##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-147_CTCTCTAC-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3435235	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.761575554510827	33.0	31.0	34.0	30.0	34.0
2	31.958692200096937	33.0	31.0	34.0	30.0	34.0
3	32.103348970303344	34.0	31.0	34.0	30.0	34.0
4	35.634159235103276	37.0	35.0	37.0	33.0	37.0
5	35.5120400787719	37.0	35.0	37.0	33.0	37.0
6	35.644026682308485	37.0	35.0	37.0	33.0	37.0
7	35.658128192103305	37.0	35.0	37.0	33.0	37.0
8	35.71106867506881	37.0	35.0	37.0	35.0	37.0
9	37.52995006164061	39.0	38.0	39.0	35.0	39.0
10-14	37.66582926641118	39.2	37.2	39.4	34.8	39.4
15-19	38.734614778901594	40.0	38.2	41.0	35.0	41.0
20-24	38.6390751724409	40.0	38.2	41.0	34.2	41.0
25-29	38.461778364507815	40.0	38.0	41.0	34.0	41.0
30-34	38.207315714936534	40.0	38.0	41.0	33.4	41.0
35-39	37.90257563165257	40.0	38.0	41.0	33.0	41.0
40-44	37.87428219612341	40.0	37.8	41.0	32.8	41.0
45-49	37.73251017761521	40.0	37.4	41.0	32.4	41.0
50-54	37.031822102418026	39.2	36.4	40.2	31.6	40.6
55-59	37.66413639823768	40.0	36.8	41.0	32.6	41.0
60-64	37.22404155756447	39.2	35.8	41.0	31.8	41.0
65-69	36.55986650112729	38.2	35.0	40.4	31.0	41.0
70-74	35.70808902447722	36.6	35.0	39.4	30.4	41.0
75-79	34.705407461207166	35.4	34.0	38.0	29.2	39.6
80-84	33.685908125644964	35.0	33.8	36.4	28.4	38.2
85-89	32.743176347469685	35.0	33.0	35.4	26.0	36.8
90-94	31.95923475395424	35.0	32.4	35.0	24.2	36.0
95-99	31.23315319039309	35.0	31.4	35.0	20.2	35.2
100-101	30.1666594861778	34.0	30.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	10.0
6	90.0
7	340.0
8	758.0
9	1031.0
10	1129.0
11	1455.0
12	2104.0
13	2924.0
14	3618.0
15	4167.0
16	4972.0
17	5740.0
18	6766.0
19	7813.0
20	9128.0
21	10613.0
22	12100.0
23	14249.0
24	16550.0
25	19240.0
26	22951.0
27	27869.0
28	34068.0
29	41808.0
30	52275.0
31	66906.0
32	88352.0
33	120113.0
34	170154.0
35	257429.0
36	437033.0
37	795553.0
38	981284.0
39	214472.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99370652341038	23.782364287810946	10.995733295438132	24.22819589334054
2	14.818307005847501	24.112078883742168	40.123763983580055	20.945850126830273
3	15.421275510308078	28.407928487421852	33.18559603569805	22.985199966572022
4	11.034507458931616	18.62452944903607	44.834946096290565	25.50601699574175
5	10.683498729354374	39.46086303730168	39.02144416512016	10.834194068223784
6	24.938882067279504	44.190521436834715	19.824741889902135	11.045854605983648
7	23.097916028061093	34.317550060127175	27.439647952575953	15.144885959235788
8	20.828075151894122	38.47856394079629	24.812910629401046	15.880450277908533
9	22.937818865854958	15.057949675450377	25.42185046555943	36.58238099313523
10-14	20.18401538470765	26.1556216348936	33.21185233610502	20.44851064429373
15-19	19.572233457839726	31.732754284761246	29.01500499910407	19.680007258294964
20-24	19.49623475510957	30.32133346835367	30.51143147320684	19.671000303329915
25-29	19.467370276907676	30.678320196727988	30.14525614153451	19.70905338482983
30-34	19.36284640035091	30.546173992087834	30.213887226507346	19.877092381053906
