##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-135_GGACTCCT-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1864430	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.577412935857073	31.0	31.0	34.0	30.0	34.0
2	31.778171344593254	33.0	31.0	34.0	30.0	34.0
3	31.916338505602248	34.0	31.0	34.0	30.0	34.0
4	35.44537472578751	37.0	35.0	37.0	33.0	37.0
5	35.30921997607848	37.0	35.0	37.0	33.0	37.0
6	35.45614262804182	37.0	35.0	37.0	33.0	37.0
7	35.4637492423958	37.0	35.0	37.0	33.0	37.0
8	35.518299426634414	37.0	35.0	37.0	33.0	37.0
9	37.29984982005224	39.0	37.0	39.0	34.0	39.0
10-14	37.41664755448046	39.2	37.2	39.4	33.4	39.4
15-19	38.44946991842011	40.0	38.0	41.0	33.8	41.0
20-24	38.338244181867914	40.0	38.0	41.0	33.8	41.0
25-29	38.147267100400654	40.0	38.0	41.0	33.0	41.0
30-34	37.87079944004334	40.0	38.0	41.0	32.6	41.0
35-39	37.542579769688324	40.0	37.2	41.0	31.6	41.0
40-44	37.48668075497605	40.0	37.0	41.0	31.6	41.0
45-49	37.30857656227373	40.0	36.8	41.0	31.0	41.0
50-54	36.632252001952345	38.8	35.4	40.2	30.4	40.6
55-59	37.27006355829932	40.0	36.0	41.0	31.0	41.0
60-64	36.82198484255242	39.0	35.2	41.0	31.0	41.0
65-69	36.15518104729059	38.0	35.0	40.2	29.8	41.0
70-74	35.33331109239821	36.6	34.4	39.2	29.0	41.0
75-79	34.39039792322587	35.4	34.0	37.8	28.6	39.6
80-84	33.50532227007718	35.0	33.4	36.4	27.4	38.2
85-89	32.772419238051306	35.0	33.0	35.4	26.0	36.8
90-94	32.24095224814018	35.0	32.4	35.0	25.4	36.0
95-99	31.80282552844569	35.0	32.0	35.0	24.6	35.2
100-101	30.97843415950183	34.0	30.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	6.0
6	48.0
7	191.0
8	453.0
9	536.0
10	675.0
11	947.0
12	1493.0
13	2047.0
14	2680.0
15	3165.0
16	3607.0
17	4146.0
18	4734.0
19	5492.0
20	6183.0
21	6929.0
22	8083.0
23	9385.0
24	10568.0
25	12276.0
26	14189.0
27	17071.0
28	20262.0
29	24145.0
30	29363.0
31	37073.0
32	48195.0
33	64414.0
34	91827.0
35	141087.0
36	240172.0
37	431874.0
38	517491.0
39	103552.0
40	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6288898415439	23.35731842040737	10.710662159022684	24.30312957902604
2	15.106492452950654	23.40639571723124	40.14586008141253	21.341251748405575
3	15.860617434570198	27.583418906318236	32.76238179393167	23.793581865179895
4	11.100506295022408	18.156124710491202	44.35481318266268	26.38855581182371
5	10.730004276184655	39.1965708969418	38.9801326422046	11.09329218466894
6	25.70253315282805	43.73217921514528	19.36530651213583	11.199981119890838
7	24.022321198702436	33.77915658308017	26.602700255736917	15.595821962480477
8	21.27231504719261	38.728457327123586	24.03644963127172	15.962777994412086
9	22.957364151903786	15.007908638464466	25.1387431030719	36.89598410655985
10-14	20.531291225351687	25.8589007173647	32.77058286565898	20.83922519162463
15-19	19.821944619609205	31.634025934255867	28.610891417484734	19.933138028650195
20-24	19.752758842824605	30.00687108834005	30.25585684102465	19.984513227810698
25-29	19.720129432118938	30.495029367239823	29.77988944804693	20.00495175259431
30-34	19.6219849544468	30.288082212779997	29.830914259741814	20.259018573031394
35-39	19.800962649305635	30.536228511794206	29.71189286093207	19.95091597796808
40-44	19.50165725157802	30.280122400801474	30.18107568255012	20.03714466507039
45-49	19.693700888262274	30.15427171140305	30.104386212034186	20.04764118830049
50-54	19.43395105075899	29.829158468088245	30.51353728242523	20.223353198727533
55-59	19.643614009006626	29.811357309310228	30.504141741678087	20.040886940005063
60-64	19.370137703926098	29.472545309907588	30.767158544311098	20.390158441855213
65-69	19.688077310015217	29.635327019720712	30.240399145687125	20.436196524576943
70-74	19.559963559187192	29.404519308834814	30.329423274031715	20.70609385794628
75-79	19.719012310925866	29.661150229856304	30.04120806619038	20.578629393027455
80-84	19.615974959347884	29.38411226152788	30.064070331137867	20.93584244798637
85-89	19.71413938427248	29.349155426933905	30.042753252838956	20.893951935954657
90-94	19.658308637302635	28.88792979890436	30.515389639425077	20.938371924367924
95-99	19.975130281194538	28.807849828179872	30.048900505676414	21.168119384949176
100-101	20.049481384137795	28.13323767826373	30.727564081074377	21.089716856524102
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.93201942056994
2	36.44774420135623
3	39.65419929975009
4	37.48906210684611
5	21.823296460853594
