##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-136_GGACTCCT-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1009776	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62924945730538	33.0	31.0	34.0	30.0	34.0
2	31.832695568126	33.0	31.0	34.0	30.0	34.0
3	31.97459832675762	34.0	31.0	34.0	30.0	34.0
4	35.499078013341574	37.0	35.0	37.0	33.0	37.0
5	35.372535096892776	37.0	35.0	37.0	33.0	37.0
6	35.51120446514871	37.0	35.0	37.0	33.0	37.0
7	35.52981552344282	37.0	35.0	37.0	33.0	37.0
8	35.58038515472739	37.0	35.0	37.0	33.0	37.0
9	37.37915339639682	39.0	37.0	39.0	34.0	39.0
10-14	37.50175306206525	39.2	37.2	39.4	34.0	39.4
15-19	38.54637008603889	40.0	38.0	41.0	34.0	41.0
20-24	38.445374617737	40.0	38.0	41.0	34.0	41.0
25-29	38.26492885550855	40.0	38.0	41.0	33.6	41.0
30-34	37.998728628923644	40.0	38.0	41.0	33.0	41.0
35-39	37.68936496807213	40.0	37.6	41.0	32.2	41.0
40-44	37.64959991126745	40.0	37.6	41.0	31.8	41.0
45-49	37.491585460537785	40.0	37.0	41.0	31.4	41.0
50-54	36.814126499342436	39.2	36.4	40.2	31.0	40.6
55-59	37.457240615740524	40.0	36.6	41.0	32.0	41.0
60-64	37.01315044128599	39.0	35.4	41.0	31.0	41.0
65-69	36.350823350921395	38.2	35.0	40.2	30.4	41.0
70-74	35.5131559870704	36.6	34.6	39.4	29.4	41.0
75-79	34.54730831392309	35.6	34.0	38.0	29.0	39.6
80-84	33.60848901142431	35.0	33.6	36.4	27.8	38.2
85-89	32.811688730966075	35.0	33.0	35.6	26.2	36.8
90-94	32.22471102501941	35.0	32.4	35.0	25.2	36.0
95-99	31.726468840614153	35.0	32.0	35.0	24.2	35.2
100-101	30.84520329261143	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	27.0
7	95.0
8	211.0
9	280.0
10	332.0
11	466.0
12	728.0
13	1032.0
14	1269.0
15	1538.0
16	1722.0
17	2049.0
18	2282.0
19	2708.0
20	3076.0
21	3459.0
22	4074.0
23	4579.0
24	5290.0
25	6349.0
26	7253.0
27	8702.0
28	10445.0
29	12551.0
30	15665.0
31	19726.0
32	25607.0
33	34508.0
34	49198.0
35	75070.0
36	128715.0
37	234500.0
38	286361.0
39	59858.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.407653976085044	23.73544942094887	10.797495566717192	24.059401036248897
2	15.15192041551628	23.384480352035727	40.243579875238126	21.220019357209868
3	15.921313709970608	27.436088192202497	32.785888138115084	23.85670995971181
4	11.144516251738498	18.252447765806963	44.373506674644275	26.229529307810267
5	10.680797567709742	39.31766689551118	39.002535121688815	10.999000415090265
6	25.660436405482944	43.721459491418166	19.42334184039796	11.194762262700928
7	24.068550263921487	33.78997197381582	26.500985372906698	15.640492389355993
8	21.10602181738954	38.73968180085071	24.1108398655106	16.04345651624915
9	22.631139033916703	15.147340775306803	25.62410375452579	36.597416436250704
10-14	20.347095258563485	26.118190263584808	32.83192729678497	20.70278718106674
15-19	19.580937037337147	31.934878466708682	28.778493791142512	19.705690704811666
20-24	19.530343195045894	30.24168489418399	30.499759200645194	19.72821271012492
25-29	19.512017342923833	30.75164223980415	29.946055901020944	19.79028451625107
