##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-165_GCTACGCT-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2604350	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47894561022904	34.0	31.0	34.0	31.0	34.0
2	32.65971931576017	34.0	31.0	34.0	31.0	34.0
3	32.768956937431604	34.0	31.0	34.0	31.0	34.0
4	36.27491658187264	37.0	37.0	37.0	35.0	37.0
5	36.009604315856166	37.0	35.0	37.0	35.0	37.0
6	36.09713287384568	37.0	36.0	37.0	35.0	37.0
7	36.091424731698886	37.0	36.0	37.0	35.0	37.0
8	36.11580279148348	37.0	36.0	37.0	35.0	37.0
9	37.98855530170676	39.0	38.0	39.0	35.0	39.0
10-14	38.17202572618888	39.4	38.2	39.4	35.2	39.4
15-19	39.309222723520264	40.8	39.0	41.0	36.0	41.0
20-24	39.24168963464972	40.0	39.0	41.0	36.0	41.0
25-29	39.0549787854935	40.0	39.0	41.0	36.0	41.0
30-34	38.86125658993607	40.0	38.0	41.0	35.0	41.0
35-39	38.63706452665732	40.0	38.0	41.0	34.8	41.0
40-44	38.40598437230019	40.0	38.0	41.0	34.0	41.0
45-49	38.23909651160559	40.0	38.0	41.0	33.6	41.0
50-54	38.196715955996694	40.0	37.6	41.0	33.8	41.0
55-59	37.81849874248853	39.8	36.8	41.0	33.0	41.0
60-64	37.254644729011076	39.0	35.6	40.8	32.2	41.0
65-69	36.497187858774744	37.8	35.0	40.0	31.0	41.0
70-74	35.8872150056636	36.6	35.0	39.2	31.0	41.0
75-79	34.665033271257705	35.2	33.8	37.4	30.4	39.2
80-84	34.441016990803845	35.0	34.0	36.4	31.0	38.2
85-89	33.8196547315069	35.0	34.0	35.6	30.0	36.8
90-94	33.41223645055388	35.0	34.0	35.0	29.8	36.0
95-99	33.14751619405994	35.0	34.0	35.0	29.0	35.4
100-101	32.7853132259489	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	6.0
14	30.0
15	98.0
16	240.0
17	492.0
18	841.0
19	1305.0
20	1978.0
21	2908.0
22	4037.0
23	5697.0
24	7793.0
25	10075.0
26	12990.0
27	16910.0
28	21124.0
29	26893.0
30	33968.0
31	43442.0
32	55888.0
33	75156.0
34	107515.0
35	171831.0
36	319642.0
37	640966.0
38	857566.0
39	184780.0
40	175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.966498358515565	23.426920344807726	11.097586729894216	24.508994566782498
2	15.207800656378337	24.23350202343638	39.71607374233195	20.842623577853338
3	15.414287634150556	28.351642444371915	33.48766486839326	22.746405053084263
4	11.152034096799586	18.73358035594294	44.598229884616124	25.51615566264135
5	10.463148194367117	39.45068827154568	39.16401405341064	10.92214948067656
6	24.890740491869373	44.43788277305278	19.69339758481003	10.97797915026782
7	22.994835563576324	34.25822182118379	27.696776546931094	15.05016606830879
8	20.682819129533282	38.60226160078331	24.816134544128094	15.89878472555532
9	23.02378712538637	14.84347341947127	25.002668612129707	37.13007084301265
10-14	20.20532570507036	25.980102520782538	33.2992186150095	20.515353159137597
15-19	19.613247724917727	31.70759053963882	28.93702832758789	19.74213340785557
20-24	19.54702430640617	30.193216384687986	30.489077053864825	19.77068225504102
25-29	19.551562129042214	30.652558503175275	29.997042130704333	19.798837237078178
30-34	19.38778652722707	30.50200205316337	30.117788478707624	19.992422940901935
