##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-147_CTCTCTAC-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3435235	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45454590442866	34.0	31.0	34.0	31.0	34.0
2	32.63755143389026	34.0	31.0	34.0	31.0	34.0
3	32.74965118834665	34.0	31.0	34.0	31.0	34.0
4	36.26538912185047	37.0	37.0	37.0	35.0	37.0
5	35.99582037327868	37.0	35.0	37.0	35.0	37.0
6	36.07973748520843	37.0	36.0	37.0	35.0	37.0
7	36.0770474217921	37.0	36.0	37.0	35.0	37.0
8	36.101109822181016	37.0	36.0	37.0	35.0	37.0
9	37.97377471992455	39.0	38.0	39.0	35.0	39.0
10-14	38.161510289689055	39.4	38.2	39.4	35.2	39.4
15-19	39.29318989821657	40.4	39.0	41.0	36.0	41.0
20-24	39.22749762388891	40.0	39.0	41.0	36.0	41.0
25-29	39.03366628483932	40.0	39.0	41.0	36.0	41.0
30-34	38.836501374723994	40.0	38.0	41.0	35.0	41.0
35-39	38.611258269084935	40.0	38.0	41.0	34.6	41.0
40-44	38.37512787334783	40.0	38.0	41.0	34.0	41.0
45-49	38.20782461752981	40.0	38.0	41.0	33.4	41.0
50-54	38.16629272815397	40.0	37.6	41.0	33.6	41.0
55-59	37.800467158724224	39.8	36.8	41.0	33.0	41.0
60-64	37.25094655824128	39.0	35.6	40.8	32.2	41.0
65-69	36.51614099180987	37.8	35.0	40.0	31.0	41.0
70-74	35.927015997449956	36.6	35.0	39.2	31.0	41.0
75-79	34.716844466244666	35.2	34.0	37.4	30.4	39.2
80-84	34.50587444527085	35.0	34.0	36.6	31.0	38.4
85-89	33.88276103381573	35.0	34.0	35.6	30.4	36.8
90-94	33.47579108852815	35.0	34.0	35.0	30.0	36.0
95-99	33.21094894526866	35.0	34.0	35.0	29.2	35.4
100-101	32.84957957752526	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	5.0
13	13.0
14	41.0
15	122.0
16	289.0
17	589.0
18	1108.0
19	1767.0
20	2690.0
21	4011.0
22	5351.0
23	7453.0
24	10184.0
25	13310.0
26	17160.0
27	21812.0
28	28307.0
29	35506.0
30	44439.0
31	56920.0
32	73708.0
33	98958.0
34	141851.0
35	224926.0
36	417945.0
37	842499.0
38	1138766.0
39	245294.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.602375092242596	23.759306131894906	11.327318218404272	24.31100055745822
2	15.010156456300743	24.01203460735696	39.89782695215184	21.079981984190457
3	15.518210544547898	28.082620257420526	33.502715243644175	22.8964539543874
4	11.221532151366647	18.620822156271696	44.58670221978991	25.570943472571745
5	10.576394336923093	39.51371594665285	39.14008794158187	10.769801774842188
6	24.906156347382346	44.472386896384094	19.65143578241372	10.97002097381984
7	23.044333211556122	34.336864872417756	27.44176162620607	15.177040289820058
8	20.833945858143622	38.51410456635427	24.737230495148076	15.914719080354036
9	22.84533663635821	14.941394111319894	25.37913708960231	36.83413216271958
10-14	20.121977099092202	26.111034616263517	33.27342088678067	20.49356739786361
15-19	19.509583561316525	31.815907499627095	28.969355682536545	19.705153256519832
20-24	19.47494490006558	30.351161942573107	30.512264043729694	19.66162911363162
25-29	19.444225593745827	30.700049909605443	30.135263707133625	19.720460789515105
30-34	19.362647130216597	30.52967023536002	30.219946926368063	19.887735708055317
