##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-150_CTCTCTAC-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3195394	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.46254890633205	34.0	31.0	34.0	31.0	34.0
2	32.644718616859144	34.0	31.0	34.0	31.0	34.0
3	32.754994219805134	34.0	31.0	34.0	31.0	34.0
4	36.26743180966103	37.0	37.0	37.0	35.0	37.0
5	36.0005473503424	37.0	35.0	37.0	35.0	37.0
6	36.08891297911932	37.0	36.0	37.0	35.0	37.0
7	36.087575741833405	37.0	36.0	37.0	35.0	37.0
8	36.109769875013846	37.0	36.0	37.0	35.0	37.0
9	37.977783334386935	39.0	38.0	39.0	35.0	39.0
10-14	38.16349533109219	39.4	38.2	39.4	35.2	39.4
15-19	39.29729410520268	40.4	39.0	41.0	36.0	41.0
20-24	39.231392873617466	40.0	39.0	41.0	36.0	41.0
25-29	39.044474452915665	40.0	39.0	41.0	36.0	41.0
30-34	38.84896285090352	40.0	38.0	41.0	35.0	41.0
35-39	38.625816847625046	40.0	38.0	41.0	34.8	41.0
40-44	38.393441372175076	40.0	38.0	41.0	34.0	41.0
45-49	38.21856634893851	40.0	38.0	41.0	33.6	41.0
50-54	38.162119913851	40.0	37.6	41.0	33.6	41.0
55-59	37.783668179886426	39.8	36.6	41.0	33.0	41.0
60-64	37.230840641247994	39.0	35.6	40.8	32.2	41.0
65-69	36.492116465137	37.6	35.0	40.0	31.0	41.0
70-74	35.896662133057774	36.6	35.0	39.2	31.0	40.8
75-79	34.696085866093505	35.2	34.0	37.4	30.4	39.2
80-84	34.49282579863391	35.0	34.0	36.4	31.0	38.2
85-89	33.887497691990404	35.0	34.0	35.6	30.6	36.8
90-94	33.48971475818006	35.0	34.0	35.0	30.0	36.0
95-99	33.235544098787194	35.0	34.0	35.0	29.2	35.4
100-101	32.88598729921881	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	3.0
13	11.0
14	31.0
15	89.0
16	254.0
17	523.0
18	913.0
19	1589.0
20	2379.0
21	3369.0
22	4749.0
23	6812.0
24	9165.0
25	11964.0
26	15472.0
27	19931.0
28	25926.0
29	32451.0
30	41219.0
31	52572.0
32	68527.0
33	91809.0
34	133248.0
35	212528.0
36	397506.0
37	790053.0
38	1054921.0
39	217194.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.846261838133266	23.629762088806576	11.129676027431985	24.394300045628174
2	15.12213489110458	24.066977319505025	39.724700406135604	21.086187383254792
3	15.411432831131311	28.075692700180323	33.325937270959386	23.186937197728984
4	11.120569169247986	18.83013487538626	44.5914024999734	25.457893455392355
5	10.472292305737572	39.450878358036604	39.1372394139815	10.93958992224433
6	24.699896163039675	44.57913484221351	19.711747596696995	11.009221398049817
7	23.28858350488234	34.217188866224326	27.38225708629358	15.111970542599753
8	20.80237992560542	38.642777698149274	24.678678122322317	15.876164253922989
9	22.955791993100068	14.845399346684635	24.99845089525736	37.200357764957936
10-14	20.173581098293354	26.00508732256492	33.32221316056799	20.499118418573733
15-19	19.594286207859156	31.772096755416296	28.82292301702846	19.810694019696086
20-24	19.513178556192255	30.201227224566722	30.531955457591675	19.753638761649345
25-29	19.531062589925536	30.777707832216795	29.900926035824483	19.79030354203319
30-34	19.325598157478733	30.506508695735178	30.060343885976	20.10754926081009
35-39	19.550915816282604	30.81738618628772	29.89701469407306	19.734683303356608
40-44	19.195267760238995	30.4419016426128	30.497908629960378	19.864921967187822
