##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-134_GGACTCCT-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1908600	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.749054804568793	33.0	31.0	34.0	30.0	34.0
2	31.956655663837367	33.0	31.0	34.0	30.0	34.0
3	32.10184271193545	34.0	31.0	34.0	30.0	34.0
4	35.62484962799958	37.0	35.0	37.0	33.0	37.0
5	35.4991559258095	37.0	35.0	37.0	33.0	37.0
6	35.63355915330609	37.0	35.0	37.0	33.0	37.0
7	35.65647909462433	37.0	35.0	37.0	33.0	37.0
8	35.71335062349366	37.0	35.0	37.0	35.0	37.0
9	37.53047678926962	39.0	38.0	39.0	35.0	39.0
10-14	37.68515162946663	39.2	37.2	39.4	35.0	39.4
15-19	38.75654301582311	40.0	38.2	41.0	35.0	41.0
20-24	38.66796646756785	40.0	38.4	41.0	34.4	41.0
25-29	38.49518694330923	40.0	38.0	41.0	34.0	41.0
30-34	38.239350413915965	40.0	38.0	41.0	33.6	41.0
35-39	37.93413098606308	40.0	38.0	41.0	33.0	41.0
40-44	37.8998326522058	40.0	38.0	41.0	32.8	41.0
45-49	37.753168814838105	40.0	37.4	41.0	32.6	41.0
50-54	37.03929508540291	39.2	36.4	40.2	31.6	40.6
55-59	37.662243110133076	40.0	36.8	41.0	32.6	41.0
60-64	37.217807398092845	39.2	35.6	41.0	31.8	41.0
65-69	36.560181284711305	38.2	35.0	40.4	31.0	41.0
70-74	35.74115110552237	36.6	35.0	39.4	30.4	41.0
75-79	34.800406161584405	35.6	34.0	38.0	29.4	39.8
80-84	33.88482950854029	35.0	34.0	36.6	29.0	38.4
85-89	33.10600586817563	35.0	33.4	35.6	27.4	36.8
90-94	32.51687163365818	35.0	33.0	35.0	26.0	36.0
95-99	31.989200356282094	35.0	32.4	35.0	24.8	35.4
100-101	31.08265508749869	34.5	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	45.0
7	167.0
8	403.0
9	562.0
10	650.0
11	786.0
12	1028.0
13	1401.0
14	1661.0
15	2172.0
16	2454.0
17	2915.0
18	3480.0
19	4130.0
20	4830.0
21	5576.0
22	6446.0
23	7629.0
24	9072.0
25	10431.0
26	12346.0
27	14863.0
28	17636.0
29	22029.0
30	27079.0
31	34370.0
32	44843.0
33	61327.0
34	87608.0
35	137042.0
36	241569.0
37	449122.0
38	563372.0
39	129405.0
40	147.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.208946336968594	23.864568499077777	10.237451169801986	23.689033994151647
2	15.697342876670398	24.11123480550629	40.176935397981474	20.01448691984184
3	16.512447831580978	28.80367588520563	32.86247453489329	21.821401748320103
4	11.658437467080145	20.353000430283803	43.51505705060106	24.47350505203499
5	11.519383030400869	40.576474941637585	37.064771534865976	10.839370493095572
6	25.195892017979887	43.61233526339257	20.118549055133748	11.073223663493795
7	22.809787104951134	34.270585714188414	27.646229447556394	15.273397733304062
8	20.241316362523655	37.41084770912932	25.772792910991278	16.575043017355746
9	22.02999269620947	17.281638144858164	25.79951356964641	34.88885558928595
10-14	20.140889911325772	26.717209105016543	32.69216662069613	20.449734362961554
15-19	19.401886548411326	32.031283573629246	29.154451324749246	19.412378553210186
20-24	19.268779643746274	30.633929807633553	30.786345553179963	19.31094499544021
25-29	19.288886308702075	30.951095516350225	30.340897635349602	19.4191205395981
30-34	19.15710317878234	30.79476107915859	30.51005443368066	19.53808130837841
35-39	19.283039838816688	30.96355885143266	30.430329726389317	19.32307158336134
40-44	19.02644034515421	30.686074377055846	30.810150721474756	19.47733455631519
45-49	19.06602047196135	30.440981146739198	30.936347949146437	19.556650432153013
50-54	18.813960984794818	30.159655672244778	31.20519534849017	19.821187994470232
55-59	18.86842259143343	30.146492171022942	31.220798132505667	19.764287105037965
60-64	18.684281514219307	29.76690694408085	31.311998101870607	20.236813439829234
65-69	18.97900946965489	29.713468424073184	30.900445164749524	20.407076941522398
70-74	18.96714232067127	29.440826966981753	30.868763703102104	20.723267009244875
75-79	19.237508874980023	29.577034826600574	30.56345577967518	20.62200051874422
80-84	19.376088481593197	29.327165629039392	30.543183886081287	20.753562003286117
85-89	19.662105748207665	29.097257569750738	30.44492979941537	20.795706882626224
90-94	19.89412984567493	28.71599295163123	30.677151048839946	20.712726153853893
95-99	20.339229239008343	28.337833739358476	30.308542080141255	21.014394941491926
100-101	20.7124959653925	27.725141578045026	30.639176143627832	20.92318631293464
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.89798033112024
