##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-157_CAGAGAGG-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1066943	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38006247756441	34.0	31.0	34.0	31.0	34.0
2	32.56220154216298	34.0	31.0	34.0	31.0	34.0
3	32.670062037053526	34.0	31.0	34.0	31.0	34.0
4	36.19966202505663	37.0	35.0	37.0	35.0	37.0
5	35.912186499185054	37.0	35.0	37.0	35.0	37.0
6	36.01781444744471	37.0	35.0	37.0	35.0	37.0
7	36.01181225238837	37.0	35.0	37.0	35.0	37.0
8	36.03353506232292	37.0	35.0	37.0	35.0	37.0
9	37.88199275875093	39.0	38.0	39.0	35.0	39.0
10-14	38.04141345882582	39.2	38.0	39.4	35.2	39.4
15-19	39.15464012604235	40.0	39.0	41.0	36.0	41.0
20-24	39.07706034905332	40.0	39.0	41.0	36.0	41.0
25-29	38.87565839974582	40.0	38.0	41.0	35.2	41.0
30-34	38.66445011589185	40.0	38.0	41.0	34.6	41.0
35-39	38.4235798913344	40.0	38.0	41.0	34.0	41.0
40-44	38.17636199871971	40.0	38.0	41.0	33.4	41.0
45-49	37.990574379324855	40.0	37.6	41.0	33.0	41.0
50-54	37.929384418849	40.0	37.0	41.0	33.0	41.0
55-59	37.53466605057627	39.4	36.4	41.0	32.6	41.0
60-64	36.97136585553305	38.8	35.2	40.4	31.4	41.0
65-69	36.215483301357246	37.6	35.0	40.0	30.8	41.0
70-74	35.64416374632947	36.4	34.6	39.0	30.6	40.8
75-79	34.4995953860703	35.2	33.4	37.4	29.4	39.2
80-84	34.33016590389552	35.0	34.0	36.4	30.0	38.2
85-89	33.69816925552724	35.0	34.0	35.6	29.8	36.8
90-94	33.27674861731133	35.0	34.0	35.0	29.0	36.0
95-99	32.9938245998146	35.0	33.0	35.0	29.0	35.4
100-101	32.6346515230898	35.0	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	17.0
15	42.0
16	105.0
17	200.0
18	338.0
19	566.0
20	886.0
21	1275.0
22	1799.0
23	2493.0
24	3441.0
25	4433.0
26	6017.0
27	7558.0
28	9610.0
29	12317.0
30	15585.0
31	19621.0
32	25782.0
33	34990.0
34	50065.0
35	78969.0
36	141715.0
37	262805.0
38	322110.0
39	64133.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52424262589473	23.145285174559465	10.76018119056032	24.570291008985485
2	15.450173882884489	23.40566736344804	39.86061248019797	21.283546273469504
3	15.939464432495457	27.391528882048995	33.036066593997994	23.63294009145756
4	11.266112622698682	18.23396376376245	44.12166348155431	26.37826013198456
5	10.400930508940027	39.587306913302776	39.12842579219321	10.883336785563989
6	25.606710011687596	44.309864725669506	19.146102462830722	10.937322799812174
7	23.819079369750774	33.98447714638927	26.620259938909573	15.576183544950387
8	21.120715914533392	38.80788383259462	24.028181449243306	16.043218803628683
9	22.966081599485634	14.464690241184394	24.73271768032594	37.83651047900403
10-14	20.443959986615965	25.697080350121798	33.06435301604678	20.794606647215456
15-19	19.771486928318982	31.68839937750508	28.553055470056023	19.987058224119917
20-24	19.7334344079636	30.020121199578902	30.26210421184283	19.984340180614666
25-29	19.781506413351092	30.506253052470143	29.70875333603935	20.00348719813941
30-34	19.626073090707447	30.248944671504987	29.838998432426173	20.285983805361397
35-39	19.79421590171331	30.51243304712515	29.684019561622527	20.00933148953902
40-44	19.477165835154707	30.275949943492698	30.188103871416157	20.05878034993644
45-49	19.690760821252	30.097650924015397	30.130415033306114	20.08117322142649
50-54	19.418750675164738	29.795657640464274	30.486463697142103	20.299127987228886
55-59	19.599002503866817	29.756053011766227	30.527334754541542	20.11760972982542
60-64	19.206177376775422	29.479543403453732	30.718878493735524	20.595400726035322
65-69	19.595880832722447	29.655877790961245	30.151129774247686	20.597111602068622
70-74	19.435989540559575	29.556561041864942	30.118037060941482	20.889412356634
75-79	19.76983582919668	29.921042277182643	29.556855615209837	20.752266278410843
80-84	19.725293099673383	29.957332215620585	29.251088720198908	21.066285964507124
85-89	19.90367646810717	29.896774256437595	29.165612214177767	21.03393706127747
90-94	19.759181322221377	29.52149880224266	29.58124138985966	21.138078485676306
95-99	19.96785509825115	29.487323683864226	29.22248628480443	21.322334933080192
