##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-162_GCTACGCT-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2254610	
Filtered Sequences	0	
Sequence length	101	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.464921205884835	34.0	31.0	34.0	31.0	34.0
2	32.65125365362524	34.0	31.0	34.0	31.0	34.0
3	32.76686566634584	34.0	31.0	34.0	31.0	34.0
4	36.27654272801061	37.0	37.0	37.0	35.0	37.0
5	35.98980488865037	37.0	35.0	37.0	35.0	37.0
6	36.070035615915835	37.0	36.0	37.0	35.0	37.0
7	36.074861727748925	37.0	36.0	37.0	35.0	37.0
8	36.102815121018715	37.0	36.0	37.0	35.0	37.0
9	37.9860153197227	39.0	38.0	39.0	35.0	39.0
10-14	38.17410656388466	39.4	38.2	39.4	35.2	39.4
15-19	39.29648764087802	40.8	39.0	41.0	36.0	41.0
20-24	39.20897334794044	40.0	39.0	41.0	36.0	41.0
25-29	38.98686256159602	40.0	38.8	41.0	35.6	41.0
30-34	38.7379272690177	40.0	38.0	41.0	34.8	41.0
35-39	38.436494471327634	40.0	38.0	41.0	34.0	41.0
40-44	38.10497993001007	40.0	37.6	41.0	33.2	41.0
45-49	37.859965404216254	40.0	37.0	41.0	33.0	41.0
50-54	37.783346831602806	40.0	36.4	41.0	33.0	41.0
55-59	37.37461175103455	39.2	35.4	41.0	32.6	41.0
60-64	36.801027938313055	38.6	35.0	40.4	31.4	41.0
65-69	36.05692487836034	37.2	35.0	39.8	30.8	41.0
70-74	35.49436807252695	36.2	34.6	39.0	30.6	40.8
75-79	34.3048085478198	35.0	33.4	37.0	29.4	39.2
80-84	34.13624715582739	35.0	34.0	36.2	30.0	37.8
85-89	33.55411995866247	35.0	34.0	35.2	29.4	36.6
90-94	33.16271426100302	35.0	33.4	35.0	29.0	36.0
95-99	32.89696204665109	35.0	33.0	35.0	29.0	35.0
100-101	32.49730707306364	35.0	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	21.0
14	69.0
15	155.0
16	344.0
17	664.0
18	1083.0
19	1622.0
20	2403.0
21	3344.0
22	4536.0
23	6214.0
24	8119.0
25	10404.0
26	13261.0
27	16758.0
28	20917.0
29	26068.0
30	32462.0
31	41185.0
32	53771.0
33	72751.0
34	107116.0
35	176029.0
36	306068.0
37	526314.0
38	682979.0
39	139854.0
40	94.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.132164764637785	22.207344063940106	10.626006271594644	25.034484899827465
2	16.28607853905261	23.262201694703233	37.904723291035246	22.54699647520891
3	16.80760752413943	26.914100443092153	32.011922239322985	24.26636979344543
4	12.146535320964603	18.25344516346508	42.21554947418844	27.38447004138188
5	11.935190565108822	38.03389499736096	37.556118353063276	12.474796084466936
6	26.53864748226966	42.25032267221382	19.143665645056128	12.06736420046039
7	24.453852329227672	32.923121959008434	26.114627363490804	16.508398348273094
8	22.23275865892549	36.666163992885686	23.920988552343864	17.18008879584496
9	23.588514199795085	14.430389291274324	23.79338333459002	38.18771317434057
10-14	21.64571256226132	24.80787364555289	31.713759807682923	21.832653984502862
15-19	21.150206734185346	30.11024640443966	27.549661002064234	21.189885859310756
20-24	21.029068961852698	28.661226049602117	29.032254630671943	21.27745035787324
25-29	21.07969101493688	29.17575444089505	28.48526252329521	21.25929202087286
30-34	20.887519277350417	28.961003153217412	28.584238738940144	21.56723883049203
35-39	21.043193798936677	29.12659726287845	28.498594414879104	21.33161452330577
40-44	20.711296978292502	28.58923229645421	29.175477188200727	21.52399353705256