35-39	19.466328271082112	30.743669711470023	30.165026071074134	19.62497594637373
40-44	19.215526365543422	30.308157331200963	30.67358790943536	19.80272839382026
45-49	19.193771220132277	30.003106890542895	30.856127677829893	19.946994211494935
50-54	18.765235865713322	29.73352791457755	31.262551057926345	20.238685161782776
55-59	18.79871731177874	29.686737922077455	31.266209507489478	20.248335258654322
60-64	18.60371913795001	29.20698294730358	31.365287075363867	20.824010839382545
65-69	19.034406202391043	29.16975568081639	30.63740132914369	21.15843678764888
70-74	19.29422862757943	29.04348509662627	30.2382064999818	21.424079775812498
75-79	19.86680643689897	29.45446872503254	29.60512502755482	21.073599810513674
80-84	20.29621617681113	29.462030846118243	29.27043697551505	20.971316001555575
85-89	20.59759566427942	29.46680420988449	29.129156842878924	20.80644328295717
90-94	21.072639479936893	29.046085741730003	29.22659722119923	20.654677557133873
95-99	21.75402118820471	28.61370100700742	28.797238953923372	20.8350388508645
100-101	22.43083825236085	27.929571820809944	28.927986543409688	20.711603383419522
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.22190241675092
2	35.764157132677774
3	38.4064754768801
4	36.54052445467336
5	21.517692797578157
6	35.98473667326315
7	38.24280198729688
8	36.708525429802656
9	59.52019985899019
10-14	40.632526029001376
15-19	39.25224071613469
20-24	39.16723505843949
25-29	39.1764236617375
30-34	39.239938781404824
35-39	39.09130421745585
40-44	39.01825475936368
45-49	39.140765431627216
50-54	39.003921027496105
55-59	39.04705257043307
60-64	39.427729977332554
65-69	40.19284299003992
70-74	40.71830840339193
75-79	40.94040624741264
80-84	41.2675321783667
85-89	41.404038947236586
90-94	41.72731703707077
95-99	42.58906003906921
100-101	43.14244163578037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	9.0
2	13.0
3	18.0
4	23.5
5	34.0
6	51.5
7	65.5
8	73.5
9	105.5
10	144.5
11	190.0
12	270.5
13	390.0
14	553.5
15	786.5
16	1098.5
17	1574.0
18	2325.5
19	3322.0
20	4728.0
21	6905.5
22	9926.5
23	13843.5
24	18872.0
25	25001.5
26	32307.0
27	40333.5
28	49217.5
29	59173.0
30	70047.0
31	81658.5
32	93681.0
33	106128.0
34	120170.5
35	136189.5
36	154622.5
37	171518.0
38	182351.5
39	186510.5
40	183913.5
41	177329.0
42	168422.5
43	159075.0
44	149876.5
45	139119.0
46	127166.0
47	114209.0
48	100681.0
49	86515.5
50	73549.5
51	63828.5
52	54601.0
53	46606.0
54	39623.0
55	32910.0
56	27855.0
57	23145.0
58	18714.0
59	15121.0
60	12050.0
61	9698.0
62	7829.5
63	5714.0
64	4176.0
65	3446.0
66	2686.5
67	2003.0
68	1654.5
69	1695.5
70	1589.0
71	1274.0
72	1062.5
73	902.0
74	750.0
75	558.5
76	390.0
77	297.0
78	217.5
79	147.5
80	109.0
81	90.0
82	69.5
83	49.0
84	46.0
85	34.5
86	21.0
87	26.0
88	24.0
89	16.5
90	14.0
91	15.5
92	14.5
93	11.5
94	10.5
95	11.0
96	10.5
97	11.5
98	7.5
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03501943826259339
2	0.032545080613116714
3	0.02870254873392941
4	0.028033016664071014
5	0.03359304385289507
6	0.0021250365695505546
7	4.657614399014915E-4