35-39	19.435415375794282	30.733450826746605	30.187798415038785	19.643335382420325
40-44	19.18449561539683	30.318472791996886	30.679814748171474	19.81721684443481
45-49	19.18630451161413	29.974710690980533	30.875890621845315	19.96309417556002
50-54	18.740172983451934	29.71786501512207	31.24197295269518	20.29998904873082
55-59	18.675386901393708	29.57709956361743	31.261792204873874	20.485721330114988
60-64	18.33967698077333	29.19123446563654	31.18717080314421	21.28191775044592
65-69	18.634620309755253	29.23059422420727	30.33469789913404	21.80008756690344
70-74	18.487604264285864	29.00708936592782	29.993215951201424	22.512090418584894
75-79	18.466069661566436	29.04092791108687	29.621163444992522	22.87183898235417
80-84	18.055924769153687	28.77271187611004	29.533907082531783	23.637456272204485
85-89	17.96153094195896	28.548696254947547	29.551236242606844	23.93853656048665
90-94	17.889624066016314	27.844738120682322	29.941385400525334	24.324252412776033
95-99	17.92172054870384	27.430912626428437	29.893379781568548	24.753987043299176
100-101	17.896854218124815	26.636591092021362	30.558142310496955	24.90841237935687
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.91337564970082
2	36.0901384404912
3	38.414664498935295
4	36.792475623938394
5	21.346196111765277
6	35.87617732120219
7	38.22137350137618
8	36.748664938497654
9	59.679468799077796
10-14	40.61554449695581
15-19	39.214736817836354
20-24	39.1365740136972
25-29	39.164686383260936
30-34	39.25038283827191
35-39	39.07875075821461
40-44	39.00171245983164
45-49	39.14939868717415
50-54	39.04016203218275
55-59	39.1611082315087
60-64	39.621594731219254
65-69	40.43470787665869
70-74	40.999694682870754
75-79	41.337908643920606
80-84	41.69338104135817
85-89	41.90006750244561
90-94	42.21387647879234
95-99	42.675707592003015
100-101	42.80526659748168
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	6.0
7	13.5
8	21.5
9	31.0
10	55.0
11	99.5
12	155.0
13	264.0
14	449.0
15	673.5
16	1012.5
17	1517.5
18	2240.0
19	3208.5
20	4678.0
21	6900.0
22	9841.5
23	13855.0
24	18868.5
25	24950.0
26	32159.5
27	40099.5
28	49246.0
29	58953.5
30	69178.0
31	80314.5
32	91819.0
33	104293.5
34	118355.0
35	133783.0
36	151450.5
37	168481.5
38	179633.5
39	183737.5
40	180767.5
41	173929.0
42	165519.5
43	156592.0
44	149288.5
45	140862.0
46	130943.0
47	119804.5
48	106151.5
49	91517.0
50	77794.5
51	67051.5
52	57603.0
53	48568.5
54	40671.0
55	33783.5
56	28462.0
57	23686.5
58	19042.5
59	15215.5
60	12136.0
61	9832.5
62	7875.5
63	5621.0
64	4010.5
65	3229.5
66	2578.5
67	1992.5
68	1573.0
69	1538.0
70	1549.0
71	1261.5
72	1079.0
73	946.0
74	716.5
75	550.0
76	385.5
77	264.0
78	164.0
79	106.5
80	76.0
81	47.5
82	33.5
83	26.5
84	17.5
85	7.5
86	6.0
87	3.5
88	3.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013798182657081685
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011702256177524974
20-24	6.462439978633193E-4
25-29	0.018211272300148316
30-34	0.006800117022561776
35-39	0.04397370194469956
40-44	0.0527300170148476
45-49	0.053777980254625955
50-54	0.053649895858653054
55-59	0.04380486342273527
