##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-166_GCTACGCT-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3466647	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47137853955133	34.0	31.0	34.0	31.0	34.0
2	32.657379017823274	34.0	31.0	34.0	31.0	34.0
3	32.767988491473176	34.0	31.0	34.0	31.0	34.0
4	36.277976096210544	37.0	37.0	37.0	35.0	37.0
5	36.00760590853352	37.0	35.0	37.0	35.0	37.0
6	36.09139090308301	37.0	36.0	37.0	35.0	37.0
7	36.09278994948144	37.0	36.0	37.0	35.0	37.0
8	36.11441949526444	37.0	36.0	37.0	35.0	37.0
9	37.988559550482066	39.0	38.0	39.0	35.0	39.0
10-14	38.17979373152213	39.4	38.2	39.4	35.2	39.4
15-19	39.31459315009575	41.0	39.0	41.0	36.0	41.0
20-24	39.24464042632549	40.2	39.0	41.0	36.0	41.0
25-29	39.05330470624786	40.0	39.0	41.0	36.0	41.0
30-34	38.848335293440606	40.0	38.0	41.0	35.0	41.0
35-39	38.61357178853226	40.0	38.0	41.0	34.6	41.0
40-44	38.36947759607482	40.0	38.0	41.0	34.0	41.0
45-49	38.18947184411911	40.0	38.0	41.0	33.4	41.0
50-54	38.138719344657815	40.0	37.4	41.0	33.4	41.0
55-59	37.75659281143999	39.8	36.6	41.0	33.0	41.0
60-64	37.18926743911336	39.0	35.4	40.8	32.0	41.0
65-69	36.43309889931106	37.6	35.0	40.0	31.0	41.0
70-74	35.835870626573744	36.6	35.0	39.2	31.0	41.0
75-79	34.60727134894323	35.2	33.6	37.4	30.2	39.2
80-84	34.39133197005636	35.0	34.0	36.4	30.8	38.2
85-89	33.76753237350097	35.0	34.0	35.4	30.0	36.6
90-94	33.360194966490674	35.0	34.0	35.0	29.4	36.0
95-99	33.0862459315875	35.0	33.6	35.0	29.0	35.4
100-101	32.712409570400446	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	10.0
14	41.0
15	140.0
16	357.0
17	621.0
18	1221.0
19	1979.0
20	2917.0
21	4242.0
22	5855.0
23	8155.0
24	11005.0
25	13775.0
26	17987.0
27	22945.0
28	29055.0
29	36617.0
30	45995.0
31	57976.0
32	75323.0
33	101153.0
34	146135.0
35	232237.0
36	428639.0
37	848893.0
38	1128085.0
39	245083.0
40	202.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72903875127753	23.63733601950242	11.183832677512305	24.449792551707745
2	15.279547653056428	23.953887107713975	39.54175609004587	21.22480914918373
3	15.580934545686365	27.930216142572345	33.38563170694911	23.10321760479218
4	11.401708913540952	18.621221024234657	44.13829847688559	25.838771585338797
5	10.823888327828014	39.20015507780285	38.79841241407043	11.177544180298716
6	25.20966224712236	43.92607611908568	19.641284503440932	11.222977130351028
7	23.23544912418253	34.19857862655182	27.2338083456435	15.332163903622146
8	21.0221865681738	38.118216247572946	24.67623614403197	16.183361040221286
9	22.922524272012698	14.901632615031183	25.308835886665126	36.867007226290994
10-14	20.35906742163249	25.95272607796525	32.98398712069617	20.704219379706096
15-19	19.742000115271242	31.535471205238135	28.802885081091702	19.919643598398917
20-24	19.687152706082188	30.088037809232393	30.31767908387647	19.90713040080895
25-29	19.722074472842458	30.461868215605897	29.877525028565415	19.93853228298623
30-34	19.554506558262077	30.319920134349506	30.021260542644928	20.10431276474349
