##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-159_CAGAGAGG-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1732499	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45541151827505	34.0	31.0	34.0	31.0	34.0
2	32.63815967570544	34.0	31.0	34.0	31.0	34.0
3	32.74769740126834	34.0	31.0	34.0	31.0	34.0
4	36.259693079187926	37.0	37.0	37.0	35.0	37.0
5	35.98637113210455	37.0	35.0	37.0	35.0	37.0
6	36.076207836194996	37.0	36.0	37.0	35.0	37.0
7	36.074800043174626	37.0	36.0	37.0	35.0	37.0
8	36.099819971036055	37.0	36.0	37.0	35.0	37.0
9	37.968148899364444	39.0	38.0	39.0	35.0	39.0
10-14	38.15402571660936	39.4	38.2	39.4	35.2	39.4
15-19	39.2880256785141	40.4	39.0	41.0	36.0	41.0
20-24	39.21781611417958	40.0	39.0	41.0	36.0	41.0
25-29	39.030766886445534	40.0	39.0	41.0	36.0	41.0
30-34	38.832714246876904	40.0	38.0	41.0	35.0	41.0
35-39	38.609397061701046	40.0	38.0	41.0	34.6	41.0
40-44	38.3752979944	40.0	38.0	41.0	34.0	41.0
45-49	38.207600581587634	40.0	38.0	41.0	33.4	41.0
50-54	38.16262474033174	40.0	37.6	41.0	33.6	41.0
55-59	37.77687975577475	39.8	36.6	41.0	33.0	41.0
60-64	37.21237657280032	39.0	35.6	40.8	32.0	41.0
65-69	36.464680325933806	37.6	35.0	40.0	31.0	41.0
70-74	35.86658024045036	36.6	35.0	39.2	31.0	40.8
75-79	34.65485578923855	35.2	33.6	37.4	30.2	39.2
80-84	34.426802786033356	35.0	34.0	36.4	31.0	38.2
85-89	33.79527803479252	35.0	34.0	35.6	30.0	36.6
90-94	33.38654671662148	35.0	34.0	35.0	29.6	36.0
95-99	33.120862291984004	35.0	33.6	35.0	29.0	35.2
100-101	32.76020563359633	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	7.0
14	20.0
15	73.0
16	128.0
17	301.0
18	521.0
19	894.0
20	1289.0
21	1906.0
22	2693.0
23	3582.0
24	5017.0
25	6570.0
26	8562.0
27	11073.0
28	14077.0
29	18383.0
30	23021.0
31	29041.0
32	37859.0
33	51153.0
34	73189.0
35	116605.0
36	218555.0
37	431589.0
38	561052.0
39	115247.0
40	89.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.24493001150362	23.457502717173284	10.958217003299858	24.339350268023242
2	15.588742102240092	23.43313350208827	39.54309167895768	21.435032716713955
3	16.031870725466508	27.19955393913647	32.86154854923437	23.907026786162646
4	11.487510238101148	18.189216848032814	43.737283542443606	26.585989371422436
5	10.796600748398701	39.26380332687061	38.87274971010084	11.06684621462985
6	25.91366575103362	43.65566733371852	19.146042797138698	11.284624118109159
7	24.01998500432035	33.77808587479704	26.525383275834503	15.676545845048107
8	21.270142147268196	38.29635688101407	24.06841216069966	16.365088811018072
9	22.930749166377584	14.63296659911492	25.006767680673985	37.42951655383351
10-14	20.563798305222686	25.79451993911685	32.63859892559823	21.00308283006224
15-19	19.871035621569654	31.531740886351024	28.512944230646685	20.084279261432638
20-24	19.90752252773297	29.91483686917557	30.109308769336657	20.068331833754808
25-29	19.9076221857315	30.365453967311222	29.565253435129662	20.16167041182761
30-34	19.77310903996481	30.13487849621387	29.71973719300798	20.372275270813347
