##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-151_CTCTCTAC-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1766755	
Filtered Sequences	0	
Sequence length	101	
%GC	41	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.430683937501236	34.0	31.0	34.0	31.0	34.0
2	32.61781797702568	34.0	31.0	34.0	31.0	34.0
3	32.7277698379232	34.0	31.0	34.0	31.0	34.0
4	36.24525075633011	37.0	35.0	37.0	35.0	37.0
5	35.967675767155036	37.0	35.0	37.0	35.0	37.0
6	36.063882088914426	37.0	36.0	37.0	35.0	37.0
7	36.05977738848907	37.0	36.0	37.0	35.0	37.0
8	36.081134622514156	37.0	36.0	37.0	35.0	37.0
9	37.949664780911895	39.0	38.0	39.0	35.0	39.0
10-14	38.12664370555057	39.2	38.2	39.4	35.2	39.4
15-19	39.25245005674245	40.0	39.0	41.0	36.0	41.0
20-24	39.18131172686649	40.0	39.0	41.0	36.0	41.0
25-29	38.988522007861874	40.0	38.8	41.0	35.8	41.0
30-34	38.78893417593271	40.0	38.0	41.0	35.0	41.0
35-39	38.5638187524586	40.0	38.0	41.0	34.4	41.0
40-44	38.328938760609134	40.0	38.0	41.0	33.8	41.0
45-49	38.15144963506542	40.0	38.0	41.0	33.4	41.0
50-54	38.095262104819284	40.0	37.4	41.0	33.2	41.0
55-59	37.699927154585666	39.6	36.4	41.0	33.0	41.0
60-64	37.13042476178078	38.8	35.4	40.6	31.8	41.0
65-69	36.374068730525735	37.6	35.0	40.0	31.0	41.0
70-74	35.78019963152786	36.4	35.0	39.0	31.0	40.8
75-79	34.59690189075452	35.2	33.6	37.4	30.4	39.2
80-84	34.41569940370906	35.0	34.0	36.4	31.0	38.0
85-89	33.83326041245108	35.0	34.0	35.4	30.2	36.6
90-94	33.45952370306013	35.0	34.0	35.0	30.0	36.0
95-99	33.21201456908287	35.0	34.0	35.0	29.2	35.2
100-101	32.867227204677505	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	2.0
14	10.0
15	55.0
16	134.0
17	310.0
18	514.0
19	840.0
20	1281.0
21	1887.0
22	2567.0
23	3655.0
24	5062.0
25	6791.0
26	8604.0
27	11084.0
28	14637.0
29	18380.0
30	23489.0
31	29928.0
32	38844.0
33	52548.0
34	76629.0
35	122768.0
36	230537.0
37	448279.0
38	564168.0
39	103696.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54316812461264	23.095675404909	10.809648196835441	24.551508273642924
2	15.78672970489888	23.222308831862385	39.27503768651499	21.715923776723745
3	16.327730783272155	26.949294044731726	32.686309080772375	24.03666609122374
4	11.548460312833415	18.019249980897182	43.50110796346975	26.931181742799655
5	10.885663264006611	39.09246047131606	38.72393172794191	11.297944536735427
6	26.180766433376444	43.47371310679749	18.995106848431163	11.350413611394902
7	24.33925473537644	33.5141544809552	26.22304733819913	15.923543445469235
8	21.578543714323718	38.085020277288024	23.75235955183373	16.58407645655453
9	23.141182563513336	14.363338436851741	24.5486216255225	37.946857374112426
10-14	20.82413237828675	25.5477641212279	32.34674870030083	21.28135480018452
15-19	20.169404358806325	31.22536906289207	28.249172454850807	20.356054123450797
20-24	20.159661116337304	29.629962948749732	29.79510307673673	20.415272858176237
25-29	20.18547683124688	30.038483442156522	29.344717216330384	20.431322510266213