8	0.00326033007931044
9	3.784311699199618E-4
10-14	0.006974777562524835
15-19	0.0238586297589539
20-24	0.02010925016774689
25-29	0.002200722803534547
30-34	0.002026062263571488
35-39	0.0058802381787563295
40-44	0.012738575381305791
45-49	0.008424460044218226
50-54	0.004832274938977974
55-59	0.003982260311157752
60-64	0.010275861767826655
65-69	0.014036885395031198
70-74	0.017757154896244364
75-79	0.0076908857763733785
80-84	0.010631004865751543
85-89	0.008453570134212069
90-94	0.00957139758997565
95-99	0.008878577448122182
100-101	0.00551636205383329
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3435235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.520623490269358
#Duplication Level	Relative count
1	100.0
2	8.979607236507007
3	2.685078634445723
4	1.6638683997057104
5	1.302923402944154
6	1.1023285354059373
7	0.9187119168443094
8	0.8212441912379659
9	0.6998811522571639
10++	7.591163764643739
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	1150950	4.6003194	7.5795255	90-94
GCTGC	743405	4.5339994	11.015376	90-94
CTGTC	1096125	4.3811855	7.1539855	55-59
GACGC	698150	4.3470716	10.78313	85-89
CGCTG	691995	4.2204514	10.610729	90-94
CTGCC	674820	3.9997957	10.497612	90-94
GCCGA	637835	3.9715166	11.105869	95-97
TCTCT	1513005	3.9632142	5.9464397	60-64
CGACG	621725	3.8712068	11.196472	95-97
TGCCG	623560	3.8030694	10.517259	90-94
CCGAC	612950	3.7090864	10.59548	95-97
CTCTT	1409050	3.690911	5.6372266	60-64
GAAGA	1202775	3.549605	6.911401	85-89
TGTCT	1298360	3.4995196	5.4764457	90-94
ACACA	1192700	3.3244097	6.5502453	6
CTGAC	793660	3.238606	7.376571	95-97
TGACG	761570	3.197714	7.542811	95-97
GAGGA	718570	3.1695454	8.386717	90-94
TACAC	1105310	3.0176952	7.790146	5
CACAT	1090720	2.9778616	5.456261	95-97
ATCTG	1080280	2.9726357	5.67906	95-97
CATCT	1101360	2.945293	5.372539	80-84
AGAGG	664535	2.9312024	8.237825	90-94
GACGA	679625	2.9133399	7.897442	95-97
ACGCT	707690	2.8877974	7.065768	75-79
ACATC	1051150	2.8698285	5.3160477	90-94
TCTGA	1017255	2.7992082	5.3186965	95-97
CGAAG	632470	2.7112012	7.6972647	85-89
AAGAG	910555	2.6872113	5.949895	90-94
TATAC	1159305	2.1343749	5.630131	5
ACGAA	712625	2.043857	5.302136	85-89
AGGAT	679220	1.9634234	5.3622355	90-94
CTTTG	690280	1.8605381	7.8008814	9
AGAGA	616060	1.8181036	5.143748	8
GGTGG	275215	1.7772166	6.5618124	95-97
GAGAG	392645	1.7319206	5.9509196	7
GGATA	591080	1.7086368	5.0905113	95-97
GCTTT	578765	1.5599675	5.044419	1
CTCGG	243330	1.4840606	6.4902906	95-97
GTGTA	522890	1.4805458	9.046255	1
AAGAC	441160	1.2652768	5.609695	5
GAGTC	295985	1.2427949	6.2411094	9
CCATG	299680	1.2228732	5.0600634	9
CGGTG	192615	1.2087938	5.656624	95-97
GTCTT	439060	1.1834153	6.2996397	1
GTGTT	409535	1.1358225	5.665527	1
TATGA	593645	1.1246204	5.3990374	4
GACTT	405610	1.116128	5.602048	7
GATTG	393490	1.1141537	5.189044	7
GTTCT	409885	1.1047788	5.100642	1
TCATA	595245	1.0958945	5.39609	2
TGGAC	238655	1.0020752	6.4527407	5
GTCCA	235710	0.96183735	6.88476	1
AGACT	332005	0.93270016	5.066574	6
GGACT	217660	0.9139204	5.835751	6
GTATA	364440	0.69040704	6.8897305	1
>>END_MODULE