##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-163_GCTACGCT-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1538541	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.811860067427517	33.0	31.0	34.0	30.0	34.0
2	32.01118852211283	33.0	31.0	34.0	30.0	34.0
3	32.15417528684643	34.0	31.0	34.0	30.0	34.0
4	35.68022821621263	37.0	35.0	37.0	35.0	37.0
5	35.55680739089826	37.0	35.0	37.0	33.0	37.0
6	35.684567392094195	37.0	35.0	37.0	33.0	37.0
7	35.70205213900702	37.0	35.0	37.0	35.0	37.0
8	35.75563862126521	37.0	35.0	37.0	35.0	37.0
9	37.58040962184303	39.0	38.0	39.0	35.0	39.0
10-14	37.71989878722764	39.2	37.8	39.4	35.2	39.4
15-19	38.80271334985548	40.0	38.4	41.0	35.2	41.0
20-24	38.70405676546806	40.0	38.6	41.0	34.6	41.0
25-29	38.53113566684281	40.0	38.0	41.0	34.0	41.0
30-34	38.274842334393426	40.0	38.0	41.0	33.8	41.0
35-39	37.97036660056508	40.0	38.0	41.0	33.0	41.0
40-44	37.94122041596552	40.0	38.0	41.0	33.0	41.0
45-49	37.79807063965146	40.0	37.4	41.0	32.8	41.0
50-54	37.076895188363515	39.2	36.4	40.2	31.6	40.6
55-59	37.69233358097055	40.0	36.8	41.0	32.8	41.0
60-64	37.2425821606314	39.2	35.6	41.0	32.0	41.0
65-69	36.56333656366648	38.2	35.0	40.4	31.0	41.0
70-74	35.69207307442571	36.6	35.0	39.4	30.4	41.0
75-79	34.66829379262561	35.4	34.0	37.8	29.4	39.6
80-84	33.6183024046808	35.0	33.8	36.4	28.2	38.2
85-89	32.653893006426216	35.0	33.0	35.4	25.6	36.6
90-94	31.846863229514195	35.0	32.2	35.0	23.8	36.0
95-99	31.085542081751477	35.0	31.2	35.0	19.4	35.2
100-101	29.992461689353746	34.0	30.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	43.0
7	141.0
8	318.0
9	451.0
10	526.0
11	650.0
12	942.0
13	1181.0
14	1508.0
15	1807.0
16	2162.0
17	2604.0
18	2885.0
19	3449.0
20	3951.0
21	4541.0
22	5290.0
23	6160.0
24	7444.0
25	8624.0
26	10304.0
27	12448.0
28	15198.0
29	18860.0
30	23499.0
31	30566.0
32	39959.0
33	54253.0
34	76403.0
35	116128.0
36	196115.0
37	354669.0
38	438021.0
39	97362.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04551705304634	23.49858094467147	11.047695213573515	24.408206788708675
2	15.115741538413523	24.060638254296542	39.794699614249055	21.02892059304088
3	15.522559236036523	28.31782919528452	33.07407137711594	23.08554019156301
4	11.121542356418315	18.59153482771758	44.49702687837183	25.78989593749228
5	10.791681241701982	39.24219352439308	39.01332754233046	10.952797691574482
6	25.24475042671267	43.93821018572564	19.729854807196073	11.087184580365628
7	23.305672748875388	34.04266797354099	27.396263058722898	15.255396218860717
8	21.0487419165923	38.18189381673341	24.62887335707521	16.14049090959908
9	23.13821023013481	14.9716645661809	25.001283690372585	36.888841513311704
10-14	20.437282945203325	25.892692086588692	32.9515604284974	20.71846453971058
15-19	19.793746819227586	31.52650290440116	28.75579495450504	19.923955321866213
20-24	19.76097950658531	30.115981472505204	30.25797757811151	19.86506144279797
25-29	19.746817079037992	30.41131662476677	29.906242943651517	19.935623352543715
30-34	19.611398647160723	30.308588998047725	30.005400021475293	20.07461233331626
