##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-152_CTCTCTAC-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2104103	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40917578654657	34.0	31.0	34.0	31.0	34.0
2	32.596609101360535	34.0	31.0	34.0	31.0	34.0
3	32.708442980215324	34.0	31.0	34.0	31.0	34.0
4	36.23995070583522	37.0	35.0	37.0	35.0	37.0
5	35.95941596015024	37.0	35.0	37.0	35.0	37.0
6	36.04456625935137	37.0	36.0	37.0	35.0	37.0
7	36.04332915261278	37.0	36.0	37.0	35.0	37.0
8	36.06827374895621	37.0	36.0	37.0	35.0	37.0
9	37.93593421995026	39.0	38.0	39.0	35.0	39.0
10-14	38.118773748243306	39.2	38.2	39.4	35.2	39.4
15-19	39.23744873706278	40.0	39.0	41.0	36.0	41.0
20-24	39.17317859439391	40.0	39.0	41.0	36.0	41.0
25-29	38.97381687113226	40.0	38.6	41.0	35.6	41.0
30-34	38.772661794598456	40.0	38.0	41.0	35.0	41.0
35-39	38.54472209773	40.0	38.0	41.0	34.2	41.0
40-44	38.31168977944521	40.0	38.0	41.0	33.8	41.0
45-49	38.1474668302835	40.0	38.0	41.0	33.4	41.0
50-54	38.11648868900429	40.0	37.4	41.0	33.2	41.0
55-59	37.75916435649775	39.8	36.6	41.0	33.0	41.0
60-64	37.22094973487515	39.0	35.6	40.8	32.0	41.0
65-69	36.4912269028655	37.8	35.0	40.0	31.0	41.0
70-74	35.886478846330235	36.6	35.0	39.2	31.0	41.0
75-79	34.65088363069678	35.2	33.6	37.4	30.2	39.2
80-84	34.428300990968594	35.0	34.0	36.4	31.0	38.2
85-89	33.806051319731026	35.0	34.0	35.4	30.0	36.6
90-94	33.39980352672849	35.0	34.0	35.0	29.6	36.0
95-99	33.132247233144	35.0	34.0	35.0	29.0	35.2
100-101	32.75633607290138	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	8.0
14	27.0
15	65.0
16	152.0
17	379.0
18	713.0
19	1203.0
20	1653.0
21	2518.0
22	3244.0
23	4704.0
24	6658.0
25	8408.0
26	10773.0
27	13787.0
28	17925.0
29	22628.0
30	28772.0
31	36202.0
32	46790.0
33	62443.0
34	89263.0
35	141005.0
36	259815.0
37	518201.0
38	688266.0
39	138397.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.586273580713495	23.85976351918133	11.271976704562467	24.28198619554271
2	15.094750371225244	24.094324484134756	39.7252838138189	21.085641330821105
3	15.490116215793618	28.091162837560706	33.434009646866144	22.984711299779526
4	11.323067359345051	18.822795271904464	44.308905029839316	25.545232338911166
5	10.716015328146959	39.42920094691182	38.822053863332734	11.032729861608486
6	24.85795609815679	44.4702089203808	19.62665325794412	11.045181723518288
7	23.123867985550138	34.37830752581979	27.288920741997895	15.208903746632174
8	20.760865794117496	38.486946694149474	24.786999495747118	15.965188015985909
9	22.68344277822901	15.098785563254271	25.641425348473913	36.57634631004281
10-14	20.113340459093497	26.203241951558454	33.17835676295314	20.505060826394907
15-19	19.486373532281256	31.837225324341034	29.016593272808432	19.659807870569278
20-24	19.43415653115517	30.34730689868747	30.543544012814678	19.67499255734268
25-29	19.459238694876166	30.712844882545664	30.138117486851545	19.68979893572663
30-34	19.371622686534927	30.51032403007426	30.203408307323716	19.914644976067095
35-39	19.449612158368254	30.677202336825232	30.187270091672353	19.685915413134165
40-44	19.209057224757053	30.145110816893595	30.695101240348087	19.95073071800126
