##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-152_CTCTCTAC-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2104103	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.747302769873908	33.0	31.0	34.0	30.0	34.0
2	31.94781529231221	33.0	31.0	34.0	30.0	34.0
3	32.09347783829974	34.0	31.0	34.0	30.0	34.0
4	35.63650448671002	37.0	35.0	37.0	33.0	37.0
5	35.505252832204505	37.0	35.0	37.0	33.0	37.0
6	35.63993635292569	37.0	35.0	37.0	33.0	37.0
7	35.6581350817902	37.0	35.0	37.0	33.0	37.0
8	35.710003740311194	37.0	35.0	37.0	33.0	37.0
9	37.53112846661974	39.0	38.0	39.0	35.0	39.0
10-14	37.663533011454284	39.2	37.2	39.4	34.8	39.4
15-19	38.72569669830802	40.0	38.2	41.0	35.0	41.0
20-24	38.63100323510779	40.0	38.0	41.0	34.2	41.0
25-29	38.450600849863335	40.0	38.0	41.0	34.0	41.0
30-34	38.19643230393189	40.0	38.0	41.0	33.4	41.0
35-39	37.89012087336029	40.0	38.0	41.0	33.0	41.0
40-44	37.86053981197689	40.0	37.8	41.0	32.8	41.0
45-49	37.72622652027967	40.0	37.4	41.0	32.4	41.0
50-54	37.020687010094086	39.2	36.4	40.2	31.6	40.6
55-59	37.678018138845864	40.0	36.8	41.0	32.6	41.0
60-64	37.24106500489758	39.2	35.8	41.0	32.0	41.0
65-69	36.57311624003197	38.4	35.0	40.4	31.0	41.0
70-74	35.69135284727031	36.6	34.8	39.4	30.4	41.0
75-79	34.62801878044944	35.4	34.0	38.0	29.0	39.6
80-84	33.51688847931874	35.0	33.4	36.4	27.4	38.2
85-89	32.497311681034624	35.0	32.8	35.4	25.4	36.6
90-94	31.635100087780877	35.0	31.6	35.0	22.6	36.0
95-99	30.807540600436386	34.2	30.6	35.0	19.0	35.0
100-101	29.635080839673723	33.5	28.5	35.0	13.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	44.0
7	245.0
8	462.0
9	683.0
10	806.0
11	877.0
12	1189.0
13	1577.0
14	1952.0
15	2419.0
16	2845.0
17	3391.0
18	3907.0
19	4587.0
20	5378.0
21	6382.0
22	7456.0
23	8665.0
24	10425.0
25	12204.0
26	14694.0
27	17939.0
28	21725.0
29	27288.0
30	33936.0
31	43502.0
32	57265.0
33	77245.0
34	108970.0
35	165353.0
36	276424.0
37	486995.0
38	577972.0
39	119208.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.013888202159585	23.870573290075725	10.961372427459198	24.15416608030549
2	14.87557768437527	24.232388738262685	39.929097131795	20.96293644556705
3	15.398142161709897	28.37828972389138	33.181952156385485	23.041615958013235
4	11.139174914667656	18.807724122194017	44.60861214904399	25.444488814094335
5	10.820370608960813	39.360740666434346	38.74901766328183	11.069871061323008
6	24.914094736752055	44.146227630174394	19.779074951213417	11.160602681860135
7	23.188730905649994	34.41091663811118	27.24126475518407	15.159087701054755
8	20.839036668643825	38.34608979331113	24.8683480099162	15.946525528128848
9	22.76455723635527	15.317398344463237	25.577886127548666	36.340158291632825
10-14	20.166479732015656	26.207981410910307	33.15557193115139	20.46996692592264
15-19	19.525138512201075	31.8083284353089	29.014001536055957	19.652531516434067
20-24	19.498840448069156	30.28590111678831	30.55990961265841	19.655348822484118
25-29	19.49723891084907	30.68584988743197	30.15534245442504	19.661568747293924
30-34	19.383474851565786	30.52296808166522	30.200762961800486	19.89279410496851
35-39	19.494562291252212	30.690221325886856	30.159026649753912	19.65618973310702
40-44	19.222594679002643	30.135146324622873	30.703667624516797	19.938591371857687