35-39	19.738583605392908	30.327970402414174	30.01630602441497	19.917139967777956
40-44	19.41722025126344	29.91325181156594	30.561054998590688	20.108472938579933
45-49	19.31120195473405	29.650868846523604	30.814232013515173	20.22369718522717
50-54	18.844996103273964	29.41469809960669	31.108860680506815	20.631445116612532
55-59	18.864927453786574	29.37552576196241	31.05919902574058	20.700347758510436
60-64	18.768775425275123	28.827222600180995	31.08791153502586	21.316090439518025
65-69	19.298069071087188	28.832140275935398	30.245906466848243	21.623884186129175
70-74	19.642164570077288	28.779786410647564	29.77951324489836	21.79853577437679
75-79	20.277209053930534	29.29039687484928	29.115010803839176	21.31738326738101
80-84	20.765002741847457	29.302717900168673	28.83272962419919	21.099549733784684
85-89	21.11841196094228	29.23274688339457	28.750616053893197	20.898225101769956
90-94	21.6898135268077	28.730165870086026	28.84100766974292	20.739012933363355
95-99	22.377278275499	28.244278481035696	28.424191344395915	20.95425189906939
100-101	23.006423281372484	27.632037367091634	28.48496489360181	20.876574457934073
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.45372384175501
2	36.1446621314544
3	38.608099427599534
4	36.91143829391059
5	21.744478933276465
6	36.3319350070783
7	38.561068967736105
8	37.18923282619138
9	60.02705174344651
10-14	41.1557474849139
15-19	39.717702141093795
20-24	39.62604094938328
25-29	39.68244043158171
30-34	39.686010980476986
35-39	39.65572357317087
40-44	39.52569318984337
45-49	39.53489913996122
50-54	39.476441219886496
55-59	39.56527521229701
60-64	40.084865864793144
65-69	40.921953257216366
70-74	41.44070034445408
75-79	41.594592321311545
80-84	41.86455247563214
85-89	42.016637062712235
90-94	42.42882646017105
95-99	43.331530174568385
100-101	43.88299773930655
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	5.0
2	6.5
3	9.0
4	10.5
5	20.0
6	24.5
7	24.0
8	33.0
9	47.0
10	51.5
11	60.0
12	98.0
13	153.5
14	212.0
15	281.0
16	427.0
17	632.5
18	903.5
19	1280.5
20	1797.5
21	2674.0
22	3841.5
23	5431.0
24	7456.5
25	9900.0
26	12871.5
27	16268.5
28	20425.0
29	24879.5
30	29640.5
31	34762.5
32	39961.5
33	45346.5
34	51483.5
35	58519.5
36	66366.5
37	73389.0
38	78323.5
39	80890.0
40	80906.0
41	79246.0
42	75984.0
43	71923.5
44	67989.5
45	63585.0
46	58839.0
47	53208.5
48	46879.0
49	40726.5
50	34829.0
51	30426.0
52	26423.5
53	22785.0
54	19673.0
55	16772.5
56	14433.5
57	12156.5
58	9973.5
59	8194.5
60	6750.5
61	5484.5
62	4452.0
63	3305.0
64	2471.0
65	2131.0
66	1737.0
67	1362.0
68	1139.0
69	1103.5
70	1065.0
71	886.5
72	727.0
73	581.0
74	458.0
75	379.0
76	271.0
77	186.0
78	136.5
79	99.5
80	67.0
81	51.5
82	47.0
83	37.0
84	30.5
85	20.0
86	11.5
87	12.5
88	9.5
89	7.5
90	10.0
91	8.0
92	6.5
93	8.5
94	7.5
95	4.5
96	2.0
97	4.0
98	4.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03548816703617258
2	0.03262831474754329
3	0.029703465815990603
4	0.027753566528288816
5	0.033993244248934545
6	0.0022748825023187554
7	6.499664292339301E-4
8	0.00324983214616965
9	5.19973143387144E-4
10-14	0.006798648849786909