2	35.71182979651224
3	38.33384957990108
4	36.131942519115135
5	22.358753523496443
6	36.269115681473686
7	38.0831848382552
8	36.8163593798794
9	56.91884828549543
10-14	40.59062427428732
15-19	38.814265101621515
20-24	38.57972463918649
25-29	38.70800684830017
30-34	38.695184487160745
35-39	38.606111422178024
40-44	38.5037749014694
45-49	38.622670904114365
50-54	38.63514897926505
55-59	38.63270969647139
60-64	38.92109495404854
65-69	39.38608641117728
70-74	39.69040932991614
75-79	39.85950939372424
80-84	40.12965048487931
85-89	40.45781263083389
90-94	40.606855999528825
95-99	41.35362418050027
100-101	41.63568227832714
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	8.5
3	14.0
4	19.0
5	21.5
6	27.5
7	40.0
8	63.5
9	89.5
10	114.0
11	166.5
12	251.5
13	366.0
14	534.5
15	822.0
16	1222.0
17	1767.5
18	2594.5
19	3679.0
20	5209.5
21	7246.0
22	9716.5
23	12758.5
24	16044.5
25	19462.5
26	23372.0
27	27782.5
28	32720.5
29	37938.5
30	43352.0
31	49137.5
32	55080.0
33	61540.5
34	68598.0
35	76884.5
36	85983.0
37	93580.0
38	98297.5
39	99708.5
40	98021.5
41	94149.0
42	88063.5
43	81811.5
44	76626.0
45	70428.0
46	64020.0
47	57467.5
48	50619.5
49	43498.0
50	36800.0
51	32230.0
52	28371.5
53	24602.5
54	21218.5
55	18354.0
56	16090.0
57	13502.5
58	10942.0
59	9141.5
60	7519.0
61	6069.5
62	4884.5
63	3539.5
64	2515.0
65	2014.5
66	1627.5
67	1258.0
68	1071.5
69	1049.5
70	970.5
71	827.5
72	687.0
73	562.5
74	459.0
75	338.5
76	235.5
77	170.5
78	132.0
79	102.0
80	78.0
81	62.0
82	43.0
83	32.0
84	26.5
85	22.0
86	17.5
87	15.5
88	11.0
89	10.5
90	12.0
91	9.0
92	9.5
93	7.0
94	5.0
95	6.5
96	8.0
97	8.5
98	5.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03735722519123965
2	0.03520905375668029
3	0.03127947186419365
4	0.02918369485486744
5	0.0363617311118097
6	0.0012050717803625695
7	3.6676097663208634E-4
8	0.003562820915854553
9	1.047888504663104E-4
10-14	0.006874148590589961
15-19	0.02517028188200775
20-24	0.020863460127842397
25-29	0.0020329036990464214
30-34	0.0020433825840930524
35-39	0.006004401131719584
40-44	0.013559677250340563
45-49	0.008152572566278948
50-54	0.004862202661636802
55-59	0.003919103007440008
60-64	0.010206434035418632
65-69	0.014293199203604737
70-74	0.01908204966991512
75-79	0.007597191658807503
80-84	0.010877082678403018
85-89	0.008833700094309964
90-94	0.00978727863355339
95-99	0.009410038771874673
100-101	0.005868175626113381
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1908600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.17016568597787
#Duplication Level	Relative count
1	100.0
2	7.353921622596962
3	2.764678178003682
4	1.9511777406707402
5	1.566447082714033
6	1.227590509569334
7	0.9792587971203308
8	0.8128887828687817
9	0.6868879632517998
10++	4.98804215522567
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	530175	3.89188	6.963754	90-94
GCTGC	327770	3.785322	9.548638	90-94
CTGTC	500005	3.6704097	6.5692835	90-94
GACGC	300270	3.558641	9.525047	95-97
CGCTG	297745	3.4385715	9.282028	90-94
TCTCT	731375	3.4126122	5.2294564	95-97
GCCGA	279305	3.310175	9.674505	95-97
CTGCC	294540	3.2960956	9.00076	90-94
CGACG	273925	3.246414	9.670673	95-97
CTCTT	676430	3.1562376	4.985033	95-97
TGCCG	268520	3.1010604	9.214501	95-97
CCGAC	266480	3.0602632	9.30957	95-97
TGTCT	609145	2.9332263	5.02062	90-94
ACACA	564670	2.8474588	5.8812466	6
CTGAC	351440	2.6474638	6.592771	95-97
TGACG	335110	2.6052196	6.53222	95-97
TACAC	511710	2.5144794	6.6410336	5
GACGA	308195	2.4587884	6.610595	95-97
AGAAG	457035	2.45453	5.12192	5
ATCTG	494215	2.4421902	5.165153	95-97
ACGCT	306080	2.3057585	5.9136114	90-94
TGCAG	261100	2.0298493	5.7740417	90-94
GGTGG	147625	1.8157233	5.641573	95-97
CTTTG	373885	1.8003749	6.919131	9
GAGAG	216050	1.7788032	5.573937	7
GTGTG	217110	1.6973686	5.490821	95-97
GCAGT	211675	1.6456084	5.525426	95-97
CAGTG	208035	1.6173103	5.651118	95-97
CTCGG	129685	1.497695	5.9189415	95-97
GTGTA	271140	1.3827231	9.22508	1
GAGTC	161870	1.2584133	6.9709	9
AAGAC	239430	1.246004	5.2256656	5
CGGTG	104305	1.2431307	5.1487203	95-97
GTCTT	241255	1.1617193	5.5104737	1
TGAGT	216535	1.1042559	5.003167	8
GTGTT	221490	1.1006699	5.6940193	1
TGGAC	133145	1.0350988	5.690843	5
GTCCA	134400	1.0124606	7.177223	1
GGACT	119350	0.9278534	5.161215	6
GAGTA	169660	0.8878911	5.455729	1
GTATA	211745	0.70436865	6.9098663	1
>>END_MODULE