45-49	19.14410607705732	29.640210585993877	31.080902521179993	20.134780815768817
50-54	18.536458221443432	29.321187215150097	31.545661441134964	20.59669312227151
55-59	18.44422480153717	29.295142083169125	31.573275022133807	20.687358093159897
60-64	18.283469007192842	28.68830984137414	31.58580040732578	21.44242074410724
65-69	18.836509610589225	28.56001919589068	30.703823340005123	21.899647853514974
70-74	19.278739981113294	28.246703026065767	30.070858902150487	22.403698090670453
75-79	19.97332818490684	28.39184202543813	29.449541380335347	22.18528840931968
80-84	20.667284064574677	28.04122813638549	29.208821248475914	22.08266655056392
85-89	21.29609649323768	27.868892197416294	29.09975438271618	21.735256926629845
90-94	21.944553183698403	27.25297013429652	29.280669396946145	21.521807285058937
95-99	22.8653987300481	26.57604924744841	28.87175200679949	21.686800015704
100-101	23.82566890923652	25.78440435649494	28.852106835362417	21.537819898906125
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.16805428246506
2	35.83851412994232
3	38.43975811972313
4	36.58366372876199
5	21.89024167028382
6	36.07469741861219
7	38.34781860670475
8	36.78556219677267
9	59.10471552798809
10-14	40.63644665793829
15-19	39.17767002863514
20-24	39.154189270553275
25-29	39.15880765814299
30-34	39.276268956534295
35-39	39.15075202435923
40-44	39.16118605086033
45-49	39.27888689282613
50-54	39.13315134371494
55-59	39.13158289469707
60-64	39.72588975130008
65-69	40.7361574641042
70-74	41.68243807178375
75-79	42.158616594226515
80-84	42.749950615138594
85-89	43.03135341986753
90-94	43.46636046875734
95-99	44.5521987457521
100-101	45.36348880814264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	3.5
2	5.5
3	8.0
4	15.0
5	18.5
6	28.5
7	44.0
8	56.0
9	67.0
10	81.5
11	96.0
12	137.5
13	215.0
14	285.5
15	375.5
16	557.5
17	787.0
18	1112.0
19	1605.5
20	2333.0
21	3468.0
22	5146.5
23	7341.0
24	9929.0
25	13126.5
26	17146.5
27	21660.5
28	26880.5
29	32618.0
30	38916.5
31	45726.5
32	52583.0
33	60238.5
34	69110.5
35	79532.5
36	90837.5
37	100861.0
38	107906.0
39	111190.5
40	110910.0
41	107875.0
42	103380.5
43	98778.5
44	94584.5
45	89589.0
46	83313.0
47	76108.0
48	67784.0
49	59083.0
50	51225.0
51	44528.5
52	38448.0
53	33025.5
54	28066.0
55	23349.5
56	19495.5
57	16104.0
58	12758.5
59	9986.5
60	7679.0
61	6002.0
62	4743.0
63	3399.5
64	2319.5
65	1770.0
66	1397.5
67	1005.0
68	797.5
69	806.0
70	777.0
71	624.5
72	515.5
73	428.5
74	324.0
75	261.5
76	200.0
77	144.5
78	99.0
79	60.5
80	50.0
81	45.0
82	32.0
83	21.0
84	18.0
85	18.5
86	21.0
87	12.0
88	10.0
89	16.0
90	13.5
91	8.0
92	6.5
93	7.5
94	6.5
95	6.0
96	6.5
97	4.0
98	1.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03493175001413904
2	0.03146233810797285
3	0.027707769058834097
4	0.026757245248925553
5	0.03326833334679909
6	0.001948573810312518
7	5.703142859451272E-4
8	0.003374359525175336
9	5.703142859451272E-4
10-14	0.007566169526872021
15-19	0.025369480486459078
20-24	0.02117767048476239
25-29	0.001996100000807945
30-34	0.00188203714361892
35-39	0.0061118680977119465
40-44	0.013991710481853787
45-49	0.008716303336861362
50-54	0.004762124287641813
55-59	0.0038876423825259508
60-64	0.011130633814029067
65-69	0.015179865244239468
70-74	0.01906750762676542
