##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-133_GGACTCCT-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1307432	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57768893525629	31.0	31.0	34.0	30.0	34.0
2	31.784062957002735	33.0	31.0	34.0	30.0	34.0
3	31.923031561106047	34.0	31.0	34.0	30.0	34.0
4	35.4493618023729	37.0	35.0	37.0	33.0	37.0
5	35.317467371152	37.0	35.0	37.0	33.0	37.0
6	35.460695470204186	37.0	35.0	37.0	33.0	37.0
7	35.470910915443405	37.0	35.0	37.0	33.0	37.0
8	35.51863576843767	37.0	35.0	37.0	33.0	37.0
9	37.30774525940929	39.0	37.0	39.0	34.0	39.0
10-14	37.4278994242148	39.2	37.2	39.4	33.6	39.4
15-19	38.45960952462538	40.0	38.0	41.0	34.0	41.0
20-24	38.35162455867685	40.0	38.0	41.0	33.8	41.0
25-29	38.16487541990712	40.0	38.0	41.0	33.0	41.0
30-34	37.89381030906388	40.0	38.0	41.0	32.6	41.0
35-39	37.576142698052365	40.0	37.2	41.0	31.6	41.0
40-44	37.52381523475026	40.0	37.0	41.0	31.8	41.0
45-49	37.35055436917561	40.0	36.8	41.0	31.0	41.0
50-54	36.65899075439488	38.8	35.4	40.2	30.6	40.6
55-59	37.289620569176826	39.8	36.0	41.0	31.0	41.0
60-64	36.82839183988155	39.0	35.2	41.0	31.0	41.0
65-69	36.15753064021685	37.8	35.0	40.0	30.0	41.0
70-74	35.32026093900104	36.6	34.4	39.2	29.0	41.0
75-79	34.37268416254153	35.4	34.0	37.6	28.6	39.4
80-84	33.47453603705584	35.0	33.4	36.4	27.4	38.0
85-89	32.73533919928532	35.0	33.0	35.4	26.0	36.6
90-94	32.192349888942594	35.0	32.4	35.0	25.2	36.0
95-99	31.73015040170349	34.8	32.0	35.0	24.2	35.2
100-101	30.881495557703957	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	28.0
7	126.0
8	275.0
9	383.0
10	475.0
11	674.0
12	976.0
13	1389.0
14	1744.0
15	2152.0
16	2536.0
17	2903.0
18	3340.0
19	3728.0
20	4213.0
21	4773.0
22	5530.0
23	6473.0
24	7325.0
25	8668.0
26	10063.0
27	11808.0
28	13973.0
29	17027.0
30	20521.0
31	26360.0
32	33936.0
33	45564.0
34	65096.0
35	99040.0
36	171217.0
37	307247.0
38	358963.0
39	68846.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62235405913686	23.60072899658914	10.575041201161131	24.201875743112865
2	15.283781653922723	23.496625162774116	40.09545334216764	21.124139841135523
3	15.784879272883067	27.7441319582581	32.63602839917985	23.834960369678978
4	11.190949618348643	18.362032097426102	44.15710402476354	26.289914259461717
5	10.848519950390852	39.128947825468224	38.72428591211897	11.298246312021956
6	25.672991389840515	43.67883347904137	19.40127381714521	11.246901313972904
7	23.97917116025632	33.74314870989997	26.72649451055439	15.551185619289317
8	21.106322894102387	38.44119824748198	24.354279105628372	16.098199752787263
9	22.873281450178787	15.08897259881064	25.317092758666842	36.720653192343725
10-14	20.512973887310537	25.96962048321884	32.702291842054414	20.8151137874162
15-19	19.76771834625402	31.684837642456277	28.645933586860956	19.901510424428746
20-24	19.746112881356243	30.038535344772455	30.293943313599	19.921408460272303
25-29	19.733459140105207	30.589785022477407	29.76338261122174	19.91337322619565
30-34	19.558654424370534	30.312548139370772	29.855427765205768	20.273369671052922
35-39	19.783048748249897	30.56960259259622	29.707907638759707	19.939441020394177
40-44	19.475070563956287	30.326689430927644	30.144980263205202	20.053259741910868
45-49	19.687640219446443	30.138990602036614	30.098801691208116	20.074567487308826
50-54	19.42659500646802	29.766126298346464	30.55480255098232	20.252476144203197
55-59	19.575777583528716	29.794974769208554	30.547936362048166	20.08131128521457
60-64	19.320092251557604	29.37269705766487	30.829063065337202	20.478147625440318
65-69	19.680579662694694	29.55680007466095	30.29000729015187	20.47261297249249
70-74	19.528541895147075	29.18775520445733	30.36207820884415	20.921624691551447
75-79	19.670224867959604	29.424890723607678	30.006198907205476	20.898685501227238
80-84	19.57171877022327	29.190047252266766	29.93554867919771	21.30268529831225
85-89	19.693892350123704	29.133817913006975	29.860350853144574	21.311938883724743
90-94	19.620407702426125	28.627245375689558	30.33337443627519	21.418972485609125
95-99	19.99927484149779	28.46490370537636	29.808894726873792	21.726926726252053
100-101	20.099706273807886	27.78294093349856	30.516468554469377	21.60088423822418
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.82422980224973
2	36.40792149505825
3	39.61983964256205
4	37.480863877810364
