##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-166_GCTACGCT-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3466647	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72254861830466	33.0	31.0	34.0	30.0	34.0
2	31.92468053424534	33.0	31.0	34.0	30.0	34.0
3	32.068580677525	34.0	31.0	34.0	30.0	34.0
4	35.596746654620446	37.0	35.0	37.0	33.0	37.0
5	35.46894650652345	37.0	35.0	37.0	33.0	37.0
6	35.60278938120899	37.0	35.0	37.0	33.0	37.0
7	35.61767408103565	37.0	35.0	37.0	33.0	37.0
8	35.668338310765414	37.0	35.0	37.0	33.0	37.0
9	37.481082440756154	39.0	38.0	39.0	35.0	39.0
10-14	37.60932220673175	39.2	37.2	39.4	34.4	39.4
15-19	38.67159684848212	40.0	38.0	41.0	34.4	41.0
20-24	38.571348798997995	40.0	38.0	41.0	34.0	41.0
25-29	38.39320807685352	40.0	38.0	41.0	34.0	41.0
30-34	38.12741054973292	40.0	38.0	41.0	33.2	41.0
35-39	37.812604456121434	40.0	37.8	41.0	32.6	41.0
40-44	37.76656717571764	40.0	37.8	41.0	32.8	41.0
45-49	37.60935670692747	40.0	37.0	41.0	32.0	41.0
50-54	36.89361299261217	39.2	36.4	40.2	31.2	40.6
55-59	37.516865893758435	40.0	36.6	41.0	32.0	41.0
60-64	37.06723995838053	39.0	35.4	41.0	31.4	41.0
65-69	36.393871830619034	38.0	35.0	40.2	31.0	41.0
70-74	35.543213716308586	36.6	34.8	39.2	30.0	41.0
75-79	34.56325769540423	35.4	34.0	37.6	29.0	39.4
80-84	33.600759119691155	35.0	33.8	36.4	28.2	38.2
85-89	32.75902403677098	35.0	33.0	35.4	26.2	36.6
90-94	32.10001081736906	35.0	32.4	35.0	24.8	36.0
95-99	31.46492832988187	35.0	31.6	35.0	22.2	35.2
100-101	30.433014668063983	34.0	30.0	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	8.0
6	64.0
7	363.0
8	860.0
9	1167.0
10	1286.0
11	1714.0
12	2417.0
13	3083.0
14	3796.0
15	4681.0
16	5579.0
17	6280.0
18	7179.0
19	8325.0
20	9630.0
21	11262.0
22	13157.0
23	15084.0
24	17799.0
25	20661.0
26	24459.0
27	29301.0
28	35523.0
29	42961.0
30	53196.0
31	67454.0
32	87803.0
33	119017.0
34	169616.0
35	259471.0
36	446765.0
37	812612.0
38	980733.0
39	203218.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.127683709071114	23.6274030852176	10.881828357466022	24.363084848245258
2	15.122380674405914	24.11630629463411	39.67867624632128	21.082636784638694
3	15.545267385812616	28.23912155894145	33.114319391404585	23.101291663841348
4	11.225680759829912	18.67212122838438	44.3542660506913	25.747931961094412
5	10.92995057561746	39.14054438383424	38.66849074418207	11.261014296366234
6	25.22098084861492	43.76133318333002	19.746090538540756	11.271595429514306
7	23.28956641063445	34.225258544271206	27.22867599313568	15.256499051958667
8	21.077982260080102	38.059565006179085	24.74617283209848	16.116279901642333
9	23.00993729657879	15.064051990444918	25.301956910977413	36.62405380199888
10-14	20.410288276686536	26.012268664363408	32.9109229423562	20.666520116593855
15-19	19.799006301632737	31.55835699934462	28.766995858561035	19.875640840461607
20-24	19.72726998893112	30.150673417479602	30.25469734962001	19.867359243969272
25-29	19.73909763452553	30.5067823837796	29.855492843528737	19.89862713816613
30-34	19.5838800781497	30.37731507871048	29.972425830017478	20.066379013122337
