##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-165_GCTACGCT-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2604350	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61185631731526	33.0	31.0	34.0	30.0	34.0
2	31.81099007429877	33.0	31.0	34.0	30.0	34.0
3	31.951352928753817	34.0	31.0	34.0	30.0	34.0
4	35.48160347111563	37.0	35.0	37.0	33.0	37.0
5	35.35826175437249	37.0	35.0	37.0	33.0	37.0
6	35.49131299556511	37.0	35.0	37.0	33.0	37.0
7	35.504641465240844	37.0	35.0	37.0	33.0	37.0
8	35.552694914278035	37.0	35.0	37.0	33.0	37.0
9	37.347641446042196	39.0	37.0	39.0	34.0	39.0
10-14	37.464398563941096	39.2	37.2	39.4	34.0	39.4
15-19	38.50521949814733	40.0	38.0	41.0	34.0	41.0
20-24	38.398925259661716	40.0	38.0	41.0	34.0	41.0
25-29	38.22028398640735	40.0	38.0	41.0	33.4	41.0
30-34	37.95514850154549	40.0	38.0	41.0	33.0	41.0
35-39	37.64746036439034	40.0	37.6	41.0	31.8	41.0
40-44	37.60145433601475	40.0	37.2	41.0	31.8	41.0
45-49	37.44423814003495	40.0	37.0	41.0	31.4	41.0
50-54	36.75347837272256	39.0	36.2	40.2	30.8	40.6
55-59	37.39492879221303	40.0	36.4	41.0	31.6	41.0
60-64	36.94360581335074	39.0	35.4	41.0	31.0	41.0
65-69	36.25403597826713	38.0	35.0	40.2	30.2	41.0
70-74	35.36252132009906	36.6	34.4	39.2	29.2	41.0
75-79	34.347900397412026	35.4	34.0	37.6	28.6	39.4
80-84	33.36841138863824	35.0	33.2	36.4	26.8	38.0
85-89	32.542914585213204	35.0	32.8	35.4	25.6	36.6
90-94	31.89423794804846	35.0	32.0	35.0	24.4	36.0
95-99	31.292512834296467	34.4	31.2	35.0	21.4	35.0
100-101	30.324658744024422	34.0	30.0	35.0	18.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	58.0
7	251.0
8	557.0
9	787.0
10	953.0
11	1234.0
12	1901.0
13	2672.0
14	3495.0
15	4086.0
16	4819.0
17	5822.0
18	6412.0
19	7447.0
20	8439.0
21	9793.0
22	11071.0
23	12934.0
24	14841.0
25	17183.0
26	20207.0
27	23779.0
28	28757.0
29	34522.0
30	42832.0
31	53111.0
32	68893.0
33	91555.0
34	129699.0
35	197673.0
36	338307.0
37	610435.0
38	711602.0
39	138137.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38060839510573	23.429518770641483	10.734339361152106	24.455533473100676
2	15.031596462548244	24.34790524595	39.84645793898572	20.77404035251604
3	15.284281732899462	28.730223111266795	33.16928770936846	22.81620744646528
4	10.984328513563236	18.782200829472625	44.73690477196416	25.49656588499997
5	10.60540237818952	39.40564083476703	39.04012008869909	10.948836698344364
6	24.839361207853198	44.29694849651921	19.823829143451764	11.039861152175833
7	23.06966592930463	34.25550304896194	27.67795466701788	14.996876354715551
8	20.73348536680988	38.52858269037816	24.88362296875378	15.854308974058185
9	23.123147799015026	15.048377051484179	24.978171002644054	36.850304146856736
10-14	20.250185185114074	26.04359731828147	33.20618504840925	20.500032448195206
15-19	19.673743868496505	31.70990308963713	28.88892371133767	19.727429330528693
20-24	19.598360147652155	30.232721050202333	30.424610167142934	19.74430863500258
25-29	19.59013235343019	30.67368289932675	29.948823565596477	19.787361181646585
30-34	19.424077830981457	30.512883859988833	30.059846651451256	20.003191657578455
