##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-163_GCTACGCT-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1538541	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.491372670601564	34.0	31.0	34.0	31.0	34.0
2	32.67975309075286	34.0	31.0	34.0	31.0	34.0
3	32.7914628209453	34.0	31.0	34.0	31.0	34.0
4	36.293241454078895	37.0	37.0	37.0	35.0	37.0
5	36.02484171692532	37.0	35.0	37.0	35.0	37.0
6	36.11105911379677	37.0	37.0	37.0	35.0	37.0
7	36.10904031806757	37.0	36.0	37.0	35.0	37.0
8	36.13244950898286	37.0	37.0	37.0	35.0	37.0
9	38.01309552361621	39.0	38.0	39.0	35.0	39.0
10-14	38.20527265766724	39.4	38.2	39.4	35.2	39.4
15-19	39.34607150540675	41.0	39.0	41.0	36.0	41.0
20-24	39.277846479229346	40.4	39.0	41.0	36.0	41.0
25-29	39.08489692507382	40.0	39.0	41.0	36.0	41.0
30-34	38.88283432160729	40.0	38.2	41.0	35.2	41.0
35-39	38.6541558528502	40.0	38.0	41.0	34.8	41.0
40-44	38.41126248829248	40.0	38.0	41.0	34.0	41.0
45-49	38.24097076386005	40.0	38.0	41.0	33.6	41.0
50-54	38.197729277282825	40.0	37.6	41.0	33.8	41.0
55-59	37.81850233435443	39.8	36.6	41.0	33.0	41.0
60-64	37.25113883867898	39.0	35.6	40.8	32.4	41.0
65-69	36.494204314347165	37.6	35.0	40.0	31.0	41.0
70-74	35.8886637405178	36.6	35.0	39.2	31.0	41.0
75-79	34.65111323000166	35.2	33.8	37.4	30.2	39.2
80-84	34.42215696559273	35.0	34.0	36.4	31.0	38.2
85-89	33.79825211027851	35.0	34.0	35.4	30.0	36.6
90-94	33.391176575729865	35.0	34.0	35.0	29.6	36.0
95-99	33.11721975559963	35.0	33.6	35.0	29.0	35.4
100-101	32.74089380783482	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	12.0
15	67.0
16	154.0
17	303.0
18	529.0
19	827.0
20	1260.0
21	1886.0
22	2483.0
23	3461.0
24	4695.0
25	5957.0
26	7786.0
27	10121.0
28	12798.0
29	15884.0
30	19620.0
31	25558.0
32	32195.0
33	43768.0
34	63144.0
35	100853.0
36	187664.0
37	376899.0
38	508509.0
39	112000.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.65981992030112	23.535934368989842	11.349973773854579	24.45427193685446
2	15.274039924176828	23.964062000265223	39.69750038353628	21.064397692021668
3	15.667245786755114	28.067565310251723	33.29401036436468	22.971178538628482
4	11.28348220814395	18.569085906712917	44.24028998902207	25.907141896121068
5	10.745310004738256	39.42514369132834	38.983101522806344	10.846444781127055
6	25.260230309104536	44.138765232775725	19.589273214038492	11.011731244081243
7	23.211471127516266	34.112253102127276	27.45328203798274	15.222993732373723
8	20.96512215144088	38.37167810282599	24.53525775393701	16.127941991796124
9	23.095972093041397	14.933498684792932	24.919582903543034	37.05094631862264
10-14	20.375862586697398	25.943072040329117	32.965556329015605	20.71550904395788
15-19	19.73021186905023	31.58254479710382	28.777976943903056	19.909266389942893
20-24	19.755020765270547	30.13974894483723	30.209113840064518	19.896116449827705
25-29	19.718892738404787	30.436276108886158	29.884015690977556	19.960815461731496
30-34	19.595710858109182	30.32085362670186	29.991635648103372	20.091799867085587
35-39	19.693440440044565	30.40220417631902	29.93890422962615	19.965451154010267