35-39	19.7066949008351	30.515143757647255	29.90893649856501	19.869224842952633
40-44	19.438393280725233	30.063416144488137	30.456124713070594	20.042065861716036
45-49	19.389954317455143	29.734688592934226	30.72423473544327	20.15112235416735
50-54	18.91019222388943	29.48101471116914	31.088731752969778	20.52006131197165
55-59	18.915769706154045	29.48511748178294	31.081890760586013	20.517222051477003
60-64	18.751825443359877	28.99115628587779	31.1240992219558	21.132919048806542
65-69	19.24798895097248	28.896304350485348	30.428199439193538	21.42750725934864
70-74	19.297483931381162	28.61615764338157	30.313273151171156	21.773085274066116
75-79	19.708484668399507	28.822555007111184	29.913961867890613	21.55499845659869
80-84	20.064711717417154	28.582137502677234	29.842773733207288	21.510377046698327
85-89	20.419299208562748	28.355233462420998	29.889722451427396	21.33574487758886
90-94	20.781404550521252	27.82264371786669	30.15662975451243	21.239321977099628
95-99	21.48278554787566	27.276493920737117	29.727862644881796	21.512857886505422
100-101	21.96955633531909	26.57391144303709	30.07021189345655	21.38632032818727
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.49076855731639
2	36.20501745904461
3	38.646559049653966
4	36.97361272092432
5	22.19096487198369
6	36.49257627812923
7	38.54606546259311
8	37.194262161722435
9	59.63399109857767
10-14	41.07680839328039
15-19	39.674647142094344
20-24	39.59462923290039
25-29	39.637724772691655
30-34	39.65025909127204
35-39	39.57591974378773
40-44	39.48045914244127
45-49	39.54107667162249
50-54	39.430253535861084
55-59	39.43299175763105
60-64	39.88474449216642
65-69	40.67549621032112
70-74	41.07056920544728
75-79	41.263483124998196
80-84	41.57508876411548
85-89	41.75504408615161
90-94	42.02072652762088
95-99	42.99564343438108
100-101	43.35587666350636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	8.5
2	16.0
3	26.0
4	31.0
5	39.0
6	57.5
7	74.5
8	86.5
9	109.5
10	141.0
11	173.5
12	247.0
13	342.0
14	476.5
15	666.5
16	950.5
17	1377.5
18	1970.5
19	2940.0
20	4425.5
21	6518.0
22	9518.0
23	13403.0
24	18128.5
25	23859.5
26	30779.0
27	38542.5
28	47697.0
29	57473.0
30	67614.0
31	78786.0
32	90649.5
33	102914.5
34	116680.5
35	133119.5
36	151638.0
37	169217.5
38	181960.5
39	187527.0
40	186067.5
41	180068.5
42	170327.0
43	160064.0
44	150308.0
45	139152.0
46	127630.5
47	114461.0
48	100604.5
49	87384.0
50	75264.0
51	65667.0
52	57245.5
53	49951.0
54	43399.5
55	37162.5
56	32098.5
57	27267.5
58	22404.0
59	18823.5
60	15562.0
61	12426.5
62	10035.0
63	7614.0
64	5751.0
65	4841.0
66	3981.5
67	3158.5
68	2661.5
69	2595.0
70	2472.0
71	2088.0
72	1737.5
73	1398.5
74	1125.0
75	898.5
76	686.5
77	521.0
78	380.5
79	259.5
80	200.5
81	158.5
82	111.5
83	79.0
84	64.0
85	50.0
86	35.5
87	34.0
88	36.5
89	31.0
90	19.0
91	13.0
92	12.5
93	13.5
94	13.5
95	10.5
96	9.0
97	11.0
98	7.0
99	3.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033288650387535854
2	0.03196171978283339
3	0.02775015742877772
4	0.026653997364023505
5	0.033202111435055255
6	0.0016442400971313203
7	7.500042548318303E-4
8	0.0033173265117561724
9	2.8846317493531936E-4
10-14	0.007355810960850644