35-39	19.601555678656403	30.658974647070437	29.95166017571234	19.78780949856082
40-44	19.309483442850016	30.248356097657634	30.51324991355563	19.928910545936716
45-49	19.28797264076395	29.926526083136807	30.72353609938882	20.061965176710427
50-54	18.90353107062602	29.721764414045644	31.001168882833703	20.373535632494637
55-59	18.960689020766683	29.713582813678613	31.02674680093442	20.29898136462029
60-64	18.834474203823394	29.17216096285184	31.114778199400767	20.878586633924
65-69	19.309101729019908	29.146029423308256	30.3880352197347	21.156833627937136
70-74	19.213520523710297	28.748487145082528	30.27977245095132	21.758219880255858
75-79	19.271384341641657	28.88503074004751	29.949863436020834	21.893721482289997
80-84	19.291047363942592	28.609783197996407	29.90256463388755	22.196604804173447
85-89	19.502517304862888	28.500331746780628	29.84896938843927	22.148181559917216
90-94	19.755098749265574	27.9034280952509	30.221752871466588	22.119720284016942
95-99	20.37461612227315	27.510629071776627	29.746585786543633	22.368169019406594
100-101	20.734979745922594	26.813871767824416	30.18312573655991	22.26802274969309
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.83614186820641
2	35.80563681506428
3	38.10048917936474
4	36.480894398563215
5	21.554239076533882
6	35.879222360029026
7	38.06654228402018
8	36.587794340868065
9	59.97345194587177
10-14	40.75021763330928
15-19	39.401173199025195
20-24	39.342668782654734
25-29	39.37749353507677
30-34	39.427269488559915
35-39	39.38936517721722
40-44	39.23839398878673
45-49	39.34993781747438
50-54	39.27706670312065
55-59	39.25967038538697
60-64	39.71306083774739
65-69	40.46593535695704
70-74	40.97174040396616
75-79	41.16510582393166
80-84	41.48765216811604
85-89	41.650698864780104
90-94	41.87481903328252
95-99	42.74278514167974
100-101	43.00300249561568
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	4.5
2	4.5
3	11.0
4	20.5
5	25.5
6	35.5
7	51.5
8	64.0
9	80.0
10	90.5
11	113.5
12	170.5
13	250.5
14	349.5
15	456.5
16	660.0
17	966.5
18	1429.5
19	2128.0
20	3101.5
21	4577.5
22	6626.5
23	9492.5
24	12974.0
25	17147.5
26	22405.5
27	28223.0
28	35057.0
29	42709.0
30	50898.0
31	60081.0
32	68745.5
33	78107.5
34	89631.0
35	102935.5
36	118141.5
37	132160.0
38	142309.0
39	145970.0
40	142942.0
41	136709.5
42	127636.5
43	118066.5
44	110774.0
45	103099.5
46	94843.5
47	86005.0
48	76115.0
49	65984.5
50	56839.5
51	49731.0
52	43410.5
53	37918.0
54	32702.5
55	27863.5
56	23782.0
57	19794.0
58	16057.0
59	12956.0
60	10267.5
61	8082.0
62	6349.5
63	4367.5
64	2845.5
65	2192.0
66	1677.5
67	1183.0
68	871.5
69	893.0
70	915.5
71	723.5
72	594.5
73	481.0
74	363.0
75	281.0
76	202.0
77	154.5
78	103.5
79	61.0
80	48.0
81	37.5
82	26.5
83	25.5
84	27.0
85	17.0
86	11.5
87	14.5
88	14.0
89	12.5
90	12.5
91	9.0
92	8.0
93	9.0
94	7.5
95	3.0
96	5.5
97	8.0
98	3.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03747576170637587
2	0.03432718336629101
3	0.03094822124522434
4	0.029489123965672815
5	0.03613185631731526
6	0.002303837809818189
7	8.063432334363661E-4
8	0.0038013323862000114
9	6.143567492848503E-4