35-39	19.94986323607334	30.297459808128597	29.596859395781085	20.15581756001698
40-44	19.631482851227926	29.934040163566717	30.17291761280555	20.2615593723998
45-49	19.754020265076637	29.717638669389252	30.141977043292815	20.386364022241295
50-54	19.38930823363308	29.313976330661106	30.599811595167083	20.696903840538734
55-59	19.418842653668396	29.29173368429842	30.579985127253913	20.709438534779277
60-64	18.99799777427807	28.872245062726204	30.716498813506565	21.413258349489166
65-69	19.361025257006037	29.0352436715432	29.903879845880688	21.69985122557008
70-74	19.16802650604496	28.874976550346766	29.66946523246182	22.28753171114645
75-79	19.664809473911085	29.296218107842936	28.900921539022235	22.13805087922375
80-84	19.711419857659717	29.25077700026124	28.471993185324155	22.565809956754883
85-89	20.017341996712386	29.173262847848626	28.265930780474125	22.543464374964863
90-94	19.844107729803664	28.61768008917761	28.6257033957866	22.912508785232127
95-99	20.14057932433172	28.38167193047388	28.318340006312415	23.159408738881993
100-101	20.03142861678011	27.513834796298408	29.049305987454527	23.405430599466953
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.58428027952685
2	37.023774818954045
3	39.93889751162916
4	38.07349960952359
5	21.863446963028547
6	37.19828986914278
7	39.69653084936846
8	37.63523095828627
9	60.36026572021109
10-14	41.56688113528493
15-19	39.95531488300229
20-24	39.97585436148778
25-29	40.06929259755911
30-34	40.14538431077816
35-39	40.10568079609032
40-44	39.89304222362773
45-49	40.14038428731793
50-54	40.08621207417181
55-59	40.12828118844767
60-64	40.41125612376724
65-69	41.06087648257611
70-74	41.45555821719141
75-79	41.80286035313483
80-84	42.2772298144146
85-89	42.560806371677245
90-94	42.756616515035795
95-99	43.29998806321371
100-101	43.436859216247065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	2.0
7	2.0
8	3.0
9	6.5
10	12.5
11	25.0
12	43.5
13	67.0
14	115.0
15	181.0
16	254.5
17	386.0
18	562.0
19	791.5
20	1150.0
21	1726.5
22	2597.0
23	3752.5
24	5323.5
25	7451.0
26	10252.0
27	13779.5
28	17939.0
29	22931.5
30	28579.5
31	34688.0
32	41105.5
33	48384.5
34	56850.5
35	66511.0
36	78132.0
37	88636.5
38	95608.0
39	98368.5
40	96927.5
41	92848.0
42	87026.0
43	81145.0
44	76513.0
45	71888.0
46	66800.0
47	61706.0
48	55511.5
49	47966.5
50	41441.0
51	36768.5
52	32117.5
53	27813.5
54	24097.0
55	20451.5
56	17400.0
57	14483.5
58	11613.5
59	9464.5
60	7553.5
61	6052.5
62	4778.0
63	3341.0
64	2253.5
65	1704.0
66	1319.5
67	915.0
68	663.0
69	697.5
70	712.0
71	549.5
72	468.5
73	416.0
74	308.5
75	229.5
76	149.0
77	86.5
78	44.5
79	19.5
80	15.0
81	8.5
82	5.5
83	5.0
84	2.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011774898571370027
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011890338753442282
20-24	7.041851106407565E-4
25-29	0.01778933205733452
30-34	0.007215011379515947
35-39	0.04362484480510523
40-44	0.0519365379143076
45-49	0.0524791067700472
50-54	0.05244447471542552
55-59	0.04275904343956331