35-39	19.58247332310105	30.595687659136782	29.993059481183543	19.828779536578626
40-44	19.288011478213356	30.187343078504885	30.534181936811738	19.990463506470018
45-49	19.302706675618282	29.849520028729586	30.753151288106157	20.094622007545972
50-54	18.868275787090234	29.627185476042023	31.07372657399799	20.430812162869756
55-59	18.8272062099929	29.587429435390938	31.025211194458702	20.560153160157462
60-64	18.595175593446417	29.117420733906958	30.959176295720315	21.328227376926307
65-69	19.01749062844271	29.24343061344392	30.079427842331803	21.659650915781565
70-74	19.003472098759328	29.07708956242309	29.718856842336947	22.20058149648063
75-79	19.231179498017553	29.30745240325427	29.138086758001037	22.32328134072714
80-84	19.099079215483176	29.045421181622523	29.00551094548017	22.849988657414126
85-89	19.122302216838218	28.795135782598546	29.09323213908173	22.989329861481497
90-94	19.13556207595165	28.127854203895552	29.55614554328913	23.180438176863667
95-99	19.2169438082928	27.716103002732183	29.510551097992916	23.556402090982097
100-101	19.195917749855244	26.88920605080427	30.383343547022136	23.531532652318354
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.47549292529806
2	36.05042423423168
3	38.16069268723482
4	36.668189759440935
5	21.385297675043677
6	35.868719642137194
7	38.045001631885114
8	36.5816038550886
9	60.15385796839902
10-14	40.72067886420796
15-19	39.355381132773296
20-24	39.31770656144719
25-29	39.35039936612039
30-34	39.38020946812901
35-39	39.41125285967967
40-44	39.27847498468337
45-49	39.397328683164254
50-54	39.29908794995999
55-59	39.387359370150364
60-64	39.92340297037272
65-69	40.67714154422428
70-74	41.20405359523996
75-79	41.55446083874469
80-84	41.9490678728973
85-89	42.111632078319715
90-94	42.316000252815314
95-99	42.773345899274894
100-101	42.727450402173595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	3.0
7	7.0
8	12.5
9	21.0
10	32.0
11	51.5
12	82.5
13	132.0
14	230.5
15	361.0
16	553.0
17	866.0
18	1322.0
19	1981.0
20	2896.5
21	4466.5
22	6668.0
23	9453.5
24	12881.0
25	17110.0
26	22243.5
27	28104.0
28	34845.0
29	42414.5
30	50488.0
31	59192.5
32	68069.0
33	77439.0
34	88733.0
35	101753.5
36	116375.0
37	130366.5
38	140953.5
39	144438.5
40	140610.0
41	133770.0
42	124933.0
43	115917.5
44	109442.5
45	102885.0
46	96144.5
47	89114.5
48	80119.5
49	69969.5
50	60068.0
51	52354.0
52	45500.5
53	39438.5
54	34125.0
55	29003.5
56	24537.0
57	20441.5
58	16442.5
59	13067.0
60	10281.0
61	8120.5
62	6380.0
63	4417.5
64	2848.0
65	1985.5
66	1471.5
67	1070.5
68	787.5
69	838.5
70	852.0
71	668.0
72	581.0
73	510.0
74	394.5
75	282.0
76	174.0
77	112.5
78	61.0
79	31.0
80	23.0
81	11.5
82	4.5
83	3.0
84	2.5
85	1.5
86	2.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013976616046230345
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010213680956860638
20-24	6.143567492848503E-4
25-29	0.01722502735807399
30-34	0.006658091270374566
35-39	0.04171482327644134
40-44	0.04979361452953712
45-49	0.05065371397853591
50-54	0.05041565073818803
55-59	0.04077792923378194