35-39	19.692597317133618	30.429903648733834	29.96051573108634	19.916983303046205
40-44	19.410888243681182	30.003893795543295	30.488736564356312	20.096481396419207
45-49	19.359019713878883	29.638853347560257	30.774080613793984	20.228046324766876
50-54	18.861460606494735	29.41180205437273	31.13912525550654	20.587612083626
55-59	18.755440715642223	29.355367359948193	31.063100614173578	20.826091310236006
60-64	18.473103823107838	28.909286154948727	30.970125389736086	21.647484632207348
65-69	18.834246079761595	28.9803224475595	30.047717464404965	22.13771400827394
70-74	18.83947840956145	28.81025429359918	29.583311866082667	22.76695543075671
75-79	19.14484521097037	28.932176055829757	28.96221705455248	22.960761678647394
80-84	18.98137707365718	28.64242937183285	28.797865138039963	23.578328416470008
85-89	19.016803509727232	28.312277381578987	28.902153274035925	23.76876583465786
90-94	19.015521618471993	27.599556516944503	29.263437231379847	24.121484633203664
95-99	19.090222742544842	27.129282247867575	29.27334568907246	24.50714932051512
100-101	19.074084864736694	26.299224091528238	30.019073190628635	24.60761785310643
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.17883130298527
2	36.50435680224015
3	38.684152150478546
4	37.240480498879755
5	22.00143250812673
6	36.43263937747339
7	38.567613027804676
8	37.20554760839509
9	59.789531498303695
10-14	41.063286801338585
15-19	39.66164371367016
20-24	39.59428310689114
25-29	39.66060675582869
30-34	39.65881932300556
35-39	39.60958062017983
40-44	39.50736964010039
45-49	39.587066038645766
50-54	39.44907269012074
55-59	39.581532025878225
60-64	40.120588455315186
65-69	40.97196008803553
70-74	41.60643384031816
75-79	42.10560688961776
80-84	42.559705490127186
85-89	42.78556934438509
90-94	43.13700625167565
95-99	43.597372063059964
100-101	43.68170271784312
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.5
6	4.0
7	10.5
8	15.0
9	24.5
10	42.0
11	72.0
12	127.0
13	217.5
14	337.5
15	492.5
16	761.5
17	1199.5
18	1845.5
19	2756.5
20	4234.5
21	6459.0
22	9399.0
23	13216.0
24	17938.5
25	23580.5
26	30459.5
27	38318.0
28	47167.0
29	56472.5
30	66505.5
31	77637.5
32	88472.5
33	100147.0
34	114006.5
35	129627.0
36	147476.5
37	164187.5
38	175873.5
39	180333.5
40	177866.5
41	171572.5
42	162202.5
43	152750.5
44	145881.5
45	138685.0
46	130619.0
47	121310.5
48	109229.5
49	96064.5
50	84111.5
51	74241.0
52	64585.0
53	55650.0
54	48060.0
55	41393.0
56	35628.5
57	29864.0
58	24330.5
59	20040.5
60	16359.5
61	13293.0
62	10805.0
63	7954.5
64	5876.0
65	4848.0
66	4002.0
67	3194.5
68	2635.5
69	2489.0
70	2406.5
71	2040.5
72	1675.5
73	1418.5
74	1141.0
75	910.0
76	652.0
77	447.5
78	302.0
79	206.0
80	162.0
81	118.0
82	74.5
83	43.5
84	27.0
85	19.5
86	16.0
87	11.5
88	6.0
89	3.5
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013211613412037625
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001176929753736103
20-24	6.576960388525281E-4
25-29	0.01636740054583002
30-34	0.006507729226540805
35-39	0.03946753159465039
40-44	0.04752142343884451
45-49	0.048081041998219026
50-54	0.04809258052521644