30-34	19.406320611509535	30.52823747333995	29.983033430835977	20.08240848431454
35-39	19.539408436202784	30.765005642212984	29.97590793873331	19.71967798285092
40-44	19.317532612114917	30.438396521163106	30.387506329083834	19.856564537638143
45-49	19.459190066696497	30.32380019693403	30.315243015051724	19.901766721317742
50-54	19.165147930904507	29.942199897912435	30.809686711601834	20.082965459581224
55-59	19.411126446325515	29.858778257025236	30.82287642493369	19.907218871715564
60-64	19.066960866053904	29.500659333867773	31.10588028351059	20.32649951656773
65-69	19.46438391333218	29.627286226618722	30.45009801793007	20.458231842119027
70-74	19.451242729071154	29.355158105186334	30.478430188583555	20.715168977158953
75-79	19.851935045538642	29.639058247465567	30.041042218815246	20.467964488180552
80-84	19.975298688770412	29.409377188230135	29.91552626965631	20.699797853343146
85-89	20.231728313774706	29.373434656283997	29.769539411601116	20.625297618340184
90-94	20.256174434573467	28.910019636091505	30.191763379650055	20.642042549684977
95-99	20.702290112625825	28.732203760480058	29.6610924891421	20.904413637752015
100-101	21.038560385851454	28.022857935159923	30.19109352639111	20.747488152597516
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.46705501233394
2	36.37193977272615
3	39.77802366968242
4	37.37404555954876
5	21.679797982800007
6	36.855198668183874
7	39.70904265327748
8	37.14947833363869
9	59.22855547016741
10-14	41.049882439630224
15-19	39.28662774214881
20-24	39.258555905170816
25-29	39.302301859174904
30-34	39.48872909582408
35-39	39.25908641905371
40-44	39.17409714975306
45-49	39.36095678801424
50-54	39.248113390485734
55-59	39.31834531804108
60-64	39.39346038262164
65-69	39.922615755451204
70-74	40.16641170623011
75-79	40.319899533719195
80-84	40.67509654211356
85-89	40.857025932114894
90-94	40.89821698425845
95-99	41.60670375037785
100-101	41.786048538448966
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	2.0
4	5.0
5	9.0
6	12.5
7	14.5
8	19.0
9	31.0
10	46.0
11	58.0
12	75.5
13	109.5
14	160.0
15	217.0
16	301.5
17	462.0
18	688.0
19	959.5
20	1375.5
21	2013.5
22	2866.0
23	4025.5
24	5407.0
25	7047.0
26	9173.0
27	11514.5
28	14238.0
29	17225.5
30	20418.5
31	23890.0
32	27723.5
33	31996.0
34	36643.0
35	41892.0
36	48047.0
37	53850.5
38	57475.5
39	58059.5
40	56192.5
41	52473.0
42	47974.0
43	44221.0
44	41232.5
45	38093.0
46	34739.0
47	31411.0
48	27703.5
49	23755.0
50	20282.5
51	17842.5
52	15522.5
53	13226.5
54	11391.5
55	9727.0
56	8357.5
57	7156.5
58	5780.5
59	4795.0
60	3992.5
61	3253.5
62	2652.5
63	1907.0
64	1363.5
65	1129.5
66	913.5
67	725.5
68	599.0
69	584.5
70	563.5
71	453.0
72	388.0
73	336.5
74	250.5
75	182.5
76	137.0
77	100.5
78	69.5
79	54.0
80	42.5
81	30.0
82	17.5
83	16.0
84	15.5
85	10.5
86	6.5
87	9.5
88	8.0
89	4.0
90	4.0
91	3.0
92	3.0
93	3.0
94	4.0
95	3.0
96	2.0
97	3.5
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036245662404335216
2	0.03297761087607549
3	0.029610527483323035
