##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-154_CAGAGAGG-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1786736	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46574423977577	31.0	31.0	34.0	30.0	34.0
2	31.652753960294078	33.0	31.0	34.0	30.0	34.0
3	31.794382606048124	33.0	31.0	34.0	30.0	34.0
4	35.33870364732115	37.0	35.0	37.0	33.0	37.0
5	35.20674962613391	37.0	35.0	37.0	32.0	37.0
6	35.364747226227045	37.0	35.0	37.0	32.0	37.0
7	35.370175000671615	37.0	35.0	37.0	33.0	37.0
8	35.419213023076715	37.0	35.0	37.0	33.0	37.0
9	37.18362813532609	39.0	37.0	39.0	34.0	39.0
10-14	37.27688947891574	39.2	37.2	39.4	33.0	39.4
15-19	38.28489233999875	40.0	38.0	41.0	33.0	41.0
20-24	38.167937960616456	40.0	38.0	41.0	33.0	41.0
25-29	37.965829758845175	40.0	38.0	41.0	32.8	41.0
30-34	37.68284279266775	40.0	37.6	41.0	32.0	41.0
35-39	37.353560794655735	40.0	37.0	41.0	31.0	41.0
40-44	37.29152129917346	40.0	37.0	41.0	30.8	41.0
45-49	37.107283224830084	39.8	36.4	41.0	30.4	41.0
50-54	36.45915602528857	38.6	35.4	40.0	30.0	40.6
55-59	37.105757761639104	39.6	36.0	41.0	31.0	41.0
60-64	36.64431029542138	39.0	35.0	41.0	30.0	41.0
65-69	35.95879883765705	37.6	35.0	40.0	29.0	41.0
70-74	35.1298467149036	36.4	34.0	39.2	28.8	40.8
75-79	34.19672307492545	35.2	33.8	37.6	27.8	39.4
80-84	33.29711720142204	35.0	33.0	36.4	26.4	38.0
85-89	32.55332035622498	35.0	32.6	35.4	25.8	36.6
90-94	32.007536423959664	35.0	32.0	35.0	25.0	36.0
95-99	31.564563315453434	34.2	31.4	35.0	23.6	35.2
100-101	30.737872298985412	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	50.0
7	183.0
8	391.0
9	631.0
10	660.0
11	959.0
12	1623.0
13	2314.0
14	2905.0
15	3525.0
16	4037.0
17	4497.0
18	5071.0
19	5617.0
20	6420.0
21	7191.0
22	8139.0
23	9470.0
24	10932.0
25	12513.0
26	14701.0
27	17225.0
28	20407.0
29	24617.0
30	30166.0
31	38314.0
32	49355.0
33	65666.0
34	93400.0
35	141191.0
36	239290.0
37	417628.0
38	465122.0
39	82456.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.204695401346406	23.207423150483066	10.23656169236902	24.351319755801505
2	15.057724618215799	23.588852690670954	40.195660730071836	21.15776196104141
3	15.643257829382721	27.870922954619253	32.68651118003382	23.799308035964213
4	10.925613144460188	18.394137863661328	44.642582224255044	26.037666767623442
5	10.564095501076078	39.41770859227508	39.00564129093519	11.012554615713649
6	25.394332536701945	44.12058051480671	19.44220877712418	11.042878171367166
7	23.883503187672208	34.01879526083167	26.7324554898426	15.365246061653515
8	21.05667909013009	38.78823937004701	24.254690141956587	15.900391397866315
9	23.066013553296425	14.648485160550816	24.830919604818654	37.454581681334105
10-14	20.456236077547842	25.782441951231633	33.14191495028022	20.619407020940308
15-19	19.79383250915154	31.6960207952229	28.61187229336169	19.898274402263862
20-24	19.700056092533647	29.978178772838675	30.380477559067902	19.941287575559777
25-29	19.712257129430895	30.700863786066364	29.671039066377624	19.91584001812511
30-34	19.49768791368211	30.34473649156153	29.838853572344064	20.318722022412295
35-39	19.80336507930111	30.67617153860198	29.603039391799246	19.91742399029766
40-44	19.400387602930792	30.491863439545554	30.109275993505396	19.998472964018262
45-49	19.684709877651617	30.30621127496571	29.988597146801492	20.02048170058118
50-54	19.528912160888442	29.861541155035663	30.440673192438627	20.16887349163726
55-59	19.75762817297395	29.9308687641598	30.516542656861706	19.794960406004545
60-64	19.389758193086173	29.51424234516558	30.85467728795898	20.241322173789268
65-69	19.813001015086805	29.80246502529432	30.28497551734218	20.09955844227669
70-74	19.648331712361212	29.595528736773645	30.397800777282907	20.358338773582233
75-79	19.884356387052804	30.092426229955976	29.906911055929715	20.116306327061505
80-84	19.90310184694892	30.074004682582313	29.736267220199807	20.28662625026896
85-89	20.085824725504953	30.11373628840135	29.562233001155175	20.238205984938524
90-94	20.00894678877227	29.74909138374537	30.098331835858218	20.14362999162414
95-99	20.398346904453547	29.763265048416415	29.495253289080033	20.343134758050006
100-101	20.38433315963545	28.99500007556138	30.49010789605217	20.130558868751002
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.55601515714791
2	36.2154865792572
3	39.442565865346936
4	36.96327991208363
5	21.57665011678973
6	36.43721070806911
7	39.24874924932572
