FastQCFastQC Report
Thu 31 Jul 2014
Nextera-150_CTCTCTAC-ACTGCATA_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename Nextera-150_CTCTCTAC-ACTGCATA_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 3195394
Filtered Sequences 0
Sequence length 101
%GC 40

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT 3424 0.10715423512718618 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CCGAG 604790 4.062657 9.363495 85-89
GAGCC 582020 3.9097002 9.353536 90-94
TCTCT 1457565 3.810166 7.7710447 90-94
CGAGC 562400 3.7779036 9.157632 85-89
CTCCG 625500 3.7158415 8.40715 95-97
GTCTC 897225 3.709359 6.1677084 80-84
CTGTC 845980 3.4974988 5.7964196 75-79
AGCCC 550560 3.360169 8.465642 90-94
GCCCA 547600 3.3421035 8.570996 90-94
TGTCT 1047775 3.0146239 4.823765 90-94
TCTCC 802155 3.0130522 5.969633 95-97
CCACG 479760 2.9280634 8.203217 95-97
AGAAG 846730 2.9076288 5.2013164 5
CCCAC 522035 2.894723 7.6253514 90-94
ACACA 1012090 2.8688972 7.0257854 6
ACATC 1030335 2.842809 7.424113 95-97
TCCGA 666395 2.830452 6.171331 85-89
CATCT 1050975 2.8225067 7.339623 95-97
ATCTC 1050260 2.8205864 7.158183 95-97
CGAGA 552350 2.652859 6.9515014 95-97
GAGAC 547570 2.6299012 7.0042877 95-97
TACAC 950135 2.6215281 8.568338 5
ACGAG 518680 2.4911466 6.843599 95-97
CACAT 895560 2.4709496 5.04736 7
CAAAG 751185 2.343645 5.2687383 4
CTCTC 611300 2.2961633 5.749654 90-94
CACGA 506000 2.2080107 6.117632 95-97
ATACA 1079795 2.1301234 5.2223296 6
CTTTG 738920 2.1259966 9.688256 9
AGACC 481770 2.1022794 6.0054936 85-89
CCTCT 546650 2.0533252 5.5165205 90-94
GACCT 481700 2.0459769 5.801018 85-89
TATAC 954360 1.8325213 5.7535186 5
ACCTC 474790 1.8322153 5.289895 90-94
AGAGA 531680 1.8257627 5.0364857 8
GAGAG 338065 1.787101 5.971907 7
GCTTT 616425 1.7735578 5.4314804 1
TGGCT 369275 1.6803373 5.5068007 6
GCCAA 384400 1.67739 5.357758 1
TTGAG 490055 1.5943573 6.0446973 9
ATGGA 464290 1.551875 5.4128017 4
GAGTC 327660 1.5317808 8.762807 9
GACTC 344190 1.4619159 5.6900125 7
AAGAC 459055 1.4322197 6.186948 5
GTCTT 487945 1.4038993 6.222554 1
CATGA 455555 1.3834362 5.159298 9
TATGA 646805 1.3669709 6.6666584 4
GTATG 417200 1.357329 5.7165923 3
TGAGT 414725 1.3492768 6.655161 8
ATGAG 401165 1.3408817 6.13883 7
CCATG 313735 1.3325608 5.255487 9
GACTT 446025 1.3184112 7.3493333 7
GTGTT 403485 1.2777373 6.2914224 1
GATTG 391910 1.2750498 6.6677804 7
TCATA 652375 1.2526627 6.23664 2
GTGTA 383960 1.249185 10.813184 1
ACTCA 426005 1.175395 5.1904993 8
TGGAC 248765 1.1629537 8.372946 5
GTTCT 399790 1.1502626 5.000366 1
ATGTG 348140 1.1326474 5.164493 7
AACAC 398615 1.1299247 5.076927 5
ACATG 362050 1.0994788 5.686418 8
GGACT 232320 1.086075 8.051084 6
GTCCA 249315 1.0589428 7.9646544 1
GCCGT 161740 1.0575389 6.532071 95-97
GAGTA 309155 1.033341 5.4686637 1
CCGTC 171765 1.0203861 5.3413634 95-97
AGACT 332980 1.0111988 5.8719416 6
TGCCG 151055 0.9876749 6.8068385 95-97
ACACT 347375 0.95844626 5.0259557 6
GGATA 269310 0.9001604 5.0618367 1
GTATA 362240 0.76556545 7.1923037 1
Produced by FastQC (version 0.10.1)