##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-150_CTCTCTAC-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3195394	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.652843749471895	33.0	31.0	34.0	30.0	34.0
2	31.85289763953991	33.0	31.0	34.0	30.0	34.0
3	31.99385427900284	34.0	31.0	34.0	30.0	34.0
4	35.53026387356301	37.0	35.0	37.0	33.0	37.0
5	35.40278663601421	37.0	35.0	37.0	33.0	37.0
6	35.54274871893732	37.0	35.0	37.0	33.0	37.0
7	35.554848635254366	37.0	35.0	37.0	33.0	37.0
8	35.60175302325785	37.0	35.0	37.0	33.0	37.0
9	37.4007793718083	39.0	37.0	39.0	35.0	39.0
10-14	37.520236878456934	39.2	37.2	39.4	34.0	39.4
15-19	38.57033849346904	40.0	38.0	41.0	34.0	41.0
20-24	38.46852613480529	40.0	38.0	41.0	34.0	41.0
25-29	38.289127099819304	40.0	38.0	41.0	33.8	41.0
30-34	38.02554558217234	40.0	38.0	41.0	33.0	41.0
35-39	37.70931227886138	40.0	37.6	41.0	32.2	41.0
40-44	37.672053774902245	40.0	37.6	41.0	32.0	41.0
45-49	37.51343884353541	40.0	37.0	41.0	31.6	41.0
50-54	36.813783276804045	39.0	36.4	40.2	31.0	40.6
55-59	37.44781926735796	40.0	36.4	41.0	32.0	41.0
60-64	36.993438367850715	39.0	35.4	41.0	31.0	41.0
65-69	36.31746582737527	38.0	35.0	40.2	30.6	41.0
70-74	35.48031823305671	36.6	34.6	39.2	29.6	41.0
75-79	34.52163545403165	35.4	34.0	37.6	29.0	39.4
80-84	33.59336044318792	35.0	33.8	36.4	28.0	38.0
85-89	32.803064097885894	35.0	33.0	35.4	26.2	36.6
90-94	32.19003528203408	35.0	32.4	35.0	25.2	36.0
95-99	31.638968152284196	35.0	31.8	35.0	23.8	35.2
100-101	30.683979502997126	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	3.0
6	79.0
7	302.0
8	673.0
9	961.0
10	1187.0
11	1532.0
12	2242.0
13	3151.0
14	3913.0
15	4698.0
16	5456.0
17	6408.0
18	7172.0
19	8184.0
20	9429.0
21	10753.0
22	12593.0
23	14354.0
24	16482.0
25	19505.0
26	23004.0
27	26865.0
28	32508.0
29	39516.0
30	49279.0
31	62189.0
32	81523.0
33	110046.0
34	155915.0
35	241820.0
36	417847.0
37	759213.0
38	892223.0
39	174248.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.309799153235346	23.600402720366056	10.808709078363924	24.28108904803467
2	14.908630320401986	24.175547242912522	39.96379836654373	20.95202407014176
3	15.273042136804685	28.38145634994672	33.06103832519631	23.284463188052285
4	11.011620605178027	18.86446634181646	44.75560792337325	25.36830512963226
5	10.593667773327136	39.32993039152614	39.060199511942386	11.016202323204338
6	24.769922690518907	44.27189102627296	19.862869010142585	11.09531727306555
7	23.39826060831766	34.242356910154385	27.287074736259026	15.072307745268937
8	20.846857115316666	38.59055981337572	24.738545430988104	15.824037640319508
9	23.065940868341713	15.019631480219243	25.054406708689864	36.86002094274918
10-14	20.232834777531476	26.056909047502742	33.25153083918142	20.45872533578436
15-19	19.652735019271457	31.779137493931202	28.794902431305623	19.773225055491718
20-24	19.545978102723048	30.181396786368552	30.522781507956136	19.749843602952268
25-29	19.5558937162327	30.777048921342963	29.905100113127762	19.761957249296564
30-34	19.348888900989873	30.54582938773369	30.04111184947992	20.064169861796515
35-39	19.59934414346971	30.837087128205305	29.843331160289573	19.72023756803541
