##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-162_GCTACGCT-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2254610	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.746549070570964	33.0	31.0	34.0	30.0	34.0
2	31.943983216609524	33.0	31.0	34.0	30.0	34.0
3	32.09614833607586	34.0	31.0	34.0	30.0	34.0
4	35.623631581515205	37.0	35.0	37.0	33.0	37.0
5	35.477043923339295	37.0	35.0	37.0	33.0	37.0
6	35.603771383964414	37.0	35.0	37.0	33.0	37.0
7	35.62252274229246	37.0	35.0	37.0	33.0	37.0
8	35.67781124008143	37.0	35.0	37.0	33.0	37.0
9	37.51025410159629	39.0	38.0	39.0	35.0	39.0
10-14	37.63446281175014	39.2	37.2	39.4	34.6	39.4
15-19	38.68302500210679	40.0	38.0	41.0	34.6	41.0
20-24	38.554306243651894	40.0	38.0	41.0	34.0	41.0
25-29	38.34128430194136	40.0	38.0	41.0	33.8	41.0
30-34	38.028930236271464	40.0	38.0	41.0	33.0	41.0
35-39	37.6441475909359	40.0	37.4	41.0	32.0	41.0
40-44	37.55318738052257	40.0	37.0	41.0	31.8	41.0
45-49	37.34641946944261	40.0	36.4	41.0	31.4	41.0
50-54	36.55893418373909	38.6	35.2	40.2	30.4	40.6
55-59	37.15163908613907	39.6	35.2	41.0	31.2	41.0
60-64	36.69110728684784	38.8	35.0	41.0	31.0	41.0
65-69	36.02128855988397	37.4	35.0	40.0	30.2	41.0
70-74	35.170958702391985	36.2	34.2	39.2	29.0	40.8
75-79	34.18308621003189	35.0	33.8	37.2	28.4	39.4
80-84	33.1551834685378	35.0	33.0	36.2	26.4	37.8
85-89	32.20814349266614	35.0	32.4	35.0	24.6	36.4
90-94	31.41326562021813	35.0	31.4	35.0	21.2	35.8
95-99	30.656981828342815	34.2	30.4	35.0	18.4	35.0
100-101	29.52833549926595	33.5	28.0	35.0	13.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	7.0
6	55.0
7	237.0
8	464.0
9	750.0
10	952.0
11	1291.0
12	1801.0
13	2222.0
14	2802.0
15	3307.0
16	3749.0
17	4487.0
18	5097.0
19	6054.0
20	7014.0
21	8175.0
22	9519.0
23	11028.0
24	12573.0
25	14778.0
26	17754.0
27	21596.0
28	26302.0
29	31975.0
30	40178.0
31	51220.0
32	67012.0
33	90987.0
34	128171.0
35	193106.0
36	304118.0
37	491081.0
38	577970.0
39	116710.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57720418428643	22.142891686754417	10.311638561561693	24.968265567397456
2	16.100283420856012	23.44979262537868	37.99157191205257	22.458352041712732
3	16.664721971432943	27.16922619615394	31.866752144525606	24.29929968788751
4	11.943464339792484	18.274977362577204	42.49566661387189	27.28589168375842
5	12.06377292055627	38.017820000425935	37.43748535852968	12.48092172048812
6	26.52451397627309	42.07935789114647	19.234169161228458	12.161958971351979
7	24.591168077268012	32.925218465054016	26.000778854197254	16.482834603480715
8	22.205653141624833	36.73677132391882	23.871806768050515	17.18576876640583
9	23.625797758270984	14.572391808920345	23.75837122255443	38.04343921025424
10-14	21.658112042423415	24.8800019445957	31.67121854647504	21.790667466505848
15-19	21.19894534093462	30.11056777895175	27.502925369277964	21.187561510835668
20-24	21.063597498137888	28.712793806653565	28.993616693749914	21.229992001458637
25-29	21.103608001095026	29.224770724807055	28.426477983700067	21.24514329039786
30-34	20.90185991697726	28.992492114879916	28.552140703098562	21.553507265044267
