##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-167_GCTACGCT-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3243511	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.492611555810974	34.0	31.0	34.0	31.0	34.0
2	32.67620889832037	34.0	31.0	34.0	31.0	34.0
3	32.78873973296221	34.0	31.0	34.0	31.0	34.0
4	36.29205851313592	37.0	37.0	37.0	35.0	37.0
5	36.01913050395081	37.0	35.0	37.0	35.0	37.0
6	36.09953411596261	37.0	37.0	37.0	35.0	37.0
7	36.1014040032545	37.0	36.0	37.0	35.0	37.0
8	36.12626317592263	37.0	37.0	37.0	35.0	37.0
9	38.00429195399676	39.0	38.0	39.0	35.0	39.0
10-14	38.197543032843114	39.4	38.2	39.4	35.2	39.4
15-19	39.3388894935149	41.0	39.0	41.0	36.0	41.0
20-24	39.26705610062676	40.4	39.0	41.0	36.0	41.0
25-29	39.06913298582924	40.0	39.0	41.0	36.0	41.0
30-34	38.85805961502828	40.0	38.2	41.0	35.0	41.0
35-39	38.60988021930556	40.0	38.0	41.0	34.6	41.0
40-44	38.34542432567671	40.0	38.0	41.0	33.8	41.0
45-49	38.14123694971283	40.0	37.8	41.0	33.4	41.0
50-54	38.071867368416505	40.0	37.2	41.0	33.2	41.0
55-59	37.66730052711398	39.6	36.4	41.0	33.0	41.0
60-64	37.08838952604138	38.8	35.2	40.8	31.8	41.0
65-69	36.335913459211326	37.6	35.0	40.0	31.0	41.0
70-74	35.744817020814786	36.4	35.0	39.0	31.0	40.8
75-79	34.54686739153961	35.2	33.6	37.4	30.2	39.2
80-84	34.34976955527513	35.0	34.0	36.4	30.8	38.0
85-89	33.75203074692824	35.0	34.0	35.4	30.0	36.6
90-94	33.35834341243178	35.0	34.0	35.0	29.4	36.0
95-99	33.09390928533925	35.0	33.6	35.0	29.0	35.2
100-101	32.72148930587872	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	14.0
14	50.0
15	131.0
16	305.0
17	679.0
18	1221.0
19	1878.0
20	2809.0
21	4005.0
22	5500.0
23	7710.0
24	10008.0
25	13331.0
26	16833.0
27	21630.0
28	27279.0
29	34315.0
30	43320.0
31	54915.0
32	71246.0
33	95685.0
34	138928.0
35	225096.0
36	414766.0
37	787175.0
38	1034705.0
39	229738.0
40	235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.920159974792746	22.819962688580368	10.572586311561762	24.68729102506512
2	15.587700491722552	23.719700569426248	39.01924985715779	21.67334908169341
3	16.01745762539421	27.65401443065863	33.030133087262534	23.298394856684624
4	11.908176047499145	18.486510451174667	43.49253016253066	26.11278333879552
5	11.40951888247026	38.41513101080897	38.05922039419629	12.116129712524483
6	25.478162398709298	43.17081088980429	19.650835159800597	11.700191551685812
7	23.515690250472403	33.902181925697185	26.858025146207304	15.724102677623106
8	21.43498203027522	37.437579215855905	24.65568946737039	16.47174928649849
9	22.92993610935804	15.067961847516473	25.121881812640684	36.88022023048481
10-14	20.75402858199032	25.645314598902242	32.55334728323721	21.047309535870234
15-19	20.188523530787194	31.102023062647806	28.430360442665503	20.279092963899497
20-24	20.018809365441044	29.70327708056253	29.945238626558684	20.332674927437743
25-29	20.073469131499866	30.140111741807633	29.484786675992357	20.30163245070014
30-34	19.915227062615745	29.927864811239125	29.611945761895786	20.544962364249344
