##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-146_CTCTCTAC-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4089092	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73325178303643	33.0	31.0	34.0	30.0	34.0
2	31.930584344886345	33.0	31.0	34.0	30.0	34.0
3	32.0743541597988	34.0	31.0	34.0	30.0	34.0
4	35.614411952580184	37.0	35.0	37.0	33.0	37.0
5	35.485634463592405	37.0	35.0	37.0	33.0	37.0
6	35.616768465957726	37.0	35.0	37.0	33.0	37.0
7	35.6355545436493	37.0	35.0	37.0	33.0	37.0
8	35.68933958932692	37.0	35.0	37.0	33.0	37.0
9	37.506526387765305	39.0	38.0	39.0	35.0	39.0
10-14	37.63670697553393	39.2	37.2	39.4	34.6	39.4
15-19	38.69565869391053	40.0	38.2	41.0	34.6	41.0
20-24	38.59846181010356	40.0	38.0	41.0	34.0	41.0
25-29	38.42037552591138	40.0	38.0	41.0	34.0	41.0
30-34	38.15871127379868	40.0	38.0	41.0	33.4	41.0
35-39	37.848847323562296	40.0	38.0	41.0	32.8	41.0
40-44	37.817125513439166	40.0	37.8	41.0	32.8	41.0
45-49	37.67415127857235	40.0	37.2	41.0	32.4	41.0
50-54	36.958301891960375	39.2	36.4	40.2	31.4	40.6
55-59	37.59862634540872	40.0	36.6	41.0	32.2	41.0
60-64	37.15378264905754	39.2	35.6	41.0	31.8	41.0
65-69	36.480300712236364	38.2	35.0	40.4	31.0	41.0
70-74	35.60031963086182	36.6	34.8	39.4	30.2	41.0
75-79	34.56322572346134	35.4	34.0	37.6	29.0	39.6
80-84	33.469791239717765	35.0	33.4	36.4	27.4	38.2
85-89	32.423800687291944	35.0	32.8	35.4	25.0	36.6
90-94	31.535695895323464	35.0	31.6	35.0	21.8	36.0
95-99	30.698364967088047	34.4	30.6	35.0	18.4	35.0
100-101	29.51788049767528	33.5	28.5	35.0	13.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	106.0
7	445.0
8	899.0
9	1308.0
10	1481.0
11	1841.0
12	2601.0
13	3405.0
14	4242.0
15	5129.0
16	6060.0
17	7120.0
18	8258.0
19	9731.0
20	11110.0
21	13135.0
22	14922.0
23	17645.0
24	20579.0
25	24285.0
26	28969.0
27	34963.0
28	42959.0
29	52817.0
30	66964.0
31	85963.0
32	113917.0
33	153810.0
34	217659.0
35	325464.0
36	539444.0
37	939956.0
38	1099079.0
39	232625.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.035480456306956	23.62260124123985	10.93608683835137	24.405831464101826
2	15.032015229924003	24.529703684821598	39.665884001485416	20.772397083768983
3	15.232597279205779	28.68597850644973	33.52885229764546	22.552571916699037
4	11.218942171669669	18.87233275904793	44.619896530779634	25.288828538502766
5	10.8537431150242	39.05154603866962	38.74905356428607	11.345657282020106
6	24.681770493246567	43.98358534909257	20.023257349542554	11.311386808118307
7	22.723206446857493	34.53070655323886	27.718899835708044	15.027187164195603
8	20.655092703551134	38.10126712298615	25.24304539945238	16.00059477401033
9	22.969012513847893	15.356450244187553	25.39107914513569	36.283458096828866
10-14	20.246771161420234	26.103958034355472	33.16482065290576	20.484450151318523
15-19	19.657686714147342	31.624307616997015	29.008979593753704	19.70902607510194
20-24	19.577680645485113	30.242648855934267	30.44578654164401	19.73388395693661
25-29	19.555120741454342	30.632822447416014	30.062878918902847	19.749177892226797
30-34	19.40632282183967	30.492818861436994	30.18292427929994	19.9179340374234