4	0.028125049515932248
5	0.035354375623900744
6	0.0026738603413034175
7	5.94191186956315E-4
8	0.003069987799274295
9	7.922549159417535E-4
10-14	0.008120612888402974
15-19	0.02640189507375893
20-24	0.021965267544485113
25-29	0.0026738603413034175
30-34	0.002535215731013611
35-39	0.0065162966836209224
40-44	0.01497361791129914
45-49	0.009685316347387935
50-54	0.005407139801302467
55-59	0.0044366275292738195
60-64	0.011943242857821933
65-69	0.01632045126840012
70-74	0.020559015068688503
75-79	0.008318676617388412
80-84	0.011190600687677266
85-89	0.009269382516518515
90-94	0.010358733025938427
95-99	0.009546671737098129
100-101	0.00604094373405587
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1009776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.118316289179198
#Duplication Level	Relative count
1	100.0
2	5.844314785826099
3	2.116977372989896
4	1.3263127935107444
5	0.9028034723210474
6	0.685356482140316
7	0.5288174185285328
8	0.34609363882168775
9	0.30966272947203644
10++	1.7862530240500925
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	227160	3.1149921	5.219348	90-94
TCTCT	337650	3.0400398	4.3963337	80-84
GCTGC	144045	3.0084019	6.8168683	90-94
CTCTT	309535	2.7869055	5.035512	1
GACGC	123720	2.630612	6.7481394	95-97
CGCTG	123635	2.582136	6.3306665	85-89
ACACA	271545	2.579835	8.067344	6
CTGCC	124505	2.5429127	6.519806	90-94
GCCGA	117370	2.4955945	6.680208	90-94
GAAGG	167165	2.4843624	5.4505205	85-89
CGACG	116460	2.4762452	6.9543996	95-97
CCGAC	112890	2.347358	6.4647245	95-97
TGCCG	110180	2.3011262	6.458299	90-94
CAAAG	236135	2.294053	5.33921	4
TACAC	243440	2.271762	10.2211275	5
CACAT	237845	2.2195497	5.5529084	7
CTTTG	226915	2.089146	7.8848367	9
GACGA	135815	1.9738959	5.155372	95-97
ATACA	290570	1.853452	5.0619497	4
GAGAG	120895	1.7967098	6.1622705	7
GCTTT	190055	1.7497854	5.1402316	1
TGGAG	115040	1.6793425	5.2328568	5
CTCCA	121335	1.6565223	5.172066	1
CACCA	113280	1.5745037	5.068895	7
TATAC	247765	1.5523559	5.9263864	5
TTGAG	156165	1.4967921	5.8101087	9
GACTC	103500	1.4449228	5.2469997	7
GTGTA	149770	1.4354982	11.297594	1
GAGTC	99370	1.418576	7.282987	9
AAGAC	145760	1.4160595	5.9282675	5
CATGA	143185	1.3663485	5.145946	9
CCATG	97245	1.3575991	5.8765235	9
GTCTT	143540	1.3215344	6.872995	1
GACTT	136265	1.2772299	6.409275	7
TGAGT	131925	1.2644594	5.02933	8
AACAC	132970	1.263292	5.198322	5
TATGA	196755	1.2605793	6.4548316	4
GTGTT	133280	1.2547685	6.101083	1
TCATA	197080	1.2347921	6.506771	2
GATTG	125640	1.2042197	6.247153	7
GTTCT	127650	1.1752393	5.305469	1
ACATG	120475	1.1496373	5.77542	8
ACACC	81570	1.1337594	5.59452	6
GTCCA	79305	1.1071459	7.8350096	1
TGGAC	77195	1.1020125	7.395586	5
ACACT	114890	1.0721439	5.5844493	6
AGACT	108485	1.0352224	5.548524	6
GGACT	71585	1.0219257	6.895265	6
TGTAT	155080	0.9759352	5.1893344	2
GAGTA	99995	0.9757425	5.5804935	1
GTATG	100165	0.96004987	5.9276614	3
CATAC	99180	0.9255396	5.6264887	3
GTATA	118640	0.7601084	7.0918884	1
ATACT	120670	0.7560502	5.019445	6
>>END_MODULE