45-49	20.60677525526225	28.174860276291362	29.55353912885112	21.664825339595264
50-54	20.003486217459383	27.995630867793015	29.947664136673925	22.05321877807368
55-59	19.844114602071407	27.963154618656016	29.91292902074301	22.279801758529562
60-64	19.575773481489225	27.425688557549783	29.75754008936251	23.24099787159848
65-69	19.980357193093123	27.56361304469098	28.737664341223617	23.718365420992278
70-74	20.043680867088277	27.440428931211507	28.1591850023109	24.35670519938932
75-79	20.26446872773414	27.653442816306484	27.521666188823	24.560422267136374
80-84	20.04194792063817	27.351449380139815	27.437335813826742	25.16926688539528
85-89	20.05466128067323	27.00453378677115	27.633052465887488	25.30775246666813
90-94	20.088629660020846	26.244030959615	28.08186112527999	25.585478255084166
95-99	20.115554110978856	25.698312772658184	28.20543300013643	25.980700116226522
100-101	20.085043608467636	24.84538332162398	29.06099419499975	26.008578874908633
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	67.16664966446525
2	38.83307501426152
3	41.073977317584855
4	39.531005362346484
5	24.409986649575757
6	38.60601168273005
7	40.96225067750077
8	39.41284745477045
9	61.77622737413566
10-14	43.478366546764185
15-19	42.340092593496095
20-24	42.306519319725936
25-29	42.33898303580974
30-34	42.45475810784245
35-39	42.374808322242444
40-44	42.23529051534506
45-49	42.27160059485752
50-54	42.05670499553306
55-59	42.12391636060097
60-64	42.81677135308771
65-69	43.698722614085405
70-74	44.4003860664776
75-79	44.824890994870515
80-84	45.211214806033446
85-89	45.362413747341364
90-94	45.67410791510501
95-99	46.09625422720538
100-101	46.093622483376265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	1.0
6	1.0
7	2.0
8	4.5
9	6.5
10	9.0
11	22.0
12	45.0
13	72.0
14	101.5
15	162.0
16	259.5
17	399.0
18	610.0
19	913.5
20	1378.5
21	2072.5
22	3079.0
23	4623.0
24	6753.0
25	9461.0
26	12849.0
27	16995.5
28	21996.0
29	27529.5
30	33603.5
31	40207.5
32	47280.5
33	54779.5
34	63537.0
35	74254.5
36	87263.5
37	100068.5
38	109167.0
39	112503.5
40	110020.0
41	104778.5
42	97519.5
43	90633.0
44	86789.0
45	83462.5
46	79500.0
47	74860.5
48	68880.0
49	61510.0
50	54210.5
51	48561.5
52	43913.0
53	39817.0
54	36160.0
55	32906.5
56	30459.0
57	28478.5
58	26187.0
59	24162.0
60	22200.5
61	20423.0
62	19029.0
63	17235.5
64	15752.5
65	14562.5
66	13263.0
67	12097.5
68	11051.0
69	10078.0
70	8946.5
71	7792.0
72	6511.0
73	5223.0
74	4108.5
75	3200.0
76	2453.5
77	1758.0
78	1259.0
79	929.0
80	660.5
81	430.5
82	271.5
83	184.5
84	123.5
85	83.5
86	58.5
87	39.5
88	20.5
89	11.0
90	8.5
91	6.0
92	6.5
93	6.5
94	2.5
95	0.5
96	1.5
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012951242121697322
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010644856538381362
20-24	5.677256820470058E-4
25-29	0.017342245443779634
30-34	0.006688518191616289
35-39	0.042481848301923614
40-44	0.050962250677500764
45-49	0.051574329928457695
50-54	0.051663037066277534
55-59	0.04233104616762988
60-64	0.05241704773774622
65-69	0.0426060382948714
70-74	0.0033708712371540977
75-79	0.001649952763449111
80-84	2.128971307676272E-4
85-89	0.0031402326788225014
