##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-149_CTCTCTAC-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2298827	
Filtered Sequences	0	
Sequence length	101	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3807180792639	34.0	31.0	34.0	31.0	34.0
2	32.568344203369804	34.0	31.0	34.0	31.0	34.0
3	32.68592373414789	34.0	31.0	34.0	31.0	34.0
4	36.22372583930848	37.0	35.0	37.0	35.0	37.0
5	35.916810181888415	37.0	35.0	37.0	35.0	37.0
6	36.00002871029442	37.0	35.0	37.0	35.0	37.0
7	36.00157993620225	37.0	35.0	37.0	35.0	37.0
8	36.02668230362703	37.0	35.0	37.0	35.0	37.0
9	37.907156997895015	39.0	38.0	39.0	35.0	39.0
10-14	38.08655266359757	39.2	38.2	39.4	35.2	39.4
15-19	39.18736077138471	40.0	39.0	41.0	36.0	41.0
20-24	39.09878585904899	40.0	39.0	41.0	36.0	41.0
25-29	38.86315290363303	40.0	38.0	41.0	35.0	41.0
30-34	38.60356346954338	40.0	38.0	41.0	34.4	41.0
35-39	38.29290686075986	40.0	38.0	41.0	33.8	41.0
40-44	37.96079278693003	40.0	37.4	41.0	33.0	41.0
45-49	37.714117156271435	40.0	37.0	41.0	32.8	41.0
50-54	37.64517712729145	40.0	36.2	41.0	33.0	41.0
55-59	37.24235447034509	39.0	35.2	41.0	32.0	41.0
60-64	36.67810705198782	38.4	35.0	40.2	31.0	41.0
65-69	35.95866953015603	37.2	34.8	39.8	30.6	41.0
70-74	35.41650293823763	36.0	34.0	39.0	30.2	40.8
75-79	34.26164143713294	34.8	33.4	37.0	29.4	39.2
80-84	34.13322829425615	35.0	34.0	36.2	30.0	38.0
85-89	33.56406776151489	35.0	34.0	35.2	29.4	36.6
90-94	33.180634906410965	35.0	33.8	35.0	29.0	36.0
95-99	32.917934755420916	35.0	33.0	35.0	29.0	35.0
100-101	32.52692655863186	35.0	32.5	35.0	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	4.0
13	20.0
14	55.0
15	174.0
16	374.0
17	646.0
18	1118.0
19	1745.0
20	2619.0
21	3540.0
22	4793.0
23	6513.0
24	8492.0
25	11209.0
26	14004.0
27	18070.0
28	22412.0
29	28269.0
30	35168.0
31	44659.0
32	58036.0
33	78010.0
34	114385.0
35	186136.0
36	314086.0
37	537302.0
38	671783.0
39	135103.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98810958806383	22.38672157582976	10.764446389397724	24.860722446708692
2	16.33365309944726	22.973215781219068	38.16797944795752	22.525151671376147
3	17.031077153696213	26.542232190591115	32.02933496083003	24.39735569488265
4	12.362087273204986	17.889601957868077	42.20239278553802	27.545917983388918
5	12.18417044866795	37.741683040959586	37.46449819842903	12.609648311943438
6	26.902720387397572	41.63714798895263	19.062025981076435	12.398105642573364
7	24.73748568291568	32.69680580574354	25.8450505409933	16.720657970347485
8	22.161389265046914	36.553946860725056	23.90023259688528	17.38443127734275
9	23.183736749220362	14.656996807502262	24.3430671381535	37.816199305123874
10-14	21.46283300135243	24.960703872018208	31.742466918998254	21.83399620763111
15-19	20.908945220481915	30.334363899647155	27.746893075728874	21.009797804142057
20-24	20.797203846086255	28.928332982717876	29.241746660061523	21.032716511134346
25-29	20.86205325766672	29.34081970789321	28.730221201971744	21.066905832468326
30-34	20.716711831804364	29.164271809384502	28.833968568683638	21.2850477901275
