##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-155_CAGAGAGG-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1297177	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.264120470837828	31.0	31.0	34.0	28.0	34.0
2	31.450396514893495	33.0	31.0	34.0	28.0	34.0
3	31.58394960749381	33.0	31.0	34.0	28.0	34.0
4	35.12986508394768	37.0	35.0	37.0	32.0	37.0
5	34.99473240737386	37.0	35.0	37.0	32.0	37.0
6	35.15469901177711	37.0	35.0	37.0	32.0	37.0
7	35.15585845262443	37.0	35.0	37.0	32.0	37.0
8	35.204186475708404	37.0	35.0	37.0	32.0	37.0
9	36.933823988553605	39.0	37.0	39.0	33.0	39.0
10-14	37.02060613162275	39.2	37.2	39.4	32.2	39.4
15-19	37.996811691850844	40.0	38.0	41.0	32.6	41.0
20-24	37.87518372589092	40.0	38.0	41.0	32.0	41.0
25-29	37.66346319738941	40.0	37.6	41.0	31.8	41.0
30-34	37.3715096706155	40.0	37.0	41.0	31.0	41.0
35-39	37.034825162641646	39.8	36.6	41.0	30.0	41.0
40-44	36.96433809726814	39.8	36.4	41.0	30.0	41.0
45-49	36.7688517449816	39.4	36.0	41.0	29.8	41.0
50-54	36.143775598858134	38.6	35.0	39.8	29.2	40.6
55-59	36.797871531795586	39.0	35.6	41.0	29.8	41.0
60-64	36.331611645904914	38.8	35.0	40.8	29.0	41.0
65-69	35.65599297551529	37.6	34.4	40.0	28.2	41.0
70-74	34.84056639918839	36.4	34.0	39.2	27.8	40.8
75-79	33.913625973941876	35.2	33.4	37.6	26.2	39.4
80-84	33.03330493833918	35.0	32.8	36.4	26.0	38.0
85-89	32.29398624859984	35.0	32.0	35.4	25.0	36.6
90-94	31.745669249454778	34.6	31.6	35.0	24.2	36.0
95-99	31.29673807044066	34.0	31.0	35.0	22.2	35.2
100-101	30.480098321200575	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	44.0
7	140.0
8	278.0
9	448.0
10	530.0
11	802.0
12	1406.0
13	2156.0
14	2846.0
15	3298.0
16	3728.0
17	4313.0
18	4672.0
19	5281.0
20	5890.0
21	6496.0
22	7189.0
23	8227.0
24	9152.0
25	10552.0
26	11988.0
27	13935.0
28	16703.0
29	19317.0
30	23901.0
31	29632.0
32	37240.0
33	49819.0
34	69671.0
35	104286.0
36	172252.0
37	292757.0
38	319448.0
39	58725.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92348889055113	23.533154068657804	10.321110998867193	24.222246041923874
2	14.952895552818903	23.683084271348143	40.36940188880466	20.994618287028292
3	15.508785890757002	28.23182599093806	32.963437616628575	23.295950501676366
4	10.788891107905103	18.252702080623283	44.990365271488066	25.968041539983545
5	10.469706328477493	39.56295125884011	39.09325264171735	10.874089770965053
6	25.368844813045133	44.320506831087656	19.49184293663532	10.81880541923189
7	23.474255494653747	34.16861321183808	27.165367685037427	15.19176360847075
8	20.90828634556172	38.93278011881469	24.312297921745124	15.846635613878469
9	22.88433830235181	14.778159548741069	25.217877982162722	37.1196241667444
10-14	20.21180045653004	26.038263532920798	33.21280701830129	20.537128992247872
15-19	19.510563406346925	31.969340582874995	28.87119787695519	19.648898133822886
20-24	19.515244397988695	30.350806363313637	30.49087785019954	19.643071388498132
25-29	19.470329333497386	30.79344829893647	30.058855037323273	19.677367330242873
30-34	19.365331149271885	30.582200186993813	30.129918147034374	19.92255051669993
35-39	19.533492498356335	30.833402204791234	29.99784750995758	19.63525778689485
40-44	19.25897461321048	30.704101642763508	30.356467946658956	19.68045579736706
45-49	19.462047323158014	30.556409615124263	30.312522068916387	19.669020992801332
50-54	19.280718679983845	30.255828450817717	30.63161729979399	19.831835569404447
55-59	19.46849030604386	30.237776108105923	30.69837837104409	19.59535521480613
60-64	19.190231897600533	30.03753166830887	30.877121564672738	19.895114869417856
65-69	19.457731705677244	30.2355715763431	30.474942743983412	19.83175397399624
70-74	19.487661808741922	30.065206174199982	30.487911320590033	19.959220696468066
75-79	19.65990143420297	30.426461595204252	30.16630858107131	19.74732838952147
80-84	19.663802960131438	30.420304123937868	30.02526527770603	19.89062763822466
85-89	19.793450821021963	30.441170937740736	29.935753481857212	19.829624759380092
90-94	19.787369571194173	30.182461937046924	30.28884191386506	19.74132657789384
