##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-146_CTCTCTAC-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4089092	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44643407387264	34.0	31.0	34.0	31.0	34.0
2	32.62843193550059	34.0	31.0	34.0	31.0	34.0
3	32.73679242237641	34.0	31.0	34.0	31.0	34.0
4	36.26090046396608	37.0	37.0	37.0	35.0	37.0
5	35.984562098382725	37.0	35.0	37.0	35.0	37.0
6	36.06864384562636	37.0	36.0	37.0	35.0	37.0
7	36.06788719842938	37.0	36.0	37.0	35.0	37.0
8	36.092204088340395	37.0	36.0	37.0	35.0	37.0
9	37.966354877806616	39.0	38.0	39.0	35.0	39.0
10-14	38.153889323106455	39.4	38.2	39.4	35.2	39.4
15-19	39.276207529691185	40.2	39.0	41.0	36.0	41.0
20-24	39.211301482089425	40.0	39.0	41.0	36.0	41.0
25-29	39.01480807964213	40.0	38.8	41.0	35.8	41.0
30-34	38.81331655047135	40.0	38.0	41.0	35.0	41.0
35-39	38.58090485613921	40.0	38.0	41.0	34.6	41.0
40-44	38.339726325551	40.0	38.0	41.0	33.8	41.0
45-49	38.16643866168822	40.0	38.0	41.0	33.4	41.0
50-54	38.125025751438216	40.0	37.4	41.0	33.4	41.0
55-59	37.756421816872795	39.8	36.6	41.0	33.0	41.0
60-64	37.198603797615704	39.0	35.6	40.8	32.0	41.0
65-69	36.454004458691564	37.6	35.0	40.0	31.0	41.0
70-74	35.84350731164768	36.6	35.0	39.2	31.0	41.0
75-79	34.62665833881947	35.2	33.6	37.4	30.2	39.2
80-84	34.41667426411536	35.0	34.0	36.4	31.0	38.2
85-89	33.80399624170843	35.0	34.0	35.4	30.2	36.6
90-94	33.40477739312297	35.0	34.0	35.0	29.8	36.0
95-99	33.14393527951927	35.0	34.0	35.0	29.0	35.2
100-101	32.77393807720638	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	17.0
14	46.0
15	150.0
16	382.0
17	805.0
18	1414.0
19	2377.0
20	3497.0
21	4748.0
22	6992.0
23	9460.0
24	12663.0
25	16435.0
26	21016.0
27	27240.0
28	34370.0
29	43483.0
30	54955.0
31	69083.0
32	89376.0
33	119181.0
34	171821.0
35	270501.0
36	505900.0
37	1007434.0
38	1335586.0
39	279881.0
40	275.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.641110701620484	23.671300051771897	11.227898816631862	24.45969042997576
2	15.188495392541384	24.41882136326771	39.53604520680489	20.856638037386013
3	15.378108391789668	28.40251576633639	33.737636619572264	22.481739222301673
4	11.406493177458467	18.83819195068245	44.41797836781369	25.3373365040454
5	10.734485797825043	39.179822806627975	38.7972684400351	11.288422955511884
6	24.67696985051938	44.221700074246314	19.888718571262277	11.212611503972031
7	22.687457264351107	34.55581336883592	27.705930803219886	15.05079856359309
8	20.656639664747086	38.2029800258835	25.171236059252273	15.96914425011714
9	22.912250445820245	15.306454342431033	25.34704526090389	36.43424995084483
10-14	20.17734499492797	26.108744924301043	33.20808140291292	20.50582867785807
15-19	19.61801820132486	31.66002900923272	29.003089475948286	19.718863313494136
20-24	19.514320123838615	30.26926114481266	30.470099292654712	19.74631943869401
25-29	19.55019534551072	30.629119432383888	30.06521626935936	19.75546895274603
30-34	19.411613705792472	30.497807532212494	30.180185115636863	19.91039364635817
35-39	19.524641789072213	30.63946456854269	30.075141467328137	19.760752175056957
40-44	19.26448001958957	30.087300614043322	30.664109465913164	19.984109900453948