8	36.957070487996404
9	60.52059523463053
10-14	41.07564309848815
15-19	39.6921069114154
20-24	39.64134366809343
25-29	39.628097147556005
30-34	39.8164099360944
35-39	39.72078906959877
40-44	39.398860566949054
45-49	39.70519157823279
50-54	39.6977856525257
55-59	39.552588578978494
60-64	39.631080366875445
65-69	39.9125594573635
70-74	40.006670485943445
75-79	40.00066271411431
80-84	40.18972809721788
85-89	40.32403071044347
90-94	40.152576780396416
95-99	40.74148166250355
100-101	40.51489202838645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	2.5
3	3.5
4	9.0
5	15.5
6	22.5
7	25.5
8	39.0
9	58.0
10	77.0
11	97.5
12	138.5
13	205.5
14	273.0
15	412.0
16	638.5
17	916.0
18	1260.0
19	1735.5
20	2504.5
21	3652.0
22	5085.5
23	6953.0
24	9469.5
25	12294.0
26	15871.5
27	20185.0
28	24815.0
29	29957.5
30	35605.5
31	41925.5
32	48527.0
33	55919.5
34	64933.5
35	75768.0
36	88587.0
37	100918.0
38	109024.0
39	109814.5
40	103739.0
41	95096.0
42	85223.0
43	75535.5
44	68136.5
45	61888.0
46	56129.0
47	50234.5
48	44036.0
49	37477.5
50	31919.5
51	28617.0
52	25438.5
53	22450.0
54	19856.5
55	17144.5
56	15058.0
57	13371.0
58	11608.0
59	9701.5
60	8075.5
61	6946.5
62	5777.0
63	4412.0
64	3375.5
65	2791.0
66	2351.5
67	1961.5
68	1608.5
69	1504.5
70	1469.0
71	1258.5
72	1066.0
73	889.5
74	698.5
75	544.0
76	394.0
77	291.0
78	219.0
79	158.5
80	116.0
81	87.5
82	62.5
83	46.5
84	40.5
85	28.0
86	25.0
87	20.0
88	14.5
89	16.0
90	13.5
91	11.0
92	11.0
93	11.0
94	7.5
95	6.0
96	8.5
97	6.5
98	4.5
99	5.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036267249330622996
2	0.03262933080208828
3	0.02887947631882942
4	0.02759221283950175
5	0.034700145964484966
6	0.002574526958655336
7	5.037117962586527E-4
8	0.003525982573810569
9	6.716157283448702E-4
10-14	0.008025807953721199
15-19	0.026842241942849978
20-24	0.022499126899553152
25-29	0.0024402038129863616
30-34	0.0021267831397587555
35-39	0.006458704587583168
40-44	0.015032998719452677
45-49	0.009268297051159209
50-54	0.005417700208648619
55-59	0.004264759874989926
60-64	0.01183163041434213
65-69	0.01610758388480447
70-74	0.02011489106392886
75-79	0.008037001549193613
80-84	0.011215982663359332
85-89	0.009212329073797137
90-94	0.00969365367911096
95-99	0.009660072892693716
100-101	0.006100509532465904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1786736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.91935420513022
#Duplication Level	Relative count
1	100.0
2	7.665714899757453
3	2.847318166467103
4	1.9158146817721287
5	1.4958091553836235
6	1.1869454422645913
7	0.9462405207086059
8	0.7829050382241871
9	0.6895704768045193
10++	5.2783150655491085
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1824	0.10208559070842027	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTTG	441055	2.3344233	10.041283	9
ACACA	397005	2.1119294	8.551838	6
GAGAG	252430	2.0735977	5.485513	7
GCTTT	367165	1.9433368	5.7667527	1
TACAC	342210	1.8088115	10.292133	5
GCCAA	222720	1.7784375	5.2835827	1
CACAT	326880	1.7277819	6.0878506	7
TGGCT	214585	1.7157937	5.5565395	6
GACTC	205225	1.6282712	6.179336	7
TTGAG	295455	1.5962951	6.060395	9
GGCTT	197370	1.5781447	5.046354	1
GAGTC	195460	1.5729194	9.093016	9
ATACA	419880	1.4996223	5.3432364	6
CCATG	187020	1.4838313	5.9408317	9
ATGGA	270990	1.4735266	5.0934777	4
AAGAC	273090	1.4734702	6.4360814	5
CATGA	271715	1.456687	5.568979	9
TCCAT	272615	1.4317508	5.324624	2
GTCTT	269090	1.424244	6.9529686	1
TGTGT	260930	1.4007574	5.31622	8
GACTT	261195	1.3913442	7.561226	7
TATGA	384805	1.3850536	6.919543	4
TCATA	385600	1.3683931	7.1716337	2
GTGTA	252935	1.3665665	11.30148	1
TGAGT	252425	1.3638111	6.2831774	8
ACTCA	255870	1.352446	5.521468	8
ATGAG	242845	1.3204862	5.75386	7
GTGTT	243605	1.3077513	6.205799	1
AACAC	242310	1.2890056	5.442363	5
GATTG	237275	1.281958	6.6472507	7
GTTCT	233635	1.2365873	5.317429	1
ACACC	153665	1.2097685	5.3335485	6
ACCAT	226320	1.1962543	5.0011067	8
TATAC	335795	1.1916481	6.2678976	5
TGGAC	147730	1.1888233	8.494444	5
GTCCA	149260	1.1842407	8.863908	1
ACATG	216530	1.1608355	6.2138224	8
ATGTG	210600	1.1378373	5.4053125	7
ACACT	212140	1.1213033	5.549463	6
GGACT	137900	1.1097187	8.101557	6
AGACT	194390	1.0421412	5.971812	6
GTATG	192535	1.040235	6.232395	3
TGTAT	287965	1.0298707	5.2757525	2
CATAC	188115	0.994315	5.981866	3
GAGTA	182560	0.99268234	5.1120276	1
ATACT	226020	0.8020855	5.305665	6
GTATA	217245	0.781944	7.588402	1
>>END_MODULE