##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-154_CAGAGAGG-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1786736	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34873310886443	33.0	31.0	34.0	31.0	34.0
2	32.53680622095262	34.0	31.0	34.0	31.0	34.0
3	32.64034753875223	34.0	31.0	34.0	31.0	34.0
4	36.17886526045258	37.0	35.0	37.0	35.0	37.0
5	35.89448860939725	37.0	35.0	37.0	35.0	37.0
6	35.99935692794011	37.0	35.0	37.0	35.0	37.0
7	35.99555278451881	37.0	35.0	37.0	35.0	37.0
8	36.01958991143627	37.0	35.0	37.0	35.0	37.0
9	37.85281541313322	39.0	38.0	39.0	35.0	39.0
10-14	38.012818569727145	39.2	37.8	39.4	35.2	39.4
15-19	39.11509120541591	40.0	38.6	41.0	36.0	41.0
20-24	39.03403255993051	40.0	38.8	41.0	36.0	41.0
25-29	38.82579306623923	40.0	38.0	41.0	35.0	41.0
30-34	38.613009980209725	40.0	38.0	41.0	34.6	41.0
35-39	38.36678737093784	40.0	38.0	41.0	34.0	41.0
40-44	38.11202595123175	40.0	38.0	41.0	33.2	41.0
45-49	37.92221570506219	40.0	37.0	41.0	33.0	41.0
50-54	37.86718989263103	40.0	37.0	41.0	33.0	41.0
55-59	37.47868134967897	39.4	36.2	41.0	32.4	41.0
60-64	36.90930568366004	38.8	35.2	40.2	31.4	41.0
65-69	36.16000438788942	37.6	35.0	40.0	30.6	41.0
70-74	35.5998069104781	36.4	34.4	39.0	30.4	40.8
75-79	34.470198395286154	35.2	33.4	37.4	29.4	39.2
80-84	34.31755782611422	35.0	34.0	36.4	30.0	38.2
85-89	33.69124750382821	35.0	34.0	35.6	29.8	36.8
90-94	33.25981958162818	35.0	34.0	35.0	29.0	36.0
95-99	32.97597809637237	35.0	33.0	35.0	29.0	35.4
100-101	32.60959201583222	34.5	32.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	38.0
15	68.0
16	145.0
17	341.0
18	647.0
19	915.0
20	1489.0
21	2087.0
22	2975.0
23	4224.0
24	5760.0
25	7685.0
26	9656.0
27	12839.0
28	15984.0
29	20808.0
30	26561.0
31	33452.0
32	44136.0
33	59278.0
34	85921.0
35	136228.0
36	243529.0
37	449275.0
38	527888.0
39	94719.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4190456788244	23.30875966007289	10.774619193882028	24.497575467220674
2	15.298196669189329	23.507561271601237	39.885203910276786	21.309038148932643
3	15.88124938435225	27.44042768489581	33.086197401294875	23.592125529457068
4	11.277323566548164	18.441504508780255	44.169032246509836	26.112139678161743
5	10.344169479990327	39.57366952924215	39.08865103742243	10.993509953345095
6	25.31705859175614	44.44327533558399	19.233003644634685	11.00666242802518
7	23.72891126612997	34.03866044004262	26.72448531848018	15.507942975347225
8	20.998009778725006	38.89063633351542	24.1655174575315	15.94583643022808
9	22.856034691191088	14.484176733440195	24.73627889066991	37.9235096846988
10-14	20.33332288597756	25.794566181013874	33.145780909994535	20.726330023014032
15-19	19.672592109354763	31.80288811880944	28.59979990073393	19.924719871101864
20-24	19.638677508998025	29.976070186954324	30.409197788221714	19.976054515825936
25-29	19.679474082238464	30.683331476753473	29.684540026275734	19.952654414732322
30-34	19.467902162453456	30.36606353856794	29.831735790533493	20.334298508445116
35-39	19.727641522142775	30.679203038632735	29.618494549146263	19.97466089007823
40-44	19.35387306593082	30.447624465807184	30.13670632609728	20.061796142164724
45-49	19.67274471072642	30.27766572467399	30.01381519577463	20.035774368824963
50-54	19.431590700508046	29.886334938721436	30.465730535579194	20.21634382519132
55-59	19.67932724184318	29.933731332787545	30.516408267127936	19.870533158241344
60-64	19.27524785829335	29.541810176933964	30.813092571317984	20.3698493934547
65-69	19.66683180912518	29.80448088528303	30.215851346702404	20.312835958889384
70-74	19.487454114239856	29.607110542354487	30.255480405004764	20.64995493840089
75-79	19.747374698985745	30.075579162453614	29.717345193348173	20.459700945212468
80-84	19.668801139420257	30.066503493638557	29.4984052459539	20.766290120987286
85-89	19.806122524367613	30.122370338463384	29.30225466884069	20.769252468328318
90-94	19.643879386135275	29.653490471861097	29.8759133385885	20.826716803415128
95-99	19.900397157658606	29.689908076136064	29.361413469331097	21.04828129687423
100-101	19.683388974861966	28.80334800627961	30.516780608204353	20.99648241065407
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.91662114604507
2	36.607234818121974