45-49	19.1260770014108	29.540298338015614	31.136934731769333	20.196689928804254
50-54	18.506113797628608	29.36867521196666	31.537578031522223	20.58763295888251
55-59	18.225424944526395	29.32175092308402	31.452473644012922	21.00035048837666
60-64	18.023329217514128	28.78838712607247	31.191605020725248	21.996678635688145
65-69	18.324812612399164	28.799116841599925	30.12393273242274	22.752137813578162
70-74	18.283063251028437	28.57626882205559	29.517029213340695	23.62363871357528
75-79	18.099656089972925	28.55534653583024	29.16957464727738	24.175422726919454
80-84	17.63278754369621	28.252430874137453	29.109390399993192	25.005391182173142
85-89	17.476054015590496	27.938762218042722	29.281458078948724	25.303725687418062
90-94	17.558668375062044	27.14427880261284	29.60322263001716	25.693830192307953
95-99	17.602661035030756	26.483042754217824	29.79765641213487	26.116639798616546
100-101	17.6823384852112	25.65262968559153	30.50599381305114	26.15903801614613
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.10080113159624
2	36.045133429927404
3	37.85984761409134
4	36.74382968150386
5	22.022908753336925
6	35.88958135449141
7	37.7382558279442
8	36.62578391486423
9	59.34650039666508
10-14	40.68317367278604
15-19	39.33688151481899
20-24	39.26063956253263
25-29	39.30566429825675
30-34	39.32200735215064
35-39	39.28539396412917
40-44	39.248589920043514
45-49	39.32276693021505
50-54	39.093746756511116
55-59	39.225775432903056
60-64	40.02000785320227
65-69	41.07695042597733
70-74	41.90670196460371
75-79	42.27507881689238
80-84	42.63817872586935
85-89	42.77977970300855
90-94	43.252498567370004
95-99	43.71930083364731
100-101	43.84137650135733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	3.5
6	7.0
7	17.5
8	28.5
9	43.0
10	78.5
11	114.5
12	200.5
13	326.5
14	494.0
15	775.0
16	1183.0
17	1758.0
18	2624.5
19	3790.0
20	5521.5
21	8158.0
22	11689.0
23	16422.5
24	22152.5
25	28754.5
26	36203.5
27	44740.5
28	55091.5
29	65778.5
30	76921.5
31	89313.5
32	101908.5
33	115732.0
34	131745.0
35	150043.0
36	170272.5
37	188732.5
38	202863.0
39	210199.5
40	209619.0
41	205068.5
42	197497.0
43	189162.5
44	182300.5
45	174390.5
46	165079.5
47	152602.5
48	136467.5
49	118706.0
50	102043.5
51	88710.5
52	76354.5
53	64738.5
54	54711.0
55	46023.5
56	38585.0
57	31507.0
58	24987.5
59	19875.5
60	15627.5
61	12255.5
62	9616.5
63	6693.5
64	4533.0
65	3544.5
66	2743.0
67	2002.5
68	1519.5
69	1503.5
70	1554.0
71	1253.0
72	1059.0
73	935.0
74	698.5
75	526.0
76	362.5
77	218.0
78	119.0
79	65.0
80	42.5
81	36.0
82	27.5
83	18.5
84	8.5
85	3.5
86	1.5
87	1.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.4455306948339632E-5
2	0.012325474701963175
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001198310040468642
20-24	6.896396559431776E-4
25-29	0.01699643832909604
30-34	0.006720318349403731
35-39	0.04112893522571759
40-44	0.049370373667308044
45-49	0.05013337924409624
50-54	0.05032413063829329
55-59	0.040414840262826074
60-64	0.051277887609278545
65-69	0.043085359781584766
70-74	0.004049798830645043
75-79	0.0014037346188346949
80-84	1.8096927141771326E-4
85-89	0.0034041787272088768
