##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-151_CTCTCTAC-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1766755	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724287747876758	33.0	31.0	34.0	30.0	34.0
2	31.92530147077552	33.0	31.0	34.0	30.0	34.0
3	32.070007443024075	34.0	31.0	34.0	30.0	34.0
4	35.596494703566705	37.0	35.0	37.0	33.0	37.0
5	35.46500505163421	37.0	35.0	37.0	33.0	37.0
6	35.61031608796919	37.0	35.0	37.0	33.0	37.0
7	35.624082003447	37.0	35.0	37.0	33.0	37.0
8	35.67971846690684	37.0	35.0	37.0	33.0	37.0
9	37.4894164725726	39.0	37.0	39.0	35.0	39.0
10-14	37.615279424708014	39.2	37.2	39.4	34.6	39.4
15-19	38.674333113533	40.0	38.0	41.0	34.4	41.0
20-24	38.56492609331798	40.0	38.0	41.0	34.0	41.0
25-29	38.38128478481737	40.0	38.0	41.0	34.0	41.0
30-34	38.118503018245306	40.0	38.0	41.0	33.2	41.0
35-39	37.80417148953873	40.0	37.8	41.0	32.6	41.0
40-44	37.7730976847384	40.0	37.8	41.0	32.8	41.0
45-49	37.61607546037793	40.0	37.0	41.0	32.2	41.0
50-54	36.90665938401194	39.2	36.4	40.2	31.2	40.6
55-59	37.52561481359894	40.0	36.6	41.0	32.0	41.0
60-64	37.05738713064347	39.0	35.4	41.0	31.4	41.0
65-69	36.36310093929266	37.8	35.0	40.2	31.0	41.0
70-74	35.50564135944146	36.4	34.6	39.2	30.0	40.8
75-79	34.53810833986603	35.4	34.0	37.6	29.0	39.4
80-84	33.63308823238084	35.0	33.8	36.2	28.6	38.0
85-89	32.889597595591916	35.0	33.0	35.2	26.8	36.6
90-94	32.324356235018435	35.0	32.8	35.0	25.6	36.0
95-99	31.827851060277176	35.0	32.0	35.0	24.6	35.0
100-101	30.912960767055985	34.0	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	34.0
7	185.0
8	363.0
9	505.0
10	588.0
11	761.0
12	1138.0
13	1511.0
14	1917.0
15	2300.0
16	2659.0
17	3042.0
18	3484.0
19	4051.0
20	4617.0
21	5345.0
22	6189.0
23	7228.0
24	8488.0
25	10179.0
26	11653.0
27	14165.0
28	17001.0
29	20978.0
30	26090.0
31	33357.0
32	44000.0
33	59512.0
34	85544.0
35	135259.0
36	237818.0
37	431948.0
38	497440.0
39	87376.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98032494306645	23.091353008390268	10.487210760016172	24.44111128852711
2	15.586150978522555	23.34984021579361	39.4798350293183	21.584173776365525
3	16.147977454613397	27.19804780278732	32.48924961004182	24.164725132557475
4	11.40778636663966	18.098153083299504	43.65361238924926	26.840448160811576
5	11.040793516160338	38.99040492289852	38.62978267541053	11.339018885530605
6	26.266798813845117	43.24614525133341	19.106002828976003	11.381053105845474
7	24.48784375343145	33.45710136035401	26.220690467107328	15.834364419107217
8	21.639049185060237	37.95595731011861	23.89088099530479	16.51411250951636
9	23.275967952364375	14.500055186232307	24.52034673934269	37.70363012206063
10-14	20.86490465403599	25.577051985369025	32.33068151032436	21.227361850270626
15-19	20.265840730850837	31.18595003283068	28.206233491955494	20.34197574436299
20-24	20.241879912400222	29.62084631927632	29.761471542187678	20.375802226135775
25-29	20.242151330261038	30.009055232161597	29.34252946742805	20.40626397014932
