##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-158_CAGAGAGG-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1269630	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.557130817639784	31.0	31.0	34.0	30.0	34.0
2	31.756781109457087	33.0	31.0	34.0	30.0	34.0
3	31.899894457440357	34.0	31.0	34.0	30.0	34.0
4	35.42942038231611	37.0	35.0	37.0	33.0	37.0
5	35.28837771634256	37.0	35.0	37.0	32.0	37.0
6	35.4334412387861	37.0	35.0	37.0	33.0	37.0
7	35.445666847821805	37.0	35.0	37.0	33.0	37.0
8	35.500105542559645	37.0	35.0	37.0	33.0	37.0
9	37.29145735371723	39.0	37.0	39.0	34.0	39.0
10-14	37.40043808038563	39.2	37.2	39.4	33.2	39.4
15-19	38.42240274725707	40.0	38.0	41.0	33.8	41.0
20-24	38.30551089687547	40.0	38.0	41.0	33.6	41.0
25-29	38.10948449548294	40.0	38.0	41.0	33.0	41.0
30-34	37.82615517906792	40.0	38.0	41.0	32.4	41.0
35-39	37.491004465868016	40.0	37.0	41.0	31.4	41.0
40-44	37.433953671542106	40.0	37.0	41.0	31.2	41.0
45-49	37.250504635208685	40.0	36.6	41.0	31.0	41.0
50-54	36.54358340618921	38.6	35.4	40.2	30.2	40.6
55-59	37.15329599962193	39.6	35.8	41.0	31.0	41.0
60-64	36.68037191937809	39.0	35.0	41.0	30.6	41.0
65-69	35.99921520442964	37.6	35.0	40.0	29.6	41.0
70-74	35.175549727085844	36.4	34.2	39.2	29.0	40.8
75-79	34.241251388199714	35.2	34.0	37.6	28.4	39.4
80-84	33.36419334766821	35.0	33.2	36.2	27.0	38.0
85-89	32.623420524089696	35.0	33.0	35.2	25.8	36.6
90-94	32.05738994825264	35.0	32.2	35.0	25.0	36.0
95-99	31.552247505178673	34.6	31.6	35.0	23.6	35.0
100-101	30.63576711325347	34.0	30.0	35.0	19.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	33.0
7	139.0
8	259.0
9	402.0
10	438.0
11	736.0
12	1131.0
13	1540.0
14	2023.0
15	2273.0
16	2610.0
17	2893.0
18	3309.0
19	3858.0
20	4330.0
21	4977.0
22	5534.0
23	6461.0
24	7522.0
25	8654.0
26	10137.0
27	11665.0
28	14142.0
29	17061.0
30	20860.0
31	26388.0
32	33586.0
33	46084.0
34	64669.0
35	99974.0
36	169976.0
37	295606.0
38	336006.0
39	64318.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21946282655439	23.08353536364274	10.283336182899609	24.413665626903267
2	15.71012569865414	23.434991089057426	39.16363200879906	21.69125120348937
3	16.269879411760073	27.225950500993477	32.51895959750346	23.98521048974299
4	11.6042918515799	18.032536325454647	43.340169181329074	27.023002641636378
5	11.389044059655959	38.6977421405897	38.1335333526104	11.779680447143933
6	26.45670258834575	42.76551770853189	19.10265639792882	11.675123305193546
7	24.21470659354248	33.58994534589456	26.151898873758388	16.043449186804565
8	21.668429704416152	37.50583850364173	24.118750241219796	16.70698155072232
9	23.072360850425678	14.677979211151657	25.005926956269697	37.24373298215297
10-14	20.918464843606614	25.597954780006994	32.25936598243232	21.224214393954075
15-19	20.2455346837909	31.150282850497717	28.296126500971617	20.30805596473976
20-24	20.20642707034459	29.654921297867503	29.813031425707464	20.32562020608044
25-29	20.189885681069562	30.032923599919975	29.381304121121037	20.395886597889422
30-34	20.120796359316635	29.83047205601604	29.475685325814794	20.573046258852536
35-39	20.244482939189297	29.98878030122103	29.368341356105866	20.39839540348381
40-44	19.98549298486248	29.742516236103878	29.804101729340815	20.46788904969283
45-49	20.117720027544255	29.549296009848124	29.81369502787115	20.519288934736466
50-54	19.782535511422935	29.25693226738283	30.203073873243174	20.757458347951058
55-59	19.902966992614772	29.25268862685125	30.202366341348686	20.64197803918529
60-64	19.641217851576524	28.865219277025677	30.413488958519398	21.0800739128784
65-69	19.95985030990601	28.93460739750635	29.909770330349524	21.195771962238116
70-74	19.861626575461774	28.621663556549613	29.960699716465722	21.556010151522887
75-79	20.149087493670915	28.8935236516259	29.627996487536713	21.329392367166474
80-84	20.27436231384262	28.65377795639525	29.545606716576984	21.52625301318515
85-89	20.501042275816175	28.5275112923716	29.53588478756226	21.435561644249965
90-94	20.619361774495086	28.10640694142429	29.827111878431495	21.447119405649133
95-99	21.094000338392522	27.815802300628047	29.424647641697142	21.665549719282293
100-101	21.375302758797236	27.186026819998823	29.92635330720911	21.512317113994843