35-39	21.07121024441182	29.188170869243656	28.46404881157169	21.276570074772838
40-44	20.706323501987377	28.660520737277313	29.11853773818419	21.514618022551122
45-49	20.629390202390553	28.21866557985514	29.528880865945588	21.623063351808717
50-54	20.029127605418793	28.066560047010114	29.931546490085402	21.97276585748569
55-59	20.025491986648642	28.13843028763115	29.90289164078228	21.933186084937923
60-64	19.917263356432052	27.477676808988004	29.970670454253096	22.634389380326848
65-69	20.47944217411139	27.4707105743224	29.161669538518208	22.888177713047998
70-74	20.560066903958287	27.547018112369337	28.788141191768457	23.104773791903916
75-79	21.194982189761543	28.239909128394817	27.999130247171617	22.56597843467202
80-84	21.813065768522538	28.31823493528472	27.635444660479447	22.233254635713294
85-89	22.275801338749464	28.20019176700664	27.511477099208197	22.012529795035704
90-94	22.7431472596402	27.704718116195703	27.76131897386726	21.790815650296835
95-99	23.583488976987155	27.162708972287014	27.246376400160788	22.00742565056504
100-101	24.18818304745983	26.42297095385623	27.511433424655834	21.877412574028114
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	67.54546975168388
2	38.558635462568745
3	40.96402165932045
4	39.229356023550906
5	24.54469464104439
6	38.68647294762507
7	41.07400268074873
8	39.39142190803066
9	61.66923696852522
10-14	43.448779508929256
15-19	42.38650685177029
20-24	42.29358949959652
25-29	42.34875129149288
30-34	42.455367182021526
35-39	42.34778031918466
40-44	42.2209415245385
45-49	42.25245355419927
50-54	42.00189346290449
55-59	41.95867807158656
60-64	42.551652736758896
65-69	43.36761988715939
70-74	43.6648406958622
75-79	43.76096062443356
80-84	44.046320404235836
85-89	44.288331133785164
90-94	44.53396290993704
95-99	45.590914627552195
100-101	46.065595621487944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	3.0
2	4.5
3	8.5
4	10.0
5	11.0
6	19.0
7	25.0
8	35.5
9	45.0
10	48.5
11	67.0
12	91.0
13	122.0
14	182.5
15	249.0
16	364.5
17	528.5
18	736.5
19	1004.5
20	1449.0
21	2190.0
22	3160.0
23	4657.5
24	6784.5
25	9455.5
26	13013.5
27	17265.0
28	22328.0
29	27969.0
30	34199.0
31	41119.5
32	48230.5
33	56205.5
34	65679.0
35	77243.5
36	90953.5
37	103817.5
38	113319.5
39	117240.0
40	115309.5
41	110276.5
42	103130.0
43	96126.5
44	90390.5
45	83902.0
46	76765.5
47	69792.0
48	62683.5
49	55419.5
50	49110.0
51	44204.0
52	39989.0
53	36597.5
54	33835.0
55	31177.5
56	28812.0
57	26790.0
58	24761.0
59	22875.5
60	20875.0
61	19128.5
62	17841.5
63	16376.0
64	14944.5
65	13806.0
66	12729.5
67	11599.0
68	10576.5
69	9747.5
70	8812.0
71	7590.5
72	6349.5
73	5267.0
74	4207.0
75	3269.5
76	2512.5
77	1891.0
78	1403.0
79	1020.0
80	724.5
81	525.0
82	396.0
83	282.0
84	209.5
85	156.0
86	119.0
87	88.0
88	67.0
89	57.5
90	47.5
91	30.5
92	27.0
93	31.0
94	29.5
95	24.0
96	19.0
97	21.0
98	16.5
99	7.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03322082311353183
2	0.03184586247732424
3	0.027277444879602236
4	0.02656778777704348
5	0.032732933855522686
6	0.001729789187486971
7	7.096571025587574E-4
8	0.0033708712371540977