60-64	0.052873077735327435
65-69	0.04636089619290802
70-74	0.003985639410985467
75-79	0.001681801601167278
80-84	2.1502486224969763E-4
85-89	0.003509512930289708
90-94	0.0029719507746654634
95-99	0.0023729529441127344
100-101	7.67945936606063E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2604350.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.69313290768891
#Duplication Level	Relative count
1	100.0
2	8.378251321780233
3	2.964909230244135
4	2.036926128423588
5	1.6711423658739206
6	1.393584616474437
7	1.217776575671516
8	1.0458324478532743
9	0.9099515078920423
10++	9.178725295106354
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	527285	4.237574	9.507654	75-79
GAGCC	517720	4.160704	9.371274	75-79
CGAGC	497765	4.000334	9.336985	75-79
CTCCG	546495	3.9226518	8.25227	75-79
GTCTC	755160	3.8048165	6.253743	60-64
AGCCC	490045	3.608843	8.405974	80-84
CTGTC	708995	3.5722177	5.9659896	55-59
GCCCA	483665	3.5618584	8.556653	80-84
TCTCT	1027870	3.3311503	4.985947	60-64
TCTCC	697565	3.2206204	5.973512	70-74
CCACG	430620	3.1712186	8.349557	80-84
CCCAC	464015	3.1312964	7.8703146	80-84
ATCTC	938860	3.12173	7.284907	95-97
CTCTT	956740	3.10063	4.7402205	60-64
TGTCT	871270	3.0814047	4.7046194	60-64
ACGCT	577350	2.9845054	11.065984	90-94
TCCGA	574465	2.9695919	6.233337	75-79
ACACA	835255	2.9234145	6.5299025	6
GAGAC	492105	2.8481925	7.2168183	85-89
CGAGA	491630	2.8454432	7.0866036	85-89
CGCTA	540250	2.7927237	10.918605	90-94
GACGC	345155	2.7738698	8.930575	85-89
AGAAG	649040	2.7053442	5.3554664	5
ACGAG	465750	2.6956553	6.7318797	85-89
TACAC	771120	2.6306005	7.967276	5
CACGA	451405	2.3940763	6.1211667	80-84
AGACG	408760	2.3658102	6.726267	85-89
GCTAC	424230	2.1929796	6.5338182	90-94
CTTTG	552165	1.9528319	8.882313	9
AGAGA	456120	1.9012104	5.4464083	8
TATAC	790330	1.8925291	5.651777	5
GAGAG	289535	1.8287421	6.3298326	7
TGGAG	279400	1.7200433	5.035335	5
CTACG	330140	1.7065984	5.8876553	90-94
TCTCG	331165	1.6685498	5.5905285	90-94
GCTTT	465020	1.6446277	5.1399107	1
TACGC	313365	1.6198832	5.8198886	95-97
ATGGA	374575	1.5217786	5.0445514	4
CTCGT	285195	1.4369334	5.548509	90-94
GTCTT	394080	1.3937355	6.0077105	1
GAGTC	241815	1.3641318	7.3492174	9
AAGAC	347620	1.327749	5.8574796	5
GACTC	250865	1.2968009	5.0903	7
ATGAG	315500	1.2817758	5.328197	7
TATGA	482530	1.2609512	5.6596584	4
GATTG	314865	1.2468053	5.5139017	7
TGAGT	313755	1.24241	5.6502132	8
GCCGT	154865	1.2130724	8.013738	95-97
AGAAC	313320	1.1967387	5.1241364	5
TGCCG	152425	1.1939597	8.232755	95-97
ATGCC	230770	1.1929233	5.7363405	95-97
GTGTT	308940	1.1923671	5.910454	1
GACTT	328245	1.1910568	6.3633113	7
CCGTC	162445	1.1660037	6.6937165	95-97
TCATA	483825	1.1585703	5.511054	2
GTTCT	320990	1.1352395	5.073025	1
GTGTA	279550	1.1069647	9.449318	1
TGGAC	193280	1.0903351	7.546145	5
GTCCA	194495	1.0054064	7.219357	1
GGACT	176405	0.99513954	7.094853	6
GAGTA	237165	0.9635257	5.366519	1
AGACT	252585	0.9403301	5.402314	6
GGATA	218285	0.8868223	5.1813307	1
GTATA	282310	0.7377347	7.301634	1
>>END_MODULE