30-34	20.12153629759186	29.828285829915973	29.38290556201764	20.667272310474523
35-39	20.298826475509497	30.008366145075072	29.292455073008483	20.400352306406948
40-44	20.023646606526288	29.691300039161934	29.792165507678014	20.492887846633767
45-49	20.106659312413562	29.499193024690186	29.78798557769379	20.606162085202463
50-54	19.779416625987288	29.14511942530224	30.229640007861157	20.84582394084931
55-59	19.90561711308501	29.109295749838026	30.229002915191817	20.756084221885143
60-64	19.60709614406399	28.717890833447207	30.46450631662881	21.21050670585999
65-69	19.970384406061743	28.797944049646595	29.874999278234533	21.35667226605713
70-74	19.855229104719776	28.464015945359698	29.90795458688371	21.772800363036822
75-79	19.966935610594188	28.603221440160997	29.69875246063995	21.731090488604863
80-84	19.86262613454578	28.29149887408679	29.723293666922153	22.122581324445278
85-89	20.02883739949874	28.08884074604997	29.78209830573485	22.100223548716436
90-94	20.03116526363732	27.602417821259607	30.13063303819092	22.23578387691215
95-99	20.484826899047626	27.195403311254307	29.808930566592178	22.510839223105894
100-101	20.722278873876437	26.54812555666972	30.34269203566422	22.386903533789624
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.42143623159356
2	37.170324754888085
3	40.312702587170875
4	38.24823452745124
5	22.379812401690945
6	37.64785191969059
7	40.322208172538666
8	38.153161694576596
9	60.979598074425
10-14	42.092266504306615
15-19	40.60781647521383
20-24	40.617682138536
25-29	40.648415300410356
30-34	40.78880860806638
35-39	40.699178781916444
40-44	40.516534453160055
45-49	40.712821397616025
50-54	40.6252405668366
55-59	40.66170133497016
60-64	40.817602849923986
65-69	41.32705667211887
70-74	41.6280294677566
75-79	41.698026099199055
80-84	41.985207458991056
85-89	42.12906094821518
90-94	42.26694914054948
95-99	42.99566612215352
100-101	43.109182407666054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	4.0
3	8.0
4	8.5
5	12.0
6	15.5
7	15.5
8	21.5
9	29.5
10	38.0
11	49.5
12	66.0
13	88.5
14	121.5
15	165.0
16	228.5
17	331.0
18	472.5
19	636.0
20	911.5
21	1264.0
22	1754.5
23	2541.0
24	3717.0
25	5404.0
26	7769.5
27	10820.5
28	14809.5
29	19773.5
30	25672.0
31	32305.0
32	39166.0
33	47407.0
34	57204.5
35	67723.0
36	80038.0
37	91414.5
38	99342.0
39	102937.0
40	101794.5
41	98543.0
42	93484.5
43	87623.5
44	82259.5
45	76354.5
46	70160.5
47	63099.5
48	55445.0
49	48000.0
50	41281.5
51	36202.5
52	31675.5
53	27964.5
54	24506.0
55	20994.5
56	18206.0
57	15312.5
58	12369.5
59	10220.0
60	8406.5
61	6646.0
62	5227.0
63	3781.0
64	2565.5
65	1923.0
66	1520.5
67	1135.5
68	837.0
69	824.5
70	842.0
71	719.0
72	588.5
73	473.5
74	379.0
75	284.5
76	192.5
77	141.0
78	103.5
79	69.5
80	56.0
81	44.5
82	34.5
83	23.0
84	18.0
85	16.0
86	19.5
87	17.0
88	9.5
89	9.5
90	9.0
91	6.5
92	7.5
93	9.5
94	7.0
95	5.5
96	6.0
97	6.0
98	4.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03741322367843872
