##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-159_CAGAGAGG-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1732499	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68557499888889	33.0	31.0	34.0	30.0	34.0
2	31.882707003005486	33.0	31.0	34.0	30.0	34.0
3	32.025877071213316	34.0	31.0	34.0	30.0	34.0
4	35.55219829852716	37.0	35.0	37.0	33.0	37.0
5	35.42550096710013	37.0	35.0	37.0	33.0	37.0
6	35.56602918674123	37.0	35.0	37.0	33.0	37.0
7	35.5772857588951	37.0	35.0	37.0	33.0	37.0
8	35.629330810580555	37.0	35.0	37.0	33.0	37.0
9	37.43818553430622	39.0	37.0	39.0	35.0	39.0
10-14	37.563652042512004	39.2	37.2	39.4	34.0	39.4
15-19	38.61804572470172	40.0	38.0	41.0	34.0	41.0
20-24	38.51411654494461	40.0	38.0	41.0	34.0	41.0
25-29	38.33316821539292	40.0	38.0	41.0	33.8	41.0
30-34	38.07157106584189	40.0	38.0	41.0	33.0	41.0
35-39	37.76389273529163	40.0	37.8	41.0	32.4	41.0
40-44	37.72794777947924	40.0	37.8	41.0	32.6	41.0
45-49	37.57846509579515	40.0	37.0	41.0	32.0	41.0
50-54	36.87543888914222	39.2	36.4	40.2	31.2	40.6
55-59	37.50097725886133	40.0	36.6	41.0	32.0	41.0
60-64	37.04895610329356	39.0	35.4	41.0	31.2	41.0
65-69	36.37357043207528	38.0	35.0	40.2	30.8	41.0
70-74	35.52030460046441	36.6	34.6	39.2	29.8	41.0
75-79	34.5433389571942	35.4	34.0	37.6	29.0	39.4
80-84	33.61660272242582	35.0	33.8	36.4	28.4	38.0
85-89	32.848714371552305	35.0	33.0	35.4	26.4	36.6
90-94	32.25585423137329	35.0	32.6	35.0	25.4	36.0
95-99	31.72904307592674	35.0	32.0	35.0	24.2	35.0
100-101	30.791543891223025	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	32.0
7	154.0
8	316.0
9	479.0
10	632.0
11	751.0
12	1082.0
13	1519.0
14	1931.0
15	2356.0
16	2661.0
17	3197.0
18	3758.0
19	4270.0
20	5010.0
21	5633.0
22	6512.0
23	7432.0
24	8787.0
25	10155.0
26	12324.0
27	14571.0
28	17414.0
29	21245.0
30	26134.0
31	33199.0
32	43441.0
33	58855.0
34	84144.0
35	129346.0
36	227014.0
37	415342.0
38	491063.0
39	91695.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63990387335858	23.3753800778575	10.670934855838908	24.313781192945015
2	15.404388732833507	23.60157858118232	39.69352198552486	21.300510700459313
3	15.94704621995022	27.548425202987758	32.655350660882995	23.84917791617903
4	11.288584915682462	18.232830491956232	43.944829863789415	26.53375472857189
5	10.970380814796082	39.12021238880825	38.74288505575899	11.166521740636682
6	26.005662452055304	43.44913172849091	19.249046878291914	11.29615894116187
7	24.147870918288422	33.691335677558925	26.496002271882453	15.664791132270198
8	21.33268300003752	38.23648081180066	24.101486627281098	16.329349560880722
9	22.996674158756107	14.812230229945964	25.052381133787478	37.138714477510455
10-14	20.604402899246097	25.836421784845204	32.61318209830292	20.94599321760578
15-19	19.9642510738534	31.506881899219437	28.504861207334535	20.024005819592627
20-24	19.955809683743418	29.92459865639362	30.05077752764464	20.06881413221832
25-29	19.963780948506468	30.320300908812587	29.603850805306493	20.11206733737445
30-34	19.810099460606644	30.120461899139308	29.71942633408771	20.350012306166334
