##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-157_CAGAGAGG-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1066943	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22988013417774	31.0	31.0	34.0	28.0	34.0
2	31.42267581304718	31.0	31.0	34.0	28.0	34.0
3	31.555937852350127	33.0	31.0	34.0	28.0	34.0
4	35.08588743728578	37.0	35.0	37.0	32.0	37.0
5	34.95397504833904	37.0	35.0	37.0	32.0	37.0
6	35.11071538029679	37.0	35.0	37.0	32.0	37.0
7	35.11020832415603	37.0	35.0	37.0	32.0	37.0
8	35.160709616165065	37.0	35.0	37.0	32.0	37.0
9	36.89057990914229	39.0	37.0	39.0	33.0	39.0
10-14	36.97335733961421	39.2	37.2	39.4	32.2	39.4
15-19	37.94052484528227	40.0	38.0	41.0	32.2	41.0
20-24	37.81468963196722	40.0	38.0	41.0	32.0	41.0
25-29	37.603698229427444	40.0	37.4	41.0	31.6	41.0
30-34	37.30891715864858	40.0	37.0	41.0	30.8	41.0
35-39	36.965189143187594	39.8	36.4	41.0	30.0	41.0
40-44	36.89867687402233	39.6	36.0	41.0	30.0	41.0
45-49	36.702301622485926	39.2	35.8	41.0	29.6	41.0
50-54	36.06126250418251	38.4	35.0	39.8	28.8	40.6
55-59	36.71497333971918	39.0	35.4	41.0	29.6	41.0
60-64	36.2421507053329	38.8	35.0	40.6	28.8	41.0
65-69	35.55808510857656	37.4	34.2	40.0	28.0	41.0
70-74	34.71979215384515	36.2	34.0	39.0	27.2	40.8
75-79	33.77472629746857	35.2	33.2	37.6	26.0	39.4
80-84	32.877335715216276	35.0	32.6	36.2	25.6	38.0
85-89	32.136553499109134	35.0	32.0	35.2	24.6	36.6
90-94	31.581594893073014	34.4	31.2	35.0	23.4	36.0
95-99	31.110561670117335	34.0	31.0	35.0	20.8	35.0
100-101	30.249799192646655	34.0	29.5	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	33.0
7	131.0
8	235.0
9	324.0
10	482.0
11	715.0
12	1323.0
13	1929.0
14	2448.0
15	2963.0
16	3287.0
17	3605.0
18	4072.0
19	4381.0
20	4953.0
21	5520.0
22	6062.0
23	6986.0
24	7861.0
25	8928.0
26	10390.0
27	12024.0
28	14143.0
29	16736.0
30	20169.0
31	24926.0
32	31635.0
33	41864.0
34	58599.0
35	86653.0
36	141984.0
37	240112.0
38	255649.0
39	45779.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.228218525890625	23.127032776633417	10.22761472853027	24.417133968945688
2	15.153941423598383	23.54981731790622	40.108212303125704	21.188028955369692
3	15.75314211569436	27.89568719659341	32.64987746545688	23.701293222255348
4	10.989411749264523	18.29777978613496	44.447965319588164	26.264843145012346
5	10.553416345316348	39.46885081750958	38.98534867564291	10.992384161531156
6	25.656740423043185	43.98914274145778	19.272477449444015	11.081639386055022
7	23.887847489080922	34.08167282133953	26.69465949350479	15.335820196074756
8	21.17194295312078	38.83872334363097	24.046031912660087	15.943301790588166
9	23.076930286614054	14.709074549646653	24.70851219375035	37.50548296998894
10-14	20.46304730371789	25.79305530274158	32.99620707179681	20.74769032174372
15-19	19.80025322707021	31.705431632534637	28.529274950487977	19.965040189907178
20-24	19.75531309555838	30.089796025501737	30.20509940932795	19.94979146961193
25-29	19.79063566616879	30.567796343284925	29.635453220320684	20.006114770225604
30-34	19.606656660343823	30.380755767929042	29.747253181900064	20.265334389827068
35-39	19.841674814939232	30.613010041871124	29.57011238617413	19.975202757015513
40-44	19.506004391372034	30.405489514999356	30.075836655358284	20.01266943827033
45-49	19.703124481544823	30.226679616681075	30.039046880439386	20.03114902133472
50-54	19.4897076974066	29.873828187681926	30.39734766627325	20.239116448638217
55-59	19.669249212208012	29.91789342266329	30.43595218322889	19.976905181899813
60-64	19.36706587410081	29.537125841451335	30.699522855756662	20.396285428691193
65-69	19.724266267021044	29.698771610887693	30.19340430992608	20.383557812165183
70-74	19.551716263699756	29.440513369882265	30.285155911654126	20.722614454763853
75-79	19.728046356033005	29.723781556005257	29.85669334588091	20.69147874208083
80-84	19.64848329871983	29.58063358643246	29.811692302154686	20.959190812693027
85-89	19.73593683390405	29.502607112447215	29.748002251111398	21.013453802537338
90-94	19.69522877526286	29.09229467029985	30.216613096895202	20.99586345754209
95-99	20.029747744079415	28.987665596962685	29.736027202650977	21.246559456306922
100-101	20.115167699429833	28.303537702026187	30.466950797606852	21.114343800937128