40-44	19.410823450579816	29.882668544393088	30.552492992666803	20.154015012360297
45-49	19.31209905111908	29.627582837840155	30.773598854205087	20.286719256835674
50-54	18.787993320576064	29.401912186046854	31.102579673226387	20.707514820150696
55-59	18.70485633682074	29.28012418006474	31.02414350829231	20.99087597482222
60-64	18.43931486887292	28.882606549044475	30.781567760567242	21.89651082151536
65-69	18.81097650186915	28.98444403839864	29.821395478282582	22.38318398144963
70-74	18.849332477431837	28.789866563084903	29.36022923184401	23.000571727639247
75-79	19.175552458213836	28.832953878640538	28.793864455507865	23.197629207637764
80-84	18.977809467293323	28.519602038037384	28.68366677737776	23.818921717291534
85-89	19.069109414996433	28.17970478452796	28.769462343953183	23.981723456522424
90-94	19.07857551045665	27.454174988599323	29.112031369677883	24.35521813126615
95-99	19.14796092535149	26.960784696065993	29.237907598486746	24.653346780095774
100-101	19.1995270842724	26.10928994069379	29.954005110687692	24.737177864346112
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.11409185715557
2	36.33843761619849
3	38.63842432538359
4	37.19062410426501
5	21.591754785865312
6	36.271961553185776
7	38.434464859889985
8	37.093064143237
9	60.14691841166404
10-14	41.091371630655274
15-19	39.63947825899312
20-24	39.65113721509825
25-29	39.67970820013628
30-34	39.68751072519477
35-39	39.65889159405483
40-44	39.56483846294011
45-49	39.59881830795475
50-54	39.49550814072676
55-59	39.69573231164296
60-64	40.33582569038828
65-69	41.194160483318775
70-74	41.849904205071084
75-79	42.373181665851604
80-84	42.79673118458486
85-89	43.05083287151886
90-94	43.433793641722794
95-99	43.801307705447265
100-101	43.93670494861851
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	3.5
8	8.5
9	13.0
10	20.5
11	35.0
12	63.0
13	97.5
14	167.0
15	254.5
16	365.0
17	573.0
18	896.0
19	1295.0
20	1833.5
21	2712.5
22	3863.0
23	5482.0
24	7453.0
25	9735.0
26	12763.5
27	16164.0
28	20059.0
29	24447.5
30	29066.0
31	34169.0
32	39420.5
33	44518.5
34	50339.5
35	57015.0
36	64441.0
37	71304.5
38	76119.0
39	78435.5
40	77908.5
41	75752.0
42	72247.5
43	68579.5
44	65984.5
45	62991.5
46	59604.0
47	55593.0
48	50434.5
49	44522.0
50	38691.0
51	33883.0
52	29491.5
53	25536.0
54	21737.0
55	18414.0
56	15831.5
57	13254.5
58	10725.5
59	8666.0
60	7016.5
61	5645.5
62	4574.5
63	3422.5
64	2547.5
65	2156.0
66	1799.5
67	1447.5
68	1175.5
69	1076.0
70	1023.5
71	850.0
72	705.0
73	588.0
74	448.5
75	329.0
76	241.5
77	178.5
78	112.5
79	69.5
80	49.5
81	28.5
82	20.5
83	19.0
84	12.0
85	6.5
86	6.0
87	3.0
88	0.0
89	1.5
90	2.5
91	1.0
92	0.5
93	1.0
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014624244657763427
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.879489724355737E-4
20-24	6.889644149879659E-4
25-29	0.018277055990058112
30-34	0.0069806394499724094
35-39	0.04292378298660874
40-44	0.051659331795512756
45-49	0.052894268011057226
50-54	0.05223130225323862
55-59	0.04193583401417317
60-64	0.053726225040476666
65-69	0.043807737330366886