5	22.146766262412807
6	36.919892703813424
7	39.53035677954564
8	37.20452264688965
9	59.59393464252252
10-14	41.32808767472674
15-19	39.669228770682764
20-24	39.66752134162855
25-29	39.646832366300856
30-34	39.832024095423456
35-39	39.722489768644074
40-44	39.52833030586716
45-49	39.76220770675527
50-54	39.67907115067122
55-59	39.65708886874329
60-64	39.79823987699792
65-69	40.15319263518718
70-74	40.45016658669852
75-79	40.56891036918684
80-84	40.874404068535526
85-89	41.00583123384845
90-94	41.03938018803525
95-99	41.72620156774984
100-101	41.700590512032065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.5
2	6.0
3	10.0
4	8.0
5	6.5
6	13.5
7	20.0
8	27.5
9	42.0
10	50.5
11	62.0
12	91.0
13	132.5
14	189.5
15	257.5
16	359.5
17	504.5
18	756.5
19	1133.0
20	1645.0
21	2405.5
22	3505.0
23	4971.0
24	6756.5
25	9017.5
26	11698.0
27	14553.5
28	17975.0
29	21796.5
30	25842.0
31	30280.5
32	34985.5
33	40085.5
34	45904.0
35	53197.0
36	61858.0
37	69580.0
38	74831.0
39	75809.5
40	72988.5
41	68333.0
42	62263.5
43	56537.5
44	52170.5
45	47790.5
46	43448.5
47	39231.5
48	34599.5
49	29854.0
50	25643.5
51	22798.0
52	20400.5
53	18112.5
54	15946.5
55	14055.5
56	12565.5
57	10736.0
58	8832.5
59	7528.5
60	6222.5
61	5086.0
62	4292.5
63	3273.0
64	2420.5
65	2015.0
66	1637.5
67	1317.0
68	1090.5
69	1013.5
70	1001.5
71	835.0
72	673.0
73	579.0
74	453.5
75	331.0
76	246.5
77	183.5
78	134.0
79	86.0
80	64.5
81	58.0
82	37.0
83	27.5
84	20.0
85	15.0
86	15.0
87	15.5
88	15.0
89	9.5
90	5.0
91	4.0
92	5.0
93	6.0
94	9.0
95	10.5
96	7.0
97	4.5
98	2.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03288889976687124
2	0.031053240244999357
3	0.026923006320787618
4	0.026693548880553634
5	0.031741612565701315
6	0.0017591737084605547
7	0.0010708013877585986
8	0.004283205551034394
9	5.354006938792993E-4
10-14	0.006929614695066358
15-19	0.025179129775009332
20-24	0.020528792319600562
25-29	0.0023098715650221196
30-34	0.0023098715650221196
35-39	0.005996487771448152
40-44	0.013629771949898733
45-49	0.008122793384283084
50-54	0.005874110469990026
55-59	0.004680931780773302
60-64	0.010478556437352
65-69	0.014455818734741081
70-74	0.01872372712309321
75-79	0.008015713245507223
80-84	0.011534060662428333
85-89	0.009728995465920981
90-94	0.011105740107324893
95-99	0.010065533044930826
100-101	0.007036694833842219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1307432.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.88058968270727
#Duplication Level	Relative count
1	100.0
2	7.481086794864704
3	3.257188861643221
4	2.256678773576209
5	1.6513332964594971
6	1.369248449762471
7	1.0361547963642397
8	0.7909437858367595
9	0.687697044562031
10++	4.38245542875053
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	176410	2.779683	6.4880786	90-94
AGAAG	343610	2.690567	5.0479236	5
ACACA	334155	2.4218616	7.535515	6
GACGC	148500	2.3879006	6.0533633	85-89
CGCTG	149155	2.3502276	5.9026995	90-94
GCCGA	145845	2.345208	6.242135	90-94
CTGCC	152450	2.3110597	5.9641423	90-94
CAAAG	304410	2.2932353	5.0282617	4
CGACG	140015	2.2514603	6.460504	95-97
GACTC	209070	2.2116663	5.1659174	7
TACAC	299835	2.129443	9.411831	5
TGCCG	134200	2.114582	5.919511	90-94
CTTTG	296560	2.1020794	8.638185	9
CCGAC	135770	2.1004157	5.840346	95-97
CACAT	289485	2.0559366	5.3038898	7
GCTTT	254360	1.8029572	5.4464245	1
ATACA	357670	1.7725981	5.037393	6
GAGAG	144250	1.682434	5.508981	7
TTGAG	200530	1.5077244	5.290582	9
TATAC	305975	1.4859222	5.747906	5
GAGTC	132120	1.4527293	7.321164	9
GTGTA	191465	1.4395674	10.668127	1
AAGAC	186180	1.4025643	5.6641035	5
CCATG	130715	1.3827807	5.925204	9
TATGA	262485	1.3249605	6.1556435	4
GTCTT	182645	1.2946261	6.4951363	1
GACTT	177800	1.2861334	6.51062	7
TCATA	263375	1.2790416	6.405146	2
TGAGT	169900	1.2774267	5.498519	8
GATTG	164090	1.233743	5.943199	7
AACAC	169040	1.2251544	5.1786175	5
GTGTT	166045	1.2233499	5.8111906	1
GTTCT	164075	1.1629981	5.432671	1
ACACC	109060	1.1327205	5.479904	6
ACCAT	159350	1.1317115	5.152488	8
TGGAC	102215	1.123908	7.5151362	5
ACATG	151375	1.1174449	5.4307437	8
GTCCA	103785	1.0978992	7.748623	1
GGACT	96365	1.0595843	7.129095	6
ACACT	147730	1.0491858	5.2280803	6
AGACT	134955	0.99623317	5.298105	6
GTATG	131100	0.9857011	5.4596624	3
GAGTA	128150	0.9832838	5.136403	1
CATAC	130380	0.9259652	5.136446	3
GTATA	153305	0.7738463	7.5000663	1
>>END_MODULE