75-79	0.007566169526872021
80-84	0.010427246194696742
85-89	0.008526198574879653
90-94	0.009096512860824779
95-99	0.00859273524157325
100-101	0.005608090478460418
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2104103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.414577667680874
#Duplication Level	Relative count
1	100.0
2	7.484002596679959
3	2.773501499273548
4	2.0229373396395562
5	1.5567714612507342
6	1.2117839809576803
7	1.04547281214257
8	0.8445392438715261
9	0.7548919595659835
10++	5.957525734953167
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GACGC	579500	5.6835413	14.54592	75-79
GCTGC	592995	5.5805364	14.092371	75-79
GTCTC	885630	5.494222	9.664403	55-59
CGCTG	570895	5.3725586	14.163572	75-79
CTGTC	856085	5.3109326	9.549472	55-59
GCCGA	525010	5.1491218	14.786341	80-84
CTGCC	551790	4.970977	13.507689	80-84
CGACG	504370	4.9466915	14.508411	85-89
TGCCG	516540	4.8610363	13.905847	80-84
CCGAC	504745	4.7389336	14.03912	80-84
TCTCT	1095165	4.4788084	7.321777	60-64
CTCTT	1029220	4.2091184	7.090901	60-64
TGACG	618500	4.1772575	10.115682	75-79
TGTCT	975010	4.1653256	7.0621104	55-59
CTGAC	642755	4.15566	9.622014	70-74
ACACA	893505	4.1362042	7.689527	70-74
GAAGG	560560	4.1216526	11.719851	85-89
TACAC	851140	3.7806396	7.9549875	5
ACGCT	579015	3.7435565	9.666696	75-79
GACGA	530975	3.737372	10.740689	85-89
ATCTG	836260	3.7232475	7.673766	70-74
CACAT	832180	3.696422	6.9906044	65-69
ACATC	809310	3.594837	7.1193867	70-74
CATCT	830785	3.5408885	7.008643	70-74
CGAAG	499875	3.5184686	10.752296	85-89
TCTGA	774305	3.4474075	7.15982	70-74
AAGGC	467375	3.2897108	10.329687	85-89
GGCTT	498420	3.2300286	9.694152	90-94
AGGCT	471765	3.1862311	10.029633	90-94
ATACA	948020	3.0220976	5.266828	65-69
TCTTA	1024690	3.0074754	5.029163	60-64
TATAC	885285	2.7079048	5.3786316	5
ACGAA	547205	2.6461349	7.45975	85-89
AGAAG	502025	2.535971	5.4177775	5
AGGTG	328325	2.3163946	9.461555	95-97
GGTGT	327405	2.2164261	8.406656	95-97
TTAGG	448200	2.0845363	6.889147	95-97
CAAAG	420950	2.0356002	5.252059	4
GGTGG	195070	2.003224	10.609071	95-97
CTTAG	441960	1.967721	6.4592676	90-94
GCTTA	440200	1.9598851	6.4130597	90-94
CTTTG	419960	1.7941048	8.1835575	9
AGAGA	352285	1.7795618	5.271915	8
GTGTA	378340	1.7596238	8.898079	1
GGGGG	109880	1.7117037	5.2486205	95-97
CTCGG	179385	1.68815	9.799767	95-97
AGATC	360345	1.6720133	5.2121706	95-97
GATCT	369270	1.6440862	5.194872	95-97
GAGAG	223045	1.6399921	5.961911	7
TAGGT	350770	1.6313983	6.0657873	95-97
TGTAG	337235	1.5684484	5.3138614	95-97
GTAGA	301800	1.4628423	5.5054574	90-94
TCTCG	227185	1.4093977	6.5033097	95-97
CGGTG	142805	1.4038649	8.951217	95-97
AAGAC	266015	1.2863764	5.86429	5
GAGTC	181780	1.2277153	6.681698	9
GGTCG	119390	1.1736805	5.23055	95-97
TATGA	360490	1.1518621	5.621709	4
GTCTT	262995	1.1235371	6.1444974	1
GATTG	237735	1.1056831	5.304871	7
TCATA	360235	1.1018848	5.501836	2
CGCCG	80575	1.1011316	5.170021	95-97
TGAGT	235600	1.0957534	5.099708	8
GACTT	245390	1.0925403	5.9074035	7
GTGTT	244490	1.0910835	5.489944	1
TCGGT	162475	1.052925	5.8693705	95-97
TGGAC	145560	0.98309076	6.431102	5
AGACT	201105	0.9331342	5.2361794	6
GTCCA	143390	0.927072	7.1695614	1
GGACT	134820	0.91055447	6.029791	6
GTATA	215875	0.68977845	6.9579067	1
>>END_MODULE