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.63312845345766
2	37.40137690214351
3	40.25508990150306
4	38.62729449321628
5	23.168724506799894
6	38.13182589353929
7	40.258155780347046
8	38.375411255138474
9	60.316093832578645
10-14	42.142679237560685
15-19	40.553590648530665
20-24	40.53204727642503
25-29	40.585772278958984
30-34	40.69384261816917
35-39	40.64287834267311
40-44	40.453382034555304
45-49	40.637008962280724
50-54	40.53999385937399
55-59	40.54494503180007
60-64	40.721291764454925
65-69	41.15562227214413
70-74	41.41763672698466
75-79	41.47847986083739
80-84	41.80061532702777
85-89	41.93660392006614
90-94	42.066481180144216
95-99	42.759550057674815
100-101	42.88761987279208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	3.5
3	5.0
4	7.0
5	8.0
6	11.5
7	19.5
8	25.0
9	32.5
10	39.0
11	44.0
12	60.0
13	82.0
14	131.0
15	204.5
16	277.5
17	408.5
18	590.0
19	757.5
20	1113.5
21	1651.5
22	2372.0
23	3400.0
24	4688.0
25	6497.5
26	8697.0
27	11282.5
28	14475.5
29	18115.5
30	22380.5
31	26905.5
32	31455.5
33	36462.0
34	42154.5
35	48543.5
36	56054.5
37	63319.0
38	68284.0
39	70217.5
40	68885.5
41	66020.5
42	62179.5
43	57922.5
44	54034.5
45	49640.0
46	45462.5
47	41122.0
48	36268.0
49	31525.5
50	27269.5
51	24379.5
52	21932.5
53	19517.0
54	17346.0
55	15297.0
56	13631.0
57	11988.0
58	10298.5
59	8758.5
60	7498.0
61	6476.5
62	5435.0
63	4312.5
64	3411.0
65	2917.5
66	2476.0
67	2041.5
68	1698.5
69	1603.5
70	1527.0
71	1241.5
72	1048.5
73	872.5
74	665.5
75	516.5
76	408.5
77	310.0
78	210.5
79	158.0
80	119.0
81	91.0
82	68.0
83	53.5
84	49.5
85	32.5
86	23.0
87	17.0
88	9.0
89	9.5
90	12.5
91	12.5
92	11.5
93	8.0
94	7.0
95	7.5
96	3.5
97	3.0
98	3.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033946897915140634
2	0.031820294101431126
3	0.02772461268243504
4	0.026858218536108945
5	0.033001740664603076
6	0.0012602096673834109
7	7.088679379031686E-4
8	0.0032292872726699903
9	5.513417294802423E-4
10-14	0.007419484416719832
15-19	0.023802210092704175
20-24	0.02060442806171877
25-29	0.0018903145010751164
30-34	0.0017485409134944826
35-39	0.006080511645124957
40-44	0.01379929585784835
45-49	0.008616683600734072
50-54	0.004142939281522964
55-59	0.0032607925143545756
60-64	0.010664524310232114
65-69	0.014224616620590252
70-74	0.01767444058505234
75-79	0.007372226554192954
80-84	0.009939903751486654
85-89	0.007907815662830903
90-94	0.008175610217149878
95-99	0.007750289454407976
100-101	0.004331970731630475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1269630.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.46214236788028
#Duplication Level	Relative count
1	100.0
2	6.617467055964557
3	2.7262793988571836
4	1.822296260471337
5	1.3864259229405127
6	1.0562392151945594
7	0.8639786005576752
8	0.7027662839366854
9	0.5594664469402499
10++	3.6816116455000865
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	327165	3.366853	5.9812093	90-94
GCTGC	217430	3.268638	7.538723	90-94
TCTCT	456800	3.218054	4.8402014	80-84
CTGTC	306555	3.1547554	5.434252	85-89
GACGC	185870	2.866546	7.5125184	95-97
CGCTG	189205	2.8443298	7.2091775	90-94
CTGCC	190630	2.7835333	7.258702	90-94
ACACA	359165	2.7319198	6.403599	6
GCCGA	176825	2.7270508	7.3793917	90-94
CGACG	171910	2.6512504	7.612253	95-97
TGCCG	169310	2.545247	7.206261	90-94
AGAAG	310610	2.5042267	5.021754	5
CCGAC	166380	2.4923472	7.2388597	95-97
TACAC	322785	2.393233	7.955894	5
CTGAC	218665	2.3085475	5.56605	95-97
TGACG	206820	2.2479892	5.737486	95-97
GACGA	191015	2.1299598	5.8229523	95-97
TGCAG	176630	1.919845	5.0838037	90-94
CTTTG	262465	1.9036223	7.4568305	9
TATAC	334660	1.7487495	5.4981365	5
GAGAG	150940	1.7328079	5.5953374	7
GCTTT	222260	1.6120212	5.0278316	1
GTGTA	181815	1.392783	9.409283	1
CCATG	125725	1.3273369	5.6473417	9
AAGAC	167565	1.3121979	5.360129	5
GAGTC	117775	1.2801322	6.478323	9
GTCTT	170170	1.2342196	6.2557626	1
TATGA	227710	1.2250338	5.517002	4
TCATA	225955	1.1807169	5.4955583	2
GACTT	157890	1.1748064	5.723069	7
GATTG	152900	1.1712815	5.45002	7
GTGTT	153480	1.1460494	5.6762247	1
GTTCT	156645	1.1361245	5.2635655	1
TGGAC	94540	1.027584	6.3008885	5
GTCCA	97190	1.0260798	7.451806	1
AGACT	123650	0.9438608	5.1936417	6
GGACT	85645	0.9309014	5.808764	6
GAGTA	116995	0.9194398	5.169574	1
GTATA	136785	0.73587567	6.8011036	1
>>END_MODULE