502	8
GTTCT	379635	1.0525417	5.0610266	1
TGGAC	233940	1.015578	6.345023	5
GTCCA	233420	0.95525396	6.8709006	1
GGACT	217720	0.94516385	6.172447	6
AGACT	311140	0.9380851	5.172838	6
GTATA	332540	0.7083196	6.847525	1
>>END_MODULE