15-19	0.02442706165352284
20-24	0.020590501426883095
25-29	0.0022557820279941973
30-34	0.002001934434051116
35-39	0.006023111092649468
40-44	0.013367383526502699
45-49	0.008371201336622967
50-54	0.00515772156784351
55-59	0.004142331192071186
60-64	0.010551982939133982
65-69	0.014302004213293133
70-74	0.01836356571638243
75-79	0.007990429945708347
80-84	0.01100198549203308
85-89	0.009242360124927632
90-94	0.009957748798767225
95-99	0.009767363103309914
100-101	0.006100996149882004
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3466647.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.085680308482832
#Duplication Level	Relative count
1	100.0
2	8.575328705909623
3	2.7481230602809936
4	1.8280040693856001
5	1.4519722354577416
6	1.2658879888735786
7	1.0810915225425934
8	0.9748496516554415
9	0.8589494288694577
10++	9.993818654784748
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	784010	4.5487175	10.626691	90-94
GTCTC	1176740	4.483311	7.233358	60-64
GACGC	737505	4.441986	10.74403	75-79
CTGTC	1126625	4.292376	7.143454	55-59
CGCTG	734485	4.2613797	10.552174	75-79
GCCGA	681730	4.106054	11.0265255	80-84
CGACG	665640	4.009144	11.034677	95-97
CTGCC	718105	3.9980118	10.209826	80-84
TGCCG	669235	3.8828082	10.510804	80-84
TCTCT	1523645	3.8120024	5.803444	60-64
CCGAC	653890	3.7792506	10.440272	80-84
CTCTT	1429365	3.5761235	5.5333414	60-64
TGTCT	1325650	3.4562845	5.3107758	55-59
ACACA	1228540	3.4386928	6.2885995	6
TGACG	798955	3.2930381	7.483942	75-79
CTGAC	830690	3.285506	7.1107416	85-89
TACAC	1145885	3.0895855	7.4981475	5
GACGA	717385	3.069528	8.077292	85-89
CACAT	1122160	3.0256171	5.3700147	95-97
ATCTG	1106985	2.9961755	5.7651486	70-74
ACGCT	743310	2.9399045	7.21341	75-79
CATCT	1128335	2.9305716	5.4125443	70-74
ACATC	1078065	2.9067266	5.3907666	70-74
TCTGA	1038085	2.8096905	5.404926	70-74
AGAAG	833660	2.534052	5.2299867	5
TGCAG	609375	2.51165	7.715187	90-94
TATAC	1189435	2.1946342	5.4363713	5
GCAGT	508000	2.0938144	7.222243	90-94
GTGTG	496155	2.0528548	7.40039	95-97
CAAAG	702230	2.0483053	5.172903	4
ATGCA	724405	2.0354097	5.483775	90-94
CAGTG	492260	2.028939	7.184799	95-97
ACGAT	691100	1.9418304	5.279907	85-89
GGTGG	303445	1.9119202	8.358132	95-97
CTTTG	695895	1.8143637	7.7721543	9
AGAGA	595550	1.8102759	5.1358194	8
AGTGT	625865	1.7652956	5.1784883	95-97
CGATA	622935	1.7503026	5.1602626	85-89
GAGAG	380440	1.6963552	5.66541	7
TATGC	623650	1.6879766	5.0566626	90-94
GTGTA	593650	1.674431	8.82911	1
CTCGG	282835	1.6409693	8.10911	95-97
CGGTG	227750	1.3770096	7.706658	95-97
TCTCG	356960	1.3599969	5.4039	95-97
AAGAC	445180	1.2985269	5.740281	5
GAGTC	305610	1.2596272	6.232298	9
CGCCG	147070	1.2468929	5.3814116	95-97
TATGA	611425	1.175644	5.3640018	4
GTCTT	439040	1.1446816	5.930337	1
GACTT	417740	1.1306589	5.5285773	7
GATTG	400155	1.1286649	5.163607	7
TCATA	609025	1.123716	5.3396626	2
GTGTT	399975	1.0867378	5.4182353	1
TGGAC	248025	1.0222802	6.6218247	5
TCGGT	254880	1.0119648	5.15321	95-97
GTCCA	245080	0.9693288	6.8666267	1
GGACT	226815	0.9348592	6.080247	6
AGACT	327660	0.9206485	5.0414996	6
GTATA	359885	0.6919846	6.931973	1
>>END_MODULE