90-94	0.0026612141345953404
95-99	0.0020934884525483344
100-101	8.870713781984468E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2254610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.953968894267405
#Duplication Level	Relative count
1	100.0
2	6.817693728409
3	2.715787277647218
4	1.8837476146355532
5	1.5051572959938149
6	1.1952900173648395
7	0.9731673344664453
8	0.8093365158645911
9	0.7130015401324146
10++	6.1065329839912135
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	604970	4.4774985	9.953426	75-79
CGAGC	588960	4.359005	9.928305	75-79
GAGCC	577455	4.2738547	9.965628	75-79
GTCTC	820645	4.2055364	6.979087	60-64
CTGTC	806350	4.132279	6.902351	55-59
CTCCG	611870	3.9275157	8.384816	70-74
TGTCT	915560	3.745934	5.8861976	55-59
ATCTC	977555	3.7101483	9.407478	95-97
TCTCT	1003270	3.6817827	5.7985654	60-64
GCCCA	553765	3.6761575	8.814378	80-84
AGCCC	553360	3.6734688	8.66247	75-79
ACGCT	675235	3.5787456	13.153814	90-94
CTCTT	952750	3.496385	5.626499	60-64
ACACA	859120	3.4875746	6.2554817	70-74
CCACG	509865	3.384728	8.682406	80-84
TCTCC	724970	3.332369	6.5268054	70-74
CGCTA	622750	3.3005748	13.010927	90-94
TCCGA	622545	3.2994878	7.1184063	75-79
CGAGA	527890	3.225983	8.297682	85-89
TACAC	816575	3.205206	7.56498	5
GAGAC	516175	3.1543918	8.374728	85-89
CCCAC	528495	3.1468532	7.844828	80-84
GACGC	422505	3.1270397	9.641702	85-89
ACGAG	509475	3.1134472	8.062568	80-84
CACAT	788555	3.0952225	5.4941545	60-64
ACATC	764270	2.9998996	5.5525575	70-74
CATCT	779260	2.957553	5.5021124	70-74
ATACA	889795	2.8838117	5.3254867	6
CACGA	505445	2.770509	7.2577457	80-84
AGACG	450710	2.7543292	8.006693	85-89
TATAC	815910	2.5568748	5.5899477	5
GCTAC	449480	2.3822436	7.360214	90-94
CAAAG	472085	2.1365983	5.0207605	4
CTACG	366335	1.9415752	6.810254	90-94
CTTTG	468590	1.9171952	8.958557	9
TACGC	352715	1.8693892	6.822101	95-97
TCTCG	339850	1.7416198	6.572045	90-94
GCTTT	399845	1.635931	5.061176	1
GTATG	336150	1.5858028	6.358285	90-94
TGCCG	218930	1.5667404	8.997598	95-97
GCTAT	362470	1.5337522	5.4855814	95-97
CTCGT	296085	1.5173384	6.4756308	90-94
TTGAG	317490	1.4977734	5.0092306	9
GAGAG	218175	1.4864718	5.1103754	7
ATGGA	294565	1.4371663	5.322543	4
GCCGT	197100	1.4105173	8.561542	95-97
GTCTT	344625	1.4100032	5.7039905	1
CTATC	365085	1.3856196	5.0073843	95-97
TATGA	390860	1.3655933	6.41006	4
ATGCC	255985	1.3567203	6.7758436	95-97
AAGAC	290745	1.3158758	5.8217115	5
ATGAG	256635	1.252108	5.736519	7
GAGTC	211310	1.2486162	7.1022544	9
TCATA	390270	1.2230164	6.1255846	2
TGAGT	259055	1.2221037	6.212361	8
GATTG	253590	1.1963226	5.782345	7
GTGTT	261110	1.1910497	5.801927	1
CCGTC	184275	1.182838	7.054703	95-97
GACTT	279230	1.1815312	6.583603	7
TCGTA	265500	1.1234342	5.2643833	90-94
GTGTA	236575	1.1160535	9.5714	1
TATGC	262885	1.1123692	5.3788934	95-97
ATGTG	227910	1.0751758	5.1030107	7
TGGAC	176970	1.0457034	7.6351376	5
ACATG	238510	1.0437572	5.045632	8
CGTAT	246135	1.0414933	5.200394	95-97
GTCCA	178570	0.94642085	6.872343	1
GGACT	157565	0.9310406	7.082199	6
AGACT	206905	0.9054488	5.2472024	6
CGTCT	176410	0.9040433	5.1991124	95-97
TATAA	331700	0.85828143	5.341405	2
GTATA	210355	0.73494184	7.1512694	1
GTATT	192765	0.6512062	5.0029597	1
>>END_MODULE