55-59	0.03865406544133279
60-64	0.05014066906725721
65-69	0.04328678403079402
70-74	0.0036461745311824364
75-79	0.001476931455668835
80-84	1.730779049611916E-4
85-89	0.0034961736802160705
90-94	0.002550014466428223
95-99	0.0023019361359838484
100-101	2.8846317493531934E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3466647.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.691857845778742
#Duplication Level	Relative count
1	100.0
2	8.673076798727458
3	2.855461652365649
4	1.8182876282726914
5	1.5150241511532418
6	1.2537907934639931
7	1.0636853301950844
8	0.9860912635547135
9	0.839309154160012
10++	10.318717628725324
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	820195	4.8227	10.955868	75-79
GAGCC	790980	4.6509175	10.90683	75-79
CGAGC	773375	4.5474005	10.794499	75-79
CTCCG	848535	4.3569307	9.324293	85-89
GTCTC	1179560	4.3438044	7.029602	60-64
CTGTC	1129430	4.1591973	6.908893	55-59
AGCCC	754315	3.993555	9.51093	90-94
GCCCA	746095	3.950036	9.736888	80-84
TCTCT	1534925	3.6501486	5.5450444	60-64
CCACG	668740	3.540497	9.548466	80-84
TGTCT	1331040	3.5154474	5.3860545	55-59
TCTCC	1047945	3.4747417	6.6623263	70-74
ATCTC	1397035	3.4255216	8.723257	95-97
CCCAC	718315	3.4241734	8.774457	80-84
CTCTT	1431660	3.404578	5.26456	60-64
ACGCT	889945	3.3791652	13.001716	90-94
TCCGA	884335	3.3578637	7.1684203	75-79
ACACA	1233330	3.2150724	6.119948	6
CGAGA	732980	3.1871357	8.336779	85-89
GAGAC	730555	3.1765919	8.421961	85-89
CGCTA	829860	3.1510196	12.812442	90-94
GACGC	535200	3.1469457	10.435359	85-89
ACGAG	704065	3.0614083	8.042069	95-97
TACAC	1151710	2.9117804	7.299762	5
CACAT	1126090	2.8470073	5.022934	95-97
CATCT	1135670	2.784656	5.1184163	70-74
ACATC	1081850	2.7351587	5.0397153	70-74
CACGA	688885	2.69705	7.161276	80-84
AGACG	615325	2.6755497	8.012079	85-89
AGAAG	825690	2.6549776	5.4675283	5
GCTAC	612385	2.3252559	7.3960414	90-94
TATAC	1193725	2.1645074	5.4305506	5
AGAGA	587740	1.8898574	5.3069477	8
CTACG	493720	1.8746793	6.8444233	90-94
CTTTG	696895	1.8405893	8.177677	9
GAGAG	378955	1.8300472	6.2844696	7
TACGC	468065	1.7772661	6.7328477	90-94
TCTCG	475180	1.7498804	6.337348	90-94
GCTTT	590835	1.560471	5.092114	1
CTCGT	411805	1.516498	6.2352076	90-94
AGGAG	301210	1.4546015	5.003693	7
GTATG	480860	1.454355	5.3899417	90-94
GCTAT	515845	1.4047686	5.0582533	95-97
GTCTT	511440	1.3507787	5.82212	1
AAGAC	444520	1.2869716	5.686583	5
TGCCG	225585	1.2864335	9.355405	95-97
GAGTC	304685	1.2848828	6.4611673	9
GCCGT	223035	1.2718916	8.814358	95-97
ATGCC	328270	1.2464576	6.4507437	95-97
TATGA	606385	1.2211511	5.5919027	4
GATTG	398250	1.2045019	5.7205677	7
CCATG	315320	1.1972857	5.0314984	9
CCGTC	228810	1.1748594	7.318966	95-97
GTGTT	394770	1.1579767	5.793384	1
GACTT	416335	1.1337793	5.853219	7
TCATA	611860	1.1094477	5.323915	2
GTGTA	351005	1.0616101	9.097367	1
TGGAC	244650	1.0317099	7.039809	5
GGACT	226220	0.953989	6.522507	6
GTCCA	249310	0.9466424	6.512437	1
GAGTA	300095	0.9358507	5.2745633	1
AGACT	327695	0.92013466	5.162516	6
GTATA	360590	0.72616386	7.016329	1
>>END_MODULE