##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-155_CAGAGAGG-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1297177	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37247885215356	33.0	31.0	34.0	31.0	34.0
2	32.55535366414915	34.0	31.0	34.0	31.0	34.0
3	32.65934024423807	34.0	31.0	34.0	31.0	34.0
4	36.19319722751791	37.0	35.0	37.0	35.0	37.0
5	35.907044296961786	37.0	35.0	37.0	35.0	37.0
6	36.01357177933312	37.0	35.0	37.0	35.0	37.0
7	36.013224872164706	37.0	35.0	37.0	35.0	37.0
8	36.03781056864252	37.0	35.0	37.0	35.0	37.0
9	37.8715441300609	39.0	38.0	39.0	35.0	39.0
10-14	38.04071271692298	39.2	38.0	39.4	35.2	39.4
15-19	39.151199258081206	40.0	39.0	41.0	36.0	41.0
20-24	39.078747618867745	40.0	39.0	41.0	36.0	41.0
25-29	38.87029927295966	40.0	38.0	41.0	35.2	41.0
30-34	38.66343128192991	40.0	38.0	41.0	34.8	41.0
35-39	38.42519054839856	40.0	38.0	41.0	34.0	41.0
40-44	38.176175032397275	40.0	38.0	41.0	33.4	41.0
45-49	37.99173559198167	40.0	37.6	41.0	33.0	41.0
50-54	37.935532776174725	40.0	37.0	41.0	33.0	41.0
55-59	37.54137962668163	39.4	36.4	41.0	32.6	41.0
60-64	36.97945122369576	38.8	35.4	40.4	31.4	41.0
65-69	36.23446252901493	37.6	35.0	40.0	30.8	41.0
70-74	35.67483281001745	36.6	34.6	39.0	30.6	40.8
75-79	34.55692492235061	35.2	33.6	37.4	29.4	39.2
80-84	34.401323026849845	35.0	34.0	36.6	30.2	38.4
85-89	33.762695299099505	35.0	34.0	35.6	30.0	36.8
90-94	33.33098089158226	35.0	34.0	35.0	29.0	36.0
95-99	33.04176638962917	35.0	33.4	35.0	29.0	35.6
100-101	32.68850434443411	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	13.0
15	48.0
16	108.0
17	223.0
18	382.0
19	647.0
20	1005.0
21	1473.0
22	2118.0
23	3026.0
24	3922.0
25	5289.0
26	6832.0
27	9037.0
28	11611.0
29	14691.0
30	18572.0
31	23980.0
32	31159.0
33	42342.0
34	60568.0
35	96366.0
36	172987.0
37	321369.0
38	391049.0
39	78280.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.25766953931499	23.627230516729792	10.764914888253491	24.35018505570173
2	15.28073757220442	23.503329190477807	40.11743916016197	21.098494077155802
3	15.81696252708767	27.807384805620206	33.3010838150846	23.074568852207523
4	11.119376923889337	18.25733882114777	44.569862092836985	26.053422162125912
5	10.36597164457896	39.70660904410116	39.12102974382062	10.80638956749927
6	25.30626121184696	44.530160494674206	19.36482068368465	10.798757609794192
7	23.39958232376923	34.20820751524272	27.151113533465367	15.24109662752269
8	20.940395952132977	39.006473287762574	24.168097337526028	15.885033422578415
9	22.710239234892384	14.620826610400893	25.241196845149123	37.4277373095576
10-14	20.158343849759902	25.949164994445628	33.241076583997405	20.651414571797062
15-19	19.45295403801865	31.975555276999028	28.864304990412155	19.707185694570168
20-24	19.437462986624226	30.281115866262937	30.538122254495992	19.743298892616846
25-29	19.44097394312312	30.76735894044147	30.1094526381173	19.682214478318112
30-34	19.342715838084334	30.506601289805296	30.211603621939798	19.939079250170575
35-39	19.491639144708227	30.751462062268214	30.078472286061377	19.67842650696219
40-44	19.244544113096566	30.637991464817592	30.38919518591772	19.72826923616812
45-49	19.44103327228445	30.495529466280146	30.371693761768668	19.69174349966673
50-54	19.232524167760584	30.227447665441375	30.70452473541566	19.835503431382378
55-59	19.407918684640773	30.192028873739083	30.715673874818766	19.68437856680137
60-64	19.09718344360686	29.9804031303161	30.915866738818032	20.00654668725901
65-69	19.352262340353793	30.208574660640465	30.4823265890738	19.956836409931945
70-74	19.277976892115152	30.02523257059993	30.48146951777497	20.21532101950995
75-79	19.47963122428511	30.401701567350752	30.101922897536692	20.01674431082745
80-84	19.444090255852988	30.411804239768493	29.893338953753794	20.250766550624718
85-89	19.571870158576658	30.387686996121925	29.781815243884385	20.258627601417025
90-94	19.44869394729287	30.042542654438375	30.20110624469797	20.30765715357078