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.64535249483632
2	36.34197037896808
3	39.454435337949704
4	37.25425489427687
5	21.545800506847502
6	36.738379809098205
7	39.223667685155675
8	37.11524474370895
9	60.582413256603
10-14	41.210737625461604
15-19	39.765293416977386
20-24	39.705104565170316
25-29	39.79675043639439
30-34	39.87199105017089
35-39	39.81687757195475
40-44	39.51867382964236
45-49	39.73427350287954
50-54	39.72882414604482
55-59	39.646154394107825
60-64	39.763351302792
65-69	40.10782407918623
70-74	40.27433071846361
75-79	40.41952509811384
80-84	40.607674111412855
85-89	40.74939063644138
90-94	40.69109223280495
95-99	41.276307200386334
100-101	41.229511500366954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	2.0
2	2.0
3	2.5
4	7.5
5	12.0
6	11.5
7	16.5
8	23.5
9	33.5
10	39.0
11	44.0
12	70.0
13	112.5
14	158.5
15	220.5
16	325.0
17	453.5
18	643.0
19	979.0
20	1385.5
21	1980.5
22	2836.5
23	3891.5
24	5217.0
25	6882.0
26	9019.0
27	11449.5
28	14170.5
29	17202.0
30	20704.0
31	24707.5
32	28714.5
33	32880.0
34	37923.0
35	44039.5
36	50823.5
37	57074.0
38	61605.0
39	62655.5
40	60301.5
41	56503.0
42	51680.5
43	47006.0
44	43398.0
45	39686.5
46	35994.0
47	32320.5
48	28323.5
49	24374.0
50	20994.0
51	18518.5
52	16370.0
53	14355.5
54	12586.0
55	11086.0
56	9810.5
57	8403.5
58	6903.0
59	5927.0
60	4864.5
61	3960.5
62	3299.5
63	2374.5
64	1698.0
65	1365.0
66	1086.0
67	832.0
68	686.5
69	683.5
70	693.5
71	580.5
72	461.0
73	375.5
74	284.5
75	207.5
76	150.5
77	121.0
78	82.0
79	53.5
80	46.5
81	33.5
82	22.0
83	18.0
84	15.0
85	11.0
86	10.0
87	12.0
88	10.0
89	7.5
90	5.0
91	3.0
92	3.0
93	2.5
94	5.0
95	5.5
96	3.0
97	4.5
98	4.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033741258905114896
2	0.0314918416447739
3	0.028586344350166788
4	0.02708673284327279
5	0.03280400171330614
6	6.560800342661229E-4
7	2.811771575426241E-4
8	0.002905497294607116
9	2.811771575426241E-4
10-14	0.0058859751645589315
15-19	0.02178185713763528
20-24	0.017995338082727942
25-29	0.0017058080890919195
30-34	0.001649572657583395
35-39	0.0047050311028799105
40-44	0.011603244034592288
45-49	0.0067107614933506286
50-54	0.004555069952190511
55-59	0.0034678516096923644
60-64	0.008978923897527797
65-69	0.012296814356530761
70-74	0.016139568842946622
75-79	0.0065608003426612296
80-84	0.010740967418128241
85-89	0.008641511308476648
90-94	0.010309829109896218
95-99	0.009916181089336545
100-101	0.005998446027575981
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1066943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.12682886119761
#Duplication Level	Relative count
1	100.0
2	6.411948634014419
3	2.58883159395294
4	1.7511131447880186
5	1.2372186339557558
6	0.8987287563870165
7	0.6828461308319107
8	0.5596522412488341
9	0.4505376533323947
10++	2.746637100133167
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
ACACA	248840	2.2022061	7.943925	6
CTTTG	244210	2.1472533	9.282809	9
GACTC	157705	2.0610356	5.6279745	7
TACAC	215190	1.8818189	9.6463585	5
CACAT	209745	1.8342029	5.5767426	7
GCTTT	205225	1.8044717	5.2889175	1
GAGAG	128530	1.8025308	6.0393505	7
GCCAA	124460	1.6460835	5.260914	1
TGGCT	122540	1.629536	5.3524814	6
ATACA	259925	1.5662013	5.1399193	6
TTGAG	166190	1.5227715	5.5677795	9
CCATG	110155	1.439608	5.380825	9
GAGTC	105900	1.4251615	7.5379148	9
AAGAC	154920	1.4118004	5.8661604	5
GTGTA	151005	1.383634	10.832051	1
GTCTT	152285	1.3389888	6.811614	1
GACTT	146860	1.3067886	6.6238313	7
ATGAG	140210	1.3001422	5.2658424	7
TATGA	211480	1.2966264	6.290325	4
TATAC	216140	1.2869234	6.1433067	5
TCATA	214645	1.2780219	6.5331283	2
TGAGT	139295	1.2763371	5.514509	8
ACTCA	145465	1.2720795	5.229065	8
GATTG	137120	1.256408	6.216511	7
GTGTT	137310	1.2432258	6.1182084	1
AACAC	137710	1.2187182	5.1815805	5
GTTCT	137665	1.2104403	5.143899	1
TGGAC	85265	1.1474636	7.663963	5
ACACC	89160	1.1451545	5.269329	6
ACATG	124570	1.1217531	5.7645173	8
GTCCA	84905	1.1096176	8.203508	1
GGACT	78205	1.0524528	7.2897434	6
ACACT	119655	1.0463731	5.4027658	6
AGACT	112535	1.0133779	5.5678167	6
GAGTA	105710	0.98022985	5.555257	1
GTATG	105610	0.96768713	5.55583	3
CATAC	106705	0.9331265	5.476348	3
ATACT	131455	0.78269875	5.2262125	6
GTATA	125315	0.7683315	7.7150884	1
>>END_MODULE