10-14	0.007641062069230327
15-19	0.025557240770249776
20-24	0.021295140822086125
25-29	0.002511183212701826
30-34	0.0023268761879163707
35-39	0.006212682627143049
40-44	0.013945898208766102
45-49	0.009299825292299422
50-54	0.005913183711866684
55-59	0.004638393457100621
60-64	0.010973947434100639
65-69	0.015389636569585502
70-74	0.019129533280857026
75-79	0.00854723827442548
80-84	0.011741893370706701
85-89	0.009806669610459423
90-94	0.010751243112484881
95-99	0.010198322038128517
100-101	0.007026705319945475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2604350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.95423839616474
#Duplication Level	Relative count
1	100.0
2	8.262292562015231
3	2.9116575183659874
4	2.0559658235712317
5	1.5736785135559241
6	1.4109946134976399
7	1.1439112027720313
8	1.040152179900212
9	0.8774682798419277
10++	8.821438407491145
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	506150	3.984031	9.169775	80-84
GTCTC	751905	3.821265	6.2383623	60-64
GACGC	468020	3.812302	9.249924	75-79
CGCTG	463230	3.6461976	8.933798	75-79
CTGTC	705690	3.5863953	6.0241995	55-59
GCCGA	436935	3.5590963	9.297354	80-84
CGACG	422880	3.4446099	9.168203	85-89
CTGCC	461955	3.4210925	8.536162	80-84
TCTCT	1026590	3.3685412	5.0141807	60-64
TGCCG	421795	3.3200524	8.854393	80-84
CCGAC	421795	3.2325552	8.603198	80-84
CTCTT	955745	3.136078	4.769105	60-64
ACACA	834075	3.0331101	6.46671	6
TGTCT	867310	3.024806	4.581444	60-64
TGACG	511115	2.8570678	6.44531	75-79
CTGAC	536360	2.8208497	6.03858	75-79
GACTC	519605	2.732731	6.909142	85-89
GACGA	466885	2.7007928	6.8144765	85-89
TACAC	766355	2.6929834	7.592607	5
AGAAG	648285	2.6632123	5.207344	5
ACGCT	473430	2.4898853	6.016134	75-79
CTCCT	495980	2.3715372	5.762571	90-94
ACTCC	475765	2.3541694	5.708563	85-89
CGACT	428335	2.2527196	5.842469	85-89
ATACA	882920	2.2033496	5.1057673	6
ACGAC	396500	2.1579738	6.131628	85-89
CAAAG	555295	2.146274	5.028718	4
GGTGG	216820	1.9279668	7.3863387	95-97
CTTTG	548460	1.912794	8.483632	9
TATAC	790345	1.9058969	5.8666563	5
AGAGA	458050	1.88171	5.1579404	8
GAGAG	291460	1.7920027	5.838014	7
GTGTA	428145	1.6423732	9.379007	1
GCTTT	462395	1.6126359	5.0756283	1
CTCGG	195770	1.540954	7.05762	95-97
ACGTG	270930	1.5144643	5.8489156	95-97
CGTGT	257345	1.3900751	5.459795	95-97
AAGAC	350650	1.3552995	5.9155874	5
GAGTC	239880	1.3408988	7.11323	9
CGGTG	156205	1.3068231	6.6356626	95-97
CCATG	242455	1.2751307	5.134149	9
ATGAG	313920	1.2461759	5.0456853	7
TATGA	480010	1.2303009	5.7841268	4
GTCTT	344805	1.2025324	6.278553	1
GATTG	311540	1.195074	5.5100913	7
TGAGT	311390	1.1944985	5.4135027	8
GACTT	327620	1.1824235	6.1923175	7
TCATA	483180	1.1651763	5.4033766	2
GTGTT	307980	1.1416265	5.718394	1
CGCCG	98655	1.1315756	5.110678	95-97
GTTCT	321025	1.1195978	5.1348443	1
TGGAC	193205	1.0799915	7.1100335	5
GTCCA	191820	1.0088289	7.284086	1
GGACT	176515	0.9866964	6.863649	6
AGACT	257520	0.96181816	5.3539696	6
GAGTA	238190	0.94554865	5.1995945	1
GAATA	345515	0.91644704	5.0426764	1
GGATA	221845	0.8806635	5.1918917	1
GTATA	281160	0.7206337	7.242731	1
>>END_MODULE