35-39	19.9759637863617	30.29534988160837	29.578835201854552	20.149851130175378
40-44	19.678063940854543	29.930103404565383	30.157092133524333	20.234740521055745
45-49	19.786258752335574	29.7429669597889	30.12129968200194	20.349474605873592
50-54	19.429127286009894	29.367091062655476	30.604525310215752	20.599256341118878
55-59	19.532243730690347	29.35661924716334	30.594249581915182	20.51688744023113
60-64	19.223525399409176	28.90341732551281	30.828988714516605	21.04406856056141
65-69	19.634959628475524	28.9488994512179	30.207307804168465	21.208833116138116
70-74	19.449936270696725	28.60143881934928	30.23410747973842	21.714517430215576
75-79	19.610943753946213	28.727671595483656	29.937838419142672	21.723546231427463
80-84	19.49007404232877	28.375557580344907	29.978934442544343	22.155433934781975
85-89	19.710351702101107	28.157341817294736	30.06369320860152	22.068613272002636
90-94	19.755093577463917	27.648698380459763	30.413362713606755	22.182845328469565
95-99	20.237540523316493	27.23934617764683	30.04621505296894	22.476898246067737
100-101	20.567273948916252	26.530594574375698	30.59590202093581	22.306229455772243
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.95368506630359
2	36.70489943329282
3	39.79622413612925
4	37.822339644254356
5	22.136902555432762
6	37.301821393217175
7	39.81266205055862
8	37.66203256091824
9	60.13538863626656
10-14	41.55039611685187
15-19	39.98825689344603
20-24	40.02462381596174
25-29	40.07584828588092
30-34	40.160111766772985
35-39	40.12581491653708
40-44	39.91280446191029
45-49	40.135733358209166
50-54	40.028383627128775
55-59	40.04913117092148
60-64	40.26759395997059
65-69	40.84379274461364
70-74	41.164453700912304
75-79	41.33448998537368
80-84	41.645507977110746
85-89	41.778964974103744
90-94	41.93793890593348
95-99	42.71443876938423
100-101	42.87350340468849
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	5.5
2	4.5
3	3.5
4	6.0
5	9.0
6	10.5
7	17.5
8	24.0
9	33.5
10	51.0
11	63.5
12	81.5
13	106.5
14	157.5
15	217.0
16	291.0
17	451.0
18	621.0
19	839.0
20	1186.5
21	1754.0
22	2637.0
23	3780.5
24	5352.5
25	7486.0
26	10216.0
27	13702.5
28	17906.5
29	22875.5
30	28786.5
31	35177.0
32	41852.0
33	49313.0
34	58213.5
35	68342.5
36	79613.5
37	90466.0
38	98118.5
39	101167.5
40	99939.0
41	95773.0
42	89701.0
43	83550.5
44	78026.5
45	72126.5
46	65850.0
47	59229.5
48	52245.5
49	44955.0
50	38298.0
51	33606.0
52	29520.5
53	25637.0
54	22112.0
55	18844.0
56	16285.0
57	13621.0
58	10987.5
59	9021.5
60	7331.0
61	5895.5
62	4669.0
63	3293.0
64	2259.5
65	1761.0
66	1333.5
67	940.0
68	688.0
69	687.5
70	718.5
71	630.5
72	524.0
73	400.0
74	288.5
75	212.5
76	146.0
77	107.0
78	80.5
79	52.5
80	35.0
81	25.5
82	19.5
83	22.5
84	15.0
85	10.5
86	13.0
87	7.0
88	6.0
89	5.5
90	6.5
91	9.5
92	6.0
93	3.5
94	4.0
95	6.0
96	5.5
97	4.5
98	3.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035613296169290716
2	0.03255413134437595
3	0.028744605335991536
4	0.027417043242160603
5	0.03353537289199013
6	0.001962483095228338