35-39	20.831800041847107	29.303941948801004	28.792446353365754	21.07181165598614
40-44	20.49066264360217	28.826454248768524	29.371733613917243	21.311149493712062
45-49	20.42222295339848	28.46645006440444	29.704157381163405	21.407169601033672
50-54	19.841264936359305	28.295456474290205	30.09802560425475	21.76525298509574
55-59	19.67646555037516	28.199851133280973	30.07752662611476	22.046156690229108
60-64	19.35074586980482	27.745526321882686	29.944060852711623	22.95966695560087
65-69	19.635535541468755	27.817799278472787	29.053894856936154	23.492770323122304
70-74	19.426408950856008	27.579288260153785	28.66777208441429	24.32653070457592
75-79	19.267706649389204	27.608843347726676	28.383849518351923	24.739600484532197
80-84	18.75297434740099	27.326750952095676	28.38825910783237	25.532015592670966
85-89	18.646068143530638	27.09959631170593	28.491089389390023	25.763246155373405
90-94	18.54512406030149	26.317257078266053	28.88638842037731	26.25123044105515
95-99	18.51781637266347	25.830412500023925	28.990750370675975	26.661020756636628
100-101	18.494497411070952	25.069089583513676	29.682203140993213	26.754209864422158
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.84883203477253
2	38.858804770823404
3	41.42843284857886
4	39.9080052565939
5	24.79381876061139
6	39.300826029970935
7	41.458143653263164
8	39.54582054238966
9	60.99993605434424
10-14	43.29682920898354
15-19	41.918743024623964
20-24	41.8299203572206
25-29	41.928959090135045
30-34	42.00175962193186
35-39	41.90361169783325
40-44	41.80181213731423
45-49	41.829392554432154
50-54	41.606517921455044
55-59	41.72262224060427
60-64	42.31041282540569
65-69	43.128305864591056
70-74	43.75293965543193
75-79	44.0073071339214
80-84	44.28498994007196
85-89	44.40931429890404
90-94	44.79635450135664
95-99	45.1788371293001
100-101	45.24870727549311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	1.0
6	3.5
7	7.0
8	10.0
9	17.5
10	26.0
11	45.0
12	87.0
13	140.0
14	194.0
15	294.0
16	446.0
17	672.5
18	1053.0
19	1586.0
20	2347.5
21	3604.5
22	5340.5
23	7584.5
24	10324.0
25	13740.5
26	17921.5
27	22309.0
28	27490.5
29	33223.5
30	38982.5
31	45111.5
32	51774.0
33	58806.0
34	66840.5
35	76657.5
36	88207.5
37	99521.0
38	108098.5
39	111582.5
40	109504.0
41	104893.5
42	99123.0
43	93505.0
44	89498.5
45	84992.0
46	79760.5
47	73845.5
48	66075.5
49	58100.0
50	51222.5
51	45950.5
52	41329.5
53	37490.0
54	34274.5
55	31249.0
56	29240.0
57	27344.0
58	25170.5
59	23028.0
60	21209.0
61	19793.5
62	18262.0
63	16514.0
64	14945.0
65	13836.5
66	12927.5
67	11914.5
68	10854.5
69	10022.0
70	9156.5
71	8064.0
72	6913.0
73	5802.0
74	4797.0
75	3802.5
76	2986.0
77	2287.0
78	1621.5
79	1157.0
80	794.5
81	536.5
82	349.0
83	224.0
84	149.5
85	88.0
86	53.5
87	37.0
88	27.5
89	18.0
90	10.0
91	8.0
92	7.0
93	5.5
94	4.0
95	2.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011832121338404326
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011397116877433578
20-24	5.307054423843116E-4
25-29	0.01712177558380861
30-34	0.006585967539097113
35-39	0.04081211852827551
40-44	0.04969490962129817
45-49	0.050434417204948435
50-54	0.05054751836480083
