##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-134_GGACTCCT-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1908600	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.479780467358275	34.0	31.0	34.0	31.0	34.0
2	32.66922351461805	34.0	31.0	34.0	31.0	34.0
3	32.780208005868175	34.0	31.0	34.0	31.0	34.0
4	36.282697265011	37.0	37.0	37.0	35.0	37.0
5	36.012313213874044	37.0	35.0	37.0	35.0	37.0
6	36.0970674840197	37.0	36.0	37.0	35.0	37.0
7	36.104350309127106	37.0	36.0	37.0	35.0	37.0
8	36.13181808655559	37.0	36.0	37.0	35.0	37.0
9	38.00418945824164	39.0	38.0	39.0	35.0	39.0
10-14	38.21525296028502	39.4	38.2	39.4	35.2	39.4
15-19	39.36306884627476	41.0	39.0	41.0	36.0	41.0
20-24	39.30091679765273	40.6	39.0	41.0	36.0	41.0
25-29	39.11382626008593	40.0	39.0	41.0	36.0	41.0
30-34	38.923046945405005	40.0	38.4	41.0	35.4	41.0
35-39	38.70067777428481	40.0	38.0	41.0	35.0	41.0
40-44	38.469418421879915	40.0	38.0	41.0	34.0	41.0
45-49	38.29852142931992	40.0	38.0	41.0	33.8	41.0
50-54	38.24329707639107	40.0	37.8	41.0	34.0	41.0
55-59	37.870268888190296	40.0	36.8	41.0	33.0	41.0
60-64	37.32637273394111	39.0	35.6	40.8	32.6	41.0
65-69	36.60734569841769	37.8	35.0	40.0	31.6	41.0
70-74	36.02134957560516	36.8	35.0	39.4	31.0	41.0
75-79	34.811204652624966	35.2	34.0	37.4	30.4	39.2
80-84	34.59013653987216	35.0	34.0	36.6	31.0	38.4
85-89	33.9634156973698	35.0	34.0	35.8	30.6	36.8
90-94	33.54097516504244	35.0	34.0	35.0	30.0	36.0
95-99	33.264808969925596	35.0	34.0	35.0	29.4	35.6
100-101	32.90837289112439	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	10.0
14	19.0
15	72.0
16	137.0
17	298.0
18	580.0
19	956.0
20	1482.0
21	2052.0
22	2945.0
23	3964.0
24	5363.0
25	6982.0
26	9105.0
27	11518.0
28	14787.0
29	18727.0
30	23754.0
31	30105.0
32	38866.0
33	52923.0
34	75881.0
35	122600.0
36	228956.0
37	460774.0
38	639868.0
39	155629.0
40	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00780676935974	23.746882531698628	10.439065283453841	23.806245415487794
2	15.947804479546082	24.05276145052068	39.96095082539328	20.038483244539954
3	16.728701666142722	28.581787697788958	33.025463690663315	21.66404694540501
4	11.865555904851725	20.42324216703343	43.20381431415697	24.507387613957874
5	11.453002200565859	40.586188829508544	37.08341192497118	10.877397044954416
6	25.099025463690662	43.71502672115687	20.119406895106362	11.066540920046107
7	22.69427852876454	34.30556428795976	27.641988892381853	15.358168290893847
8	20.209368123231688	37.41250130986063	25.752541129623808	16.62558943728387
9	21.96709630095358	17.319920360473645	25.730430682175413	34.98255265639736
10-14	20.101802368228018	26.701163156240177	32.68850466310384	20.50852981242796
15-19	19.361156863538216	32.02905907278139	29.154724986288244	19.455059077392146
20-24	19.23149227868656	30.618375728392184	30.80297300537277	19.347158987548486
25-29	19.293655806083706	30.947274359592992	30.35471695107424	19.404352883249064
30-34	19.172764736285476	30.795555482897107	30.448786066010474	19.58289371480694
35-39	19.27151632652612	30.940124312208546	30.41039634252772	19.377963018737614
40-44	19.00192793816088	30.681601468793435	30.798657478180562	19.517813114865127
45-49	19.049634514734056	30.428193502276805	30.936982374238337	19.585189608750802
50-54	18.76640845094787	30.124094308025594	31.20615960182631	19.903337639200227
55-59	18.766865501501037	30.094192590831415	31.188407930204537	19.95053397746301
60-64	18.52429890452948	29.77248274848338	31.149647483823134	20.553570863164005
65-69	18.72061048215216	29.799249163145042	30.634154165823606	20.845986188879188
70-74	18.716826521135978	29.604937334938203	30.34593788426219	21.332298259663627
75-79	18.89743151504952	29.674113523373435	29.951158263510646	21.477296698066397
80-84	18.780912788631134	29.419732376985962	29.72724651094166	22.072108323441242
85-89	18.72597509594243	29.120169528504608	29.75707509256812	22.396780282984842
90-94	18.733212389885015	28.62716122141705	29.968524802908007	22.671101585789927
95-99	18.749234368730537	28.26523594437218	29.862485550592755	23.12304413630453
100-101	18.76606872370781	27.688529649234855	30.330703306456723	23.214698320600608
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.81405218484753