45-49	19.132157498124865	29.624329390583142	31.061613472015736	20.181899639276253
50-54	18.52591026258567	29.232052350708283	31.576013279779293	20.66602410692676
55-59	18.25033319995192	29.208456127912267	31.526715713496728	21.014494958639084
60-64	17.88490652112945	28.670879569368296	31.348880477961476	22.095333431540777
65-69	18.220100327333782	28.73006987235	30.226288507696285	22.823541292619932
70-74	18.114017564957006	28.432072885645844	29.588719781683743	23.86518976771341
75-79	18.070594341084316	28.457462128305128	29.120621954129078	24.35132157648148
80-84	17.559913524989696	28.109188007865786	29.01336590974418	25.317532557400334
85-89	17.405051409227948	27.869688322257968	29.089732479242535	25.635527789271546
90-94	17.404042505805702	26.992905211346073	29.51280562822752	26.090246654620703
95-99	17.490055166745925	26.402654224167076	29.67292622340056	26.434364385686436
100-101	17.55714905591599	25.4954961805577	30.430425696840885	26.516929066685424
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.8682597762562
2	36.18039170204635
3	38.474827515573146
4	36.868299698256216
5	21.748745189755443
6	35.90313782167508
7	38.332771732182316
8	36.7260538101034
9	59.259789088271816
10-14	40.618401285488396
15-19	39.14618140285054
20-24	39.10914908849785
25-29	39.1490376306028
30-34	39.28626766260203
35-39	39.135527571502415
40-44	39.159787942758314
45-49	39.31405713740112
50-54	39.191934369512424
55-59	39.264828158591
60-64	39.980239952670225
65-69	41.04364161995371
70-74	41.97920733267041
75-79	42.4219159175658
80-84	42.87744608239003
85-89	43.04057919849949
90-94	43.494289160426405
95-99	43.92441955243236
100-101	44.074078122601414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.5
6	3.0
7	6.0
8	9.5
9	12.0
10	27.0
11	50.5
12	85.5
13	148.0
14	208.5
15	300.0
16	473.5
17	701.5
18	1046.5
19	1551.5
20	2277.5
21	3442.0
22	5109.5
23	7188.0
24	9716.0
25	13011.0
26	16926.5
27	21455.0
28	26813.5
29	32381.0
30	38504.0
31	45178.5
32	51980.5
33	59719.0
34	68556.0
35	78316.5
36	89504.5
37	100147.0
38	107078.0
39	110207.5
40	110165.5
41	107694.5
42	104032.5
43	100040.5
44	96299.5
45	91817.5
46	86417.0
47	79374.5
48	70626.0
49	61163.5
50	52194.5
51	44853.5
52	38293.5
53	32367.0
54	26996.0
55	22463.0
56	18783.0
57	15268.0
58	12106.0
59	9534.0
60	7315.5
61	5758.5
62	4524.5
63	3162.5
64	2169.0
65	1656.0
66	1272.5
67	943.0
68	722.5
69	675.5
70	681.5
71	589.5
72	502.5
73	439.5
74	336.0
75	247.5
76	174.5
77	111.5
78	68.0
79	43.0
80	30.0
81	21.0
82	14.0
83	6.0
84	4.5
85	4.0
86	1.5
87	0.0
88	0.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012309283338315661
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010170604766021436
20-24	7.509138098277508E-4
25-29	0.017004870959263875
30-34	0.006549109050269878
35-39	0.04150937477870618
40-44	0.049816952877306864
45-49	0.050054583829784005
50-54	0.050187657163171195
55-59	0.04093906049276105
60-64	0.05092906573489986
65-69	0.0412337228738327
70-74	0.0034599066680671055
75-79	0.0011881547623856816
80-84	1.8059952388262363E-4
85-89	0.003060686667905516
90-94	0.002452351429564047
95-99	0.002167194286591483