70-74	0.003002844903060757
75-79	0.0011309415868670384
80-84	1.29993285846786E-4
85-89	0.0028858509457986496
90-94	0.002352878473826827
95-99	0.0019109013019477546
100-101	3.24983214616965E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1538541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.85540777085861
#Duplication Level	Relative count
1	100.0
2	6.936402224382468
3	2.7798742897855924
4	1.9412023982755602
5	1.417711735972322
6	1.149626552509072
7	0.9141463237913524
8	0.7511215500637004
9	0.6182865492485765
10++	4.250720026083964
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	380935	4.9971933	11.461645	75-79
GAGCC	369800	4.851122	11.314081	75-79
CGAGC	359630	4.7177095	11.244116	75-79
CTCCG	394310	4.5033326	9.718653	70-74
GTCTC	540110	4.4611893	7.3656974	55-59
CTGTC	517885	4.2776165	7.2727356	55-59
AGCCC	352095	4.137047	9.836469	75-79
GCCCA	347675	4.085112	9.981296	80-84
TCTCT	702175	3.7570012	5.717496	60-64
CCACG	314380	3.6939025	9.8901415	80-84
ACGCT	426000	3.6200318	13.513362	90-94
TGTCT	604065	3.6084805	5.7362347	55-59
TCTCC	485625	3.5927312	6.9778347	70-74
ATCTC	652295	3.5906603	8.865671	95-97
CCCAC	335665	3.5325787	8.996226	80-84
CTCTT	655155	3.5054197	5.522186	60-64
TCCGA	408580	3.4720013	7.4493456	75-79
CGCTA	398195	3.383752	13.415295	90-94
CGAGA	342305	3.3411431	8.632897	85-89
GAGAC	341925	3.337434	8.811803	85-89
GACGC	253890	3.3305876	10.914199	85-89
ACACA	560320	3.264638	6.0537534	6
ACGAG	328995	3.211228	8.293489	80-84
TACAC	519395	2.941456	7.0814066	5
CACAT	515695	2.920502	5.128378	65-69
CATCT	521790	2.8722749	5.343515	70-74
AGACG	291255	2.8428586	8.403823	85-89
CACGA	322075	2.8157501	7.4783735	80-84
ACATC	496995	2.8145995	5.181663	70-74
AGAAG	358135	2.6009688	5.711404	5
GCTAC	283565	2.409658	7.674713	90-94
TATAC	545225	2.2331228	5.8780355	5
CTACG	234485	1.9925894	7.200039	90-94
CAAAG	298035	1.9387014	5.0241556	4
AGAGA	266590	1.93612	5.686756	8
TACGC	221080	1.8786775	7.0978527	90-94
GAGAG	168785	1.8393333	6.6943254	7
TCTCG	221700	1.8311932	6.644382	90-94
CTTTG	293420	1.7527921	7.640453	9
AAGAG	230465	1.6737605	5.123361	7
CTCGT	193110	1.5950462	6.5666847	90-94
AGGAG	139480	1.5199822	5.531933	7
GTATG	219280	1.5045921	5.7704377	90-94
GCTAT	243705	1.4977506	5.3816824	95-97
TGCCG	109475	1.3959051	9.894109	95-97
GCCGT	107625	1.372316	9.712754	95-97
GTCTT	223775	1.3367563	5.689046	1
ATGCC	154580	1.3135786	6.779254	95-97
CCGTC	109145	1.2465222	8.020288	95-97
AAGAC	189715	1.2340856	5.749551	5
GAGTC	128625	1.2203178	6.0765066	9
GATTG	175495	1.2041608	5.4791975	7
TATGA	253670	1.1599804	5.323256	4
GTGTT	170865	1.1395636	5.8923845	1
GACTT	175785	1.0803311	5.7121654	7
TCATA	255420	1.0461447	5.060501	2
TGGAC	106650	1.011832	6.9596934	5
GTGTA	147080	1.009191	8.790425	1
CGTCT	114730	0.9476446	5.305183	95-97
GAGTA	130260	0.9195284	5.4186645	1
GGACT	95905	0.90988964	6.140905	6
GTCCA	105965	0.90046155	6.271547	1
AGACT	141845	0.8968574	5.0030303	6
GAATA	188685	0.88767356	5.0005035	1
GGATA	121175	0.85539573	5.1619363	1
GTATA	156340	0.71491045	7.060117	1
>>END_MODULE