40-44	19.2306801486583	30.479565117506286	30.4623005228831	19.827454210952315
45-49	19.3887646723019	30.140394649867797	30.542912727345566	19.927927950484737
50-54	19.053863153643018	29.755313040784582	30.995206685140385	20.195617120432015
55-59	19.127887649495108	29.78261817977425	31.107713591062897	19.981780579667745
60-64	18.81369741627937	29.267769925811884	31.34283765209541	20.575695005813337
65-69	19.268735486436313	29.33139577101609	30.680922447928953	20.718946294618647
70-74	19.1954648428413	29.041362350072404	30.648644763968345	21.114528043117947
75-79	19.51312534133712	29.361035693104093	30.19031001625899	20.9355289492998
80-84	19.710761569308747	29.161347766230826	30.02311761627962	21.104773048180803
85-89	20.001986787549818	29.037964167466534	29.948587646938712	21.011461398044936
90-94	20.180592401215293	28.492021776199607	30.415664111053587	20.91172171153152
95-99	20.779141924570364	28.176594719705307	29.9212317482701	21.123031607454227
100-101	21.134784002083116	27.31433493072143	30.61896284945994	20.931918217735515
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.59088820127002
2	35.86065439054375
3	38.55750532485697
4	36.379925734810286
5	21.609870096531473
6	35.86523996358446
7	38.4705683535866
8	36.670894755636176
9	59.9259618110909
10-14	40.69156011331584
15-19	39.425960074763175
20-24	39.29582170567531
25-29	39.31785096552927
30-34	39.413058762786385
35-39	39.31958171150512
40-44	39.05813435961061
45-49	39.31669262278664
50-54	39.24948027407503
55-59	39.10966822916285
60-64	39.389392422092705
65-69	39.98768178105496
70-74	40.30999288595925
75-79	40.44865429063692
80-84	40.815534617489554
85-89	41.013448185594754
90-94	41.09231411274681
95-99	41.9021735320246
100-101	42.06670221981863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	11.0
3	18.0
4	20.0
5	31.0
6	45.0
7	54.5
8	68.5
9	84.5
10	109.5
11	151.0
12	231.5
13	341.0
14	484.0
15	698.0
16	1029.0
17	1504.5
18	2201.0
19	3149.0
20	4420.5
21	6468.0
22	9290.0
23	12928.0
24	17424.0
25	22906.5
26	29366.0
27	36322.5
28	44385.5
29	53241.5
30	62906.5
31	73226.0
32	83818.0
33	95924.5
34	110589.0
35	128200.0
36	149653.0
37	171009.0
38	185898.5
39	190063.0
40	184412.5
41	173129.0
42	157900.5
43	143390.0
44	132110.5
45	120686.0
46	109191.0
47	97995.5
48	86092.0
49	73886.0
50	63212.0
51	55634.5
52	48872.5
53	42720.0
54	37002.5
55	31780.0
56	27492.5
57	23131.5
58	18930.0
59	15511.5
60	12632.0
61	10097.0
62	7901.0
63	5479.0
64	3628.0
65	2850.5
66	2189.5
67	1488.5
68	1116.5
69	1185.5
70	1214.5
71	975.5
72	793.5
73	660.0
74	505.0
75	365.0
76	227.0
77	156.0
78	121.5
79	80.5
80	53.0
81	48.5
82	42.0
83	27.0
84	19.0
85	19.0
86	21.0
87	20.5
88	15.5
89	14.0
90	13.5
91	13.0
92	11.5
93	9.0
94	9.0
95	8.5
96	9.5
97	7.0
98	4.0
99	3.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03498786065192587
2	0.03326663316010483
3	0.02947993267809854
4	0.02804036059403003
5	0.03439325479111496
6	0.00172122749182104
7	7.510810873400901E-4
8	0.0030356193946661975
9	5.007207248933934E-4
10-14	0.0068348378947948206
15-19	0.02419107002141207
20-24	0.02004760602291924
25-29	0.0021530991170415916
30-34	0.001946551818023067
35-39	0.005726993290968188