95-99	20.026012694873558	30.200862104191096	29.872195482240976	19.900929718694368
100-101	20.107640618181875	29.633588621950295	30.555803974114443	19.702966785753382
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.145734932475
2	35.9475138398472
3	38.80473639243337
4	36.756932647888654
5	21.343796099442546
6	36.18765023227702
7	38.66601910312449
8	36.75492195944018
9	60.00396246909621
10-14	40.74892944877791
15-19	39.159461540169815
20-24	39.158315786486824
25-29	39.147696663740255
30-34	39.28788166597181
35-39	39.168750285251186
40-44	38.93943041057754
45-49	39.13106831595935
50-54	39.112554249388296
55-59	39.06384552084999
60-64	39.08534676701839
65-69	39.28948567967348
70-74	39.446882505209985
75-79	39.407229823724435
80-84	39.5544305983561
85-89	39.62307558040205
90-94	39.52869614908801
95-99	39.92694241356792
100-101	39.81060740393526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.0
2	3.0
3	5.5
4	8.5
5	11.0
6	21.0
7	28.5
8	32.0
9	45.5
10	58.0
11	79.5
12	120.5
13	180.5
14	258.0
15	379.0
16	584.5
17	818.0
18	1157.5
19	1637.5
20	2380.5
21	3502.5
22	4882.0
23	6663.5
24	8775.5
25	11279.5
26	14336.0
27	17711.5
28	21493.5
29	25546.5
30	30035.5
31	34631.5
32	39155.5
33	44369.5
34	50230.0
35	56517.0
36	63092.0
37	68705.5
38	72194.5
39	72385.0
40	69228.5
41	65125.0
42	59960.0
43	54515.5
44	49972.0
45	45286.0
46	40919.5
47	36481.0
48	31627.5
49	27049.0
50	23096.0
51	20226.5
52	18010.0
53	15836.5
54	13781.5
55	11920.5
56	10409.5
57	9164.5
58	7788.0
59	6373.5
60	5210.5
61	4322.0
62	3523.5
63	2613.0
64	1892.5
65	1530.0
66	1249.5
67	975.5
68	831.0
69	808.5
70	786.0
71	693.0
72	571.0
73	481.5
74	390.0
75	300.0
76	218.0
77	157.0
78	122.5
79	90.0
80	73.0
81	50.0
82	40.0
83	31.0
84	21.5
85	21.5
86	15.0
87	8.5
88	6.0
89	5.0
90	5.0
91	8.0
92	7.0
93	4.0
94	6.5
95	6.0
96	4.5
97	4.0
98	2.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03068201178405106
2	0.029140202146661562
3	0.025054406607579384
4	0.023589687452059357
5	0.02991110696535631
6	0.0014647191555200254
7	5.396333730863252E-4
8	0.0023898049379537257
9	4.6254289121685013E-4
10-14	0.006799380500887697
15-19	0.02486938945109264
20-24	0.02064483104464541
25-29	0.0018347534684935057
30-34	0.0016805725047545554
35-39	0.0057509499474628365
40-44	0.013845450543757715
45-49	0.008001992018051508
50-54	0.005057135610637561
55-59	0.0037928517079781707
60-64	0.010715576979857028
65-69	0.015063480157295419
70-74	0.018856331865273593
75-79	0.007261923392104548
80-84	0.010792667461726503
85-89	0.008526207294763936
90-94	0.009898417872040594
95-99	0.009096676860598053
100-101	0.006398509995166428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1297177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.090862422997944
#Duplication Level	Relative count
1	100.0
2	6.861224709749657
3	2.718982581985618
4	1.8789181550915808
5	1.4088892348408188
6	1.0783720832759518
7	0.8418607157612373
8	0.6915712138721402
9	0.5760099634155834
10++	3.696762488698349
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTCTT	291600	2.1109967	5.0428004	1
CTTTG	279725	2.0450711	8.356706	9
ACACA	248350	1.8228959	7.0620813	6
GAGAG	153950	1.764094	5.8903975	7
GCTTT	235935	1.7249222	5.170731	1
CTCCA	151940	1.6826108	5.1785226	1
TACAC	204215	1.4920595	8.785785	5
GACTC	125745	1.4063046	5.1198516	7
CCATG	123045	1.3761083	5.212072	9
GAGTC	120635	1.3625079	6.4467463	9
AAGAC	176945	1.3116353	5.5696993	5
GTCTT	176115	1.2875777	6.5077443	1
GTGTT	165700	1.2234232	6.0850596	1
GACTT	165530	1.2157747	6.0373	7
GATTG	163680	1.214085	5.528713	7
GTTCT	165585	1.2105931	5.6211314	1
TATGA	247310	1.1984087	5.5167327	4
TCATA	248810	1.1938617	5.7211103	2
GTGTA	154295	1.1444725	9.930699	1
TGGAC	98090	1.1078742	7.3084807	5
GTCCA	97470	1.090083	7.7687097	1
TATAC	219065	1.0511367	6.158594	5
ACACT	140545	1.0268663	5.201372	6
GGACT	90350	1.0204551	6.857463	6
AGACT	129890	0.95840997	5.1705503	6
GTATG	127285	0.9441276	5.037136	3
ATACT	158845	0.7621839	5.242528	6
GTATA	154450	0.7484301	7.517251	1
>>END_MODULE