##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-136_GGACTCCT-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1009776	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44936698832216	34.0	31.0	34.0	31.0	34.0
2	32.63460311989986	34.0	31.0	34.0	31.0	34.0
3	32.74655072015972	34.0	31.0	34.0	31.0	34.0
4	36.255336827177516	37.0	37.0	37.0	35.0	37.0
5	35.98062936730523	37.0	35.0	37.0	35.0	37.0
6	36.07355195607739	37.0	36.0	37.0	35.0	37.0
7	36.076888339592145	37.0	36.0	37.0	35.0	37.0
8	36.09915169304875	37.0	36.0	37.0	35.0	37.0
9	37.9595801445073	39.0	38.0	39.0	35.0	39.0
10-14	38.1450636576825	39.2	38.2	39.4	35.2	39.4
15-19	39.278926019235946	40.0	39.0	41.0	36.0	41.0
20-24	39.211976517564295	40.0	39.0	41.0	36.0	41.0
25-29	39.0248072839917	40.0	38.8	41.0	36.0	41.0
30-34	38.829318779610524	40.0	38.0	41.0	35.0	41.0
35-39	38.6041395319358	40.0	38.0	41.0	34.6	41.0
40-44	38.37539394875695	40.0	38.0	41.0	34.0	41.0
45-49	38.20107172283754	40.0	38.0	41.0	33.4	41.0
50-54	38.16343050339877	40.0	37.6	41.0	33.6	41.0
55-59	37.789890629208855	39.8	36.8	41.0	33.0	41.0
60-64	37.252090958786894	39.0	35.6	40.8	32.0	41.0
65-69	36.524454532490374	37.8	35.0	40.0	31.0	41.0
70-74	35.949972073014216	36.8	35.0	39.2	31.0	41.0
75-79	34.75693579566162	35.2	34.0	37.4	30.4	39.2
80-84	34.543068165612965	35.0	34.0	36.6	31.0	38.4
85-89	33.90424252507487	35.0	34.0	35.8	30.2	36.8
90-94	33.486293989954206	35.0	34.0	35.0	30.0	36.0
95-99	33.205916361648526	35.0	34.0	35.0	29.2	35.6
100-101	32.850217275910694	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	0.0
12	0.0
13	6.0
14	21.0
15	30.0
16	66.0
17	168.0
18	249.0
19	493.0
20	749.0
21	1078.0
22	1475.0
23	2054.0
24	2907.0
25	3750.0
26	4779.0
27	6347.0
28	8177.0
29	10426.0
30	13186.0
31	16847.0
32	21857.0
33	29398.0
34	42624.0
35	68218.0
36	124462.0
37	245763.0
38	331960.0
39	72612.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.836977705946666	23.77804582402434	11.142075074075834	24.24290139595316
2	15.276868479672276	23.33268293069448	40.06681649038114	21.3236320992521
3	16.072277415981368	26.997175611224666	33.05871797309503	23.871828999698945
4	11.313895358970703	18.22295241716975	44.107505030818714	26.35564719304083
5	10.460042623314477	39.51153523157611	39.159575985168985	10.868846159940421
6	25.79651328611494	43.82853226854272	19.153752911536817	11.221201533805518
7	24.069694664955396	33.758378095736084	26.413283738175597	15.758643501132925
8	21.22104308282233	38.757803710922026	23.914907860753274	16.10624534550237
9	22.544901047360998	14.961734087560014	25.563194213370092	36.93017065170889
10-14	20.313990429560615	26.078437197952812	32.82221007431351	20.78536229817306
15-19	19.503060416259395	31.971095045320464	28.765536046718214	19.760308491701927
20-24	19.50606089595612	30.203407078971196	30.513656500571123	19.776875524501563
25-29	19.492954074703288	30.681193274833873	30.01812194236794	19.80773070809489