30-34	20.100405118634608	29.8033841585425	29.412246843306768	20.68396387951612
35-39	20.221084308082986	30.030020201832603	29.31580461875567	20.433090871328744
40-44	19.95114641036653	29.674365274810505	29.796782385889	20.577705928933973
45-49	20.06186877495567	29.525555695488027	29.784942924163747	20.627632605392556
50-54	19.688904044940536	29.139432113919245	30.24976264558521	20.92190119555501
55-59	19.79142389863434	29.08294779221797	30.21174694913484	20.913881360012844
60-64	19.39719656388065	28.681167724253314	30.37266833828291	21.548967373583125
65-69	19.723556811604148	28.902772167047363	29.609050345041517	21.76462067630697
70-74	19.47269413209884	28.588834847976962	29.567695723213323	22.37077529671087
75-79	19.531602611060226	28.79247124604057	29.205007260303294	22.470918882595907
80-84	19.150497155513143	28.47062206275814	29.15975143073359	23.219129350995132
85-89	19.119761344015735	28.416755457765852	29.059549431774702	23.40393376644371
90-94	18.908145174464092	27.744265971829858	29.521470278553203	23.82611857515285
95-99	18.954087882002888	27.51867534446994	29.305517632051902	24.221719141475273
100-101	18.843596788234244	26.73433879306344	30.03564729441677	24.386417124285554
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.09467639825556
2	37.50265348162262
3	40.3643968744959
4	38.479642055633065
5	22.18360780074204
6	37.53118004477135
7	40.26279818084568
8	38.16262017087825
9	61.08803993762576
10-14	42.105487178471265
15-19	40.52545848225712
20-24	40.57493397451354
25-29	40.616799341513094
30-34	40.784368998150725
35-39	40.65417517941173
40-44	40.5288523393005
45-49	40.689501380348226
50-54	40.610805240495544
55-59	40.705305258647186
60-64	40.94616393746378
65-69	41.488177487911116
70-74	41.84346942880971
75-79	42.00252149365614
80-84	42.369626506508276
85-89	42.52369511045945
90-94	42.73426374961694
95-99	43.17580702347816
100-101	43.230013912519794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	3.0
9	5.0
10	8.0
11	12.5
12	29.0
13	47.5
14	79.5
15	121.0
16	177.5
17	276.0
18	406.0
19	579.0
20	806.5
21	1196.5
22	1765.0
23	2573.0
24	3784.5
25	5486.5
26	7719.0
27	10695.0
28	14810.0
29	19864.0
30	25543.5
31	31968.0
32	38956.5
33	46908.5
34	56385.5
35	66827.0
36	78713.5
37	90119.0
38	97823.5
39	101423.0
40	100592.0
41	96552.0
42	91404.5
43	86509.0
44	82256.0
45	77061.0
46	71225.5
47	64896.5
48	57732.0
49	50004.5
50	43103.5
51	37851.0
52	32910.0
53	28798.5
54	25135.0
55	21542.5
56	18622.5
57	15801.5
58	12910.5
59	10483.5
60	8338.5
61	6719.0
62	5403.0
63	3763.5
64	2530.5
65	1872.5
66	1418.5
67	1070.0
68	795.5
69	780.0
70	793.0
71	679.0
72	580.0
73	469.5
74	354.0
75	261.5
76	158.5
77	99.5
78	67.0
79	33.5
80	17.5
81	13.5
82	10.0
83	8.5
84	7.0
85	2.5
86	0.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011772996255847585
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001335782267490399
20-24	7.358122659904741E-4
25-29	0.015904865134101786
30-34	0.006339305676225622
