##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-135_GGACTCCT-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1864430	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.450671250730785	34.0	31.0	34.0	31.0	34.0
2	32.6358581443122	34.0	31.0	34.0	31.0	34.0
3	32.74347173130662	34.0	31.0	34.0	31.0	34.0
4	36.25235326614568	37.0	37.0	37.0	35.0	37.0
5	35.97860418465697	37.0	35.0	37.0	35.0	37.0
6	36.07329371443283	37.0	36.0	37.0	35.0	37.0
7	36.069763412946585	37.0	36.0	37.0	35.0	37.0
8	36.094037856073975	37.0	36.0	37.0	35.0	37.0
9	37.95071415928729	39.0	38.0	39.0	35.0	39.0
10-14	38.131267250580606	39.2	38.2	39.4	35.2	39.4
15-19	39.267132152990456	40.0	39.0	41.0	36.0	41.0
20-24	39.19391717575881	40.0	39.0	41.0	36.0	41.0
25-29	39.00238603755571	40.0	38.8	41.0	35.8	41.0
30-34	38.79889188652832	40.0	38.0	41.0	35.0	41.0
35-39	38.566987122069484	40.0	38.0	41.0	34.4	41.0
40-44	38.32136942658078	40.0	38.0	41.0	33.8	41.0
45-49	38.13261189747001	40.0	38.0	41.0	33.4	41.0
50-54	38.08337861973901	40.0	37.4	41.0	33.2	41.0
55-59	37.703896847830165	39.6	36.6	41.0	33.0	41.0
60-64	37.15907328245093	39.0	35.4	40.6	31.8	41.0
65-69	36.43196322736708	37.8	35.0	40.0	31.0	41.0
70-74	35.86347323310609	36.6	35.0	39.2	31.0	41.0
75-79	34.69583143373578	35.2	33.6	37.4	30.2	39.2
80-84	34.50508080217547	35.0	34.0	36.6	31.0	38.4
85-89	33.87671728088478	35.0	34.0	35.6	30.2	36.8
90-94	33.4604637342244	35.0	34.0	35.0	30.0	36.0
95-99	33.18575425196977	35.0	34.0	35.0	29.0	35.6
100-101	32.83876600355069	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	1.0
14	13.0
15	53.0
16	164.0
17	318.0
18	519.0
19	916.0
20	1424.0
21	2036.0
22	2809.0
23	4025.0
24	5596.0
25	7264.0
26	9269.0
27	11946.0
28	15491.0
29	19482.0
30	24781.0
31	31778.0
32	41361.0
33	56114.0
34	81259.0
35	129349.0
36	234626.0
37	450658.0
38	599865.0
39	133172.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07056848473796	23.42646277950902	11.077809303647763	24.425159432105254
2	15.337372961455811	23.193405421527114	40.040489261333825	21.42873235568325
3	16.05659638602683	27.251331506143966	32.95146505902608	23.74060704880312
4	11.30522465311114	18.150855757523747	44.092349940732554	26.451569648632557
5	10.583985453999345	39.30413048492032	39.064003475593076	11.047880585487253
6	25.738053989691217	43.96201520035614	19.182270184453156	11.117660625499482
7	23.9124021819001	33.813658866248666	26.631624678856276	15.642314272994964
8	21.297232934462542	38.805854872534766	23.885798876868535	16.011113316134153
9	22.829658394254544	14.872427497948435	25.09319202115392	37.2047220866431
10-14	20.475222990404575	25.830983195936557	32.80334472197937	20.890449091679493
15-19	19.759475497004733	31.693789473639043	28.60385554927653	19.94287948007969
20-24	19.71347415510104	30.026256984618584	30.30567927303005	19.95458958725033
25-29	19.730685796555424	30.51209460201063	29.729609681303586	20.027609920130363
30-34	19.644376863501645	30.247019336057292	29.83113120135103	20.27747259909004
35-39	19.74122062954594	30.513291826067846	29.76430661346044	19.981180930925774
40-44	19.510560900466455	30.24841904712024	30.188671145272938	20.052348907140363
45-49	19.68091146320213	30.110371826345805	30.13063495944261	20.078081751009456
50-54	19.414808726642352	29.830753178086844	30.478637920157535	20.275800175113268
55-59	19.620669046857362	29.765909042860926	30.502309380218563	20.111112530063153
60-64	19.26303344036718	29.4553079850798	30.69868294491259	20.582975629640426
65-69	19.562608645446296	29.644219230547165	30.128488968952432	20.664683155054103
70-74	19.49011821369735	29.48571243445639	30.09111820865543	20.933051143190827
75-79	19.71108059325174	29.71079524816762	29.697783083241596	20.88034107533904
80-84	19.543276151850243	29.51001981091879	29.57680710807356	21.36989692915741
85-89	19.558859277068418	29.477107509881595	29.47181887598186	21.492214337068134
90-94	19.421400498588113	28.994629112469607	29.955289268104874	21.628681120837406
95-99	19.51012003166288	28.998009752673177	29.550329399717246	21.941540815946695
100-101	19.38737308453522	28.224846199642784	30.398620489908446	21.98916022591355
