FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | Nextera-155_CAGAGAGG-TATCCTCT_L005_R1_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1297177 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 39 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
![[WARN]](Icons/warning.png)
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CAAAG | 285795 | 2.1233141 | 5.0144362 | 4 |
| GAGAG | 180605 | 2.117398 | 5.7755017 | 7 |
| AGAGA | 272955 | 2.0781069 | 5.046248 | 8 |
| CTTTG | 278415 | 2.0317633 | 8.44256 | 9 |
| ACACA | 248400 | 1.8009181 | 7.0658054 | 6 |
| TTGAG | 196575 | 1.4788326 | 5.208854 | 9 |
| TACAC | 204030 | 1.4704264 | 8.767448 | 5 |
| CCATG | 125270 | 1.3902482 | 5.99451 | 9 |
| GACTC | 125090 | 1.3882505 | 5.0904903 | 7 |
| GAGTC | 119880 | 1.3633558 | 6.3193197 | 9 |
| GTCTT | 180865 | 1.3198816 | 6.2318687 | 1 |
| AAGAC | 176100 | 1.308335 | 5.6016145 | 5 |
| ATACA | 272040 | 1.3046782 | 5.0856056 | 6 |
| GATTG | 164880 | 1.2403914 | 6.091587 | 7 |
| GTGTT | 165465 | 1.237382 | 5.83851 | 1 |
| GTTCT | 167585 | 1.2229694 | 5.357779 | 1 |
| TATGA | 248845 | 1.2156924 | 5.7773657 | 4 |
| AGAAC | 163105 | 1.2117887 | 5.039652 | 5 |
| GACTT | 164070 | 1.2044885 | 5.816326 | 7 |
| TCATA | 251980 | 1.2012783 | 5.622372 | 2 |
| ACCAT | 159420 | 1.1489263 | 5.000486 | 8 |
| TGGAC | 98220 | 1.1170237 | 7.2953405 | 5 |
| ACATG | 150490 | 1.1114098 | 5.0705266 | 8 |
| GTCCA | 100065 | 1.1105226 | 7.2276936 | 1 |
| GTATG | 141525 | 1.0646917 | 5.500666 | 3 |
| GTGTA | 140715 | 1.0585982 | 10.032333 | 1 |
| TATAC | 218870 | 1.0434312 | 6.4098916 | 5 |
| ACACT | 141125 | 1.0170758 | 5.129779 | 6 |
| GGACT | 88470 | 1.0061402 | 6.572975 | 6 |
| AGACT | 130165 | 0.96130407 | 5.160048 | 6 |
| GAGTA | 125320 | 0.94842756 | 5.0498757 | 1 |
| ATACT | 159700 | 0.7613467 | 5.256435 | 6 |
| GTATA | 153745 | 0.75109655 | 7.173467 | 1 |