35-39	0.03835279934116502
40-44	0.04671841879604133
45-49	0.04662785728638096
50-54	0.047001423513729976
55-59	0.03768490820741982
60-64	0.04976354955837114
65-69	0.04491850879154156
70-74	0.0038941449153957392
75-79	0.001381063022320582
80-84	2.264037741509151E-4
85-89	0.0034752979332165466
90-94	0.0026942049123958897
95-99	0.0022979983076317884
100-101	5.6600943537728776E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1766755.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.402536866000105
#Duplication Level	Relative count
1	100.0
2	7.816701523752614
3	3.061821531719948
4	2.0036599940244995
5	1.6046708495169804
6	1.2013245692660093
7	1.0581615376954487
8	0.8564883975699632
9	0.7718354745543272
10++	6.114928791952993
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TCTCT	793805	3.8087513	7.7670283	90-94
GTCTC	487700	3.4872313	6.236754	90-94
CCGAG	315450	3.4435008	8.227113	95-97
GAGCC	310240	3.3866277	8.066558	95-97
CTGTC	462775	3.3090084	5.924708	90-94
CTCCG	333250	3.2540262	7.77897	95-97
CGAGC	297625	3.2489204	8.031271	95-97
TGTCT	562265	2.9440567	5.00781	90-94
AGCCC	289425	2.8951383	7.1291747	90-94
TCTCC	440605	2.8869622	6.0279403	95-97
GCCCA	287240	2.8732817	7.3484144	90-94
AGAAG	453470	2.7857187	5.4208517	5
CATCT	563615	2.7703443	7.019712	95-97
ATCTC	560220	2.7536564	6.9069004	95-97
ACATC	545055	2.7445676	7.0268073	95-97
ACACA	528825	2.727897	7.2505617	6
TCCGA	348235	2.5508378	5.8130536	95-97
CCCAC	274705	2.5180454	6.7826476	90-94
CCACG	250200	2.5027683	7.0923123	90-94
TACAC	483880	2.436527	8.957539	5
GAGAC	294265	2.4097266	6.574091	95-97
CGAGA	289235	2.3685362	6.372911	95-97
ACGAG	274725	2.2497144	6.109524	95-97
CTCTC	340890	2.2336028	5.174962	90-94
CAAAG	384765	2.1659474	5.082056	4
CACGA	263010	1.973627	5.378656	90-94
CTTTG	376245	1.9700438	8.222872	9
AGAGA	318690	1.9577495	5.2004433	8
AGACC	257855	1.9349438	5.605768	95-97
GACCT	257280	1.8845881	5.398681	95-97
GAGAG	207380	1.8532431	6.2826223	7
TATAC	493085	1.8181617	6.008785	5
TGGAG	200720	1.7509501	5.100795	5
GCTTT	319660	1.673761	5.0524983	1
TTGAG	263055	1.5398217	5.545694	9
AAGAC	241685	1.360511	5.846275	5
GTCTT	258020	1.3510098	6.045225	1
GAGTC	168845	1.3496928	6.379461	9
GTATG	222360	1.301609	5.7727437	3
CCATG	174025	1.2747414	5.4303193	9
TATGA	316615	1.2740269	6.5182495	4
GATTG	216985	1.2701459	6.3432064	7
GTGTT	217915	1.2451699	5.8905077	1
AGAAC	218305	1.2288985	5.073868	5
GACTT	224895	1.2063333	6.2105384	7
TCATA	320505	1.1818042	6.206022	2
GTTCT	221100	1.1576942	5.1108937	1
GTGTA	194675	1.1395518	10.74614	1
TGGAC	136710	1.092816	7.5111766	5
ACATG	198090	1.0885102	5.528349	8
GAGTA	169615	1.0171161	5.277532	1
GTCCA	138835	1.0169729	7.0575876	1
GGACT	125460	1.0028871	6.6740174	6
GCCGT	93685	0.9982913	5.680025	95-97
AGACT	178590	0.98135716	5.3605003	6
TGTAT	246265	0.9673138	5.129193	2
TGCCG	87715	0.93467605	5.7523603	95-97
GGATA	152180	0.9125651	5.120515	1
GTATA	188665	0.7591689	7.0944533	1
>>END_MODULE