##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-160_CAGAGAGG-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1531303	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.651531408219014	33.0	31.0	34.0	30.0	34.0
2	31.84663192065842	33.0	31.0	34.0	30.0	34.0
3	31.988108819743708	34.0	31.0	34.0	30.0	34.0
4	35.52750174198052	37.0	35.0	37.0	33.0	37.0
5	35.3880570990849	37.0	35.0	37.0	33.0	37.0
6	35.52807184469697	37.0	35.0	37.0	33.0	37.0
7	35.54431618040323	37.0	35.0	37.0	33.0	37.0
8	35.595859865748324	37.0	35.0	37.0	33.0	37.0
9	37.40395924255356	39.0	37.0	39.0	35.0	39.0
10-14	37.523193776803154	39.2	37.2	39.4	34.0	39.4
15-19	38.56823319747953	40.0	38.0	41.0	34.0	41.0
20-24	38.45884948961766	40.0	38.0	41.0	34.0	41.0
25-29	38.27112256685973	40.0	38.0	41.0	33.6	41.0
30-34	38.00357904346821	40.0	38.0	41.0	33.0	41.0
35-39	37.68519359003411	40.0	37.6	41.0	32.2	41.0
40-44	37.63964649713349	40.0	37.2	41.0	31.8	41.0
45-49	37.4791725739452	40.0	37.0	41.0	31.6	41.0
50-54	36.78484532453734	39.0	36.0	40.2	31.0	40.6
55-59	37.417407266883174	40.0	36.4	41.0	31.8	41.0
60-64	36.96551381405247	39.0	35.4	41.0	31.0	41.0
65-69	36.2954671936253	38.0	35.0	40.2	30.4	41.0
70-74	35.45401282437245	36.6	34.6	39.2	29.4	41.0
75-79	34.49114251065922	35.4	34.0	37.6	29.0	39.4
80-84	33.54116761999421	35.0	33.6	36.4	27.6	38.2
85-89	32.7372710691483	35.0	33.0	35.4	26.0	36.6
90-94	32.11752618521612	35.0	32.4	35.0	25.0	36.0
95-99	31.57848237742628	34.8	31.8	35.0	23.6	35.2
100-101	30.65240647997163	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	45.0
7	157.0
8	324.0
9	431.0
10	511.0
11	696.0
12	1056.0
13	1486.0
14	1882.0
15	2138.0
16	2608.0
17	3012.0
18	3322.0
19	4069.0
20	4417.0
21	5163.0
22	6104.0
23	7019.0
24	8338.0
25	9523.0
26	11225.0
27	13226.0
28	16094.0
29	19274.0
30	24342.0
31	30612.0
32	40109.0
33	54206.0
34	76977.0
35	117101.0
36	199714.0
37	355993.0
38	424698.0
39	85382.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.635288491940095	23.492410444816656	10.506484242102761	24.365816821140484
2	15.351088924062417	23.670535391746792	39.595237333120814	21.38313835106998
3	15.896137829672574	27.640924307993792	32.72370376418714	23.739234098146486
4	11.359035644041656	18.39417279002304	43.7245204904085	26.522271075526803
5	11.075530749467438	39.03712224989825	38.45115680814107	11.43619019249324
6	25.785650939933834	43.198426412447645	19.602188498757247	11.413734148861279
7	23.890805933812754	33.7315809133725	26.688247571331917	15.689365581482825
8	21.233949345826865	38.06129126214861	24.339002354944927	16.365757037079607
9	22.947329651054204	15.073747892310967	25.383890007039778	36.59503244959505
10-14	20.535394391928634	25.93280748004082	32.643308170936756	20.88848995709379
15-19	19.863452692472	31.55345424711509	28.62638791088629	19.95670514952662
20-24	19.83926551236731	29.99659040139206	30.203517765184472	19.960626321056164
25-29	19.82799909356345	30.358035906119817	29.7983178679672	20.01564713234953
30-34	19.7180306106176	30.20287831180418	29.840408559521016	20.238682518057203
35-39	19.84691635767461	30.392004990546074	29.781914390609497	19.97916426116982
40-44	19.59695827920583	30.09626368372687	30.178596270541508	20.12818176652579
45-49	19.704948122186963	29.8667924785596	30.240557773025	20.187701626228435
50-54	19.358634108699057	29.645111779072685	30.55005995849861	20.446194153729646
55-59	19.509853003966853	29.61230209322048	30.530134232509553	20.347710670303112
60-64	19.28652152695559	29.12684450704991	30.762827298443096	20.82380666755141
65-69	19.667259369025405	29.213181087378054	30.178469072275377	20.941090471321168
70-74	19.70267716222737	28.908421974211517	30.128911047693247	21.25998981586786
75-79	20.084645574413432	29.1394518988762	29.73687170051214	21.03903082619823
80-84	20.255541551397965	28.893774451670613	29.603510499351916	21.247173497579507
85-89	20.55238499783695	28.765744810247213	29.49970584608697	21.182164345828866
90-94	20.734816393866602	28.250396105431314	29.780545182143186	21.234242318558895
95-99	21.231859041452303	28.002441544076778	29.331980635363124	21.433718779107792
100-101	21.71117165856634	27.36746790455155	29.59174876927719	21.32961166760492
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.00110531308059
2	36.7342272751324
3	39.63537192781906
4	37.88130671956846
5	22.51172094196068