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2104103.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.95485856399063
#Duplication Level	Relative count
1	100.0
2	7.57945451271831
3	2.90127710828413
4	2.0531828369560494
5	1.5360067549529077
6	1.2361315477410022
7	1.0753289003954876
8	0.8710660780917258
9	0.7673825178341932
10++	6.15209819516102
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	623920	6.1859303	14.984848	75-79
GAGCC	604600	5.99438	15.152137	75-79
CGAGC	596315	5.912237	14.973311	75-79
CTCCG	651145	5.268516	12.066894	70-74
GTCTC	884830	5.217813	9.153941	55-59
TCTCT	1412165	5.162688	10.665321	90-94
CTGTC	858905	5.0649343	9.0664015	55-59
AGCCC	577070	4.8668537	12.558548	75-79
GCCCA	566025	4.7737036	12.633945	80-84
CCACG	519730	4.3832636	12.596324	80-84
TGTCT	979310	4.2088833	7.1006317	55-59
ACATC	1052615	4.180982	11.049544	95-97
CGAGA	544835	4.1036315	11.577029	85-89
TCCGA	666855	4.098918	9.4032135	75-79
GAGAC	541840	4.0810738	11.7362585	85-89
CATCT	1058510	4.033615	10.764542	95-97
ACGAG	533015	4.0146046	11.298284	80-84
ATCTC	1041840	3.9700923	10.686325	95-97
CCCAC	548630	3.9358904	10.753148	80-84
TCTCC	776840	3.8967626	8.154678	70-74
CTCTT	1031235	3.7700582	6.3337245	60-64
ACACA	895330	3.706816	6.886357	70-74
TACAC	854745	3.3950434	7.343129	5
CACGA	529160	3.3902676	9.673475	80-84
CACAT	836300	3.3217797	6.28204	65-69
AGACC	498195	3.1918788	9.849699	85-89
GACCT	494750	3.0410504	9.574783	85-89
ATACA	948515	2.8620696	5.0076537	65-69
AGAAG	498250	2.8508728	5.897927	5
CTCTC	558330	2.800679	8.132406	90-94
GAAGA	462320	2.64529	5.2570896	6
CCTCT	519710	2.6069543	7.949503	90-94
TATAC	888745	2.5727878	5.252138	5
ACCTC	478825	2.5035617	7.9403143	85-89
CAAAG	422105	2.054446	5.040587	4
AGAGA	355150	2.0320876	6.011669	8
GAGAG	222950	1.9740912	6.9504375	7
TCTCG	329820	1.9449376	8.123297	90-94
CTCTA	474105	1.8066504	6.001337	90-94
CTTTG	417650	1.7949783	8.287201	9
TGGAG	210790	1.7906078	5.32131	5
CTACA	448835	1.7827705	6.146838	95-97
AAGAG	303140	1.7344984	5.4318643	7
GTATG	324725	1.7101253	7.5660534	95-97
CTCGT	288645	1.70213	7.977472	90-94
TCTAC	433390	1.6514995	5.8263288	95-97
AGGAG	177390	1.5706842	5.5423193	7
TTGGA	292570	1.540785	5.0965743	4
ATGGA	278910	1.5310364	5.102102	4
TTGAG	288440	1.519035	5.339147	9
TGCCG	151770	1.4436198	12.143484	95-97
GCCGT	149170	1.4188889	11.662295	95-97
GTCTT	313880	1.348995	5.7351	1
ATGCC	219440	1.3488189	8.224699	95-97
GAGTC	181355	1.3104597	6.957913	9
AAGAC	266015	1.2947336	6.0623913	5
ATGAG	231460	1.2705666	5.147348	7
TGAGT	237365	1.2500544	5.4387283	8
GATTG	235685	1.2412068	6.082184	7
TATGA	361490	1.2302105	5.9516153	4
GTGTT	241970	1.2225443	6.2374535	1
CCGTC	149370	1.2085761	9.052041	95-97
TCGTA	266700	1.1947551	6.0008106	95-97
TATGC	259490	1.1624559	6.1007276	95-97
CGTAT	246785	1.1055404	6.130413	95-97
GACTT	246145	1.1026734	6.005603	7
GTGTA	205945	1.0845847	10.000018	1
TCATA	364205	1.0543206	5.3579016	2
TGGAC	143795	1.0390536	7.490442	5
GAGTA	179690	0.9863824	5.203721	1
GGACT	133925	0.9677335	6.7617173	6
AGACT	199220	0.9302465	5.508239	6
CGTCT	157065	0.92620707	6.0139847	95-97
GTCCA	145490	0.8942747	6.7894936	1
GGATA	162600	0.89256924	5.2649407	1
GTATA	215390	0.7330079	7.13864	1
>>END_MODULE