90-94	0.002660737395979352
95-99	0.0023623826512096085
100-101	1.2227653474169816E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4089092.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.72531745360122
#Duplication Level	Relative count
1	100.0
2	9.458480928430241
3	2.7287968634818744
4	1.8474665831653523
5	1.3536345904195288
6	1.1866319614328305
7	1.0313717047967592
8	0.9465656822644513
9	0.8532790574789126
10++	11.064576524388254
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	1173540	6.003707	14.160686	75-79
GAGCC	1133450	5.798611	14.039639	75-79
CGAGC	1114450	5.7014093	13.877911	75-79
CTCCG	1218135	5.1265936	11.492078	75-79
TCTCT	2667110	5.0538306	10.182226	80-84
GTCTC	1655810	5.0493584	8.701464	60-64
CTGTC	1596435	4.868295	8.442168	55-59
AGCCC	1086505	4.7666717	11.915202	80-84
GCCCA	1066445	4.6786647	12.050489	80-84
CCACG	974745	4.2763624	11.790124	80-84
TGTCT	1830420	4.044535	6.612986	55-59
ACATC	1955330	4.0262423	10.431021	95-97
TCCGA	1258755	4.0014453	8.957243	75-79
CGAGA	1032110	3.9883182	10.922419	85-89
GAGAC	1027070	3.9688425	10.967763	85-89
CATCT	1989970	3.9307585	10.191862	95-97
CCCAC	1033500	3.8882654	10.298203	80-84
ATCTC	1968220	3.8877962	10.142058	95-97
ACGAG	1004860	3.8830175	10.612501	85-89
TCTCC	1458205	3.8133454	7.646809	70-74
CTCTT	1948880	3.692877	6.073147	60-64
ACACA	1676065	3.5976648	6.606293	70-74
CACGA	994220	3.294642	9.031515	80-84
TACAC	1580840	3.2551258	6.204765	5
CACAT	1553405	3.1986341	6.000886	70-74
AGACC	938860	3.11119	9.17086	85-89
GACCT	935180	2.9728355	8.879519	85-89
CTCTC	1071395	2.8018005	7.7717133	90-94
AGAAG	949665	2.7718928	5.8220325	5
CCTCT	988185	2.5841985	7.595712	90-94
ACCTC	907640	2.474294	7.5405393	90-94
TATAC	1652605	2.4657052	5.320581	5
AGAGA	698970	2.0401614	6.12488	8
GAGAG	442675	1.9947426	6.997825	7
CAAAG	794105	1.9876772	5.0254793	4
TCTCG	642835	1.9603118	7.9784966	90-94
TGGAG	413360	1.786818	5.2647266	5
CTCTA	903040	1.7837615	5.617911	95-97
CTACA	854680	1.7598814	5.7741194	95-97
CTTTG	789340	1.7441425	7.899967	9
AAGAG	594965	1.7365905	5.4286137	5
CTCGT	562815	1.7162926	7.9048605	90-94
GTATG	616985	1.6572217	7.235956	95-97
TCTAC	824385	1.6283956	5.5317125	95-97
AGGAG	346235	1.5601733	5.197573	7
TGCCG	302600	1.485048	12.276223	95-97
GCCGT	298935	1.4670615	11.7654085	95-97
ATGCC	429450	1.365175	8.137341	95-97
GTCTT	606895	1.3410083	5.77846	1
GAGTC	347860	1.2894909	6.655364	9
AAGAC	507510	1.2703184	5.680819	5
ATGAG	448885	1.256872	5.1641736	7
CCGTC	297750	1.2530986	9.213378	95-97
GATTG	454745	1.2214451	5.3224916	7
TCGTA	520255	1.1983513	5.874641	95-97
TGAGT	442405	1.1882998	5.355049	8
GTGTT	454805	1.1718748	5.9786305	1
TATGC	502260	1.1569017	5.952821	95-97
CGTAT	476730	1.0980961	5.933834	95-97
GACTT	473160	1.0898732	5.7068067	7
ATGTG	401045	1.0772069	5.1050076	7
GTGTA	389605	1.0464789	8.578108	1
TGGAC	276220	1.0239269	6.849471	5
GAGTA	340330	0.95291954	5.060334	1
CGTCT	309755	0.94459146	6.1219344	95-97
GGACT	253125	0.9383154	6.4307027	6
AGACT	383500	0.9208381	5.1538496	6
GGATA	315915	0.88455784	5.262639	1
GTCCA	277840	0.8832232	6.324716	1
GTATA	412805	0.71821606	7.0910687	1
>>END_MODULE