7	8.658013655419137E-4
8	0.0031168849159508892
9	2.8860045518063794E-4
10-14	0.007584419962147164
15-19	0.024184718144137456
20-24	0.020479088299618066
25-29	0.00234343569606678
30-34	0.001974027113435563
35-39	0.006522370287082417
40-44	0.014683991159590858
45-49	0.009316022693230992
50-54	0.005298704357116512
55-59	0.00435209486412402
60-64	0.011705634462126673
65-69	0.015191927960708779
70-74	0.018978365932678747
75-79	0.008242428999959018
80-84	0.011116889533558171
85-89	0.009443006893510473
90-94	0.01040116040471019
95-99	0.009408374838888796
100-101	0.006262629877419842
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1732499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.39003122916594
#Duplication Level	Relative count
1	100.0
2	7.353076287018038
3	2.710930082993906
4	1.9379536336630632
5	1.5594279239273574
6	1.1551771659571861
7	1.0075643891832298
8	0.7827764677058769
9	0.6804887759164548
10++	5.312834961565553
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	512655	3.8435378	6.714375	90-94
CTGTC	484355	3.6313636	6.206779	90-94
GCTGC	319605	3.6212046	9.011771	90-94
GACGC	295795	3.4716845	9.25223	95-97
TCTCT	691930	3.4326904	5.194116	90-94
CGCTG	291355	3.301125	8.686468	90-94
GCCGA	272030	3.1927595	9.274787	90-94
CTCTT	643505	3.1924522	4.9512925	80-84
CTGCC	288655	3.0977926	8.461886	90-94
CGACG	263820	3.0964005	9.343309	95-97
TGTCT	588560	3.0826864	5.117966	90-94
ACACA	554255	3.0564113	7.383334	6
TGCCG	262140	2.9701116	8.787576	90-94
CCGAC	260710	2.898284	8.576635	95-97
TACAC	511905	2.7250953	9.136309	5
AGAAG	442435	2.7194684	5.1463103	5
CTGAC	344595	2.6762388	6.517788	95-97
TGACG	325045	2.665174	6.614788	95-97
CACAT	494445	2.632148	5.1208563	95-97
ATCTG	474580	2.5748863	5.260366	95-97
GACGA	292585	2.485101	7.176203	95-97
ACGCT	301980	2.3452766	6.0844803	85-89
CAAAG	379230	2.2078576	5.2049227	4
CCTTG	293360	2.1994135	5.54052	95-97
CTCCT	300770	2.1358674	5.4656715	90-94
ACTCC	282575	2.0786598	5.391361	90-94
CTTTG	371345	1.9449848	8.153356	9
TATAC	522575	1.9434512	5.5383954	5
AGAGA	309235	1.900742	5.0375457	8
GGTGG	145195	1.8336798	5.9701123	95-97
GAGAG	201495	1.8068515	6.0488353	7
GCTTT	312145	1.6349144	5.1704836	1
GTGTA	275210	1.5764455	10.51463	1
TTGAG	258455	1.4804702	5.544572	9
CTCGG	122650	1.3896551	5.905652	95-97
AAGAC	237390	1.3820724	5.8686	5
GAGTC	165410	1.3562628	7.069791	9
CCATG	168155	1.3059474	5.551446	9
TATGA	320905	1.2599916	6.5138516	4
AGAAC	213775	1.2445871	5.111726	5
GTCTT	233615	1.2235997	5.947962	1
GACTT	222415	1.206737	6.3067126	7
CGGTG	100535	1.2026048	5.683797	95-97
TCATA	322980	1.2011594	6.205871	2
GATTG	209055	1.1974994	6.2083864	7
GTGTT	211990	1.1722499	5.7672853	1
GTTCT	217715	1.1403207	5.139994	1
ACATG	195955	1.1013234	5.2603364	8
TGGAC	131480	1.078057	7.346315	5
GTCCA	132385	1.0281458	7.263585	1
AGACT	176320	0.990969	5.399169	6
GAGTA	166255	0.98650527	5.5726037	1
GGACT	119575	0.9804433	6.45334	6
ACACT	182185	0.9698507	5.0313907	6
GTATG	164840	0.944229	5.579484	3
GTATA	189265	0.7431243	6.919659	1
>>END_MODULE