2	0.03526238782400503
3	0.03056450951037354
4	0.02909288497839259
5	0.036281204807684146
6	0.0021508358544336937
7	5.094084918395589E-4
8	0.003396056612263727
9	5.660094353772877E-4
10-14	0.008286378133923493
15-19	0.0237271155310159
20-24	0.020251817597799354
25-29	0.0023545992511695172
30-34	0.002264037741509151
35-39	0.006282704732687893
40-44	0.01378798984579073
45-49	0.008954269267668692
50-54	0.005331808881254051
55-59	0.004460154350773027
60-64	0.011071144555979748
65-69	0.014331358903752926
70-74	0.0182594643852713
75-79	0.00800337341623485
80-84	0.010697578328630738
85-89	0.00906747115474415
90-94	0.009780643043319532
95-99	0.00914671247569697
100-101	0.006254404260919029
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1766755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.937227762369698
#Duplication Level	Relative count
1	100.0
2	7.761321825886791
3	2.985648782969641
4	2.076055866129763
5	1.5356360635598216
6	1.2067927853025668
7	1.052298490423215
8	0.8301741628456732
9	0.7036005236674091
10++	5.940274615160296
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	487155	3.528747	6.343215	90-94
CTGTC	458225	3.3191903	5.924456	90-94
TCTCT	670120	3.2969227	5.0618963	90-94
GCTGC	306925	3.273277	7.787439	90-94
CTCTT	621370	3.0570776	4.81899	90-94
GACGC	271255	2.9906144	7.867972	95-97
CGCTG	271055	2.890733	7.547009	95-97
ACACA	524625	2.851685	7.3728576	6
GCCGA	255870	2.8209932	7.8409677	90-94
CTGCC	271160	2.7588756	7.3622384	90-94
CGACG	245700	2.7088678	8.033745	95-97
AGAAG	453525	2.708582	5.3598266	5
TGCCG	241655	2.5771894	7.5081344	90-94
CCGAC	243310	2.5591671	7.3263583	90-94
TACAC	483350	2.5414538	9.032016	5
CTGAC	323475	2.4222906	5.989288	95-97
ATCTG	451125	2.4050784	5.0380754	95-97
TGACG	302845	2.3771136	6.0051246	95-97
GACGA	277985	2.2557087	6.3182373	95-97
CAAAG	383590	2.1855657	5.279202	4
CCTTG	289145	2.0944455	5.065091	95-97
ACGCT	278355	2.0844164	5.333093	95-97
CTCCT	300010	2.0732183	5.007865	90-94
CTTTG	375480	1.9363639	8.165568	9
AGAGA	318420	1.901696	5.091799	8
TATAC	489890	1.8338587	5.814695	5
GAGAG	209745	1.78401	5.9603996	7
GGTGG	151465	1.774806	5.204093	95-97
GCTTT	320860	1.6546868	5.1685953	1
GTGTA	266095	1.4870074	10.233763	1
TTGAG	264930	1.480497	5.4038663	9
AAGAC	240940	1.3727945	5.986781	5
GAGTC	169335	1.3291569	6.25037	9
CTCGG	124370	1.3263745	5.139967	95-97
CCATG	173000	1.2954828	5.3891773	9
TATGA	316255	1.2409341	6.2320123	4
GTCTT	240155	1.2384882	6.2718625	1
GATTG	215985	1.2069798	5.9594126	7
GACTT	224405	1.1963682	6.233492	7
TCATA	316815	1.1859683	6.186914	2
GTGTT	218595	1.181638	5.803201	1
GTTCT	221280	1.1411492	5.2136264	1
TGGAC	138060	1.0836709	7.2842155	5
ACATG	195530	1.0776504	5.212076	8
GTCCA	136050	1.0187886	7.2326717	1
GGACT	125875	0.98802745	6.6270328	6
AGACT	179015	0.98662907	5.524535	6
GAGTA	169560	0.979562	5.490159	1
GTATG	168995	0.94438756	5.4135747	3
GTATA	188655	0.74025214	7.029632	1
>>END_MODULE