##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-149_CTCTCTAC-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2298827	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.626996289846954	31.0	31.0	34.0	30.0	34.0
2	31.84037772307355	33.0	31.0	34.0	30.0	34.0
3	31.98849674203409	34.0	31.0	34.0	30.0	34.0
4	35.528045390105476	37.0	35.0	37.0	33.0	37.0
5	35.36811643503404	37.0	35.0	37.0	33.0	37.0
6	35.49328722866053	37.0	35.0	37.0	33.0	37.0
7	35.516854900347006	37.0	35.0	37.0	33.0	37.0
8	35.569880639125955	37.0	35.0	37.0	33.0	37.0
9	37.3957379132923	39.0	37.0	39.0	34.0	39.0
10-14	37.50866133032194	39.2	37.2	39.4	34.0	39.4
15-19	38.52739436242919	40.0	38.0	41.0	34.0	41.0
20-24	38.39588642381528	40.0	38.0	41.0	34.0	41.0
25-29	38.16830131192996	40.0	38.0	41.0	33.2	41.0
30-34	37.839788466030726	40.0	37.8	41.0	32.4	41.0
35-39	37.43337197622962	40.0	37.0	41.0	31.4	41.0
40-44	37.338638705739925	40.0	36.8	41.0	31.0	41.0
45-49	37.12633721458813	39.8	36.0	41.0	30.8	41.0
50-54	36.34810283679458	38.6	34.8	40.0	29.8	40.6
55-59	36.965047130558325	39.0	35.0	41.0	31.0	41.0
60-64	36.50659505913232	38.8	35.0	41.0	30.4	41.0
65-69	35.83517115468019	37.4	34.8	40.0	29.4	41.0
70-74	34.96558392606316	36.2	34.0	39.0	28.6	40.8
75-79	33.96458524282166	35.0	33.6	37.2	27.0	39.4
80-84	32.96873127033918	35.0	33.0	36.2	25.6	38.0
85-89	32.082962136776715	35.0	32.0	35.2	24.2	36.6
90-94	31.330956526959188	34.6	31.0	35.0	21.2	35.8
95-99	30.60423407242041	34.0	30.0	35.0	18.6	35.0
100-101	29.48046220963996	33.5	27.5	35.0	13.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	7.0
6	65.0
7	322.0
8	599.0
9	837.0
10	1015.0
11	1432.0
12	1948.0
13	2618.0
14	3117.0
15	3581.0
16	4397.0
17	5066.0
18	5890.0
19	6944.0
20	8078.0
21	9284.0
22	10858.0
23	12391.0
24	14524.0
25	17110.0
26	20350.0
27	24025.0
28	28816.0
29	35345.0
30	43350.0
31	55030.0
32	71386.0
33	94805.0
34	132690.0
35	197795.0
36	306639.0
37	498662.0
38	566912.0
39	112855.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.49313000019582	22.367979599962577	10.460241032012133	24.678649367829472
2	16.10245752548645	23.050419392850742	38.40333947758219	22.44378360408062
3	16.918570321477944	26.746794293017	31.811439290530235	24.52319609497482
4	12.172732154030513	17.896269853632894	42.43275371878962	27.498244273546966
5	12.389025478122756	37.50993233393529	37.45893257326864	12.642109614673311
6	26.929854745132975	41.529423996220615	19.124651392234252	12.416069866412156
7	24.748924115856617	32.64976137622689	25.85836914538715	16.742945362529348
8	22.218394059735864	36.46042006252955	23.94293775799314	17.378248119741443
9	23.235131885513038	14.766148775976461	24.306554863990616	37.692164474519885
10-14	21.54499698259091	24.98935902156595	31.673564016056655	21.79207997978649
15-19	20.91967206409735	30.34442668216829	27.745166548170914	20.99073470556344
20-24	20.87082116806106	28.908002347427647	29.19041418314113	21.030762301370167
25-29	20.883271974459397	29.343209915211215	28.73346670895013	21.04005140137926
30-34	20.73061129390401	29.16778682780979	28.823890831661576	21.27771104662463