35-39	20.05257820554666	30.10883858807975	29.54827047795324	20.290312728420353
40-44	19.779166855088924	29.67805674827957	30.084049384172324	20.45872701245919
45-49	19.716836681341356	29.332952723875756	30.36199870800898	20.588211886773912
50-54	19.266131625386542	29.157586371895476	30.683285652274662	20.89299635044332
55-59	19.160616395153696	29.101917529188277	30.65443483179788	21.083031243860155
60-64	18.962480583119056	28.65612665291038	30.525372768948095	21.856019995022468
65-69	19.245366264055676	28.79504667202209	29.73447094369438	22.22511612022785
70-74	19.320234668482055	28.601232539841103	29.30891315983553	22.769619631841312
75-79	19.449374026039372	28.75974083952878	28.789419044663937	23.00146608976791
80-84	19.37010217160837	28.505186274176708	28.632018251373715	23.492693302841204
85-89	19.322552166454145	28.16705178762289	28.796040545889458	23.714355500033516
90-94	19.322997162993914	27.504992262486454	29.16244067371865	24.00956990080098
95-99	19.38688301026209	27.1527404973224	29.096692973359218	24.363683519056288
100-101	19.39360146976555	26.469869605786695	29.821223023634424	24.315305900813332
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.60745099985786
2	37.26104957341596
3	39.31585248207883
4	38.02095938629467
5	23.525648594994745
6	37.17835395039511
7	39.23979292809551
8	37.90673131677371
9	59.81015633984285
10-14	41.80133811786055
15-19	40.467616494686695
20-24	40.351484292878794
25-29	40.37510158220001
30-34	40.46018942686509
35-39	40.34289093396701
40-44	40.23789386754811
45-49	40.30504856811527
50-54	40.15912797582986
55-59	40.243647639013844
60-64	40.818500578141524
65-69	41.47048238428353
70-74	42.08985430032337
75-79	42.45084011580728
80-84	42.86279547444958
85-89	43.03690766648766
90-94	43.3325670637949
95-99	43.75056652931838
100-101	43.70890737057888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.5
5	1.5
6	4.0
7	7.0
8	18.0
9	26.5
10	42.5
11	77.5
12	132.5
13	230.0
14	368.0
15	551.0
16	870.5
17	1390.0
18	2119.0
19	3024.0
20	4431.5
21	6665.0
22	9556.5
23	13261.0
24	17507.0
25	22592.0
26	28478.0
27	34919.0
28	42540.0
29	51029.5
30	59713.0
31	69118.0
32	78791.0
33	89158.5
34	101538.5
35	115479.5
36	132156.0
37	148605.5
38	160287.5
39	164432.5
40	161456.0
41	154973.5
42	145530.5
43	136358.5
44	130308.0
45	123842.5
46	117119.0
47	110216.5
48	101110.5
49	89669.5
50	78926.5
51	70891.5
52	63179.0
53	55912.0
54	49359.5
55	43668.0
56	39011.0
57	33935.0
58	28429.5
59	24218.0
60	20593.0
61	17677.0
62	15132.5
63	11796.0
64	9301.0
65	8050.5
66	6942.0
67	5894.0
68	5138.5
69	4659.5
70	4314.5
71	3705.5
72	3030.0
73	2499.0
74	2056.0
75	1613.5
76	1176.0
77	884.5
78	604.5
79	401.5
80	281.0
81	192.0
82	119.0
83	76.5
84	51.5
85	32.0
86	18.0
87	12.5
88	7.5
89	5.0
90	5.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013010592533831394
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010050836886324726
20-24	6.289480750951669E-4
