##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-158_CAGAGAGG-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1269630	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42979371943007	34.0	31.0	34.0	31.0	34.0
2	32.61748540913494	34.0	31.0	34.0	31.0	34.0
3	32.729579483786615	34.0	31.0	34.0	31.0	34.0
4	36.244559438576594	37.0	37.0	37.0	35.0	37.0
5	35.96053338374172	37.0	35.0	37.0	35.0	37.0
6	36.05333758654096	37.0	36.0	37.0	35.0	37.0
7	36.052544442081555	37.0	36.0	37.0	35.0	37.0
8	36.079773634838496	37.0	36.0	37.0	35.0	37.0
9	37.94808960090735	39.0	38.0	39.0	35.0	39.0
10-14	38.129694005340134	39.2	38.2	39.4	35.2	39.4
15-19	39.25425879980782	40.0	39.0	41.0	36.0	41.0
20-24	39.17454990824099	40.0	39.0	41.0	36.0	41.0
25-29	38.97527815190252	40.0	38.6	41.0	35.6	41.0
30-34	38.76256641698763	40.0	38.0	41.0	35.0	41.0
35-39	38.51624678055812	40.0	38.0	41.0	34.0	41.0
40-44	38.25809251514221	40.0	38.0	41.0	33.6	41.0
45-49	38.05788458054709	40.0	37.8	41.0	33.0	41.0
50-54	37.9893921851248	40.0	37.0	41.0	33.0	41.0
55-59	37.58213889085797	39.4	36.2	41.0	32.8	41.0
60-64	37.00253995258461	38.8	35.2	40.6	31.8	41.0
65-69	36.25229224262186	37.4	35.0	40.0	31.0	41.0
70-74	35.677494860707455	36.4	34.8	39.0	31.0	40.8
75-79	34.50205997022754	35.2	33.4	37.4	29.6	39.2
80-84	34.32757874341344	35.0	34.0	36.4	30.6	38.2
85-89	33.714280853477	35.0	34.0	35.4	30.0	36.6
90-94	33.30791742476155	35.0	34.0	35.0	29.0	36.0
95-99	33.032179768909046	35.0	33.2	35.0	29.0	35.2
100-101	32.66164000535589	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	8.0
14	21.0
15	50.0
16	120.0
17	270.0
18	481.0
19	733.0
20	1119.0
21	1540.0
22	2109.0
23	3053.0
24	3987.0
25	5296.0
26	6832.0
27	8508.0
28	11134.0
29	14116.0
30	17350.0
31	22323.0
32	29271.0
33	39449.0
34	56953.0
35	91633.0
36	167548.0
37	314508.0
38	391365.0
39	79790.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75673227633248	23.081606452273498	10.594031331962855	24.567629939431175
2	15.954475202881222	23.325786829381265	38.92097597249858	21.798761995238927
3	16.42360372707009	26.975654324488236	32.74938367870954	23.851358269732124
4	11.807455715444657	17.987445161188692	43.11382056189599	27.091278561470666
5	11.237683419578932	38.84761702228208	38.21956002929988	11.69513952883911
6	26.473382008931733	43.090349156840965	18.838638028401974	11.59763080582532
7	24.11899529784268	33.584823925080535	26.24103085150792	16.05514992556887
8	21.641974433496372	37.57842836101856	24.015500578908817	16.764096626576247
9	22.973307183982737	14.571410568433324	24.98255397241716	37.47272827516678
10-14	20.81971913077038	25.596795916920676	32.28334239109032	21.300142561218625
15-19	20.15263212117565	31.179094635632794	28.32811967850406	20.340153564687494
20-24	20.200169814322688	29.673666650388856	29.764465329050694	20.36169820623776
25-29	20.20719543611671	30.067380898625206	29.329668219146182	20.395755446111902
30-34	20.06068947873119	29.83501327956497	29.489251500104842	20.615045741598994
35-39	20.209321211076322	29.94438703684822	29.403276009961765	20.443015742113698
40-44	19.981667240878775	29.675192429352208	29.821372227193827	20.52176810257519
45-49	20.07497377809054	29.48943913089047	29.876885081447497	20.55870200957149
50-54	19.77400052514767	29.277581521610646	30.180766167124933	20.767651786116748
55-59	19.782495503917406	29.17582535343023	30.184205433625337	20.857473709027026
60-64	19.47022562543618	28.84232760669595	30.300281235874376	21.387165531993496
65-69	19.74074622725955	28.987852847363175	29.70232542013893	21.56907550523834
70-74	19.597281773268335	28.816146032380157	29.494915060928218	22.091657133423297
75-79	19.9340525736336	29.183122411523343	28.90146275563016	21.981362259212894
80-84	19.97562751047905	29.144831534145194	28.548956300750095	22.330584654625664
85-89	20.210571419344625	29.03385573171875	28.431599733142825	22.3239731157938
90-94	20.055409595277123	28.61122514531353	28.67648981615936	22.656875443249984
95-99	20.292758402484676	28.402845416840144	28.402057771281275	22.902338409393902
100-101	20.16016477241401	27.69759693769051	29.062128336602	23.08010995329348
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.32436221576364
2	37.753237198120146
3	40.27496199680222
4	38.89873427691532
5	22.932822948418043
6	38.071012814757054