35-39	20.87610778544187	29.313513655545353	28.75280181905726	21.05757673995552
40-44	20.516789871398135	28.789402571619604	29.398311458823144	21.295496098159116
45-49	20.420015085558646	28.52106796845519	29.691490841774694	21.36742610421147
50-54	19.900806491046154	28.303777772016065	30.085742420886113	21.709673316051674
55-59	19.89682621232289	28.301038741764234	30.12485103688421	21.677284009028664
60-64	19.694178634321695	27.714363004555963	30.19169391621036	22.39976444491198
65-69	20.122962862542444	27.61498390285835	29.4987223309848	22.76333090361441
70-74	20.038762745647784	27.153196294940756	29.251393278188704	23.556647681222753
75-79	20.03572027373072	27.182376559760197	28.98744500331631	23.79445816319277
80-84	20.023094340581398	26.815764467309656	29.028999659701732	24.13214153240721
85-89	20.25194565682134	26.62638081467435	28.987167357200892	24.134506171303418
90-94	20.614373052284567	25.969150484575245	29.277639026074414	24.138837437065778
95-99	21.301834355416663	25.40428637220005	28.843144831532225	24.450734440851058
100-101	21.852047938879625	24.6866784676577	29.012318825132894	24.448954768329784
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	67.17177936802528
2	38.546241129567065
3	41.441766416452765
4	39.67097642757748
5	25.031135092796063
6	39.34592461154513
7	41.491869478385965
8	39.59664217947731
9	60.927296360032926
10-14	43.337076962377395
15-19	41.910406769660796
20-24	41.90158346943123
25-29	41.923323375838656
30-34	42.00832234052864
35-39	41.93368452539738
40-44	41.81228596955726
45-49	41.787441189770114
50-54	41.610479807097825
55-59	41.57411022135156
60-64	42.093943079233675
65-69	42.88629376615686
70-74	43.595410426870544
75-79	43.83017843692349
80-84	44.155235872988605
85-89	44.386451828124756
90-94	44.753210489350344
95-99	45.75256879626772
100-101	46.30100270720941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	7.0
2	8.5
3	12.5
4	15.0
5	20.0
6	29.0
7	37.0
8	50.5
9	61.0
10	80.0
11	103.5
12	141.5
13	188.5
14	246.0
15	388.0
16	572.5
17	779.0
18	1131.5
19	1663.0
20	2439.0
21	3670.5
22	5393.0
23	7617.5
24	10375.5
25	13861.0
26	17914.5
27	22467.5
28	27848.5
29	33251.0
30	39270.5
31	45704.5
32	52101.0
33	59332.5
34	67528.5
35	77335.5
36	88961.5
37	100137.5
38	108288.0
39	111607.0
40	110089.0
41	105328.5
42	98862.5
43	93330.5
44	88774.5
45	83661.0
46	77681.0
47	71052.5
48	64066.5
49	56674.5
50	50319.0
51	45517.5
52	41373.5
53	37815.0
54	34425.0
55	31560.0
56	29454.5
57	27499.0
58	25531.5
59	23484.5
60	21419.5
61	19958.5
62	18621.5
63	16684.0
64	15017.0
65	13944.0
66	12894.0
67	11821.0
68	10732.0
69	9860.0
70	9016.5
71	7863.0
72	6829.0
73	5871.5
74	4845.5
75	3929.5
76	3068.5
77	2370.5
78	1846.0
79	1341.5
80	976.5
81	741.5
82	524.5
83	360.0
84	255.0
85	186.5
86	141.0
87	116.0
88	95.0
89	70.0
90	55.0
91	47.0
92	34.5
93	30.5
94	27.0
95	20.5
96	17.5
97	17.0
98	17.0
99	11.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03445235330888318
2	0.03232083145012652
3	0.02871029442406932
4	0.027535782379448297
5	0.03379984661742706
6	0.001740017843882989
7	8.700089219414945E-4
8	0.0032190330111835294
9	6.525066914561208E-4