100-101	19.756369137216456	28.72236728894354	30.152013886415567	21.369249687424432
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.09453363488022
2	36.73372015635399
3	39.57240452395301
4	37.64437275468324
5	21.284267294504016
6	36.54403281149977
7	39.39526291470116
8	37.163934718162075
9	60.80259207848967
10-14	41.238566633831425
15-19	39.7585451524389
20-24	39.71777458857827
25-29	39.784993611490506
30-34	39.91205689606884
35-39	39.80354739125233
40-44	39.53594618509115
45-49	39.77193404267849
50-54	39.71787866239362
55-59	39.71661223369223
60-64	39.801578102810744
65-69	40.19299243479107
70-74	40.32540189719357
75-79	40.52210210760752
80-84	40.7915790641805
85-89	40.937613529384635
90-94	40.897259807897676
95-99	41.29019003133134
100-101	41.12561882464089
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	4.0
7	6.5
8	6.5
9	8.5
10	19.5
11	32.5
12	50.5
13	78.0
14	131.0
15	203.5
16	309.0
17	463.5
18	694.0
19	989.0
20	1401.0
21	1984.5
22	2814.0
23	4011.5
24	5397.5
25	7041.5
26	9131.0
27	11430.0
28	14178.0
29	17375.0
30	20672.0
31	24336.5
32	28189.0
33	32331.5
34	37512.0
35	43435.5
36	50319.0
37	56985.5
38	61206.0
39	62335.5
40	60037.5
41	55875.5
42	51309.5
43	47018.0
44	43218.0
45	39415.0
46	36092.0
47	32901.0
48	29139.5
49	24992.5
50	21557.0
51	19234.5
52	16922.0
53	14811.0
54	12970.5
55	11267.5
56	10058.5
57	8620.5
58	7026.0
59	5919.0
60	4881.5
61	3980.5
62	3258.0
63	2305.5
64	1559.5
65	1269.0
66	1046.5
67	798.0
68	646.5
69	642.0
70	647.0
71	539.0
72	479.0
73	426.5
74	330.0
75	237.5
76	149.5
77	97.5
78	60.5
79	40.5
80	31.5
81	18.0
82	9.0
83	7.5
84	5.0
85	3.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012465520651056336
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.934926233172718E-4
20-24	8.060411849555225E-4
25-29	0.017245532329280944
30-34	0.006692016349514453
35-39	0.04185790618617864
40-44	0.04988082774806152
45-49	0.04988082774806152
50-54	0.050293220912457366
55-59	0.04054574611764639
60-64	0.05141792954262786
65-69	0.04270143765880652
70-74	0.003824009342579688
75-79	0.001649572657583395
80-84	2.0619658219792438E-4
85-89	0.0036178127603817634
90-94	0.00303671330146034
95-99	0.002268162404177168
100-101	9.37257191808747E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1066943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.481145497500417
#Duplication Level	Relative count
1	100.0
2	6.700399467376831
3	2.747743749075307
4	1.824530255955023
5	1.3439857967154905
6	0.9066429945258174
7	0.7344281698476106
8	0.571682201509099
9	0.474034620505992
10++	3.0572569906790945
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	289390	2.710789	5.2093215	5
GAGAG	165345	2.3251562	5.9317923	7
CAAAG	250385	2.2609246	5.0873256	4
ACACA	248935	2.1668525	7.8203177	6
CTTTG	242535	2.153357	9.285331	9
TACAC	216930	1.8776629	10.348959	5
GCTTT	205490	1.8244513	5.342811	1
CACAT	209575	1.8140007	5.5437856	7
TGGCT	122740	1.6452125	5.277127	6
TTGAG	167940	1.5555198	5.4204364	9
ATACA	259245	1.5505791	5.170594	6
GACTC	113950	1.4806801	5.884362	7
GAGTC	107240	1.4455665	7.2872176	9
CCATG	108780	1.4135004	5.644955	9
AAGAC	154815	1.3979473	6.247515	5
GTCTT	157425	1.3977042	6.264134	1
CATGA	153470	1.3780211	5.010872	9
GACTT	148095	1.3222921	7.0260477	7
TATGA	212735	1.3125366	6.311906	4
TGAGT	139460	1.291728	5.0701513	8
TATAC	216490	1.287585	6.5170665	5
TCATA	214375	1.2750059	6.35921	2
GTGTT	137665	1.2679425	6.283861	1
ACTCA	145915	1.2629843	5.3003793	8
GATTG	135085	1.2512052	6.3006396	7
AACAC	137935	1.200654	5.148833	5
GTGTA	129310	1.197715	10.828669	1
GTATG	124460	1.1527927	5.6629415	3
TGGAC	84635	1.1408572	7.947315	5
ACACC	89810	1.1313126	5.5761476	6
ACATG	125305	1.125125	5.6900167	8
GTCCA	85635	1.1127516	7.8320336	1
GGACT	78490	1.0580243	7.810067	6
ACACT	121500	1.0516573	5.7746015	6
AGACT	112520	1.0103273	5.833682	6
GAGTA	106080	0.9880986	5.343281	1
CATAC	105830	0.91602385	5.472442	3
GTATA	125205	0.7724923	7.356775	1
ATACT	129675	0.7712485	5.3289995	6
>>END_MODULE