30-34	19.383580325484136	30.410370045524044	30.083226025103095	20.12282360388873
35-39	19.524724672123988	30.714842927232517	29.998527780271107	19.761904620372388
40-44	19.26377618142773	30.365630919474246	30.4742435804884	19.896349318609623
45-49	19.403671842752058	30.261184868505925	30.370907759253274	19.964235529488743
50-54	19.113700978512515	29.853301397272038	30.854682003744106	20.17831562047134
55-59	19.347976546922503	29.818522192982822	30.762320127652075	20.071181132442597
60-64	18.92085293048706	29.450662732901776	31.013196793009275	20.615287543601895
65-69	19.251803465149848	29.687577714213813	30.306021854599248	20.75459696603709
70-74	19.161238989902472	29.465634551418685	30.210544934587606	21.16258152409124
75-79	19.483865779670946	29.65057262087667	29.778187027697456	21.08737457175493
80-84	19.247709042278956	29.47979855298343	29.598637002212968	21.673855402524644
85-89	19.268059925224197	29.314642287546956	29.568529829112173	21.848767958116678
90-94	19.114170358679875	28.834187408491946	29.982217331229045	22.069424901599135
95-99	19.218639824432138	28.692471022587725	29.690419033584487	22.398470119395654
100-101	19.10524621821693	27.849520932881088	30.495456908717287	22.549775940184695
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.07987910189983
2	36.600500578924375
3	39.94410641568031
4	37.669542552011535
5	21.3288887832549
6	37.01771481992046
7	39.82833816608832
8	37.32728842832469
9	59.47507169906989
10-14	41.099352727733674
15-19	39.26336890796132
20-24	39.28293642045769
25-29	39.30068478279818
30-34	39.506403929372865
35-39	39.28662929249638
40-44	39.16012550003735
45-49	39.3679073722408
50-54	39.29201659898386
55-59	39.4191576793651
60-64	39.53614047408895
65-69	40.00640043118695
70-74	40.32382051399371
75-79	40.57124035142587
80-84	40.9215644448036
85-89	41.116827883340875
90-94	41.18359526027901
95-99	41.61710994382779
100-101	41.65502215840162
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.5
7	5.0
8	10.5
9	15.0
10	19.0
11	33.0
12	45.5
13	67.0
14	119.5
15	184.0
16	283.5
17	441.5
18	620.0
19	897.5
20	1321.0
21	1926.0
22	2856.5
23	4092.0
24	5464.0
25	7087.5
26	9195.5
27	11608.5
28	14221.5
29	16909.0
30	20142.5
31	23946.5
32	27510.5
33	31337.5
34	35945.5
35	41201.0
36	47669.5
37	53684.0
38	57138.5
39	57738.5
40	55483.0
41	51609.0
42	47579.0
43	44049.5
44	41158.5
45	38300.5
46	35050.5
47	31899.5
48	28734.0
49	24843.5
50	21096.5
51	18461.5
52	16168.5
53	13893.5
54	11828.5
55	10058.5
56	8645.5
57	7248.5
58	5792.0
59	4767.0
60	3937.5
61	3269.0
62	2677.0
63	1862.5
64	1340.5
65	1091.0
66	856.0
67	693.0
68	569.0
69	548.0
70	538.0
71	437.0
72	369.0
73	321.0
74	267.0
75	198.5
76	127.0
77	98.5
78	63.5
79	37.0
80	26.0
81	15.5
82	10.0
83	7.0
84	4.0
85	3.0
86	1.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01435962035144428
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011289632552169985
20-24	7.724485430432096E-4
25-29	0.016954255201153522