10-14	0.007229774141333819
15-19	0.024925755613623816
20-24	0.02094111475113177
25-29	0.0024360249814361847
30-34	0.002201122572511981
35-39	0.006168363256565196
40-44	0.014015843732477476
45-49	0.008908891360680904
50-54	0.005576757189644979
55-59	0.0045066462156569415
60-64	0.011153514379289959
65-69	0.014981553635832534
70-74	0.018905293873788677
75-79	0.008195484044688879
80-84	0.011101313843973469
85-89	0.009578798230575855
90-94	0.01029220554656788
95-99	0.009622298676672929
100-101	0.006525066914561209
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2298827.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.504949367492245
#Duplication Level	Relative count
1	100.0
2	6.573985240965673
3	2.541498470394439
4	1.7154511280463889
5	1.3473803619161402
6	1.045683012629051
7	0.8839732334111713
8	0.7463992421362586
9	0.6480459062686675
10++	5.176523119067876
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	657435	4.7369924	10.61352	90-94
GACGC	600220	4.5541315	10.69582	85-89
GTCTC	884495	4.496643	7.4131417	80-84
CGCTG	614055	4.424428	10.382183	90-94
CTGTC	869900	4.4224443	7.192576	55-59
GCCGA	555945	4.2181973	10.823422	95-97
CGACG	534520	4.055637	10.808702	95-97
TGCCG	550030	3.9631116	10.197876	95-97
CTGCC	584620	3.885049	9.530383	90-94
TCTCT	1079080	3.8707032	5.988625	60-64
TGTCT	979010	3.8075924	6.0266075	80-84
ACACA	894635	3.7472935	6.5859094	70-74
CTCTT	1021355	3.6636412	5.754466	60-64
CCGAC	515235	3.6055658	9.83764	95-97
TGACG	619685	3.5969658	8.221879	85-89
CTGAC	639510	3.4236202	7.6038456	95-97
TACAC	844870	3.3605976	7.515155	5
ATCTG	817615	3.3485548	6.5785427	95-97
CACAT	823720	3.2764704	5.975305	90-94
GACGA	535120	3.2708583	8.5281315	85-89
ACATC	801240	3.1870525	5.9618006	90-94
CATCT	822550	3.1070175	5.795266	80-84
ACGCT	575765	3.082361	7.371388	75-79
TCTGA	749320	3.0688517	6.0649323	85-89
ATACA	932830	2.9891286	5.036208	90-94
GACTC	528745	2.8306391	7.6993713	85-89
TATAC	866375	2.6363482	5.2606378	5
ACGAC	465095	2.6219542	7.6635404	95-97
AGAAG	526460	2.5923364	5.0565076	5
CGACT	466745	2.4987218	7.0258646	85-89
ACTCC	487985	2.4094486	6.4282765	90-94
CTCCT	510830	2.3952034	6.390567	90-94
TCCTT	584515	2.0966787	5.147401	90-94
GGTGG	222360	1.8834786	7.5746217	95-97
CGCCG	199950	1.88322	5.204811	95-97
GTGTA	397660	1.7658244	9.3763895	1
CTTTG	452670	1.7605364	7.702789	9
ACGTG	297865	1.7289594	7.086358	95-97
CGTGT	278820	1.5368944	6.4647384	95-97
GAGAG	229695	1.522261	5.190442	7
CTCGG	211260	1.5221843	7.074675	95-97
CGGTG	184470	1.4411286	6.73347	95-97
TTACG	350815	1.4367679	5.2065496	95-97
AAGAC	285825	1.298073	5.64173	5
TACGT	307570	1.2596576	5.009889	95-97
TATGA	380290	1.2546968	5.9198685	4
GAGTC	201975	1.1723653	6.2969503	9
TCATA	378820	1.152736	5.85546	2
GTGTT	263080	1.1093746	5.635382	1
GATTG	248985	1.1056272	5.4309382	7
GTCTT	284115	1.1049877	5.6389804	1
GACTT	267885	1.0971271	5.7954617	7
TGGAC	169195	0.9820936	6.636341	5
GTCCA	170295	0.9116752	6.8089843	1
GAATA	261665	0.90910655	5.1098685	1
GGACT	151590	0.8799052	6.099708	6
TATAA	354755	0.8696427	5.1772923	2
GTATA	218770	0.7217914	7.0626755	1
>>END_MODULE