95-99	19.594770135332055	30.090948604101513	29.84943455276035	20.464846707806082
100-101	19.49678358865088	29.439925437999715	30.659922269577244	20.403368703772156
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.60785459501672
2	36.37923164936022
3	38.89153137929519
4	37.172799086015246
5	21.17236121207823
6	36.10501882164115
7	38.640678951291925
8	36.82542937471139
9	60.13797654444999
10-14	40.809758421556964
15-19	39.16013973258882
20-24	39.180761879241075
25-29	39.12318842144123
30-34	39.2817950882549
35-39	39.17006565167041
40-44	38.97281334926468
45-49	39.132776771951185
50-54	39.068027599142965
55-59	39.09229725144214
60-64	39.10373013086587
65-69	39.309098750285735
70-74	39.4932979116251
75-79	39.49637553511256
80-84	39.69485680647771
85-89	39.83049775999368
90-94	39.756351100863654
95-99	40.05961684313814
100-101	39.90015229242304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.5
7	5.5
8	14.5
9	22.0
10	34.5
11	51.5
12	98.5
13	183.5
14	263.5
15	377.0
16	558.5
17	825.5
18	1227.5
19	1758.0
20	2531.0
21	3608.0
22	5093.5
23	7015.0
24	9122.0
25	11447.0
26	14375.0
27	17663.0
28	21402.0
29	25368.0
30	29456.0
31	34112.0
32	38744.0
33	43776.5
34	49758.0
35	56050.5
36	62533.5
37	68484.0
38	71719.0
39	71712.0
40	69052.5
41	64754.0
42	59128.5
43	53797.5
44	49472.5
45	45006.0
46	41231.5
47	37250.0
48	32569.5
49	27741.0
50	23705.0
51	20957.0
52	18577.5
53	16487.0
54	14527.0
55	12401.0
56	10777.0
57	9380.5
58	7904.0
59	6502.0
60	5261.5
61	4401.5
62	3558.5
63	2623.5
64	1845.0
65	1421.5
66	1149.0
67	897.0
68	737.5
69	773.5
70	783.5
71	654.5
72	576.0
73	515.5
74	402.0
75	285.5
76	217.5
77	164.5
78	97.0
79	54.0
80	44.5
81	32.5
82	18.0
83	14.5
84	9.5
85	9.0
86	7.5
87	4.0
88	1.5
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01271992950846338
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010484305534248601
20-24	8.325772041903302E-4
25-29	0.01651278121644155
30-34	0.006321419513296953
35-39	0.039732434355527424
40-44	0.04734897396423156
45-49	0.0478269349518223
50-54	0.04741064634972714
55-59	0.03831396948912908
60-64	0.04960001603482023
65-69	0.04075002871620449
70-74	0.003391981202256901
75-79	0.0015418096373895002
80-84	1.8501715648674006E-4
85-89	0.0029140202146661557
90-94	0.0024514773234493056
95-99	0.0020660249141019305
100-101	1.1563572280421253E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1297177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.44426363967315
#Duplication Level	Relative count
1	100.0
2	7.31704368221881
3	2.775930650094909
4	1.949559308919007
5	1.4496258055155904
6	1.1957297094703243
7	0.8964950248455466
8	0.7266264463009757
9	0.6063280579366711
10++	3.9855641933962835
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	285795	2.1233141	5.0144362	4
GAGAG	180605	2.117398	5.7755017	7
AGAGA	272955	2.0781069	5.046248	8
CTTTG	278415	2.0317633	8.44256	9
ACACA	248400	1.8009181	7.0658054	6
TTGAG	196575	1.4788326	5.208854	9
TACAC	204030	1.4704264	8.767448	5
CCATG	125270	1.3902482	5.99451	9
GACTC	125090	1.3882505	5.0904903	7
GAGTC	119880	1.3633558	6.3193197	9
GTCTT	180865	1.3198816	6.2318687	1
AAGAC	176100	1.308335	5.6016145	5
ATACA	272040	1.3046782	5.0856056	6
GATTG	164880	1.2403914	6.091587	7
GTGTT	165465	1.237382	5.83851	1
GTTCT	167585	1.2229694	5.357779	1
TATGA	248845	1.2156924	5.7773657	4
AGAAC	163105	1.2117887	5.039652	5
GACTT	164070	1.2044885	5.816326	7
TCATA	251980	1.2012783	5.622372	2
ACCAT	159420	1.1489263	5.000486	8
TGGAC	98220	1.1170237	7.2953405	5
ACATG	150490	1.1114098	5.0705266	8
GTCCA	100065	1.1105226	7.2276936	1
GTATG	141525	1.0646917	5.500666	3
GTGTA	140715	1.0585982	10.032333	1
TATAC	218870	1.0434312	6.4098916	5
ACACT	141125	1.0170758	5.129779	6
GGACT	88470	1.0061402	6.572975	6
AGACT	130165	0.96130407	5.160048	6
GAGTA	125320	0.94842756	5.0498757	1
ATACT	159700	0.7613467	5.256435	6
GTATA	153745	0.75109655	7.173467	1
>>END_MODULE