2	35.98628772408603
3	38.39274861154773
4	36.3729435188096
5	22.330399245520276
6	36.16556638373677
7	38.052446819658385
8	36.83495756051556
9	56.94964895735094
10-14	40.61033218065598
15-19	38.81621594093036
20-24	38.57865126623505
25-29	38.698008689332774
30-34	38.755658451092415
35-39	38.649479345263735
40-44	38.51974105302601
45-49	38.634824123484854
50-54	38.6697460901481
55-59	38.71739947896405
60-64	39.077869767693485
65-69	39.56659667103135
70-74	40.0491247807996
75-79	40.374728213115915
80-84	40.853021112072376
85-89	41.122755378927266
90-94	41.40431397567494
95-99	41.87227850503506
100-101	41.980767044308415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.5
6	5.0
7	14.0
8	23.5
9	39.5
10	65.5
11	94.5
12	171.0
13	315.5
14	501.0
15	766.0
16	1130.0
17	1667.0
18	2509.0
19	3593.0
20	5151.0
21	7157.0
22	9568.5
23	12603.5
24	15837.5
25	19387.5
26	23320.5
27	27516.0
28	32651.5
29	37769.0
30	42910.0
31	48465.5
32	53912.5
33	60222.0
34	67410.5
35	75121.5
36	84025.0
37	91755.5
38	96046.5
39	97011.5
40	95090.0
41	91100.0
42	85238.0
43	79945.5
44	75640.5
45	70500.5
46	65521.5
47	60315.5
48	53867.5
49	46707.0
50	40285.5
51	35394.0
52	30708.0
53	26401.0
54	22987.0
55	20007.0
56	17385.0
57	14482.5
58	11673.0
59	9747.0
60	7887.5
61	6393.0
62	5136.0
63	3517.0
64	2481.0
65	2040.0
66	1582.0
67	1220.5
68	1007.5
69	972.5
70	973.0
71	840.0
72	716.0
73	581.5
74	461.5
75	354.0
76	228.5
77	158.0
78	112.0
79	68.0
80	41.0
81	31.5
82	23.0
83	11.5
84	8.0
85	6.5
86	4.0
87	3.0
88	2.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012417478780257781
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.850151943833175E-4
20-24	6.706486429843865E-4
25-29	0.01724824478675469
30-34	0.006664570889657341
35-39	0.04142303258933249
40-44	0.05062349366027455
45-49	0.05091690244158022
50-54	0.05083307136120716
55-59	0.04115058157812009
60-64	0.05237346746306193
65-69	0.045163994550979775
70-74	0.003793356386880436
75-79	0.0014356072513884524
80-84	2.514932411191449E-4
85-89	0.0031227077438960495
90-94	0.0025987634915644975
95-99	0.0023158335953054597
100-101	5.23944252331552E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1908600.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.653700877441764
#Duplication Level	Relative count
1	100.0
2	7.412886158152211
3	2.840675427666509
4	1.9899459240481465
5	1.555987944929689
6	1.272411443723568
7	1.0244354556558781
8	0.8034667534173423
9	0.6635199086662698
10++	5.197061116260228
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	347355	4.0342007	9.699199	95-97
GAGCC	334040	3.8795595	9.5398855	90-94
GTCTC	530900	3.8169951	6.7318892	90-94
CTCCG	358030	3.7983513	9.137658	95-97
CGAGC	321755	3.7368803	9.306737	90-94
CTGTC	501655	3.6067333	6.432074	90-94
AGCCC	313640	3.3899255	8.7093	90-94
GCCCA	311305	3.364688	8.792106	90-94
TCTCT	735560	3.3352993	5.08469	95-97
TCTCC	470265	3.1464891	6.4982924	95-97
CTCTT	675410	3.062557	4.7886086	95-97
CCCAC	298170	2.9991446	8.205031	95-97
TGTCT	610865	2.9763713	5.029474	90-94
CCACG	274820	2.9703455	8.664705	95-97
GACGG	233605	2.915354	9.222079	95-97
ATCTC	609735	2.816701	7.1513476	95-97
TCCGA	383475	2.8088517	6.3053093	95-97
ACACA	565265	2.7103014	5.828093	6
GAGAC	319135	2.5590243	6.6654572	95-97
CGAGA	315100	2.526669	6.6382403	95-97
AGAAG	456115	2.5251684	5.42755	5
CGGAC	217075	2.521121	8.482275	90-94
TACAC	512750	2.4131718	6.790819	5
ACGAG	298595	2.3943217	6.646017	95-97
GACTC	302040	2.212362	5.7690144	95-97
CACGA	288090	2.149824	6.183744	95-97
GGACT	262695	2.0676107	6.424241	95-97
AGACG	256000	2.0527685	6.2248087	95-97
CTCCT	303005	2.0273716	5.582324	95-97
ACGGA	246535	1.9768718	5.9839005	90-94
ACTCC	278840	1.9007351	5.337941	95-97
GAGAG	218410	1.8819052	5.8361745	7
CTTTG	371770	1.811408	6.848521	9
GTCTT	265830	1.2952272	5.315967	1
GAGTC	161975	1.2748674	6.8995714	9
AAGAC	243760	1.2558947	5.488169	5
TGAGT	217170	1.1583772	5.211129	8
GTGTT	219105	1.147148	5.884891	1
GCCGT	96070	1.0951883	5.997434	95-97
TGCCG	92670	1.0564287	6.423058	95-97
TGGAC	132300	1.0413023	5.8425016	5
GTGTA	188450	1.0051856	9.681169	1
GTCCA	135330	0.9912561	6.6631947	1
GAGTA	171075	0.92965037	5.538902	1
GTATA	211585	0.7251466	7.046357	1
>>END_MODULE