##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-160_CAGAGAGG-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1531303	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42779384615586	34.0	31.0	34.0	31.0	34.0
2	32.615584244267794	34.0	31.0	34.0	31.0	34.0
3	32.722391975983854	34.0	31.0	34.0	31.0	34.0
4	36.24636404421594	37.0	35.0	37.0	35.0	37.0
5	35.960549936883815	37.0	35.0	37.0	35.0	37.0
6	36.05193093724756	37.0	36.0	37.0	35.0	37.0
7	36.049692320853545	37.0	36.0	37.0	35.0	37.0
8	36.07598169663352	37.0	36.0	37.0	35.0	37.0
9	37.94296230073343	39.0	38.0	39.0	35.0	39.0
10-14	38.12535233066219	39.2	38.2	39.4	35.2	39.4
15-19	39.247802427083336	40.0	39.0	41.0	36.0	41.0
20-24	39.17744378480288	40.0	39.0	41.0	36.0	41.0
25-29	38.97723938371439	40.0	38.6	41.0	35.8	41.0
30-34	38.77135367722782	40.0	38.0	41.0	35.0	41.0
35-39	38.535432373606014	40.0	38.0	41.0	34.2	41.0
40-44	38.290646723737886	40.0	38.0	41.0	33.8	41.0
45-49	38.11052600301835	40.0	38.0	41.0	33.2	41.0
50-54	38.0629969379019	40.0	37.4	41.0	33.0	41.0
55-59	37.68406513929641	39.6	36.4	41.0	33.0	41.0
60-64	37.12793666570235	38.8	35.4	40.6	31.8	41.0
65-69	36.39070242793229	37.6	35.0	40.0	31.0	41.0
70-74	35.81500526022609	36.6	35.0	39.2	31.0	40.8
75-79	34.62094804228816	35.2	33.6	37.4	30.2	39.2
80-84	34.414355094974674	35.0	34.0	36.4	30.8	38.2
85-89	33.77636692411626	35.0	34.0	35.6	30.0	36.8
90-94	33.352616954319295	35.0	34.0	35.0	29.4	36.0
95-99	33.07528359834729	35.0	33.4	35.0	29.0	35.4
100-101	32.70391914598221	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	22.0
15	56.0
16	151.0
17	274.0
18	498.0
19	887.0
20	1210.0
21	1713.0
22	2507.0
23	3347.0
24	4606.0
25	6141.0
26	7870.0
27	10157.0
28	13103.0
29	16544.0
30	20935.0
31	26341.0
32	34559.0
33	46969.0
34	67063.0
35	107755.0
36	195464.0
37	376923.0
38	484513.0
39	101614.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.12288684865111	23.51905534045189	10.86473415124244	24.493323659654557
2	15.506041408137941	23.52589641434263	39.4704460845144	21.49761609300503
3	15.991609759792805	27.364016135278256	32.938157895596106	23.706216209332837
4	11.623565029259396	18.354434099587085	43.433664010323234	26.588336860830285
5	10.946559890498483	39.11720933087704	38.58942351709622	11.346807261528253
6	25.816510514248325	43.31983937862069	19.48445212998342	11.379197977147566
7	23.80175575963738	33.80741760448455	26.689427239416368	15.701399396461706
8	21.189797185795364	38.129096592901604	24.277625003020304	16.40348121828273
9	22.74487805483304	15.033993925434745	25.36800358910026	36.85312443063195
10-14	20.48954387211414	25.892328298187884	32.68160514280975	20.936522686888225
15-19	19.801640742640384	31.56926920459207	28.66912447620693	19.95996557656061
20-24	19.774616820906548	29.990862632293307	30.22363302575912	20.010887521041024
25-29	19.798311678709496	30.357896315408993	29.79619807664825	20.04759392923326
30-34	19.71293009708104	30.167313764739305	29.879956504560163	20.239799633619494
35-39	19.799497895441313	30.339512471996056	29.80206407987262	20.058925552690013
40-44	19.58876976166308	30.026097992132446	30.189007899961005	20.19612434624347
45-49	19.653735272079892	29.84498686858735	30.27983015538947	20.22144770394329
50-54	19.32063936933874	29.556523177674286	30.599270894613724	20.52356655837325
55-59	19.41401154708671	29.488202500366178	30.5283340295418	20.56945192300531
60-64	19.080805351166372	29.131060087719067	30.655546106349046	21.13258845476551
65-69	19.390665253954374	29.2491549139901	29.984334991035965	21.37584484101956
70-74	19.278010289919415	29.11215714363895	29.76177181696843	21.84806074947321
75-79	19.674199703046128	29.433972170762058	29.19897946922369	21.692848656968124
80-84	19.647165107707846	29.389764172894168	28.84021796385006	22.122852755547935
85-89	19.8898992170849	29.27746165293803	28.677952075282732	22.154687054694342
90-94	19.78140877447331	28.74329637030942	28.960098219766728	22.515196635450543
95-99	20.013915250456225	28.578899496208898	28.69596575286285	22.711219500472026
100-101	19.882191794240526	27.86092231939803	29.31187665402492	22.945009232336524
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.61621050830567
2	37.00365750114297
3	39.69782596912564
4	38.211901890089685
5	22.29336715202674
6	37.19570849139589
7	39.50315515609908
8	37.5932784040781
9	59.598002485464995