25-29	0.01759821378746673
30-34	0.006529960897311586
35-39	0.04338508486636857
40-44	0.05175872688577285
45-49	0.05219035791770091
50-54	0.05218419176010194
55-59	0.042238179552959734
60-64	0.05377506042063677
65-69	0.04440250087019899
70-74	0.0039155100753473625
75-79	0.001646364078925584
80-84	2.2198167356300008E-4
85-89	0.0035270421466121124
90-94	0.002830266337928251
95-99	0.0023986353060001953
100-101	1.541539399743056E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3243511.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.891013645309993
#Duplication Level	Relative count
1	100.0
2	8.807044083706678
3	2.9098220231053187
4	1.8877316835075606
5	1.5573786388937376
6	1.305896575577148
7	1.1759534014726893
8	0.9393404575809882
9	0.8216804690883937
10++	9.891843318270269
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	730470	4.3752728	9.579082	75-79
GAGCC	702870	4.2099586	9.342167	80-84
CGAGC	689155	4.12781	9.299715	85-89
GTCTC	1027325	3.9762633	6.4025564	60-64
CTCCG	751850	3.9762404	8.278916	75-79
CTGTC	988340	3.8253717	6.1463146	55-59
AGCCC	673605	3.6669443	8.490706	80-84
GCCCA	670215	3.6484897	8.61529	80-84
TCTCT	1350055	3.4756885	5.154711	60-64
TGTCT	1163570	3.2959886	5.0323334	80-84
ATCTC	1225730	3.2481923	8.044391	95-97
TCTCC	923300	3.2479277	6.126683	95-97
CTCTT	1258015	3.2387333	4.9100223	60-64
CCACG	592605	3.2259994	8.280924	90-94
CCCAC	637350	3.1533573	7.7355604	80-84
TCCGA	787685	3.1381772	6.5102606	75-79
ACGCT	781960	3.1153686	11.578254	95-97
ACACA	1090185	3.0609932	5.4766445	6
CGAGA	653945	2.9507163	7.436745	85-89
GAGAC	644960	2.9101744	7.4126782	85-89
CGCTA	725585	2.8907676	11.348175	90-94
GACGC	477695	2.861235	8.951612	90-94
ACGAG	625850	2.8239467	7.1271925	95-97
TACAC	1018300	2.7776675	6.6616054	5
AGAAG	779745	2.6504664	5.1254554	5
CACGA	612510	2.5118613	6.4278984	95-97
AGACG	543655	2.4530683	7.0075917	95-97
GCTAC	547420	2.1809492	6.6724424	90-94
TATAC	1047300	2.090747	5.341869	5
CAAAG	676265	2.0892134	5.046256	4
CTTTG	669640	1.8968569	8.395566	9
AGAGA	537320	1.8264287	5.0628295	8
GAGAG	352460	1.749846	5.9864335	7
CTACG	434870	1.7325442	6.00838	95-97
TCTCG	436125	1.6880227	5.7860003	90-94
TACGC	413785	1.6485406	6.1783743	95-97
GCTTT	573545	1.6246531	5.2581434	1
CTCGT	377775	1.462179	5.6504993	90-94
GTATG	440450	1.4130293	5.0803814	95-97
GTCTT	484890	1.3735244	5.951812	1
GAGTC	295410	1.2949526	6.7798758	9
AAGAC	418615	1.2932445	5.3353405	5
TGCCG	219615	1.2779304	8.036401	95-97
GCCGT	218320	1.2703949	7.805043	95-97
ATGCC	309310	1.2323068	5.8087664	95-97
GTGTT	387395	1.2073994	6.155718	1
TGAGT	375850	1.2057829	5.7800636	8
CCGTC	223100	1.1798886	6.5175695	95-97
GATTG	365305	1.171953	5.000782	7
GACTT	399050	1.1635313	6.061888	7
GTGTA	333910	1.071233	8.462931	1
GTTCT	377925	1.0705299	5.0713344	1
TGGAC	232665	1.0199051	6.495079	5
GGACT	215590	0.94505554	6.588595	6
GTCCA	236855	0.9436423	6.5782337	1
AGACT	309625	0.9292752	5.0101156	6
GTATA	333025	0.7314945	6.9275885	1
>>END_MODULE