60-64	0.05344880429945414
65-69	0.043278524258888465
70-74	0.003694085826312165
75-79	0.0015930745125971213
80-84	1.6161625490115723E-4
85-89	0.003301589207266498
90-94	0.0026666682058690944
95-99	0.002274171586823427
100-101	8.658013655419136E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1732499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.001556379406818
#Duplication Level	Relative count
1	100.0
2	7.512361529319762
3	2.816519806156442
4	1.946440556899981
5	1.4846150530483562
6	1.2099828200912568
7	1.050499079427829
8	0.8146601887942659
9	0.7463100142242255
10++	5.572078645804469
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	514480	3.8886364	6.779349	90-94
CCGAG	337645	3.8526318	9.338907	95-97
GAGCC	327860	3.740982	9.09732	95-97
CTCCG	352430	3.709722	8.839806	95-97
CTGTC	485750	3.671484	6.256733	90-94
CGAGC	314725	3.5911078	9.078878	95-97
TCTCT	697155	3.532445	5.351533	90-94
AGCCC	308450	3.2857828	8.360418	90-94
CTCTT	646245	3.274487	5.108304	90-94
GCCCA	306040	3.2601101	8.526738	90-94
TCTCC	457150	3.2258518	6.6262364	95-97
TGTCT	589330	3.1985102	5.3064036	90-94
ATCTC	585820	3.0039716	7.555115	95-97
GAGAG	330245	2.9330711	7.1461453	90-94
CCCAC	293215	2.916064	7.9855647	90-94
ACACA	554720	2.9132426	6.992133	6
CCACG	267390	2.848389	8.269511	90-94
TCCGA	371155	2.8390284	6.411388	95-97
GAGGA	305450	2.7128544	7.249079	95-97
AGAGA	445655	2.6852696	5.471023	90-94
AGAAG	444835	2.6803288	5.1563234	5
TACAC	513615	2.6653543	9.012579	5
GAGAC	308695	2.5596042	7.0692086	95-97
CGAGA	305450	2.5326974	7.0759087	95-97
AGAGG	272820	2.423051	6.9792128	95-97
ACGAG	290610	2.4096487	6.7850995	95-97
CAGAG	288325	2.390702	6.514781	90-94
GACCA	294515	2.2798562	6.1530957	95-97
CACGA	280720	2.1730683	6.1118083	95-97
ACCAG	277505	2.1481807	5.827657	90-94
CAAAG	379635	2.1355624	5.223013	4
AGACC	272055	2.105992	6.306986	95-97
GGATC	255790	2.095758	6.2927303	95-97
CCAGA	267410	2.070035	5.9004755	90-94
CTTTG	371710	2.0174067	8.31539	9
TATAC	525310	1.9574617	5.8247724	5
GCTTT	313165	1.6996614	5.3556395	1
TTGAG	257755	1.5164393	5.585177	9
GTCTT	254920	1.3835444	6.3057065	1
GAGTC	164970	1.3516446	6.5784597	9
AAGAC	237025	1.3333375	5.978508	5
GTATG	226070	1.3300283	5.7962613	3
GACTC	172275	1.3177612	5.2774687	7
CCATG	169645	1.297644	5.544495	9
TATGA	319730	1.2761571	6.419076	4
GATTG	211290	1.2430737	6.4295144	7
GTGTT	212370	1.234598	5.7760644	1
GACTT	223225	1.2260766	6.295476	7
TCATA	323690	1.2061654	6.188649	2
GTTCT	217335	1.1795568	5.181943	1
ACTCA	220495	1.1442369	5.0019565	8
GTGTA	192590	1.1330568	10.775133	1
ACATG	195960	1.0892508	5.5194616	8
TGGAC	132040	1.0818402	7.22995	5
GCCGT	92870	1.0470973	6.365248	95-97
CCGTC	97840	1.029876	5.1208267	95-97
GTCCA	131645	1.0069753	6.976875	1
GGACT	121090	0.9921237	6.48312	6
GAGTA	166510	0.99138844	5.410418	1
TGCCG	87880	0.9908357	6.7861967	95-97
AGACT	175440	0.9751895	5.6380434	6
ACACT	183320	0.95132107	5.0422144	6
GGATA	148870	0.8863612	5.08995	1
GTATA	189115	0.7548258	7.0333686	1
>>END_MODULE