45-49	19.37088500062808	30.122606332316426	30.539706068923905	19.96680259813159
50-54	19.000555627464216	29.743227221825734	31.00310451288103	20.25311263782902
55-59	19.010037539258494	29.71619298709071	31.106804208970313	20.16696526468048
60-64	18.61569090211829	29.21028987006533	31.270068635704746	20.90395059211164
65-69	18.961919992786502	29.38050952845512	30.460123506110808	21.197446972647565
70-74	18.750191689159237	29.16582010474709	30.229408578385623	21.85457962770804
75-79	18.80282247729434	29.36228875155734	29.761350012158296	22.07353875899002
80-84	18.447176130804486	29.149548249026846	29.59524049154832	22.80803512862034
85-89	18.348605822073	28.997260966773574	29.566079802509194	23.088053408644228
90-94	18.210557440330405	28.34436470124173	30.064834975134197	23.380242883293665
95-99	18.296291173067637	28.06869915811328	29.82859635786429	23.806413310954795
100-101	18.23162854510495	27.135371810406987	30.78187277179138	23.851126872696682
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.24056188376144
2	36.208322274359375
3	38.59837002886029
4	36.57846262464034
5	21.411882227981902
6	35.70911756108949
7	38.400554047482096
8	36.67854417952841
9	60.15614975805801
10-14	40.67269951686709
15-19	39.40498022755524
20-24	39.2668173178416
25-29	39.321366131958726
30-34	39.43314741828882
35-39	39.285599119639215
40-44	39.06018972742682
45-49	39.33768759875967
50-54	39.253668265293236
55-59	39.177002803938976
60-64	39.51964149422993
65-69	40.15936696543407
70-74	40.604771316867286
75-79	40.87636123628437
80-84	41.255211259424826
85-89	41.43665923071723
90-94	41.590800323624066
95-99	42.10270448402243
100-101	42.08275541780163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	1.5
6	4.5
7	13.0
8	18.5
9	27.5
10	47.5
11	85.0
12	144.5
13	242.5
14	382.5
15	554.0
16	867.5
17	1369.5
18	2137.5
19	3122.5
20	4477.0
21	6560.0
22	9414.0
23	13147.5
24	17589.0
25	22860.5
26	29157.0
27	36093.5
28	44046.5
29	52944.0
30	62219.5
31	72222.5
32	82550.5
33	94150.0
34	108809.5
35	126585.5
36	147727.0
37	168581.5
38	183484.0
39	187287.5
40	180311.5
41	168462.5
42	154286.5
43	141247.0
44	131811.0
45	122548.5
46	112542.5
47	102229.0
48	90577.5
49	77919.0
50	66973.5
51	58965.0
52	51481.0
53	44655.0
54	38526.5
55	32950.0
56	28324.5
57	24012.5
58	19727.5
59	15893.5
60	12670.5
61	10156.0
62	7988.5
63	5466.5
64	3612.0
65	2715.5
66	1976.5
67	1396.0
68	1043.5
69	1056.0
70	1143.0
71	960.0
72	818.0
73	681.5
74	507.0
75	366.0
76	209.5
77	118.5
78	56.0
79	33.5
80	21.5
81	9.5
82	5.0
83	3.5
84	3.0
85	1.0
86	1.0
87	2.0
88	1.0
89	0.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01258060821294651
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011892117216218095
20-24	6.634549604837463E-4
25-29	0.016536301939604318
30-34	0.006478074378308277
35-39	0.039087511586990524
40-44	0.047155374266835325
45-49	0.04786890129980841
50-54	0.04780005220013557
55-59	0.03894981338764484
60-64	0.050209770688685026
65-69	0.04324975261266686
70-74	0.0036552612917217717
75-79	0.001408277038762669
80-84	1.6273423559035285E-4
85-89	0.0033172748024187316
90-94	0.0025536756969563065
95-99	0.0022219482167144334