35-39	19.542237365866736	30.645766872005876	30.092886889301717	19.719108872825668
40-44	19.260128971234618	30.086077679179134	30.694598139638906	19.959195209947335
45-49	19.134419598259473	29.550570926691183	31.148114971204077	20.16689450384527
50-54	18.544731856557377	29.404354923187604	31.509327072958428	20.541586147296595
55-59	18.403538930460595	29.431813151593367	31.483719780215747	20.68092813773029
60-64	18.367331465400856	28.85101607961149	31.4366285644965	21.345023890491156
65-69	18.89497582561196	28.636610692927377	30.63114629358254	21.83726718787812
70-74	19.119021412113472	28.639496715048164	30.112789053747974	22.12869281909039
75-79	19.772148068536954	29.31159628176812	29.26307302022036	21.653182629474564
80-84	20.58633044820345	29.403744498908583	28.751038975472355	21.25888607741561
85-89	21.19886980606275	29.25627817175385	28.599162054417988	20.945689967765414
90-94	21.762348702563166	28.804124436499702	28.70150463550062	20.732022225436513
95-99	22.760052151452875	28.131902243516215	28.191417393881117	20.916628211149792
100-101	23.560625894738465	27.413912204076734	28.210569540048635	20.81489236113616
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.44131192040878
2	35.804412313692985
3	37.785169195904814
4	36.50777071017243
5	22.199400397044307
6	35.99315730136487
7	37.7503936110531
8	36.655687477561465
9	59.25247061067675
10-14	40.73122131273876
15-19	39.36671278924928
20-24	39.311564602421726
25-29	39.30429863368114
30-34	39.324256859263066
35-39	39.261346238692404
40-44	39.21932418118196
45-49	39.30131410210474
50-54	39.086318003853975
55-59	39.08446706819088
60-64	39.71235535589201
65-69	40.732243013490084
70-74	41.247714231203865
75-79	41.42533069801152
80-84	41.84521652561906
85-89	42.14455977382816
90-94	42.49437092799968
95-99	43.676680362602674
100-101	44.375518255874624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	10.5
2	15.0
3	23.5
4	31.5
5	41.0
6	61.0
7	73.5
8	87.5
9	119.5
10	173.5
11	231.5
12	321.5
13	465.5
14	672.0
15	951.0
16	1337.0
17	1944.5
18	2783.5
19	3935.5
20	5655.5
21	8188.5
22	11861.0
23	16533.5
24	21851.5
25	28467.0
26	36647.0
27	45441.0
28	55779.0
29	67032.0
30	78621.5
31	91394.0
32	104485.0
33	118803.5
34	135278.0
35	153861.5
36	174940.5
37	194658.5
38	209368.0
39	217106.5
40	217513.0
41	212443.0
42	203223.0
43	193154.5
44	182957.5
45	170793.5
46	157441.0
47	142767.5
48	126697.5
49	109980.5
50	94445.0
51	82520.0
52	71389.5
53	60892.5
54	51931.5
55	43705.0
56	36619.0
57	30076.0
58	24105.5
59	19395.0
60	15489.0
61	12239.5
62	9560.5
63	6629.5
64	4526.0
65	3610.0
66	2782.5
67	2075.5
68	1642.5
69	1564.0
70	1546.0
71	1288.0
72	1095.5
73	906.0
74	712.0
75	562.0
76	396.0
77	284.0
78	190.0
79	120.0
80	86.5
81	67.5
82	62.5
83	49.5
84	44.5
85	44.0
86	32.5
87	26.5
88	25.0
89	21.5
90	16.0
91	16.5
92	14.0
93	9.5
94	10.5
95	11.0
96	11.0
97	9.0
98	5.5
99	2.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03435970626241718
2	0.033014664380258504
3	0.02856379851566069
4	0.027218756633502008
5	0.033919510737347065
6	0.0015162290307970572
7	7.33659208450189E-4