40-44	0.013018738847228229
45-49	0.008074121688905969
50-54	0.004763105895548405
55-59	0.003824254536373292
60-64	0.010239738824069896
65-69	0.01413284246011603
70-74	0.018313860512975864
75-79	0.007479515828095065
80-84	0.010684128467412781
85-89	0.008962900975591742
90-94	0.009682687017625995
95-99	0.009375995573628792
100-101	0.005570518064439002
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3195394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	34.78872937187493
#Duplication Level	Relative count
1	100.0
2	8.898307873554518
3	3.0591224109675594
4	2.0758330645851295
5	1.7447791547923839
6	1.4874390380350009
7	1.2741955653255583
8	1.0839876529396666
9	0.9477487675975227
10++	11.060359749636367
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	3305	0.10343012473579159	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	894115	3.814438	6.360658	90-94
GCTGC	565435	3.721699	9.196754	90-94
CTGTC	841815	3.591318	5.9712787	80-84
GACGC	524540	3.5527878	8.994789	95-97
CGCTG	517515	3.4062886	8.848803	90-94
TCTCT	1206200	3.3353024	4.9578743	80-84
GCCGA	489885	3.3180642	9.281693	95-97
CTGCC	514050	3.2665806	8.816745	90-94
CGACG	475750	3.2223258	9.361632	95-97
CTCTT	1129320	3.122719	4.6916018	70-74
TGCCG	469935	3.0931165	8.831407	95-97
ACACA	1012745	3.0514927	7.741217	6
CCGAC	466425	3.050015	8.860569	95-97
TACAC	944295	2.7649531	9.19673	5
AGAAG	848910	2.7441945	5.195032	5
CTGAC	606490	2.6625206	6.1919947	95-97
TGACG	577565	2.6262777	6.331249	95-97
CACAT	892590	2.6135578	5.4654336	7
GACGA	528810	2.4744098	6.7503843	95-97
ACGCT	534200	2.345164	5.8832073	85-89
CAAAG	750050	2.3408465	5.268361	4
ATACA	1074270	2.1730762	5.355983	6
CTTTG	738420	2.1148999	9.593412	9
TGCAG	448210	2.0380807	6.1776004	95-97
TATAC	948760	1.8650295	5.764201	5
GCTTT	617975	1.7699349	5.4628625	1
GCCAA	382450	1.727731	5.535478	1
GGTGG	244395	1.7258055	5.8990707	95-97
GCAGT	366600	1.6669873	5.7570853	95-97
GTGTG	364195	1.66691	5.885853	95-97
GAGAG	342290	1.6589626	5.516354	7
TTGGC	371195	1.6402493	5.0093336	5
TGGCT	370385	1.6366699	5.5264225	6
GTGTA	531710	1.6231693	10.471047	1
CAGTG	353065	1.6054416	5.7931085	95-97
GACTC	342545	1.5037892	5.760945	7
TTGAG	490515	1.4974121	5.504219	9
GAGTC	328190	1.4923309	8.622116	9
AAGAC	459305	1.4334549	6.099492	5
CTCGG	216845	1.4272758	6.0550313	95-97
CATGA	455455	1.3813258	5.149204	9
CCATG	309945	1.3606737	5.382	9
TATGA	645590	1.314488	6.3201222	4
GACTT	441950	1.3025417	7.282145	7
GTCTT	453905	1.3000238	6.4812436	1
TCATA	648555	1.2749	6.457421	2
TGAGT	416185	1.2705022	6.311129	8
ATGAG	403145	1.2664336	5.7356515	7
ACTCA	424395	1.2426544	5.2865934	8
AACAC	401860	1.2108408	5.1038756	5
GATTG	394455	1.2041663	6.1881647	7
GTGTT	401195	1.1901793	5.8598294	1
CGGTG	169180	1.1533949	5.365685	95-97
GTTCT	398435	1.141153	5.103025	1
TGGAC	248425	1.1296271	7.909766	5
ACATG	361640	1.096799	5.6611056	8
GTCCA	248255	1.0898515	8.315927	1
GGACT	231715	1.0536442	7.770712	6
ACACT	348810	1.0213369	5.1046276	6
AGACT	333185	1.0104995	5.8153033	6
GAGTA	306805	0.9637926	5.1521277	1
GTATG	307295	0.93808997	5.3930902	3
CATAC	307060	0.89909035	5.2822022	3
GTATA	360360	0.7337302	7.206177	1
>>END_MODULE