6	36.90251427271888
7	39.618143161182914
8	37.23509304160469
9	59.85334825846363
10-14	41.370516416976315
15-19	39.755082648259396
20-24	39.737272070635306
25-29	39.72508118471325
30-34	39.881003527478185
35-39	39.75187862727372
40-44	39.53880191664841
45-49	39.741342076562766
50-54	39.65730424948652
55-59	39.684500949011685
60-64	39.76029614578131
65-69	40.12427383459216
70-74	40.266057417133474
75-79	40.297641703953325
80-84	40.551817407334255
85-89	40.60809132022714
90-94	40.59668056167055
95-99	41.14324966614371
100-101	41.1391982406619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	4.0
3	6.5
4	10.5
5	12.5
6	19.5
7	30.0
8	36.5
9	58.0
10	83.0
11	103.5
12	138.5
13	195.0
14	273.5
15	395.5
16	567.0
17	811.5
18	1143.5
19	1618.5
20	2389.0
21	3509.5
22	5017.0
23	7141.0
24	9761.0
25	13012.5
26	16983.5
27	21481.5
28	26468.5
29	31785.5
30	37895.5
31	44616.5
32	51583.0
33	58906.0
34	67212.0
35	76872.0
36	87665.5
37	98330.0
38	105637.0
39	107573.5
40	104086.0
41	97320.0
42	89410.0
43	81779.0
44	75310.5
45	68769.0
46	62206.0
47	55940.5
48	49346.0
49	42175.0
50	35819.5
51	31527.5
52	27590.0
53	23655.5
54	20460.0
55	17729.0
56	15707.5
57	13618.0
58	11195.5
59	9457.5
60	8094.0
61	6910.0
62	5868.5
63	4536.5
64	3647.5
65	3355.5
66	2881.0
67	2407.5
68	2159.0
69	2073.0
70	1962.0
71	1669.5
72	1411.0
73	1191.5
74	986.5
75	784.5
76	553.0
77	396.5
78	280.5
79	185.5
80	141.5
81	99.5
82	70.0
83	59.5
84	52.0
85	37.5
86	25.5
87	24.0
88	20.0
89	16.0
90	11.0
91	10.5
92	14.5
93	12.5
94	7.0
95	7.0
96	7.0
97	4.0
98	3.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033951395332621766
2	0.03159142472498297
3	0.02810510450915293
4	0.026656940727192765
5	0.0332004955938276
6	0.0018236136513572513
7	7.508997387941623E-4
8	0.002628149085779568
9	6.972640431660078E-4
10-14	0.007197910353298327
15-19	0.02524095836260948
20-24	0.020789195625472663
25-29	0.0021239735468749164
30-34	0.0020703378512467617
35-39	0.006253922110242809
40-44	0.013902372306817632
45-49	0.008624619857007236
50-54	0.0044195813197599266
55-59	0.003722317276593919
60-64	0.011424403168796898
65-69	0.015479261758285373
70-74	0.01964139173903016
75-79	0.007230091770675219
80-84	0.01058768631699769
85-89	0.00808826290072569
90-94	0.008731891248263544
95-99	0.008302805683238308
100-101	0.004961301845604287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1864430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	22.822314707420798
#Duplication Level	Relative count
1	100.0
2	7.203602095721922
3	2.2907961503780667
4	1.426222455342146
5	1.0242870361093595
6	0.7844223504381803
7	0.6335492311953748
8	0.5033032962240466
9	0.4408323952875724
10++	3.5626093977451543
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	492310	2.6784558	5.0197515	5
GCTGC	229320	2.5566175	5.47726	90-94
ACACA	450405	2.3052876	7.458417	6
CAAAG	428620	2.2618108	5.0198107	4
CGACG	185735	2.1119382	5.566606	95-97
GACGC	185095	2.1046612	5.2375364	95-97
GCCGA	184385	2.0965877	5.364647	90-94
CGCTG	187925	2.0951178	5.0703826	95-97
CTTTG	418515	2.0816324	8.403713	9
CTGCC	192360	2.0800638	5.124783	90-94
TACAC	394645	1.9804547	9.584062	5
CACAT	380105	1.9074882	5.0643196	7
AGAGA	348635	1.8967793	5.013047	8
TGCCG	169935	1.8945527	5.1387844	90-94
GAGAG	226270	1.8348713	6.016734	7
GCTTT	353280	1.757163	5.0115457	1
CACCA	220555	1.6319604	5.091736	7
ATACA	467915	1.6242557	5.068488	6
TGGCT	209420	1.5834615	5.0929785	6
TTGAG	289430	1.5137776	5.222458	9
GACTC	191455	1.4320487	5.0237913	7
AAGAC	269630	1.4228269	5.859432	5
GAGTC	181810	1.4020736	7.2777305	9
CCATG	186500	1.3949862	6.064953	9
GTGTA	264810	1.38501	10.443261	1
TATAC	396835	1.3506218	6.1978908	5
GTCTT	267615	1.3310778	6.57509	1
GACTT	250715	1.2718531	6.438001	7
TGAGT	242725	1.269501	5.3137975	8
GTGTT	246690	1.2650464	6.057537	1
TATGA	359725	1.262282	6.1109123	4
AACAC	246440	1.2613426	5.2437468	5
TCATA	362110	1.2324361	6.008026	2
GATTG	230945	1.2078893	5.8817983	7
GTTCT	235335	1.1705219	5.1756215	1
ACACC	157140	1.1627316	5.576016	6
ACCAT	231455	1.1615151	5.198307	8
ACATG	221845	1.1478097	5.296767	8
TGGAC	142755	1.100891	7.2312064	5
GTCCA	144775	1.0828907	7.917498	1
ACACT	212620	1.066995	5.4559975	6
AGACT	198000	1.0244374	5.419438	6
GGACT	131940	1.0174885	6.918389	6
GAGTA	185355	0.9887501	5.330791	1
GTATG	186090	0.9732885	5.335577	3
GTATA	222245	0.779862	7.5938177	1
>>END_MODULE