7	40.17414522341154
8	38.40607106007262
9	60.44603545914951
10-14	42.119861691989
15-19	40.49278568586315
20-24	40.561868020560446
25-29	40.60295088222861
30-34	40.67573522033019
35-39	40.65233695319002
40-44	40.503435343453965
45-49	40.633675787662035
50-54	40.54165231126442
55-59	40.63996921294443
60-64	40.85739115742968
65-69	41.30982173249789
70-74	41.68893890669163
75-79	41.915414832846494
80-84	42.30621216510471
85-89	42.53454453513842
90-94	42.71228503852711
95-99	43.19509681187858
100-101	43.240274725707486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.5
5	0.5
6	1.0
7	2.0
8	4.5
9	7.0
10	13.5
11	28.5
12	38.0
13	53.5
14	102.0
15	154.5
16	231.5
17	352.5
18	529.0
19	768.5
20	1111.5
21	1624.0
22	2354.0
23	3373.0
24	4704.0
25	6437.0
26	8597.0
27	11303.0
28	14523.0
29	18088.5
30	22167.5
31	26589.0
32	31110.5
33	35958.5
34	41459.5
35	47704.5
36	55127.5
37	62017.5
38	66752.5
39	68636.0
40	67326.5
41	64454.0
42	60510.5
43	56378.0
44	53090.5
45	49636.5
46	46287.5
47	42458.5
48	37891.0
49	33287.0
50	28945.0
51	25820.5
52	23181.5
53	20750.5
54	18428.5
55	16290.0
56	14588.5
57	12689.0
58	10870.5
59	9319.5
60	7804.0
61	6629.5
62	5562.0
63	4345.5
64	3392.5
65	2917.0
66	2490.5
67	2027.0
68	1754.5
69	1633.0
70	1447.5
71	1206.5
72	978.0
73	811.0
74	691.5
75	563.0
76	401.5
77	271.0
78	181.0
79	125.5
80	98.5
81	62.5
82	34.0
83	21.0
84	18.0
85	10.5
86	7.5
87	5.5
88	4.0
89	2.5
90	1.5
91	1.0
92	0.0
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013232201507525814
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011971991840142404
20-24	6.301048336917054E-4
25-29	0.01731213030567961
30-34	0.006726369099658956
35-39	0.042327292203240316
40-44	0.05078644959555146
45-49	0.05143230705008546
50-54	0.05147956491261234
55-59	0.04136638233186046
60-64	0.051952143537881114
65-69	0.04142939281522963
70-74	0.0029614927183510154
75-79	9.92415113064436E-4
80-84	1.4177358758063374E-4
85-89	0.002914234855824138
90-94	0.002394398368028481
95-99	0.0018430566385482384
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1269630.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.3671390031169
#Duplication Level	Relative count
1	100.0
2	6.8672881243877075
3	2.766508597633172
4	1.9250764801692481
5	1.465894953210364
6	1.1341302897531598
7	0.8528515533437911
8	0.7092070704338784
9	0.5691687251702398
10++	3.9144624150303815
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	326205	3.343627	5.8529315	90-94
CCGAG	216415	3.2731214	7.832232	95-97
TCTCT	457045	3.2081847	4.8745365	80-84
GAGCC	211465	3.1982563	7.4363723	90-94
CTCCG	223415	3.1605952	7.378597	95-97
CTGTC	307840	3.155384	5.4234996	85-89
CGAGC	202185	3.0579028	7.3848853	95-97
TCTCC	297365	2.8808432	5.746641	95-97
AGCCC	198355	2.83544	6.9065223	90-94
GCCCA	198110	2.8319378	7.129716	90-94
ATCTC	377520	2.677695	6.3946676	95-97
GAGAG	224065	2.62505	5.934455	95-97
ACACA	361255	2.6162333	6.102784	6
AGAAG	313150	2.5386918	5.2121468	5
CCCAC	187385	2.5317142	6.6259966	90-94
TCCGA	236005	2.4443824	5.6532383	95-97
CCACG	170135	2.4320416	6.819186	90-94
GAGGA	206940	2.4244213	6.1029835	95-97
TACAC	323625	2.3194447	7.6867485	5
GAGAC	202520	2.2425082	5.8058424	95-97
CGAGA	200020	2.2148256	5.734254	95-97
AGAGG	183865	2.154084	5.690184	95-97
CAGAG	193680	2.1446228	5.4659085	90-94
ACGAG	187375	2.0748074	5.5696	95-97
CAAAG	268890	2.0603204	5.0117297	4
GACCA	194085	2.0312374	5.0679083	95-97
CTTTG	264265	1.9626254	7.6771593	9
GGATC	172615	1.8915765	5.302924	95-97
CACGA	178130	1.8642571	5.1626363	95-97
AGACC	174290	1.8240685	5.265821	95-97
TATAC	336300	1.7463827	5.5694623	5
GCTTT	224120	1.6644795	5.0636106	1
GTCTT	180600	1.3412681	5.8055053	1
CCATG	126765	1.3129473	5.5943384	9
AAGAC	169050	1.2953147	5.516989	5
GAGTC	118050	1.2936338	6.2165127	9
TATGA	226300	1.2433532	5.6342473	4
GATTG	152775	1.213021	5.643722	7
GTGTT	153035	1.2025027	5.9633017	1
TCATA	227005	1.1788212	5.3939776	2
GACTT	156155	1.1718566	5.6980453	7
GTTCT	156425	1.1617267	5.6146293	1
GTGTA	134405	1.0671648	9.383152	1
TGGAC	95865	1.0505227	6.8541036	5
GCCGT	67380	1.0085211	5.12629	95-97
GTCCA	96730	1.0018649	6.4338994	1
TGCCG	65870	0.98591995	5.3996606	95-97
AGACT	124480	0.94392806	5.1583714	6
GGACT	85570	0.9377065	6.1633797	6
GAGTA	116665	0.93600327	5.174467	1
GTATA	136810	0.75167114	6.9458947	1
>>END_MODULE