3	39.473374913809316
4	37.38946324470991
5	21.337679433335424
6	36.323721019781324
7	39.2368542414772
8	36.94384620895308
9	60.77954437588989
10-14	41.05965290899159
15-19	39.59731198045663
20-24	39.614732024823965
25-29	39.63212849697079
30-34	39.80220067089857
35-39	39.702302412221
40-44	39.41566920809554
45-49	39.70851907955138
50-54	39.64793452569937
55-59	39.549860400084526
60-64	39.645097251748055
65-69	39.979667768014565
70-74	40.13740905264075
75-79	40.20707564419821
80-84	40.43509126040754
85-89	40.57537499269593
90-94	40.4705961895504
95-99	40.94867845453284
100-101	40.67987138551604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.0
6	4.0
7	7.5
8	12.5
9	18.0
10	31.5
11	51.0
12	94.5
13	178.5
14	262.5
15	407.5
16	599.5
17	868.5
18	1261.5
19	1800.5
20	2669.5
21	3878.5
22	5384.0
23	7403.0
24	9835.5
25	12635.0
26	15965.0
27	19898.5
28	24651.5
29	29879.0
30	35487.5
31	41729.5
32	47953.5
33	55196.0
34	64223.0
35	74708.0
36	87480.0
37	99709.0
38	107707.5
39	108733.0
40	103169.5
41	94603.0
42	84280.5
43	74574.5
44	67713.5
45	62079.0
46	56573.5
47	51201.0
48	45540.5
49	38915.0
50	33110.5
51	29598.0
52	26349.5
53	23263.0
54	20363.5
55	17578.5
56	15678.5
57	13832.5
58	11682.5
59	9795.5
60	8196.0
61	6973.0
62	5813.0
63	4446.5
64	3401.0
65	2798.0
66	2365.0
67	1944.0
68	1555.5
69	1478.0
70	1457.5
71	1254.0
72	1084.0
73	905.0
74	681.0
75	514.5
76	365.0
77	265.5
78	184.0
79	125.0
80	102.5
81	71.5
82	44.0
83	26.5
84	18.0
85	15.0
86	10.5
87	5.0
88	2.5
89	1.5
90	2.0
91	2.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014775546023587145
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012760698838552534
20-24	6.044541555103831E-4
25-29	0.016622489276535538
30-34	0.006559446946834899
35-39	0.04042007325088877
40-44	0.04835633244083065
45-49	0.04905033536012035
50-54	0.04897198019181345
55-59	0.039367875276481806
60-64	0.051333828836492915
65-69	0.04495347941721665
70-74	0.003951339201762319
75-79	0.0014887481978311289
80-84	1.5671033661380306E-4
85-89	0.0034252402145588377
90-94	0.0027536244862139676
95-99	0.002238719094482901
100-101	5.5967977362072514E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1786736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.84524047182995
#Duplication Level	Relative count
1	100.0
2	7.902778808799149
3	2.989755771657635
4	1.9857471605671442
5	1.55333679756514
6	1.2217603246480093
7	0.9699849058471284
8	0.8023408309207434
9	0.6718135253507534
10++	5.625046395961696
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1953	0.10930545978812761	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	451010	2.4338334	5.2538	4
CTTTG	441950	2.3403478	10.393288	9
GAGAG	248235	2.0871117	5.323914	7
ACACA	397715	2.0795975	8.650107	6
GCTTT	369760	1.9580654	5.639155	1
TACAC	345195	1.7936568	10.941323	5
GCCAA	223935	1.7677058	5.481576	1
TGGCT	216960	1.7454207	5.527076	6
CACAT	327885	1.7037128	6.2529044	7
GACTC	207930	1.6310703	6.248823	7
TTGAG	294705	1.620782	6.082296	9
GAGTC	197140	1.5959808	8.961155	9
ATGGA	271730	1.503859	5.3666677	4
ATACA	419905	1.4915663	5.440594	6
CCATG	188355	1.4775175	5.822853	9
AAGAC	272420	1.4700891	6.3291545	5
GTCTT	275975	1.4614267	6.3376307	1
CATGA	270880	1.4526105	5.6186523	9
TGTGT	263690	1.4411138	5.228016	8
TATGA	386990	1.4097956	6.879693	4
GACTT	262195	1.3972179	7.870001	7
TGAGT	252840	1.3905381	6.1169605	8
TCATA	387335	1.3672433	7.062569	2
GTGTT	245645	1.3424946	6.1696715	1
ATGAG	242520	1.3421996	5.594751	7
ACTCA	257090	1.335857	5.8296614	8
GATTG	237815	1.3079054	6.7302566	7
AACAC	243225	1.2717903	5.3822327	5
GTGTA	229590	1.2626706	11.785161	1
GTTCT	234180	1.240101	5.3566837	1
TGGAC	148625	1.2032193	8.792373	5
TATAC	337095	1.1899025	6.5377398	5
ACCAT	228600	1.1878213	5.1696053	8
GTCCA	150815	1.1830418	8.570205	1
ACACC	154635	1.1827649	5.5812607	6
GTATG	213340	1.1733009	6.3542795	3
ATGTG	211450	1.1629065	5.215682	7
ACATG	216680	1.1619598	6.4116597	8
GGACT	138150	1.118417	8.819848	6
ACACT	212870	1.1060871	5.771718	6
TGTAT	291605	1.0556475	5.420953	2
AGACT	194680	1.0439833	6.1204567	6
GAGTA	181830	1.0063176	5.209182	1
CATAC	190030	0.9874089	6.192441	3
ATACT	225645	0.7964981	5.4441223	6
GTATA	218130	0.79464257	7.1493087	1
>>END_MODULE