15-19	0.025517682011724097
20-24	0.021435892836135013
25-29	0.0021318898878872907
30-34	0.00185890398760904
35-39	0.005927693834613442
40-44	0.013220317170618136
45-49	0.007929590436653946
50-54	0.004549765004637511
55-59	0.003873799918234223
60-64	0.010425461524912237
65-69	0.014585246672009392
70-74	0.018251057332888756
75-79	0.006915642807049016
80-84	0.010542455482174345
85-89	0.007825595807976518
90-94	0.008592556194472556
95-99	0.008423564922871734
100-101	0.004874748219254476
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1538541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.356166290258123
#Duplication Level	Relative count
1	100.0
2	6.859112399696834
3	2.7026214164551328
4	1.8484655269901231
5	1.4316133922019176
6	1.1513058960817102
7	0.8764120640496613
8	0.7518977900220156
9	0.617759224282088
10++	4.064458693743007
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	370335	4.815521	11.190461	75-79
GTCTC	540340	4.716291	7.7338176	55-59
GACGC	345900	4.592648	11.197219	75-79
CTGTC	516730	4.510214	7.6787877	55-59
CGCTG	344655	4.4816	11.068112	75-79
CTGCC	338330	4.2818336	10.72611	80-84
GCCGA	319120	4.2370796	11.337944	80-84
CGACG	311970	4.1421466	11.029569	95-97
TCTCT	699705	4.099523	6.239374	60-64
TGCCG	312450	4.062834	11.023027	80-84
CCGAC	307025	3.967594	10.854539	80-84
CTCTT	654610	3.8353145	6.110938	60-64
GAAGA	556740	3.665907	7.404259	85-89
TGTCT	603605	3.6335437	5.7296925	55-59
ACACA	558550	3.4839566	6.572948	6
CTGAC	388055	3.4585242	7.5127697	70-74
TGACG	373420	3.4194343	7.7526355	75-79
GAGGA	350155	3.3638787	8.902914	90-94
TACAC	518810	3.1692386	7.6662154	5
ATCTG	513695	3.1575265	6.1091213	70-74
AGAGG	328220	3.1531527	8.653176	90-94
CACAT	513335	3.1357934	5.4864497	65-69
CATCT	522645	3.1267219	5.818257	70-74
GACGA	334315	3.1259117	8.253136	85-89
ACGCT	348095	3.1023822	7.5645566	75-79
ACATC	496165	3.0309076	5.690314	70-74
TCTGA	484405	2.97749	5.7561445	70-74
CGAAG	315965	2.9543355	8.230462	85-89
AAGAG	434545	2.8613024	6.358734	85-89
AGAAG	357090	2.3512926	5.069253	5
TATAC	542605	2.2859907	5.718425	5
ACGAA	348580	2.2339442	5.783574	85-89
AGGAT	331400	2.1370635	5.8247166	90-94
GGATA	293605	1.8933389	5.5676336	90-94
GGTGG	135835	1.8645694	7.5700784	95-97
GGGGG	89010	1.8202066	5.104827	95-97
AGAGA	270525	1.7812973	5.1028132	8
CTTTG	293960	1.7695621	7.517098	9
GATAG	266810	1.7205489	5.3620796	95-97
GAGAG	172855	1.6605881	5.5859547	7
TAGTG	262915	1.6604133	5.088979	95-97
CTCGG	124925	1.6244183	7.4737735	95-97
GTGTA	246145	1.5545039	8.324147	1
CGGTG	97885	1.3077472	6.590247	95-97
CGCCG	64855	1.2227787	5.294879	95-97
AAGAC	187685	1.2028166	5.5027666	5
GAGTC	128075	1.1727922	5.6885457	9
GTCTT	188300	1.1335166	5.8113885	1
TATGA	253625	1.0978472	5.201323	4
GTTCT	182190	1.096736	5.0988364	1
TCATA	255595	1.0768198	5.262599	2
GTGTT	173660	1.0740796	5.817867	1
GACTT	173850	1.0686029	5.472838	7
TGGAC	106195	0.9724354	6.290106	5
GTCCA	106125	0.9458347	6.6237755	1
AGACT	142895	0.8968561	5.092036	6
GGACT	95795	0.8772018	5.6485057	6
GTATA	156515	0.6774945	6.8372827	1
>>END_MODULE