8	0.003521564200560907
9	5.380167528634719E-4
10-14	0.007331701023112222
15-19	0.024469980132508635
20-24	0.020307686889901228
25-29	0.0022107597481299027
30-34	0.002166740195622891
35-39	0.006064916123188229
40-44	0.013509111558262813
45-49	0.008642505475543226
50-54	0.0053997317741933904
55-59	0.004230768102062756
60-64	0.01066251382947608
65-69	0.014462868529248059
70-74	0.0183903908251514
75-79	0.008104488722679755
80-84	0.010975541758414828
85-89	0.009239214965082712
90-94	0.009963092050753566
95-99	0.009420184236500427
100-101	0.006297241539197455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4089092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.325484035098686
#Duplication Level	Relative count
1	100.0
2	9.239737957210076
3	2.814750305456625
4	1.828190396963631
5	1.4219980762731692
6	1.2120778849403384
7	0.9904593547716224
8	0.9209192294694153
9	0.8331816882002756
10++	10.720227727662673
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	1112105	5.5375786	13.618951	80-84
GACGC	1077430	5.494847	13.6620865	75-79
GTCTC	1653385	5.4443197	9.426706	60-64
CGCTG	1069460	5.325234	13.460275	75-79
CTGTC	1590475	5.2371674	9.056999	55-59
GCCGA	981570	5.0059648	13.756308	80-84
CTGCC	1033180	4.9694304	13.175347	80-84
CGACG	953740	4.864033	13.775907	85-89
TGCCG	969160	4.8258038	13.383694	80-84
CCGAC	945365	4.657174	13.179946	80-84
TCTCT	2063790	4.4939747	7.29879	60-64
CTCTT	1942125	4.229045	6.936765	60-64
TGTCT	1824350	4.1126037	6.712984	55-59
TGACG	1156340	4.037301	9.573247	75-79
CTGAC	1196730	4.036065	9.175344	75-79
ACACA	1668730	3.9041662	7.1770477	70-74
GAGAG	997670	3.6934175	10.701762	95-97
ACGCT	1085385	3.6605458	9.083091	75-79
GACGA	1015620	3.6318607	10.1107	85-89
ATCTG	1559950	3.6017346	7.145058	70-74
TACAC	1573950	3.5953472	6.6772323	5
CACAT	1547285	3.5344367	6.6104894	70-74
CATCT	1560230	3.4797351	6.7280746	70-74
ACATC	1498685	3.4234202	6.701116	70-74
TCTGA	1450040	3.347966	6.6874895	70-74
AGAGA	1280905	3.2117836	7.657441	90-94
AGAGG	854900	3.164877	10.524596	95-97
TATAC	1643985	2.5709095	5.346534	5
ACGAA	1044085	2.528842	6.869229	85-89
GAGGT	673800	2.4354548	8.520845	95-97
CGAAT	1019510	2.4109318	6.600143	85-89
TAGAG	943885	2.3107667	6.970577	90-94
AGGTG	632635	2.2866638	8.867383	95-97
GGTGT	600485	2.1191363	7.1822376	95-97
GGTGG	389855	2.0804842	10.43378	95-97
CTTTG	788705	1.7779653	7.983779	9
CTCGG	339090	1.6884537	8.934395	95-97
GTGTA	692405	1.6550258	7.9263186	1
GCTTT	658305	1.4840066	5.0280843	1
TCTCG	428075	1.4095792	5.76826	95-97
CGGTG	269805	1.3908094	8.439212	95-97
GGTCG	240605	1.2402875	5.0005207	95-97
AAGAC	509040	1.2329279	5.4809384	5
GAGTC	345720	1.2070633	6.5339956	9
CGCCG	161125	1.1719202	5.19173	95-97
GTCTT	502920	1.1337247	6.2189465	1
GACTT	469925	1.0849997	5.6365566	7
GTTCT	477285	1.0759361	5.002933	1
GTGTT	454270	1.0601475	5.6117206	1
TCGGT	301625	1.028207	5.3818517	95-97
TGGAC	277775	0.9698369	6.3139176	5
GTCCA	276435	0.93229866	6.8401732	1
AGACT	381565	0.9023228	5.0459075	6
GGACT	255765	0.8929902	6.1157045	6
GTATA	414630	0.6712638	6.7821918	1
>>END_MODULE