55-59	0.04056851603013189
60-64	0.05165242969566653
65-69	0.04257823663981675
70-74	0.0038367393457619907
75-79	0.001409414453545221
80-84	1.4790151673005407E-4
85-89	0.00318423265430587
90-94	0.002514325784410919
95-99	0.0023403240000226203
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2298827.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.82635100734599
#Duplication Level	Relative count
1	100.0
2	6.662635302654653
3	2.465985366148636
4	1.7252222289411623
5	1.3363971699824635
6	1.0951200338634577
7	0.9058474934994255
8	0.7855112777408236
9	0.61377517082905
10++	5.3504263167442705
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	631380	4.7939653	10.736185	85-89
TCTCT	1375150	4.7575426	10.038269	90-94
CGAGC	616455	4.680642	10.697894	85-89
GAGCC	604845	4.5924892	10.792655	90-94
GTCTC	889605	4.464617	7.405394	80-84
CTGTC	874405	4.388333	7.098489	55-59
CTCCG	648805	4.1305094	9.000459	95-97
TGTCT	983095	3.889376	6.1696167	80-84
ACATC	1027535	3.8668265	10.183135	95-97
AGCCC	579385	3.8469756	9.432129	90-94
GCCCA	573525	3.8080666	9.49974	90-94
CATCT	1037065	3.7419784	9.8886	95-97
ATCTC	1019625	3.6790512	9.741636	95-97
CTCTT	1024335	3.5438442	5.5393634	60-64
ACACA	897820	3.5237908	6.1893063	70-74
CCACG	524065	3.4796643	9.344086	95-97
TCCGA	652595	3.415808	7.486877	85-89
TCTCC	766575	3.3642616	6.7067595	95-97
CGAGA	538520	3.3617556	8.846315	85-89
GAGAC	526095	3.2841916	8.992994	95-97
ACGAG	525895	3.2829428	8.867881	95-97
TACAC	850005	3.1987445	7.0411735	5
CCCAC	550560	3.196723	8.195584	95-97
CACAT	829320	3.1209023	5.7259603	95-97
CACGA	524365	2.862504	7.748577	95-97
AGAAG	527270	2.706186	5.181046	5
AGACC	485630	2.6510499	7.7099524	85-89
GACCT	495425	2.59315	7.418349	85-89
TATAC	870725	2.5830727	5.264399	5
CTCTC	543210	2.3839815	6.559113	90-94
CCTCT	511870	2.2464397	6.379504	90-94
ACCTC	476040	2.1789198	6.4004736	90-94
CAAAG	460465	2.0666611	5.048621	4
CTTTG	452780	1.7913141	7.8934426	9
AGAGA	348190	1.7870669	5.098925	8
TCTCG	341010	1.7114099	6.4522533	90-94
CTACA	442095	1.6636947	5.4054184	95-97
CTCTA	453365	1.6358495	5.06341	90-94
GAGAG	228750	1.6329666	5.347574	7
GTATG	335170	1.581482	6.223842	95-97
TGCCG	211120	1.5369887	9.043942	95-97
CTCGT	300305	1.5071259	6.340311	90-94
TCTAC	416050	1.501208	5.088349	95-97
TTGAG	310400	1.4646058	5.261857	9
GCCGT	196570	1.4310623	8.432071	95-97
GTCTT	341165	1.3497365	5.726399	1
ATGCC	252205	1.3200896	6.6892514	95-97
TATGA	381470	1.2941	6.1582236	4
AAGAC	286850	1.2874416	5.821148	5
ATGAG	252450	1.2423307	5.1275554	7
GAGTC	201570	1.2065004	6.094429	9
CCGTC	189320	1.2052745	6.7778697	95-97
GTGTT	262440	1.1873162	5.903408	1
GATTG	251135	1.1849673	5.95505	7
TGAGT	250905	1.1838819	5.152045	8
TCATA	381580	1.1319864	5.5842376	2
TCGTA	269580	1.112333	5.0931454	95-97
GACTT	267660	1.104411	5.755715	7
TATGC	262870	1.0846466	5.2418656	95-97
GTGTA	224990	1.0616033	9.929824	1
CGTAT	247255	1.0202165	5.1371617	95-97
TGGAC	169900	1.016939	6.9244323	5
GGACT	151850	0.90890056	6.3265977	6
GTCCA	170155	0.8906241	6.40861	1
GTATA	217545	0.7380004	6.8908153	1
>>END_MODULE