10-14	41.426066559002365
15-19	39.76160631920099
20-24	39.78550434194757
25-29	39.845905607942754
30-34	39.95272973070053
35-39	39.858423448131326
40-44	39.784894107906545
45-49	39.87518297602318
50-54	39.84420592771199
55-59	39.983463470092026
60-64	40.21339380593189
65-69	40.76651009497393
70-74	41.12607103939262
75-79	41.36704836001425
80-84	41.770017863255774
85-89	42.044586271779245
90-94	42.296605409923856
95-99	42.72513475092825
100-101	42.82720102657705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	6.0
9	10.0
10	16.0
11	25.0
12	52.0
13	93.0
14	139.0
15	187.5
16	289.0
17	452.0
18	654.5
19	917.5
20	1352.0
21	2053.5
22	3018.5
23	4348.5
24	6075.5
25	8377.5
26	11405.5
27	14939.0
28	19191.5
29	23999.0
30	29271.0
31	34713.0
32	40319.0
33	46577.0
34	53417.5
35	61241.0
36	69896.5
37	77469.5
38	82413.0
39	83745.0
40	82024.5
41	78410.5
42	73538.0
43	68908.5
44	64801.5
45	60525.0
46	56142.0
47	51286.5
48	45577.5
49	39412.5
50	34101.5
51	30018.5
52	26087.0
53	22624.5
54	19432.5
55	16670.0
56	14552.5
57	12349.5
58	10195.5
59	8430.0
60	6926.5
61	5835.0
62	4783.0
63	3534.0
64	2786.0
65	2347.5
66	1898.5
67	1589.0
68	1429.5
69	1443.0
70	1398.0
71	1176.5
72	1005.5
73	858.0
74	659.5
75	529.5
76	416.0
77	295.0
78	196.0
79	131.5
80	94.0
81	66.0
82	48.0
83	32.5
84	24.5
85	17.0
86	8.5
87	4.5
88	4.5
89	3.0
90	1.5
91	2.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013256684013549246
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.00135832033242278
20-24	5.61613214367111E-4
25-29	0.017462252735089008
30-34	0.007052817110656742
35-39	0.04086715692452767
40-44	0.04987908989925573
45-49	0.0505843716103214
50-54	0.050270913072069996
55-59	0.04121979778006051
60-64	0.05097619478313566
65-69	0.04116755469035194
70-74	0.0037092593693083603
75-79	0.0013975026497042061
80-84	2.089723588342738E-4
85-89	0.0033566185137755234
90-94	0.0028080660718355546
95-99	0.0021811489953327333
100-101	3.2651931067855284E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1531303.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.021090456026943
#Duplication Level	Relative count
1	100.0
2	6.80232903570792
3	2.4437050680292316
4	1.6606262254173847
5	1.2833462064048482
6	0.9910284593904105
7	0.7836729843740716
8	0.6333551185312816
9	0.5073970649396946
10++	3.722892282098509
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	411555	3.5765522	6.4010043	90-94
CCGAG	265705	3.5166936	8.728042	95-97
GAGCC	257585	3.4092226	8.090552	95-97
CTCCG	276530	3.403762	8.296116	95-97
CTGTC	387165	3.3645947	6.0343895	90-94
TCTCT	576440	3.3284254	5.058811	90-94
CGAGC	248445	3.2882516	8.145743	85-89
CTCTT	530430	3.062759	4.813798	95-97
TCTCC	370195	3.0275793	6.1096554	95-97
AGCCC	239305	2.9806764	7.531529	90-94
GCCCA	238815	2.9745731	7.6305804	90-94
TGTCT	476880	2.9259412	5.029208	90-94
ATCTC	472500	2.760786	6.7099123	95-97
GAGAG	274750	2.7606428	6.372698	95-97
ACACA	446200	2.6696405	6.779631	6
CCCAC	227260	2.663878	7.2003403	90-94
CCACG	210595	2.6230774	7.443349	90-94
TCCGA	289780	2.548304	5.93845	95-97
GAGGA	252890	2.5409968	6.5091157	95-97
TACAC	405765	2.3991184	8.416999	5
GAGAC	248585	2.3505836	6.2280226	95-97
CGAGA	244580	2.3127127	6.26776	95-97
AGAGG	224530	2.2560403	5.9342093	95-97
CAGAG	237065	2.241652	5.7406197	90-94
ACGAG	230865	2.1830258	6.010997	95-97
GACCA	240535	2.140461	5.323165	95-97
ACCAG	223035	1.9847327	5.0572014	90-94
CACGA	221500	1.9710732	5.3490543	95-97
CTTTG	317380	1.9473143	8.002634	9
GGATC	208295	1.9464061	5.6351013	95-97
AGACC	217090	1.9318297	5.5245275	95-97
CCAGA	216920	1.9303169	5.244474	90-94
TATAC	420775	1.7564945	5.4548125	5
TGGAG	167135	1.6595632	5.1370635	5
GCTTT	267030	1.6383871	5.004432	1
GTCTT	218670	1.3416698	6.027931	1
AAGAC	203095	1.291202	5.678174	5
GAGTC	137585	1.2856587	5.740575	9
CCATG	145035	1.2754271	5.155055	9
GTGTT	188975	1.2320621	5.643374	1
GATTG	184745	1.2188412	5.607645	7
TATGA	270735	1.200917	5.542519	4
GACTT	189425	1.1760885	5.780003	7
GTTCT	190320	1.1677259	5.2662582	1
TCATA	273275	1.1407665	5.3643255	2
GTGTA	161745	1.0671003	9.690496	1
TGGAC	113005	1.0559717	6.905001	5
GCCGT	80110	1.0477927	5.6634774	95-97
TGCCG	76575	1.0015569	5.817801	95-97
GTCCA	113675	0.99964964	6.6993303	1
AGACT	151795	0.9536879	5.1695743	6
GGACT	101440	0.9479029	6.139289	6
GTATA	165095	0.73232275	6.8337364	1
>>END_MODULE