100-101	6.259009061167418E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3195394.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.5299328365547
#Duplication Level	Relative count
1	100.0
2	9.296419098143236
3	3.089522546419098
4	2.136604774535809
5	1.7062334217506632
6	1.4801061007957559
7	1.3037135278514589
8	1.1120689655172413
9	1.0019893899204244
10++	11.499336870026525
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	3424	0.10715423512718618	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	604790	4.062657	9.363495	85-89
GAGCC	582020	3.9097002	9.353536	90-94
TCTCT	1457565	3.810166	7.7710447	90-94
CGAGC	562400	3.7779036	9.157632	85-89
CTCCG	625500	3.7158415	8.40715	95-97
GTCTC	897225	3.709359	6.1677084	80-84
CTGTC	845980	3.4974988	5.7964196	75-79
AGCCC	550560	3.360169	8.465642	90-94
GCCCA	547600	3.3421035	8.570996	90-94
TGTCT	1047775	3.0146239	4.823765	90-94
TCTCC	802155	3.0130522	5.969633	95-97
CCACG	479760	2.9280634	8.203217	95-97
AGAAG	846730	2.9076288	5.2013164	5
CCCAC	522035	2.894723	7.6253514	90-94
ACACA	1012090	2.8688972	7.0257854	6
ACATC	1030335	2.842809	7.424113	95-97
TCCGA	666395	2.830452	6.171331	85-89
CATCT	1050975	2.8225067	7.339623	95-97
ATCTC	1050260	2.8205864	7.158183	95-97
CGAGA	552350	2.652859	6.9515014	95-97
GAGAC	547570	2.6299012	7.0042877	95-97
TACAC	950135	2.6215281	8.568338	5
ACGAG	518680	2.4911466	6.843599	95-97
CACAT	895560	2.4709496	5.04736	7
CAAAG	751185	2.343645	5.2687383	4
CTCTC	611300	2.2961633	5.749654	90-94
CACGA	506000	2.2080107	6.117632	95-97
ATACA	1079795	2.1301234	5.2223296	6
CTTTG	738920	2.1259966	9.688256	9
AGACC	481770	2.1022794	6.0054936	85-89
CCTCT	546650	2.0533252	5.5165205	90-94
GACCT	481700	2.0459769	5.801018	85-89
TATAC	954360	1.8325213	5.7535186	5
ACCTC	474790	1.8322153	5.289895	90-94
AGAGA	531680	1.8257627	5.0364857	8
GAGAG	338065	1.787101	5.971907	7
GCTTT	616425	1.7735578	5.4314804	1
TGGCT	369275	1.6803373	5.5068007	6
GCCAA	384400	1.67739	5.357758	1
TTGAG	490055	1.5943573	6.0446973	9
ATGGA	464290	1.551875	5.4128017	4
GAGTC	327660	1.5317808	8.762807	9
GACTC	344190	1.4619159	5.6900125	7
AAGAC	459055	1.4322197	6.186948	5
GTCTT	487945	1.4038993	6.222554	1
CATGA	455555	1.3834362	5.159298	9
TATGA	646805	1.3669709	6.6666584	4
GTATG	417200	1.357329	5.7165923	3
TGAGT	414725	1.3492768	6.655161	8
ATGAG	401165	1.3408817	6.13883	7
CCATG	313735	1.3325608	5.255487	9
GACTT	446025	1.3184112	7.3493333	7
GTGTT	403485	1.2777373	6.2914224	1
GATTG	391910	1.2750498	6.6677804	7
TCATA	652375	1.2526627	6.23664	2
GTGTA	383960	1.249185	10.813184	1
ACTCA	426005	1.175395	5.1904993	8
TGGAC	248765	1.1629537	8.372946	5
GTTCT	399790	1.1502626	5.000366	1
ATGTG	348140	1.1326474	5.164493	7
AACAC	398615	1.1299247	5.076927	5
ACATG	362050	1.0994788	5.686418	8
GGACT	232320	1.086075	8.051084	6
GTCCA	249315	1.0589428	7.9646544	1
GCCGT	161740	1.0575389	6.532071	95-97
GAGTA	309155	1.033341	5.4686637	1
CCGTC	171765	1.0203861	5.3413634	95-97
AGACT	332980	1.0111988	5.8719416	6
TGCCG	151055	0.9876749	6.8068385	95-97
ACACT	347375	0.95844626	5.0259557	6
GGATA	269310	0.9001604	5.0618367	1
GTATA	362240	0.76556545	7.1923037	1
>>END_MODULE