60-64	0.0546978590984314
65-69	0.044177472574656464
70-74	0.0034408126372722685
75-79	0.001275021941730333
80-84	1.6883852196429065E-4
85-89	0.0029168310173830906
90-94	0.00244524755948283
95-99	0.0020493503355665625
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3435235.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.833425743294217
#Duplication Level	Relative count
1	100.0
2	9.124572551341071
3	2.6065709013735034
4	1.666341291383013
5	1.308638398901701
6	1.1033370908930606
7	0.9402296099904907
8	0.7739733360203034
9	0.7223331297113816
10++	7.705726395072769
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	775305	4.8397007	11.179712	85-89
GAGCC	747110	4.663699	11.093031	90-94
CGAGC	731410	4.5656943	10.920015	85-89
TCTCT	1871210	4.4121237	8.93845	90-94
CTCCG	809905	4.3416457	9.656052	95-97
GTCTC	1149295	4.3376884	7.1208997	70-74
CTGTC	1098325	4.145317	6.753465	65-69
AGCCC	711215	3.9394777	9.87717	90-94
GCCCA	704245	3.9008703	9.915307	90-94
CCACG	633710	3.5101712	9.687335	95-97
TGTCT	1300905	3.4568381	5.374593	90-94
TCTCC	1018515	3.4110336	6.613295	95-97
CCCAC	680495	3.3446786	8.704384	90-94
ACATC	1322080	3.3282635	8.639547	95-97
CTCTT	1409360	3.3231282	5.092705	60-64
CATCT	1352510	3.29521	8.520378	95-97
TCCGA	844265	3.2924793	7.2026134	85-89
ATCTC	1337775	3.25931	8.376259	95-97
GAGAC	695920	3.1603072	8.436073	95-97
CGAGA	694145	3.1522465	8.348422	85-89
ACACA	1197460	3.1148593	6.255428	6
ACGAG	666930	3.028658	8.274604	95-97
TACAC	1111675	2.798581	7.577215	5
CACAT	1097245	2.762254	5.0090175	95-97
AGAAG	821535	2.7140636	5.8095093	5
CACGA	657015	2.6475062	7.202392	95-97
CTCTC	767880	2.5716505	6.5629964	90-94
AGACC	624410	2.5161214	7.25239	85-89
GACCT	620960	2.4216309	6.939699	90-94
CCTCT	683940	2.290533	6.3453994	90-94
ACCTC	612895	2.1209092	6.18642	90-94
TATAC	1164900	2.0646918	5.622655	5
CAAAG	699165	2.0495822	5.125182	4
AGAGA	610055	2.0154078	5.7758727	8
GAGAG	388025	1.9858111	6.714349	7
CTTTG	686755	1.824884	7.735209	9
TGGAG	357915	1.7727222	5.0933013	5
AAGAG	519110	1.7149575	5.2713246	5
TCTCG	442490	1.6700532	5.9962616	90-94
GCTTT	575665	1.5296893	5.0651746	1
AGGAG	294205	1.5056647	5.1337724	7
TTGAG	481345	1.4894146	5.0437636	9
CTCGT	385455	1.4547908	5.8524213	90-94
GTATG	462115	1.4299116	5.135044	95-97
GTCTT	500505	1.32997	5.8666344	1
GAGTC	298545	1.3120861	6.35629	9
AAGAC	441980	1.2956519	6.019176	5
GCCGT	206475	1.247372	8.423402	95-97
TGCCG	204205	1.2336583	8.907696	95-97
GTGTT	408140	1.222224	6.197607	1
GATTG	393190	1.2166386	5.7233667	7
ATGCC	305135	1.1899709	6.0357003	95-97
CCATG	302670	1.1803577	5.0143623	9
TATGA	590500	1.1794913	5.5762773	4
CCGTC	209875	1.1250737	6.8723006	95-97
GACTT	407955	1.1201162	5.674977	7
GTTCT	413540	1.0988818	5.124447	1
TCATA	599190	1.0620162	5.290822	2
GTGTA	342005	1.058258	9.532261	1
TGGAC	234995	1.0327879	6.562981	5
GAGTA	297815	0.9521889	5.423184	1
GGACT	215700	0.9479877	6.241225	6
AGACT	332650	0.9437476	5.3796506	6
GTCCA	239085	0.9323878	6.3066406	1
GTATA	361850	0.7227755	7.079308	1
>>END_MODULE