6	37.19938508879511
7	39.58017151529558
8	37.59970638290647
9	59.54236210064925
10-14	41.42388434902242
15-19	39.820157841998615
20-24	39.79989183342347
25-29	39.843646225912984
30-34	39.95671312867481
35-39	39.826080618844436
40-44	39.725140045731614
45-49	39.8926497484154
50-54	39.80482826242871
55-59	39.85756367426997
60-64	40.110328194507
65-69	40.60834984034657
70-74	40.96266697809523
75-79	41.12367640061166
80-84	41.502715048977464
85-89	41.73454934366582
90-94	41.9690587124255
95-99	42.665577820560095
100-101	43.04078332617126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6.0
1	3.5
2	1.0
3	3.5
4	8.5
5	16.5
6	19.5
7	21.5
8	31.0
9	36.0
10	43.5
11	57.0
12	85.5
13	125.5
14	182.0
15	237.5
16	350.5
17	507.0
18	709.0
19	978.5
20	1376.5
21	2039.5
22	3014.0
23	4349.5
24	6108.0
25	8362.5
26	11283.5
27	14936.0
28	19293.5
29	24046.5
30	29249.5
31	34963.0
32	40702.5
33	47154.5
34	54401.5
35	62125.5
36	70468.5
37	78086.5
38	83266.0
39	84949.0
40	82991.0
41	79196.0
42	74690.0
43	69725.0
44	65022.0
45	60186.0
46	55227.5
47	50119.0
48	44050.0
49	37783.0
50	32748.5
51	28930.0
52	25398.0
53	21916.0
54	18870.0
55	16274.0
56	14132.0
57	12103.5
58	10070.0
59	8397.5
60	6887.0
61	5660.5
62	4695.0
63	3619.0
64	2733.5
65	2303.0
66	2028.5
67	1702.5
68	1481.0
69	1443.0
70	1389.5
71	1186.5
72	1020.5
73	869.5
74	668.5
75	525.5
76	411.5
77	304.5
78	222.0
79	171.5
80	129.0
81	87.0
82	72.5
83	54.0
84	39.0
85	31.0
86	28.5
87	25.5
88	17.0
89	12.0
90	7.5
91	9.0
92	9.5
93	6.5
94	6.5
95	4.5
96	2.5
97	3.0
98	4.0
99	3.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03363148899989094
2	0.03134585382514107
3	0.027950052994084124
4	0.027166406648455595
5	0.03239071561931244
6	0.0016325965533927642
7	7.183424834928163E-4
8	0.003722320141735502
9	3.2651931067855287E-4
10-14	0.006713237027551047
15-19	0.02644806416496278
20-24	0.0216155783669202
25-29	0.002089723588342738
30-34	0.0019852374089256012
35-39	0.005733679095515388
40-44	0.012799556978599271
45-49	0.008371955125798095
50-54	0.0050414581568768565
55-59	0.004205568721539761
60-64	0.010592286438412254
65-69	0.015450893781309122
70-74	0.019186274695471763
75-79	0.007731977276868132
80-84	0.011127778107925082
85-89	0.008607049029486653
90-94	0.009469060009678032
95-99	0.009194783788708048
100-101	0.00581204373007824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1531303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.390050220280287
#Duplication Level	Relative count
1	100.0
2	6.503256953195719
3	2.4316217319536393
4	1.6589458239411203
5	1.2714245990190685
6	1.0111049975447128
7	0.783916981712548
8	0.6271335853700384
9	0.5188642965750223
10++	3.5208226099406588
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	410320	3.582811	6.4352117	90-94
CTGTC	384115	3.3539956	5.990557	90-94
TCTCT	573810	3.3517845	5.00244	90-94
GCTGC	256750	3.351235	8.128152	90-94
GACGC	231365	3.0966263	8.493312	95-97
CTCTT	528785	3.0887809	4.9042497	95-97
CGCTG	228605	2.9838717	7.776393	95-97
GCCGA	217745	2.914334	8.23341	90-94
CTGCC	227370	2.8841894	7.7627125	90-94
CGACG	210860	2.822184	8.504131	95-97
ACACA	445940	2.8085015	6.7739754	6
TGCCG	206230	2.6918218	7.8420215	90-94
CCGAC	205830	2.6772938	7.61907	95-97
GAAGG	274755	2.6708403	6.1340475	95-97
TACAC	403305	2.4770515	8.148068	5
CTGAC	271365	2.4296951	6.052355	95-97
TGACG	257805	2.3751614	6.051944	95-97
GACGA	235485	2.2246504	6.269861	95-97
GGCTT	247160	2.220666	5.33968	90-94
CGAAG	222625	2.1031606	5.799431	95-97
ACGCT	233825	2.0935767	5.3984704	85-89
AAGGC	210250	1.9862528	5.1040635	90-94
AGGCT	213640	1.9682685	5.2653456	90-94
CTTTG	317105	1.9059647	7.6135015	9
AGAGA	280715	1.8718518	5.193389	8
TATAC	420445	1.7775477	5.5882325	5
GGTGG	127865	1.7670698	5.30878	95-97
GAGAG	179820	1.7479955	5.680455	7
GTGTA	217015	1.376265	9.471914	1
AAGAC	202675	1.3134158	5.680101	5
CCATG	144380	1.2927216	5.3233852	9
GAGTC	137420	1.2660524	6.3399177	9
GTCTT	202625	1.217881	5.9361567	1
TATGA	270920	1.1785743	5.410405	4
GATTG	184615	1.170791	5.3081956	7
GTGTT	188190	1.1638906	5.6371307	1
GACTT	188780	1.1634951	5.6279817	7
TCATA	271320	1.1470805	5.4037557	2
GTTCT	190545	1.1452739	5.0789714	1
TGGAC	112825	1.0394583	6.533487	5
GTCCA	115225	1.0316793	7.7570252	1
AGACT	152285	0.9624151	5.369369	6
GGACT	102050	0.94018817	5.8750477	6
GTATA	166720	0.72527647	6.809741	1
>>END_MODULE