9	4.878892580091457E-4
10-14	0.006954639605075823
15-19	0.023755771508154402
20-24	0.019914752440555128
25-29	0.0021555834490222256
30-34	0.0020846177387663497
35-39	0.005686127534252044
40-44	0.012560930715290006
45-49	0.008276375958591507
50-54	0.00516275542111496
55-59	0.00432890832560842
60-64	0.010112613711462293
65-69	0.01398024492040752
70-74	0.01745756472294543
75-79	0.007823969555710299
80-84	0.010769046531329143
85-89	0.009207800905699877
90-94	0.010077130856334356
95-99	0.00923441304704583
100-101	0.0064534442763937
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2254610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.66292765002805
#Duplication Level	Relative count
1	100.0
2	6.849189354542673
3	2.650963597430407
4	1.8470480269195473
5	1.4732334047109208
6	1.1985316610584276
7	0.9813398592841848
8	0.7965738758029979
9	0.7084735393086571
10++	5.856225145304374
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	633990	4.6524706	10.155498	75-79
GTCTC	820660	4.4626803	7.3928747	55-59
CTGTC	803350	4.3685503	7.300042	55-59
CGCTG	594580	4.363264	10.04659	75-79
GACGC	565240	4.235147	9.830426	75-79
GCCGA	543620	4.0731554	10.123642	80-84
TCTCT	998815	4.0248427	6.348052	60-64
CTGCC	560925	3.9981122	9.565556	80-84
TGCCG	538500	3.951727	9.764289	80-84
CGACG	518095	3.8819056	9.883036	80-84
CTCTT	949095	3.82449	6.138939	60-64
TGTCT	914130	3.7924757	5.9806566	55-59
ACACA	860320	3.690005	6.6008306	70-74
CCGAC	496590	3.6139536	9.5781145	80-84
TACAC	813670	3.4180696	8.0977125	5
CTGAC	607385	3.3723369	7.2671986	70-74
TGACG	588905	3.36638	7.551452	75-79
CACAT	789565	3.3168092	5.9136233	60-64
ATCTG	771835	3.2694426	6.3328595	70-74
ACATC	762785	3.2043116	5.9466004	70-74
CATCT	778525	3.203102	5.9538846	70-74
GACGA	529620	3.0911257	8.0208435	85-89
ACGCT	551185	3.0603023	7.284565	75-79
TCTTA	966925	3.0351021	4.722024	60-64
TCTGA	710550	3.009843	5.9478416	70-74
GAGAG	489105	2.9390395	8.212436	90-94
ATACA	890205	2.9129922	5.2493424	6
AGAGA	613525	2.7893388	6.6677375	90-94
AGAGG	435295	2.6156943	8.058528	95-97
TATAC	814170	2.609336	5.5327973	5
ACGAA	525695	2.32141	6.0381503	85-89
CGAAT	526725	2.2780724	5.8773417	85-89
GAGGT	352215	2.0728924	6.705161	95-97
TAGAG	461735	2.0560205	6.0044	90-94
AGGTG	346595	2.039817	6.994415	95-97
GGTGT	338450	1.9508735	5.936583	85-89
CTTTG	464185	1.9257767	8.920992	9
GGTGG	239255	1.8610767	7.799576	95-97
GTGTA	385485	1.681155	9.058525	1
GCTTT	397795	1.6503428	5.3470836	1
CTCGG	203900	1.4962991	6.6641016	95-97
CGGTG	181115	1.3683797	6.356609	95-97
AAGAC	292030	1.2895715	5.661311	5
TATGA	387510	1.2786418	6.013247	4
TCATA	388890	1.2463548	6.0924244	2
GAGTC	207410	1.1856257	6.877765	9
GTCTT	285435	1.184192	6.210425	1
GACTT	278685	1.1804914	6.5200963	7
ATGAG	256665	1.1428818	5.361782	7
TGAGT	258425	1.1270282	5.7929626	8
GTGTT	263355	1.1248834	5.604589	1
GATTG	253350	1.1048955	5.410017	7
TGGAC	179020	1.0233388	6.8987465	5
GTCCA	176225	0.97844046	7.4791684	1
GGACT	157880	0.90249544	6.4728413	6
AGACT	207515	0.89749724	5.2497015	6
TATAA	334625	0.8353914	5.2350016	2
GTATA	212810	0.70219547	6.860072	1
>>END_MODULE