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.49572791684322
2	36.76610531713906
3	39.797203434829946
4	37.756794301743696
5	21.6318660394866
6	36.855714615190706
7	39.554716454895065
8	37.3083462505967
9	60.03438048089764
10-14	41.36567208208407
15-19	39.702354977084426
20-24	39.668063742351364
25-29	39.75829571668578
30-34	39.92184946259169
35-39	39.72240156047172
40-44	39.562909807606815
45-49	39.758993214211586
50-54	39.69060890175562
55-59	39.73178157692052
60-64	39.846009070007604
65-69	40.227291800500396
70-74	40.42316935688818
75-79	40.591421668590776
80-84	40.91317308100765
85-89	41.05107361413655
90-94	41.05008161942552
95-99	41.451660847609574
100-101	41.37653331044877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.5
5	1.0
6	2.5
7	5.5
8	10.0
9	17.0
10	30.5
11	51.0
12	89.5
13	139.0
14	226.5
15	331.5
16	490.0
17	766.0
18	1127.0
19	1630.0
20	2362.0
21	3472.0
22	5092.5
23	7240.5
24	9885.5
25	13150.0
26	17078.5
27	21385.5
28	26306.0
29	31779.0
30	37748.0
31	44183.0
32	50597.5
33	57913.0
34	66257.5
35	75491.5
36	86774.5
37	97425.5
38	104396.0
39	106155.5
40	102535.5
41	95666.5
42	87388.5
43	80241.0
44	74809.0
45	69188.5
46	63099.5
47	57180.0
48	51238.5
49	44336.5
50	37786.5
51	33325.5
52	29325.0
53	25216.5
54	21410.0
55	18580.5
56	16627.5
57	14142.0
58	11499.0
59	9692.5
60	8209.5
61	6986.0
62	5881.5
63	4481.0
64	3599.0
65	3252.0
66	2875.5
67	2412.5
68	2107.0
69	2076.0
70	1980.0
71	1681.0
72	1432.5
73	1195.5
74	969.5
75	764.0
76	542.0
77	387.0
78	243.5
79	155.5
80	105.5
81	69.5
82	45.0
83	29.0
84	16.5
85	10.0
86	7.5
87	5.0
88	2.0
89	1.5
90	1.5
91	2.0
92	2.0
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012443481385731833
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011370767473168743
20-24	6.329012084122225E-4
25-29	0.017023969792376224
30-34	0.0070048218490369706
35-39	0.042286382433236964
40-44	0.05007428543844499
45-49	0.050803730898987896
50-54	0.050975365124997986
55-59	0.041288758494553296
60-64	0.05200517048105856
65-69	0.04286564794602104
70-74	0.00325032315506616
75-79	0.0011156224690656127
80-84	1.9308850426135604E-4
85-89	0.002917781842171602
90-94	0.0024350605815182122
95-99	0.0020703378512467617
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1864430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	23.632947780619748
#Duplication Level	Relative count
1	100.0
2	7.466906948207077
3	2.3682354126776177
4	1.4488028406968956
5	1.0753389333159444
6	0.799094201824638
7	0.6437806403424012
8	0.516921471803475
9	0.445192689592213
10++	3.7956974586670302
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	494465	2.7067144	5.162141	5
CCGAG	228710	2.569967	5.6463604	95-97
CTCCG	237025	2.5104141	5.310048	95-97
GAGCC	222940	2.5051303	5.484723	95-97
CGAGC	210660	2.3671427	5.403843	85-89
GCCCA	213280	2.2891936	5.1960354	90-94
ACACA	451090	2.2529433	7.276708	6
AGCCC	207685	2.229141	5.088033	95-97
CTTTG	418015	2.1001256	8.264958	9
GACTC	263525	1.9480736	5.250789	7
TACAC	393405	1.9388568	9.548492	5
AGAGA	346885	1.8988575	5.02413	8
GACGG	157995	1.8586415	5.106975	95-97
GAGAG	223460	1.8347813	5.7763743	7
GCTTT	351195	1.7644188	5.2086487	1
ATACA	472050	1.6237762	5.071752	6
GGACT	204720	1.5843593	6.566514	6
TTGAG	289095	1.5409378	5.5382185	9
GAGTC	182350	1.4112345	7.0468073	9
AAGAC	269695	1.410165	5.759818	5
GTCTT	279705	1.4052501	6.531519	1
CCATG	185195	1.3690295	5.9174423	9
TATAC	400430	1.3592007	6.2439256	5
GTGTT	248645	1.3078052	6.3252687	1
TGAGT	243370	1.2972138	5.277201	8
TATGA	359995	1.2792733	6.2732267	4
GACTT	249685	1.2712386	6.5514727	7
TCATA	364825	1.2383447	6.0604477	2
AACAC	246710	1.232179	5.0682697	5
GATTG	229740	1.224563	5.8457537	7
GTGTA	224425	1.1962329	11.22014	1
GTTCT	236860	1.1899949	5.169669	1
GTATG	216545	1.1542308	5.548556	3
ACATG	221700	1.1438826	5.163318	8
ACCAT	228885	1.1280366	5.1159463	8
ACACC	155635	1.1136842	5.3116984	6
TGGAC	142780	1.1049962	7.028046	5
GTCCA	145170	1.0731499	7.531362	1
ACACT	213145	1.0504636	5.4146347	6
AGACT	199370	1.0286688	5.7837167	6
GAGTA	185330	1.001086	5.3041368	1
TGTAT	284845	0.9988362	5.114786	2
GTATA	221410	0.78679955	7.499283	1
ATACT	222625	0.7556678	5.0063314	6
>>END_MODULE