30-34	0.0067143604126063605
35-39	0.04117744925607263
40-44	0.04965457685664939
45-49	0.050129929806214446
50-54	0.05044683177259114
55-59	0.040345581594333796
60-64	0.05256611367273534
65-69	0.046445944447085295
70-74	0.0043375956647811
75-79	0.0015250907131878753
80-84	1.782573560868945E-4
85-89	0.0037235981049262407
90-94	0.002852117697390312
95-99	0.00237676474782526
100-101	9.903186449271919E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1009776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.79302536231884
#Duplication Level	Relative count
1	100.0
2	6.0369720222541154
3	2.174522125462436
4	1.3728707923311358
5	0.9526042232501758
6	0.7318928005763656
7	0.4968865966412982
8	0.4059717878197028
9	0.3041929452395519
10++	1.8823367964823403
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	146100	3.09415	7.078827	95-97
GTCTC	227050	3.0495944	5.1060514	90-94
GAGCC	141905	3.0053074	6.815254	95-97
CTCCG	153030	2.9975297	6.895478	95-97
CGAGC	134955	2.8581183	6.7858696	85-89
AGAAG	271745	2.8067327	5.483636	5
TCTCC	213760	2.7044764	5.114345	95-97
GCCCA	133205	2.6573448	6.62236	90-94
AGCCC	132010	2.6335056	6.287664	90-94
ACACA	272745	2.499589	8.269385	6
CCCAC	129235	2.4285312	6.1669793	90-94
ATCTC	274380	2.424283	5.665166	95-97
CCACG	116230	2.3187056	6.231558	90-94
CAAAG	233260	2.2694254	5.0380435	4
GACGG	99110	2.2282941	6.6217704	85-89
TACAC	244665	2.2016242	10.144001	5
GACTC	157620	2.1561172	5.677405	7
GAGAC	145690	2.154741	5.321206	95-97
CACAT	237650	2.1384995	5.767901	7
CTTTG	229955	2.117859	8.395123	9
CGAGA	139335	2.0607512	5.1395297	95-97
CGGAC	92760	1.9644995	6.102003	90-94
ACGAG	130620	1.9318572	5.010444	95-97
AGAGA	187025	1.9316978	5.233242	8
GAGAG	118865	1.8663074	6.2729445	7
GGACT	126885	1.8426174	7.343857	6
ATACA	290185	1.8235615	5.0578804	4
TGGAG	113760	1.7537928	5.4267564	5
GCCAA	113995	1.5881346	5.006072	1
TTGAG	159010	1.5833719	5.885417	9
CACCA	117285	1.5391471	5.274815	7
TATAC	249150	1.5373256	5.6573305	5
ATGGA	143920	1.4595538	5.0597534	4
GAGTC	100315	1.4567692	7.146706	9
AGGAG	92030	1.4449693	5.0396714	5
GTCTT	153310	1.4119675	6.132	1
AAGAC	143400	1.3951625	6.1984916	5
CATGA	142045	1.3569415	5.4053154	9
CCATG	97365	1.3318763	5.7503614	9
TGAGT	132660	1.3209867	5.1901746	8
GTGTT	134570	1.3157285	6.206333	1
TATGA	198635	1.3011417	6.491794	4
GACTT	137020	1.2852235	6.80364	7
GTATG	125785	1.2525277	5.9143853	3
GATTG	125265	1.24735	6.426162	7
TCATA	198405	1.2242149	6.534841	2
GTGTA	120870	1.2035857	11.772524	1
GTTCT	128825	1.1864635	5.118188	1
ACATG	119680	1.1432909	5.9935546	8
TGGAC	77870	1.130824	8.047965	5
ACACC	82395	1.081281	5.134832	6
GTCCA	79025	1.0809996	7.455981	1
AGACT	107485	1.0267935	5.8870234	6
ACACT	113810	1.0241221	5.117812	6
TGTAT	157165	1.0108441	5.183663	2
GAGTA	98990	1.0038997	5.463745	1
TGTAG	98545	0.9812803	5.0170846	2
TGCCG	43360	0.9016542	5.07177	95-97
CATAC	100120	0.9009325	5.6413746	3
GTATA	118120	0.77373505	7.077199	1
>>END_MODULE